ex taq polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ex taq polymerase
    Ex Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ex taq polymerase/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ex taq polymerase - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase
    Article Snippet: .. Six histidine codons were introduced onto the N-terminus of the Taq DNA polymerase and Taq DNA pol/TBD gene and the recombinant genes were cloned in frame into the NdeI site of the pLEX vector (Invitrogen). .. 5′–3′ Exonuclease-deficient mutants which lack the first 235 amino acids of the N-terminal were likewise generated.

    Amplification:

    Article Title: Epigenetic Reactivation of RASSF1A by Phenethyl Isothiocyanate (PEITC) and promotion of apoptosis in LNCaP cells
    Article Snippet: .. Briefly, the DNA sequences were amplified by mixing bisulfite-converted DNA (500 ng) with primer MU379 (50 pmoles) and primer ML730 (50 pmoles) in reaction buffer (20 μl) containing dNTPs (200 μM each dNTP) using Platinum PCR Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA). .. The reaction conditions consisted of 30 cycles of 95 °C for 1 min, 55 °C for 1 min and 74 °C for 2 min. A semi-nested PCR using the amplified products at a 1:50 dilution, the internal primer ML561 and primer MU379 was performed using similar PCR conditions as described above.

    Article Title: A test of somatic mosaicism in the androgen receptor gene of Canada lynx (Lynx canadensis)
    Article Snippet: .. Amplification was conducted in a 10ul reaction containing deionized water (Invitrogen), 10X PCR Reaction Buffer (Invitrogen), 50 mM MgCl2 (Invitrogen), 100 mM dNTP solution (Invitrogen), 3 mg/mL BSA, 40uM forward and reverse primers (Integrated DNA Technologies) mentioned above (forward primers labeled with the fluorescent dye HEX), 0.0375U Invitrogen Taq DNA Polymerase, and 5 ng of DNA. .. The PCR reaction was run in a Bio-Rad DNA Engine Dyad and Dyad Disciple thermocycler under the following conditions: 94 °C for 15 min; followed by 29 cycles of 94 °C for 30 s, 52 °C for 1 min 30 s, and 72 °C for 1 min 30 s, and completed with a step of 60 °C for 45 min. Amplified samples were run on an 80 mL, 1.5 % agarose gel stained with ethidium bromide at 90 volts for 45 min, and visualized under ultraviolet light and to determine sex.

    Labeling:

    Article Title: A test of somatic mosaicism in the androgen receptor gene of Canada lynx (Lynx canadensis)
    Article Snippet: .. Amplification was conducted in a 10ul reaction containing deionized water (Invitrogen), 10X PCR Reaction Buffer (Invitrogen), 50 mM MgCl2 (Invitrogen), 100 mM dNTP solution (Invitrogen), 3 mg/mL BSA, 40uM forward and reverse primers (Integrated DNA Technologies) mentioned above (forward primers labeled with the fluorescent dye HEX), 0.0375U Invitrogen Taq DNA Polymerase, and 5 ng of DNA. .. The PCR reaction was run in a Bio-Rad DNA Engine Dyad and Dyad Disciple thermocycler under the following conditions: 94 °C for 15 min; followed by 29 cycles of 94 °C for 30 s, 52 °C for 1 min 30 s, and 72 °C for 1 min 30 s, and completed with a step of 60 °C for 45 min. Amplified samples were run on an 80 mL, 1.5 % agarose gel stained with ethidium bromide at 90 volts for 45 min, and visualized under ultraviolet light and to determine sex.

    Polymerase Chain Reaction:

    Article Title: Epigenetic Reactivation of RASSF1A by Phenethyl Isothiocyanate (PEITC) and promotion of apoptosis in LNCaP cells
    Article Snippet: .. Briefly, the DNA sequences were amplified by mixing bisulfite-converted DNA (500 ng) with primer MU379 (50 pmoles) and primer ML730 (50 pmoles) in reaction buffer (20 μl) containing dNTPs (200 μM each dNTP) using Platinum PCR Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA). .. The reaction conditions consisted of 30 cycles of 95 °C for 1 min, 55 °C for 1 min and 74 °C for 2 min. A semi-nested PCR using the amplified products at a 1:50 dilution, the internal primer ML561 and primer MU379 was performed using similar PCR conditions as described above.

    Article Title: Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish
    Article Snippet: .. All PCR reactions were performed using either NEB 2X Taq Master Mix (M0270L), Thermo Scientific Taq (EP0402 and 2x PCR Master Mix Cat# AB-0575/DC), or Kapa 2G Fast ReadyMix +dye (Kapa Biosystems-KM5101). .. CRISPR activity was assessed by either direct sequencing of PCR amplicons, T7 assay (NEB M0302S), Surveyor assay (IDT 706025), or RFLP.

    Article Title: A test of somatic mosaicism in the androgen receptor gene of Canada lynx (Lynx canadensis)
    Article Snippet: .. Amplification was conducted in a 10ul reaction containing deionized water (Invitrogen), 10X PCR Reaction Buffer (Invitrogen), 50 mM MgCl2 (Invitrogen), 100 mM dNTP solution (Invitrogen), 3 mg/mL BSA, 40uM forward and reverse primers (Integrated DNA Technologies) mentioned above (forward primers labeled with the fluorescent dye HEX), 0.0375U Invitrogen Taq DNA Polymerase, and 5 ng of DNA. .. The PCR reaction was run in a Bio-Rad DNA Engine Dyad and Dyad Disciple thermocycler under the following conditions: 94 °C for 15 min; followed by 29 cycles of 94 °C for 30 s, 52 °C for 1 min 30 s, and 72 °C for 1 min 30 s, and completed with a step of 60 °C for 45 min. Amplified samples were run on an 80 mL, 1.5 % agarose gel stained with ethidium bromide at 90 volts for 45 min, and visualized under ultraviolet light and to determine sex.

    other:

    Article Title: Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization
    Article Snippet: The processivity values determined with the use of a heparin trap were much lower than the values previously published for the Taq DNA polymerase [ , ] and were within a range of 80 nt to 160 nt.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: An Endogenous Murine Leukemia Viral Genome Contaminant in a Commercial RT-PCR Kit is Amplified Using Standard Primers for XMRV
    Article Snippet: .. In this study, we evaluated several one-step RT-PCR kits and a Taq DNA polymerase for the contamination of MLV-related genomes and found that the test kit and the Taq DNA polymerase from Invitrogen were contaminated with MLV-related genomes. .. The findings in the present study indicate that contaminating nucleic acids in the test kits can potentially produce false-positive PCR results in studies of XMRV and other MLV-related viruses.

    Recombinant:

    Article Title: Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase
    Article Snippet: .. Six histidine codons were introduced onto the N-terminus of the Taq DNA polymerase and Taq DNA pol/TBD gene and the recombinant genes were cloned in frame into the NdeI site of the pLEX vector (Invitrogen). .. 5′–3′ Exonuclease-deficient mutants which lack the first 235 amino acids of the N-terminal were likewise generated.

    Plasmid Preparation:

    Article Title: Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase
    Article Snippet: .. Six histidine codons were introduced onto the N-terminus of the Taq DNA polymerase and Taq DNA pol/TBD gene and the recombinant genes were cloned in frame into the NdeI site of the pLEX vector (Invitrogen). .. 5′–3′ Exonuclease-deficient mutants which lack the first 235 amino acids of the N-terminal were likewise generated.

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    Thermo Fisher sybr premix ex taq kit
    Sybr Premix Ex Taq Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr premix ex taq ii
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    Thermo Fisher sybr green pcr premix ex taq ii reagents
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