exactive orbitrap mass spectrometer  (Thermo Fisher)


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    Name:
    Exactive Plus Orbitrap Mass Spectrometer
    Description:
    Screen identify and quantify compounds in complex samples rapidly and with confidence using the Thermo Scientific Exactive Plus Orbitrap Mass Spectrometer This benchtop LC MS system delivers high resolution accurate mass HR AM data and fast full scan capabilities to increase your sample throughput and confidence in results Easy to use and cost effective to operate it is the ideal instrument for analytical laboratories performing food safety or environmental screening clinical research forensic toxicology DMPK metabolomics and biopharma intact protein analysis applications
    Catalog Number:
    iqlaaegaapfalgmbca
    Price:
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    Applications:
    Industrial & Applied Science|Industrial Mass Spectrometry
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher exactive orbitrap mass spectrometer
    MS spectra (mass range: m/z 200–1000) by <t>Orbitrap</t> and MS/MS spectra (parent ion: m/z 316.1, mass range: 297.0–299.0 and 240.0–242.0) by TSQ for blank paper substrates subject to different treatments. (a) untreated 31 ET paper,
    Screen identify and quantify compounds in complex samples rapidly and with confidence using the Thermo Scientific Exactive Plus Orbitrap Mass Spectrometer This benchtop LC MS system delivers high resolution accurate mass HR AM data and fast full scan capabilities to increase your sample throughput and confidence in results Easy to use and cost effective to operate it is the ideal instrument for analytical laboratories performing food safety or environmental screening clinical research forensic toxicology DMPK metabolomics and biopharma intact protein analysis applications
    https://www.bioz.com/result/exactive orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    exactive orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Quantitative Paper Spray Mass Spectrometry Analysis of Drugs of Abuse"

    Article Title: Quantitative Paper Spray Mass Spectrometry Analysis of Drugs of Abuse

    Journal: The Analyst

    doi: 10.1039/c3an00934c

    MS spectra (mass range: m/z 200–1000) by Orbitrap and MS/MS spectra (parent ion: m/z 316.1, mass range: 297.0–299.0 and 240.0–242.0) by TSQ for blank paper substrates subject to different treatments. (a) untreated 31 ET paper,
    Figure Legend Snippet: MS spectra (mass range: m/z 200–1000) by Orbitrap and MS/MS spectra (parent ion: m/z 316.1, mass range: 297.0–299.0 and 240.0–242.0) by TSQ for blank paper substrates subject to different treatments. (a) untreated 31 ET paper,

    Techniques Used: Mass Spectrometry

    2) Product Images from "Analysis of high-molecular-weight fructan polymers in crude plant extracts by high-resolution LC-MS"

    Article Title: Analysis of high-molecular-weight fructan polymers in crude plant extracts by high-resolution LC-MS

    Journal: Analytical and Bioanalytical Chemistry

    doi: 10.1007/s00216-011-5374-8

    Extracted ion chromatograms for DP = 3, 4, 5, 6, 7, 8, 10, 20, 35, 62,71 and 75 showing the peaks obtained by the analysis of extracts of the same sample using either an ion trap ( left ) at unit resolution or the Exactive Orbitrap ( right ) at 100 K resolution. The traces are similar in the lower DP fructans, as would be expected. However, the larger DP fructans were not detectable using the ion trap
    Figure Legend Snippet: Extracted ion chromatograms for DP = 3, 4, 5, 6, 7, 8, 10, 20, 35, 62,71 and 75 showing the peaks obtained by the analysis of extracts of the same sample using either an ion trap ( left ) at unit resolution or the Exactive Orbitrap ( right ) at 100 K resolution. The traces are similar in the lower DP fructans, as would be expected. However, the larger DP fructans were not detectable using the ion trap

    Techniques Used:

    Distribution of high-DP fructans in blade extracts from L. perenne cultivars with contrasting total sugar content, with measured areas displayed on a log10 scale. Extracts were chromatographically separated and analysed by electrospray ionisation in negative mode with an Orbitrap MS. Dark grey bars depict a standard (Fennema) and light grey bars a high-sugar cultivar (Expo). Significantly higher levels of high-DP fructans are present in Expo than in Fennema
    Figure Legend Snippet: Distribution of high-DP fructans in blade extracts from L. perenne cultivars with contrasting total sugar content, with measured areas displayed on a log10 scale. Extracts were chromatographically separated and analysed by electrospray ionisation in negative mode with an Orbitrap MS. Dark grey bars depict a standard (Fennema) and light grey bars a high-sugar cultivar (Expo). Significantly higher levels of high-DP fructans are present in Expo than in Fennema

