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Pacific Biosciences exonuclease digestion
Exonuclease Digestion, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exonuclease digestion/product/Pacific Biosciences
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
exonuclease digestion - by Bioz Stars, 2021-07
86/100 stars

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Article Title: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations
Article Snippet: Due to the exonuclease digestion, PacBio large-insert library preparations usually give rise to low yields (~20-30%).

Ligation:

Article Title: Novel recA-Independent Horizontal Gene Transfer in Escherichia coli K-12
Article Snippet: Further DNA purification was achieved with MO BIO PowerClean DNA Clean-Up Kit (MO BIO Laboratories, Carlsbad, CA, USA) and AxyPrep Mag PCR Clean-up kit (Axygen, Union City, CA, USA). .. SMRTbell template libraries were prepared using the 10 kb preparation protocols provided by Pacific Biosciences ( http://www.pacificbiosciences.com/samplenet/ProcedureChecklistLowInput10kbLibraryPreparationandSequencingMagBeadStation.pdf ) and the reagents supplied in the SMRTbell Template Prep Kit 1.0 for DNA repair, ligation, and exonuclease digestion (Pacific Biosciences, Menlo Park, CA, USA). .. Products were verified for size using the Agilent 2100 Bioanalyzer and DNA 12000 Kits (Agilent Technologies, Santa Clara, CA, USA).

Purification:

Article Title: Characterization of the human ESC transcriptome by hybrid sequencing
Article Snippet: Sequencing templates were prepared using the manufacturer’s SMRTbell template protocol (Pacific Biosciences Large Template Prep kit), with a minimum of 1 µg of dsDNA input into each library procedure. .. After exonuclease digestion of incomplete SMRTbell templates, the libraries were purified by three sequential 0.5× AMPure PB purifications (Pacific Biosciences.) ..

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    Pacific Biosciences exonuclease digestion
    Exonuclease Digestion, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease digestion/product/Pacific Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease digestion - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Pacific Biosciences large insert library preparations
    Large Insert Library Preparations, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/large insert library preparations/product/Pacific Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    large insert library preparations - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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