exonuclease digestion  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Millipore exonuclease digestion
    Exonuclease Digestion, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease digestion/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease digestion - by Bioz Stars, 2020-07
    94/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Biochemical and Structural Insights into Xylan Utilization by the Thermophilic Bacterium Caldanaerobius polysaccharolyticus *
    Article Snippet: .. The resultant amplicons were then digested with the exonuclease activity of T4 DNA polymerase and subcloned into pET46 Ek/LIC vector using the Ek/LIC cloning kit (Novagen) and E. coli JM109 as the competent cells by electroporation (Gene Pulser XcellTM from Bio-Rad). .. All recombinant plasmids (pET46- xyl3A , pET46- agu67A , and pET46- xbp1 ) were then extracted using QIAprep spin miniprep kit, and the nucleotides were sequenced (W. M. Keck Center for Comparative and Functional Genomics, University of Illinois) to confirm the integrity of the coding sequence.

    Amplification:

    Article Title: MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair
    Article Snippet: .. The E. coli uvrD , ssb , and exoI(xonA) genes were amplified by PCR (Supplementary Table ), digested with XbaI , and XhoI (for UvrD ), NdeI and EcoRI (for SSB ) or NdeI and BamHI (for ExoI ), and inserted into pET-29a (Novagen) bacterial expression plasmid. .. Hexa-histidine (his6 ) and Formylglycine Generating Enzyme (FGE) recognition hexa-amino acid sequence (LCTPSR; ald6 ) were introduced onto the C-terminus of EcUvrD and EcExoI proteins.

    Filtration:

    Article Title: Ancient mitochondrial M haplogroups identified in the Southwest Pacific
    Article Snippet: .. Samples were prepared for sequencing by an ExoI digest followed by filtration through a Millipore 96-well filter plate (Millipore, Billerica MA) to remove single-stranded DNA and unincorporated nucleotides. .. PCR product was sequenced by using various versions of the BigDye Terminator Sequencing kits from Applied Biosystems on an Applied Biosystems 377XL automated sequencer using described conditions ( ).

    Electroporation:

    Article Title: Biochemical and Structural Insights into Xylan Utilization by the Thermophilic Bacterium Caldanaerobius polysaccharolyticus *
    Article Snippet: .. The resultant amplicons were then digested with the exonuclease activity of T4 DNA polymerase and subcloned into pET46 Ek/LIC vector using the Ek/LIC cloning kit (Novagen) and E. coli JM109 as the competent cells by electroporation (Gene Pulser XcellTM from Bio-Rad). .. All recombinant plasmids (pET46- xyl3A , pET46- agu67A , and pET46- xbp1 ) were then extracted using QIAprep spin miniprep kit, and the nucleotides were sequenced (W. M. Keck Center for Comparative and Functional Genomics, University of Illinois) to confirm the integrity of the coding sequence.

    Buffer Exchange:

    Article Title: Sequence-Specific Capture of Protein-DNA Complexes for Mass Spectrometric Protein Identification
    Article Snippet: .. Buffer exchange and exonuclease digestion The cross-linked and quenched solution of FoxO1-IGFBP1 complex was desalted three times using a cellulose-based 10,000 molecular weight cut-off Amicon Ultra-0.5 mL centrifugal filter (Millipore, MA, USA) prior to exonuclease III digestion. ..

    Purification:

    Article Title: Two New Xylanases with Different Substrate Specificities from the Human Gut Bacterium Bacteroides intestinalis DSM 17393
    Article Snippet: .. The purified amplicons were digested using the exonuclease activity of T4 DNA polymerase, annealed with a similarly digested pET-46b vector (EMD Chemicals, Darmstadt, Germany), and introduced into E. coli XL10 Gold competent cells by chemical transformation. .. Individual colonies were picked and cultured overnight in lysogeny broth (LB) supplemented with ampicillin (100 μg/ml), and plasmid DNA was purified using a Plasmid Minikit from Qiagen (Valencia, CA).

    Sequencing:

    Article Title: Ancient mitochondrial M haplogroups identified in the Southwest Pacific
    Article Snippet: .. Samples were prepared for sequencing by an ExoI digest followed by filtration through a Millipore 96-well filter plate (Millipore, Billerica MA) to remove single-stranded DNA and unincorporated nucleotides. .. PCR product was sequenced by using various versions of the BigDye Terminator Sequencing kits from Applied Biosystems on an Applied Biosystems 377XL automated sequencer using described conditions ( ).

