ex taq polymerase  (Millipore)


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    Structured Review

    Millipore ex taq polymerase
    Ex Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ex taq polymerase/product/Millipore
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ex taq polymerase - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication
    Article Snippet: The forward and reverse primers for the amplification of λ xis gene fragment were synthesized by Sigma-Aldrich and their sequences were 5'-CCTGCTCTGCCGCTTCACGC-3' and 5'-TCCGGATAAAAACGTCGATGACATTTGC-3' , respectively. .. The volume of the reaction mixture was 25 μL, which was composed of 2.5 μL of 10× standard Taq reaction buffer, 0.5 μL of 1 mM deoxynucleotide solution, 0.5 μL of each of the primers (10 μM), 0.125 μL of Taq DNA polymerase; selected volume of water or drugs diluted with water (sterile, ACS purity, Sigma-Aldrich) and 0.5 μL of bacteriophage λ DNA.

    Article Title: Deficiency of AtGFAT1 activity impairs growth, pollen germination and tolerance to tunicamycin in Arabidopsis
    Article Snippet: A 1 µl aliquot of the reverse transcribed reaction was used as a template for PCR with RED Taq DNA polymerase (Sigma-Aldrich, St Louis, MO, USA). .. PCR cycling conditions were as follows: 94 °C for 1 min, followed by 25 cycles at 94 °C for 1 min, 62 °C for 30 s, and 72 °C for 30 s, and a final elongation step at 72 °C for 15 min. Amplified products were visualized on a 1.2% (w/v) agarose gel.

    Article Title: t-plasminogen activator inhibitor-1 polymorphism in idiopathic pulmonary arterial hypertension
    Article Snippet: A 12.5-µL PCR mixture was prepared containing 100 ng of genomic DNA, 0.2 mM of both forward and reverse primers, 10 mM dNTP's, 10XPCR buffer [10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2 mM MgCl2 ], 2.5% of formamide, 2U of Taq DNA polymerase (Sigma Aldrich, Germany). .. [ ] The PCR conditions optimized for the amplification of PAI-1 were for 40 cycles with denaturation at 94°C for 30 s; annealing temperature at 60°C for 30 s; and elongation at 72°C for 1 min with initial denaturation of 95°C for 5 min and final extension of 72°C for 5 min. PCR-amplified products were digested by adding 5 U of 1X Hind III restriction enzyme (Bangalore Genei, India) with an overnight incubation at 37°C.

    Article Title: Biochemical and Molecular Chitotriosidase Profiles in Patients with Gaucher Disease Type 1 in Minas Gerais, Brazil: New Mutation in CHIT1 Gene
    Article Snippet: PCR amplification of the regions containing the polymorphisms G102S (exon 4), G354R (exon 11), and A442V (exon 13) was performed using specific primers detailed in Table . .. The 40 μL reaction contained 1x PCR Buffer (Sigma-Aldrich Co., St. Louis, MO, USA), 1.5 mM dNTPs, 0.2 mM of each primer, 2.5 mM MgCl2 , 1 U Taq polymerase (Sigma-Aldrich Co., St. Louis, MO, USA), and 100 ng of DNA template.

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: .. The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The following PCR reaction conditions were used: 4 min at 94°C for denaturation, 30 cycles each of 30 sec at 94°C for denaturation, 1min at 58.2°C for annealing, 2 min at 72°C for extension followed by the final extension at 72°C for 7 min.

    Article Title: p53 is necessary for the adaptive changes in cellular milieu subsequent to an acute bout of endurance exercise
    Article Snippet: Extracted DNA was added to a PCR tube containing DNA Taq polymerase (JumpStart REDtaq ReadyMix PCR reaction mix; Sigma-Aldrich, St. Louis, MO) and forward and reverse primers for the WT or the mutated p53 gene. .. Differences in the genome were detected using PCR amplification.