    Techniques Used: Mass Spectrometry

    Charge state distribution vs. degree of polymerisation of fructans in ryegrass extracts analysed by electrospray ionisation in negative mode in an Orbitrap MS: singly charged ( dark diamonds ), doubly charged ( light squares ), triply charged ( mid-grey triangles ), quadruply charged ( dark circles ), five charges ( light stars )
    Figure Legend Snippet: Charge state distribution vs. degree of polymerisation of fructans in ryegrass extracts analysed by electrospray ionisation in negative mode in an Orbitrap MS: singly charged ( dark diamonds ), doubly charged ( light squares ), triply charged ( mid-grey triangles ), quadruply charged ( dark circles ), five charges ( light stars )

    Techniques Used: Mass Spectrometry

    3) Product Images from "Liquid chromatography - high resolution mass spectrometry analysis of fatty acid metabolism"

    Article Title: Liquid chromatography - high resolution mass spectrometry analysis of fatty acid metabolism

    Journal: Analytical chemistry

    doi: 10.1021/ac202220b

    (A) Chromatographic traces of saturated fatty acid standards of varying carbon chain lengths, and (B) of fatty acids with the same carbon chain length but differing degrees of unsaturation. Liquid chromatographic (LC) separation was performed using a C 8 reversed-phase column and a water/methanol gradient containing tributylamine as an ion-pairing agent (see text for details). The LC eluent was ionized with electrospray ionization (negative mode) and subsequently analyzed by a stand-alone orbitrap mass spectrometer.
    Figure Legend Snippet: (A) Chromatographic traces of saturated fatty acid standards of varying carbon chain lengths, and (B) of fatty acids with the same carbon chain length but differing degrees of unsaturation. Liquid chromatographic (LC) separation was performed using a C 8 reversed-phase column and a water/methanol gradient containing tributylamine as an ion-pairing agent (see text for details). The LC eluent was ionized with electrospray ionization (negative mode) and subsequently analyzed by a stand-alone orbitrap mass spectrometer.

    Techniques Used: Mass Spectrometry

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans
    Article Snippet: .. For identification of selenium metabolites the HPLC system was coupled to a Q-Exactive Orbitrap Mass Spectrometer (Thermo Scientific, Waltham, USA) equipped with a heated electrospray ionization (HESI2) source from the same manufacturer. .. MS was performed in the positive mode and source settings were: gas temp 400°C, flow rates 47 (sheath) and 11 (aux) instrument units, spray voltage 3500 V and capillary temp 250°C.

    Liquid Chromatography:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Mass Spectrometry:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Article Title: Serial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland
    Article Snippet: .. Lipids were analyzed using a high resolution hybrid QExactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific) in DDA mode (Top 8) using positive/negative ion polarity switching. ..

    Article Title: Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans
    Article Snippet: .. For identification of selenium metabolites the HPLC system was coupled to a Q-Exactive Orbitrap Mass Spectrometer (Thermo Scientific, Waltham, USA) equipped with a heated electrospray ionization (HESI2) source from the same manufacturer. .. MS was performed in the positive mode and source settings were: gas temp 400°C, flow rates 47 (sheath) and 11 (aux) instrument units, spray voltage 3500 V and capillary temp 250°C.

    Article Title: Quantitative Paper Spray Mass Spectrometry Analysis of Drugs of Abuse
    Article Snippet: .. The MS spectra were recorded using an Exactive Orbitrap mass spectrometer (Exactive, Thermo Scientific, CA). .. Quantitation of drugs in blood was carried out by tandem mass spectrometry (MS/MS) using a TSQ Quantum Access Max mass spectrometer (Thermo Scientific, CA) in the multiple reaction monitoring (MRM) mode with an isolation widow width of m/z 0.4.

    Article Title: Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.)
    Article Snippet: .. Tryptic digestions of Pa PPO were analyzed by nanoUHPLC–ESI–MS/MS using a high-resolution Orbitrap mass spectrometer (Dionex Ultimate 3000 RSLCnano, Q Exactive Orbitrap, Thermo Scientific). .. The data analysis was performed with Proteome Discoverer 1.4 by searching against the P. armeniaca entries from the UniProt database (containing all listed proteins from P. mume , P. persica , and P. armeniaca ).

    Article Title: The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes
    Article Snippet: .. In 2012, Heck et al . first demonstrated the use of an Orbitrap mass spectrometer (Exactive Plus™, Thermo Fisher Scientific) for the analysis of intact protein complexes ranging from 150 kDa to 800 kDa. .. – They subsequently extended this range to include virus-like nanoparticles with a mass up to 4.5 MDa ( ).

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

    Article Title: Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery
    Article Snippet: .. These fractions were desalted using C18 OMIX® tips (Agilent) and analysed on a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) coupled to an EASY-nLC1000 (Thermo Scientific). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Tandem Mass Spectroscopy:

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

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