ex527 (49843-98-3)  (Millipore)

Journal: Nature Communications

Article Title: Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43

doi: 10.1038/s41467-022-28822-7

Figure Lengend Snippet: a HEK293E sh TDP-43 cells were cotransfected with E451, K136 TAG TDP-43 and SIRT1 or SIRT2. Twenty-four hours before lysis, 5 mM acK and DMSO, 1 µM or 10 µM Ex527 containing media was supplied to the cells. N = 3 biological replicates. Source data are provided as a Source Data file. b Stably amber suppressed sh TDP-43 -HEK293E cells were cotransfected with different sirtuin constructs together with K136 TAG TDP-43. Cells were fixed after 24 h in the presence of 5 mM acK. Scale bar represents 10 µm. c Quantification of the number of cells with TDP-43 positive inclusions (6xHis staining). A total of 200 cells from 4 independent experiments were recorded per condition. Unpaired, two-sided t -test was used for comparison. Data are presented as mean values ± SEM. ** p = 0.0083. Source data are provided as a Source Data file.

Article Snippet: When mentioned, Ex527 (CAS 49843-98-3, Santa Cruz Biotechnology) was dissolved in DMSO and added to the cultured cells 24 h before lysis to a final concentration of 1 or 10 μM.

Techniques: Lysis, Stable Transfection, Construct, Staining, Comparison