    Article Title: In-Vitro Helix Opening of M. tuberculosis oriC by DnaA Occurs at Precise Location and Is Inhibited by IciA Like Protein
    Article Snippet: Primer extension 10 μl of the primer extension mix included 200 μM each dNTPs, 0.04 pM 32 P end labeled primer [SeqOriR1, SeqOriR2 or SeqOriR3 ( )] 0.5 mM MgCl2 , 2% DMSO and 0.5 Units Taq DNA polymerase (SIGMA). .. The mixture was subjected to primer extension (SeqOriR1) in a thermocycler for 30 cycles: 94°C for 1 min, 92°C for 30 sec., 54°C for 30 sec. and 72°C for 1 min except for 5 min in the last amplification cycle.

    Synthesized:

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication
    Article Snippet: The forward and reverse primers for the amplification of λ xis gene fragment were synthesized by Sigma-Aldrich and their sequences were 5'-CCTGCTCTGCCGCTTCACGC-3' and 5'-TCCGGATAAAAACGTCGATGACATTTGC-3' , respectively. .. The volume of the reaction mixture was 25 μL, which was composed of 2.5 μL of 10× standard Taq reaction buffer, 0.5 μL of 1 mM deoxynucleotide solution, 0.5 μL of each of the primers (10 μM), 0.125 μL of Taq DNA polymerase; selected volume of water or drugs diluted with water (sterile, ACS purity, Sigma-Aldrich) and 0.5 μL of bacteriophage λ DNA.

    Construct:

    Article Title: A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    Article Snippet: The Taq DNA polymerase–antibody complex was prepared with ‘Jumpstart’ Taq DNA polymerase antibody (Sigma-Aldrich, Missouri, USA) according to instructions. .. Standards to estimate cell number equivalence of gDNA were also constructed.

    SYBR Green Assay:

    Article Title: A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    Article Snippet: Twenty-five microlitres of qLM–PCR reactions contained 7.5 µl diluted annealing/ligation reaction, 0.63 µl additional 50 pmol/µl 24-mer, and final concentrations of 1× Taq DNA polymerase buffer (Fisher Biotech, Wembley, Western Australia), 320 μM (each) dATP, dTTP, dGTP, dCTP (Promega), 2 mM MgCl2 , 0.4× SYBR Green I (Invitrogen), PCR grade water and 0.1 U/μl Taq DNA polymerase (Fisher Biotech or Scientifix, Cheltenham, Victoria, Australia) as a Taq DNA polymerase–antibody complex allowing a PCR hot-start. .. The Taq DNA polymerase–antibody complex was prepared with ‘Jumpstart’ Taq DNA polymerase antibody (Sigma-Aldrich, Missouri, USA) according to instructions.

    Incubation:

    Article Title: t-plasminogen activator inhibitor-1 polymorphism in idiopathic pulmonary arterial hypertension
    Article Snippet: A 12.5-µL PCR mixture was prepared containing 100 ng of genomic DNA, 0.2 mM of both forward and reverse primers, 10 mM dNTP's, 10XPCR buffer [10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2 mM MgCl2 ], 2.5% of formamide, 2U of Taq DNA polymerase (Sigma Aldrich, Germany). .. [ ] The PCR conditions optimized for the amplification of PAI-1 were for 40 cycles with denaturation at 94°C for 30 s; annealing temperature at 60°C for 30 s; and elongation at 72°C for 1 min with initial denaturation of 95°C for 5 min and final extension of 72°C for 5 min. PCR-amplified products were digested by adding 5 U of 1X Hind III restriction enzyme (Bangalore Genei, India) with an overnight incubation at 37°C.

    Activity Assay:

    Article Title: Biochemical and Molecular Chitotriosidase Profiles in Patients with Gaucher Disease Type 1 in Minas Gerais, Brazil: New Mutation in CHIT1 Gene
    Article Snippet: The three patients (P1, P2, and P3) who presented null or low levels of ChT activity in spite of being homozygous or heterozygous for the dup24 wild type allele were screened for three polymorphisms in CHIT1 that correlate with reduced ChT activity: G102S, G354R, and A442V (Bussink et al. ; Grace et al. ; Lee et al. ). .. The 40 μL reaction contained 1x PCR Buffer (Sigma-Aldrich Co., St. Louis, MO, USA), 1.5 mM dNTPs, 0.2 mM of each primer, 2.5 mM MgCl2 , 1 U Taq polymerase (Sigma-Aldrich Co., St. Louis, MO, USA), and 100 ng of DNA template.