    Incubation:

    Article Title: Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
    Article Snippet: .. Protein-mediated modulation of the double stranded DNA exonuclease activities of SXT-Exo and lambda-Exo SXT-Exo (2 pmol of trimers), and 2 pmol of the protein indicated in the text [BSA (Sigma), SXT-Bet, SXT-Ssb or lambda-Bet] in 48 μl of exonuclease buffer were incubated at 25°C for 5 mins. ..

    Positron Emission Tomography:

    Article Title: MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair
    Article Snippet: .. The E. coli uvrD , ssb , and exoI(xonA) genes were amplified by PCR (Supplementary Table ), digested with XbaI , and XhoI (for UvrD ), NdeI and EcoRI (for SSB ) or NdeI and BamHI (for ExoI ), and inserted into pET-29a (Novagen) bacterial expression plasmid. .. Hexa-histidine (his6 ) and Formylglycine Generating Enzyme (FGE) recognition hexa-amino acid sequence (LCTPSR; ald6 ) were introduced onto the C-terminus of EcUvrD and EcExoI proteins.

    Article Title: Two New Xylanases with Different Substrate Specificities from the Human Gut Bacterium Bacteroides intestinalis DSM 17393
    Article Snippet: .. The purified amplicons were digested using the exonuclease activity of T4 DNA polymerase, annealed with a similarly digested pET-46b vector (EMD Chemicals, Darmstadt, Germany), and introduced into E. coli XL10 Gold competent cells by chemical transformation. .. Individual colonies were picked and cultured overnight in lysogeny broth (LB) supplemented with ampicillin (100 μg/ml), and plasmid DNA was purified using a Plasmid Minikit from Qiagen (Valencia, CA).

    Activity Assay:

    Article Title: Characterisation of Muta(TM)Mouse ?gt10-lacZ transgene: evidence for in vivo rearrangements
    Article Snippet: .. These custom-made primers (Cortec DNA Service Laboratories Inc., Kingston, Canada) were used to generate polymerase chain reaction (PCR) fragments in reactions involving a DNA polymerase deficient in 3′–5′ exonuclease activity (KOD polymerase; Novagen, Gibbstown, NJ, USA) to minimise proofreading errors. .. Fragments generated, either by PCR or cloning in E.coli , were sequenced in both directions using an ABI Prism® 3100 Genetic Analyzer and BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Biochemical and Structural Insights into Xylan Utilization by the Thermophilic Bacterium Caldanaerobius polysaccharolyticus *
    Article Snippet: .. The resultant amplicons were then digested with the exonuclease activity of T4 DNA polymerase and subcloned into pET46 Ek/LIC vector using the Ek/LIC cloning kit (Novagen) and E. coli JM109 as the competent cells by electroporation (Gene Pulser XcellTM from Bio-Rad). .. All recombinant plasmids (pET46- xyl3A , pET46- agu67A , and pET46- xbp1 ) were then extracted using QIAprep spin miniprep kit, and the nucleotides were sequenced (W. M. Keck Center for Comparative and Functional Genomics, University of Illinois) to confirm the integrity of the coding sequence.

    Article Title: Two New Xylanases with Different Substrate Specificities from the Human Gut Bacterium Bacteroides intestinalis DSM 17393
    Article Snippet: .. The purified amplicons were digested using the exonuclease activity of T4 DNA polymerase, annealed with a similarly digested pET-46b vector (EMD Chemicals, Darmstadt, Germany), and introduced into E. coli XL10 Gold competent cells by chemical transformation. .. Individual colonies were picked and cultured overnight in lysogeny broth (LB) supplemented with ampicillin (100 μg/ml), and plasmid DNA was purified using a Plasmid Minikit from Qiagen (Valencia, CA).

    Molecular Weight:

    Article Title: Sequence-Specific Capture of Protein-DNA Complexes for Mass Spectrometric Protein Identification
    Article Snippet: .. Buffer exchange and exonuclease digestion The cross-linked and quenched solution of FoxO1-IGFBP1 complex was desalted three times using a cellulose-based 10,000 molecular weight cut-off Amicon Ultra-0.5 mL centrifugal filter (Millipore, MA, USA) prior to exonuclease III digestion. ..

    Expressing:

    Article Title: MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair
    Article Snippet: .. The E. coli uvrD , ssb , and exoI(xonA) genes were amplified by PCR (Supplementary Table ), digested with XbaI , and XhoI (for UvrD ), NdeI and EcoRI (for SSB ) or NdeI and BamHI (for ExoI ), and inserted into pET-29a (Novagen) bacterial expression plasmid. .. Hexa-histidine (his6 ) and Formylglycine Generating Enzyme (FGE) recognition hexa-amino acid sequence (LCTPSR; ald6 ) were introduced onto the C-terminus of EcUvrD and EcExoI proteins.

