exactive plus emr orbitrap lc ms system  (Thermo Fisher)


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    Name:
    Exactive Plus EMR Orbitrap LC MS System
    Description:
    While analysis of denatured proteins may be the most common LC MS protein analysis many biopharmaceutical and biological research applications require analysis of proteins in their native intact state The Thermo Scientific Exactive Plus EMR mass spectrometer combines unsurpassed high resolution accurate mass Thermo Scientific Orbitrap analysis with an extended mass range EMR option to create an outstanding tool for investigating the structure topology and architecture of native like tertiary and quaternary protein structures
    Catalog Number:
    iqlaaegaapfalgmbdl
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Mass Spectrometry
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher exactive plus emr orbitrap lc ms system
    While analysis of denatured proteins may be the most common LC MS protein analysis many biopharmaceutical and biological research applications require analysis of proteins in their native intact state The Thermo Scientific Exactive Plus EMR mass spectrometer combines unsurpassed high resolution accurate mass Thermo Scientific Orbitrap analysis with an extended mass range EMR option to create an outstanding tool for investigating the structure topology and architecture of native like tertiary and quaternary protein structures
    https://www.bioz.com/result/exactive plus emr orbitrap lc ms system/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exactive plus emr orbitrap lc ms system - by Bioz Stars, 2020-07
    96/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology
    Article Snippet: .. Native MS Analysis Identity of aL58ONBY and its decaging efficiency were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher ScientificWaltham, MA, USA) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher ScientificWaltham, MA, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. aL58ONBY was either analyzed in its “as isolated” state or after exposure to UV light (UVP BL-15; Analytik Jena US, Jena, Germany; CA 91786) for 20 min. Next, 100 pmol protein were injected and online buffer was exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column [ ] (P6 polyacrylamide gel, BioRad, Hercules, CA, USA) at a flow-rate of 100 µL per min. Mass spectra were recorded for 1000–8000 m/z at 35,000 resolution as defined at 200 m/z . .. The injection time was set to 200 ms. Voltages applied to the ion optics were optimized to allow for efficient ion transmission while minimizing unintentional ion activa tion.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Design, Synthesis and Evaluation of Oxazaborine Inhibitors of the NLRP3 Inflammasome
    Article Snippet: .. Accurate mass determination was carried out on a Thermo Exactive Plus EMR Orbitrap LC–MS system. ..

    Isolation:

    Article Title: Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology
    Article Snippet: .. Native MS Analysis Identity of aL58ONBY and its decaging efficiency were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher ScientificWaltham, MA, USA) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher ScientificWaltham, MA, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. aL58ONBY was either analyzed in its “as isolated” state or after exposure to UV light (UVP BL-15; Analytik Jena US, Jena, Germany; CA 91786) for 20 min. Next, 100 pmol protein were injected and online buffer was exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column [ ] (P6 polyacrylamide gel, BioRad, Hercules, CA, USA) at a flow-rate of 100 µL per min. Mass spectra were recorded for 1000–8000 m/z at 35,000 resolution as defined at 200 m/z . .. The injection time was set to 200 ms. Voltages applied to the ion optics were optimized to allow for efficient ion transmission while minimizing unintentional ion activa tion.

    Buffer Exchange:

    Article Title: Programmable design of orthogonal protein heterodimers
    Article Snippet: .. Sample purity and integrity were first analyzed using a self-packed buffer exchange column (P6 polyacrylamide gel, BioRad, Hercules CA), coupled online to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific) modified to incorporate a quadrupole mass filter and allow surface-induced dissociation. .. For online buffer-exchange, 200 mM ammonium acetate, pH 6.8 (AmAc) was used as a mobile phase.

    Article Title: Oligomerization Affects the Ability of Human Cyclase-Associated Proteins 1 and 2 to Promote Actin Severing by Cofilins
    Article Snippet: .. Native Mass Spectrometry (Native MS) Sample purity and integrity were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific, Grand Island, NY, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. .. Between 100 and 300 pmole protein (referring to monomer) were injected and online buffer exchanged to 200 mM ammonium acetate, pH 6.8 by a self-packed buffer exchange column [ , ] (P6 polyacrylamide gel; Bio-Rad Laboratories, Hercules, CA, USA) at a flow rate of 100 µL per min. Mass spectra were recorded for 1000–14000 m /z at 8750 resolution, as defined at 200 m /z .