    Western Blot:

    Article Title: A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    Article Snippet: Twenty-five microlitres of qLM–PCR reactions contained 7.5 µl diluted annealing/ligation reaction, 0.63 µl additional 50 pmol/µl 24-mer, and final concentrations of 1× Taq DNA polymerase buffer (Fisher Biotech, Wembley, Western Australia), 320 μM (each) dATP, dTTP, dGTP, dCTP (Promega), 2 mM MgCl2 , 0.4× SYBR Green I (Invitrogen), PCR grade water and 0.1 U/μl Taq DNA polymerase (Fisher Biotech or Scientifix, Cheltenham, Victoria, Australia) as a Taq DNA polymerase–antibody complex allowing a PCR hot-start. .. The Taq DNA polymerase–antibody complex was prepared with ‘Jumpstart’ Taq DNA polymerase antibody (Sigma-Aldrich, Missouri, USA) according to instructions.

    Chromatography:

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Ligation:

    Article Title: A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    Article Snippet: At the 10 min point of 10°C the program was paused and 2.4 U T4 DNA ligase (Invitrogen, diluted from 5 U/µl to 1 U/μl in PCR grade water) was added, mixed and the temperature continued for 10°C/10 min then ramped to 16°C/16 h for ligation. .. The Taq DNA polymerase–antibody complex was prepared with ‘Jumpstart’ Taq DNA polymerase antibody (Sigma-Aldrich, Missouri, USA) according to instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Deficiency of AtGFAT1 activity impairs growth, pollen germination and tolerance to tunicamycin in Arabidopsis
    Article Snippet: Paragraph title: RNA isolation, RT-PCR and quantitative real-time PCR ... A 1 µl aliquot of the reverse transcribed reaction was used as a template for PCR with RED Taq DNA polymerase (Sigma-Aldrich, St Louis, MO, USA).

    Generated:

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Sequencing:

    Article Title: A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    Article Snippet: The Taq DNA polymerase–antibody complex was prepared with ‘Jumpstart’ Taq DNA polymerase antibody (Sigma-Aldrich, Missouri, USA) according to instructions. .. Cycling conditions were as follows: Employing a Stratagene MX3000P instrument (Agilent Technologies, Stratagene, La Jolla, CA, USA) with software MXPro version 4.10, qLM–PCR reactions were heated to 94°C for 1 min to activate Taq DNA polymerase and release 12-mers, then ramped to 72°C for 4 min to re-anneal the target DNA and generate complementary sequence to the ligated 24-mers.

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: Paragraph title: DNA extraction, amplification of ITS region and sequencing: ... The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich).

    Article Title: CACNA1H Mutations Are Associated With Different Forms of Primary Aldosteronism
    Article Snippet: Paragraph title: Sanger Sequencing ... PCR was performed on 100 ng of DNA in a final volume of 25 μL containing 0.75 mM MgCl2 , 400 nM of each primer, 200 μM deoxynucleotide triphosphate, and 1.25 U Taq DNA Polymerase (Sigma).

    Article Title: In-Vitro Helix Opening of M. tuberculosis oriC by DnaA Occurs at Precise Location and Is Inhibited by IciA Like Protein
    Article Snippet: Primer extension 10 μl of the primer extension mix included 200 μM each dNTPs, 0.04 pM 32 P end labeled primer [SeqOriR1, SeqOriR2 or SeqOriR3 ( )] 0.5 mM MgCl2 , 2% DMSO and 0.5 Units Taq DNA polymerase (SIGMA). .. The reactions were stopped by adding 2 μl of formamide sequencing dye (95% Formamide, 10 mM NaOH, 0.05% Bromophenol blue and 0.05%Xylene Cyanol FF).

    DNA Extraction:

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: Paragraph title: DNA extraction, amplification of ITS region and sequencing: ... The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich).