    Polymerase Chain Reaction:

    Article Title: Pyrosequencing as a method for SNP identification in the rhesus macaque (Macaca mulatta)
    Article Snippet: .. The amplicons were digested with ExoI (0.25 U/μl PCR product) at 37°C for 90 minutes to remove any remaining primer, heated to 80°C for 20 minutes and filtered with Montage PCRμ96 plates (Millipore, Billerica, MA). .. They were then submitted to UC Davis Division of Biological Sciences, where they were sequenced in both the forward and reverse directions on an ABI 3730 sequencer using the Big Dye Terminator Cycle Sequencing version 3.1 kit (Applied Biosystems, Foster City, CA).

    Article Title: Characterisation of Muta(TM)Mouse ?gt10-lacZ transgene: evidence for in vivo rearrangements
    Article Snippet: .. These custom-made primers (Cortec DNA Service Laboratories Inc., Kingston, Canada) were used to generate polymerase chain reaction (PCR) fragments in reactions involving a DNA polymerase deficient in 3′–5′ exonuclease activity (KOD polymerase; Novagen, Gibbstown, NJ, USA) to minimise proofreading errors. .. Fragments generated, either by PCR or cloning in E.coli , were sequenced in both directions using an ABI Prism® 3100 Genetic Analyzer and BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair
    Article Snippet: .. The E. coli uvrD , ssb , and exoI(xonA) genes were amplified by PCR (Supplementary Table ), digested with XbaI , and XhoI (for UvrD ), NdeI and EcoRI (for SSB ) or NdeI and BamHI (for ExoI ), and inserted into pET-29a (Novagen) bacterial expression plasmid. .. Hexa-histidine (his6 ) and Formylglycine Generating Enzyme (FGE) recognition hexa-amino acid sequence (LCTPSR; ald6 ) were introduced onto the C-terminus of EcUvrD and EcExoI proteins.

    Transformation Assay:

    Article Title: Two New Xylanases with Different Substrate Specificities from the Human Gut Bacterium Bacteroides intestinalis DSM 17393
    Article Snippet: .. The purified amplicons were digested using the exonuclease activity of T4 DNA polymerase, annealed with a similarly digested pET-46b vector (EMD Chemicals, Darmstadt, Germany), and introduced into E. coli XL10 Gold competent cells by chemical transformation. .. Individual colonies were picked and cultured overnight in lysogeny broth (LB) supplemented with ampicillin (100 μg/ml), and plasmid DNA was purified using a Plasmid Minikit from Qiagen (Valencia, CA).

    Plasmid Preparation:

    Article Title: MutL sliding clamps coordinate exonuclease-independent Escherichia coli mismatch repair
    Article Snippet: .. The E. coli uvrD , ssb , and exoI(xonA) genes were amplified by PCR (Supplementary Table ), digested with XbaI , and XhoI (for UvrD ), NdeI and EcoRI (for SSB ) or NdeI and BamHI (for ExoI ), and inserted into pET-29a (Novagen) bacterial expression plasmid. .. Hexa-histidine (his6 ) and Formylglycine Generating Enzyme (FGE) recognition hexa-amino acid sequence (LCTPSR; ald6 ) were introduced onto the C-terminus of EcUvrD and EcExoI proteins.

    Article Title: Biochemical and Structural Insights into Xylan Utilization by the Thermophilic Bacterium Caldanaerobius polysaccharolyticus *
    Article Snippet: .. The resultant amplicons were then digested with the exonuclease activity of T4 DNA polymerase and subcloned into pET46 Ek/LIC vector using the Ek/LIC cloning kit (Novagen) and E. coli JM109 as the competent cells by electroporation (Gene Pulser XcellTM from Bio-Rad). .. All recombinant plasmids (pET46- xyl3A , pET46- agu67A , and pET46- xbp1 ) were then extracted using QIAprep spin miniprep kit, and the nucleotides were sequenced (W. M. Keck Center for Comparative and Functional Genomics, University of Illinois) to confirm the integrity of the coding sequence.

    Article Title: Two New Xylanases with Different Substrate Specificities from the Human Gut Bacterium Bacteroides intestinalis DSM 17393
    Article Snippet: .. The purified amplicons were digested using the exonuclease activity of T4 DNA polymerase, annealed with a similarly digested pET-46b vector (EMD Chemicals, Darmstadt, Germany), and introduced into E. coli XL10 Gold competent cells by chemical transformation. .. Individual colonies were picked and cultured overnight in lysogeny broth (LB) supplemented with ampicillin (100 μg/ml), and plasmid DNA was purified using a Plasmid Minikit from Qiagen (Valencia, CA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Millipore exonuclease digestion
    Exonuclease Digestion, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease digestion/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease digestion - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results