    Article Title: Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology
    Article Snippet: .. Native MS Analysis Identity of aL58ONBY and its decaging efficiency were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher ScientificWaltham, MA, USA) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher ScientificWaltham, MA, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. aL58ONBY was either analyzed in its “as isolated” state or after exposure to UV light (UVP BL-15; Analytik Jena US, Jena, Germany; CA 91786) for 20 min. Next, 100 pmol protein were injected and online buffer was exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column [ ] (P6 polyacrylamide gel, BioRad, Hercules, CA, USA) at a flow-rate of 100 µL per min. Mass spectra were recorded for 1000–8000 m/z at 35,000 resolution as defined at 200 m/z . .. The injection time was set to 200 ms. Voltages applied to the ion optics were optimized to allow for efficient ion transmission while minimizing unintentional ion activa tion.

    Article Title: Controlling protein assembly on inorganic crystals through designed protein interfaces
    Article Snippet: .. Sample purity and integrity was analyzed by on-line buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher Scientific) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation . .. 40 pmole protein (5 μL of 8 μM protein in TBS) were injected and on-line buffer exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column (P6 polyacrylamide gel, BioRad) at a flow-rate of 100 μL per min. Mass spectra were recorded for 1000 – 12000 m/z at 8750 resolution as defined at 200 m/z .

    Mass Spectrometry:

    Article Title: Oligomerization Affects the Ability of Human Cyclase-Associated Proteins 1 and 2 to Promote Actin Severing by Cofilins
    Article Snippet: .. Native Mass Spectrometry (Native MS) Sample purity and integrity were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific, Grand Island, NY, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. .. Between 100 and 300 pmole protein (referring to monomer) were injected and online buffer exchanged to 200 mM ammonium acetate, pH 6.8 by a self-packed buffer exchange column [ , ] (P6 polyacrylamide gel; Bio-Rad Laboratories, Hercules, CA, USA) at a flow rate of 100 µL per min. Mass spectra were recorded for 1000–14000 m /z at 8750 resolution, as defined at 200 m /z .

    Article Title: Architecture of the Complex formed by Large and Small Terminase Subunits from Bacteriophage P22
    Article Snippet: .. MS parameters: backing pressure 10 mbar; capillary 1300 V; cone 60 V; extracted cone 0 V; pressure in the collision cell 2×10-2 mbar; collision energy 30 V. The sample was analyzed on a modified Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific, Bremen) over m/z range 500-20000 [ , ]. ..

    Article Title: Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology
    Article Snippet: .. Native MS Analysis Identity of aL58ONBY and its decaging efficiency were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher ScientificWaltham, MA, USA) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher ScientificWaltham, MA, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. aL58ONBY was either analyzed in its “as isolated” state or after exposure to UV light (UVP BL-15; Analytik Jena US, Jena, Germany; CA 91786) for 20 min. Next, 100 pmol protein were injected and online buffer was exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column [ ] (P6 polyacrylamide gel, BioRad, Hercules, CA, USA) at a flow-rate of 100 µL per min. Mass spectra were recorded for 1000–8000 m/z at 35,000 resolution as defined at 200 m/z . .. The injection time was set to 200 ms. Voltages applied to the ion optics were optimized to allow for efficient ion transmission while minimizing unintentional ion activa tion.

    Article Title: Controlling protein assembly on inorganic crystals through designed protein interfaces
    Article Snippet: .. Sample purity and integrity was analyzed by on-line buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher Scientific) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation . .. 40 pmole protein (5 μL of 8 μM protein in TBS) were injected and on-line buffer exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column (P6 polyacrylamide gel, BioRad) at a flow-rate of 100 μL per min. Mass spectra were recorded for 1000 – 12000 m/z at 8750 resolution as defined at 200 m/z .

    Modification:

    Article Title: Programmable design of orthogonal protein heterodimers
    Article Snippet: .. Mass spectra were subsequently recorded by nanoESI-MS using an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific) modified to incorporate a quadrupole mass filter and allow surface-induced dissociation , , . .. Sample purity and integrity were first analyzed using a self-packed buffer exchange column (P6 polyacrylamide gel, BioRad, Hercules CA), coupled online to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific) modified to incorporate a quadrupole mass filter and allow surface-induced dissociation.

    Article Title: Programmable design of orthogonal protein heterodimers
    Article Snippet: .. Sample purity and integrity were first analyzed using a self-packed buffer exchange column (P6 polyacrylamide gel, BioRad, Hercules CA), coupled online to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific) modified to incorporate a quadrupole mass filter and allow surface-induced dissociation. .. For online buffer-exchange, 200 mM ammonium acetate, pH 6.8 (AmAc) was used as a mobile phase.

    Article Title: Oligomerization Affects the Ability of Human Cyclase-Associated Proteins 1 and 2 to Promote Actin Severing by Cofilins
    Article Snippet: .. Native Mass Spectrometry (Native MS) Sample purity and integrity were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific, Grand Island, NY, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. .. Between 100 and 300 pmole protein (referring to monomer) were injected and online buffer exchanged to 200 mM ammonium acetate, pH 6.8 by a self-packed buffer exchange column [ , ] (P6 polyacrylamide gel; Bio-Rad Laboratories, Hercules, CA, USA) at a flow rate of 100 µL per min. Mass spectra were recorded for 1000–14000 m /z at 8750 resolution, as defined at 200 m /z .

    Article Title: Architecture of the Complex formed by Large and Small Terminase Subunits from Bacteriophage P22
    Article Snippet: .. MS parameters: backing pressure 10 mbar; capillary 1300 V; cone 60 V; extracted cone 0 V; pressure in the collision cell 2×10-2 mbar; collision energy 30 V. The sample was analyzed on a modified Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific, Bremen) over m/z range 500-20000 [ , ]. ..

    Article Title: Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology
    Article Snippet: .. Native MS Analysis Identity of aL58ONBY and its decaging efficiency were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher ScientificWaltham, MA, USA) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher ScientificWaltham, MA, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. aL58ONBY was either analyzed in its “as isolated” state or after exposure to UV light (UVP BL-15; Analytik Jena US, Jena, Germany; CA 91786) for 20 min. Next, 100 pmol protein were injected and online buffer was exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column [ ] (P6 polyacrylamide gel, BioRad, Hercules, CA, USA) at a flow-rate of 100 µL per min. Mass spectra were recorded for 1000–8000 m/z at 35,000 resolution as defined at 200 m/z . .. The injection time was set to 200 ms. Voltages applied to the ion optics were optimized to allow for efficient ion transmission while minimizing unintentional ion activa tion.

    Article Title: Controlling protein assembly on inorganic crystals through designed protein interfaces
    Article Snippet: .. Sample purity and integrity was analyzed by on-line buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher Scientific) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher Scientific) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation . .. 40 pmole protein (5 μL of 8 μM protein in TBS) were injected and on-line buffer exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column (P6 polyacrylamide gel, BioRad) at a flow-rate of 100 μL per min. Mass spectra were recorded for 1000 – 12000 m/z at 8750 resolution as defined at 200 m/z .

    Injection:

    Article Title: Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology
    Article Snippet: .. Native MS Analysis Identity of aL58ONBY and its decaging efficiency were analyzed by online buffer exchange MS using an UltiMate™ 3000 RSLC (Thermo Fisher ScientificWaltham, MA, USA) coupled to an Exactive Plus EMR Orbitrap instrument (Thermo Fisher ScientificWaltham, MA, USA) modified to incorporate a quadrupole mass filter and allow for surface-induced dissociation [ ]. aL58ONBY was either analyzed in its “as isolated” state or after exposure to UV light (UVP BL-15; Analytik Jena US, Jena, Germany; CA 91786) for 20 min. Next, 100 pmol protein were injected and online buffer was exchanged to 200 mM ammonium acetate, pH 6.8 (AmAc) by a self-packed buffer exchange column [ ] (P6 polyacrylamide gel, BioRad, Hercules, CA, USA) at a flow-rate of 100 µL per min. Mass spectra were recorded for 1000–8000 m/z at 35,000 resolution as defined at 200 m/z . .. The injection time was set to 200 ms. Voltages applied to the ion optics were optimized to allow for efficient ion transmission while minimizing unintentional ion activa tion.

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  • Team
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  • 96
    Thermo Fisher exactive plus emr orbitrap lc ms system
    Exactive Plus Emr Orbitrap Lc Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exactive plus emr orbitrap lc ms system/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exactive plus emr orbitrap lc ms system - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

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