    Fluorescence:

    Article Title: A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    Article Snippet: The Taq DNA polymerase–antibody complex was prepared with ‘Jumpstart’ Taq DNA polymerase antibody (Sigma-Aldrich, Missouri, USA) according to instructions. .. PCR then proceeded over 40 cycles of 94°C for 1 min followed by annealing/extension at 72°C for 3 min. Fluorescence data was collected at the end of annealing/extension steps.

    Mutagenesis:

    Article Title: Biochemical and Molecular Chitotriosidase Profiles in Patients with Gaucher Disease Type 1 in Minas Gerais, Brazil: New Mutation in CHIT1 Gene
    Article Snippet: Genotypes were determined by the presence or absence of wild type (75 bp) and/or mutant (99 bp) bands. .. The 40 μL reaction contained 1x PCR Buffer (Sigma-Aldrich Co., St. Louis, MO, USA), 1.5 mM dNTPs, 0.2 mM of each primer, 2.5 mM MgCl2 , 1 U Taq polymerase (Sigma-Aldrich Co., St. Louis, MO, USA), and 100 ng of DNA template.

    Isolation:

    Article Title: Deficiency of AtGFAT1 activity impairs growth, pollen germination and tolerance to tunicamycin in Arabidopsis
    Article Snippet: Paragraph title: RNA isolation, RT-PCR and quantitative real-time PCR ... A 1 µl aliquot of the reverse transcribed reaction was used as a template for PCR with RED Taq DNA polymerase (Sigma-Aldrich, St Louis, MO, USA).

    Size-exclusion Chromatography:

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The following PCR reaction conditions were used: 4 min at 94°C for denaturation, 30 cycles each of 30 sec at 94°C for denaturation, 1min at 58.2°C for annealing, 2 min at 72°C for extension followed by the final extension at 72°C for 7 min.

    Article Title: In-Vitro Helix Opening of M. tuberculosis oriC by DnaA Occurs at Precise Location and Is Inhibited by IciA Like Protein
    Article Snippet: Primer extension 10 μl of the primer extension mix included 200 μM each dNTPs, 0.04 pM 32 P end labeled primer [SeqOriR1, SeqOriR2 or SeqOriR3 ( )] 0.5 mM MgCl2 , 2% DMSO and 0.5 Units Taq DNA polymerase (SIGMA). .. The mixture was subjected to primer extension (SeqOriR1) in a thermocycler for 30 cycles: 94°C for 1 min, 92°C for 30 sec., 54°C for 30 sec. and 72°C for 1 min except for 5 min in the last amplification cycle.

    Labeling:

    Article Title: In-Vitro Helix Opening of M. tuberculosis oriC by DnaA Occurs at Precise Location and Is Inhibited by IciA Like Protein
    Article Snippet: .. Primer extension 10 μl of the primer extension mix included 200 μM each dNTPs, 0.04 pM 32 P end labeled primer [SeqOriR1, SeqOriR2 or SeqOriR3 ( )] 0.5 mM MgCl2 , 2% DMSO and 0.5 Units Taq DNA polymerase (SIGMA). .. The mixture was subjected to primer extension (SeqOriR1) in a thermocycler for 30 cycles: 94°C for 1 min, 92°C for 30 sec., 54°C for 30 sec. and 72°C for 1 min except for 5 min in the last amplification cycle.

    Mouse Assay:

    Article Title: p53 is necessary for the adaptive changes in cellular milieu subsequent to an acute bout of endurance exercise
    Article Snippet: Heterozygous p53 mice were bred to produce homozygous p53 knockout (KO) and littermate wild-type (WT) mice and were treated experimentally, as outlined in protocols approved by the York Animal Care Committee in accordance with the Canadian Council on Animal Care. .. Extracted DNA was added to a PCR tube containing DNA Taq polymerase (JumpStart REDtaq ReadyMix PCR reaction mix; Sigma-Aldrich, St. Louis, MO) and forward and reverse primers for the WT or the mutated p53 gene.

    Polymerase Chain Reaction:

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication
    Article Snippet: Paragraph title: Polymerase chain reaction (PCR) ... The volume of the reaction mixture was 25 μL, which was composed of 2.5 μL of 10× standard Taq reaction buffer, 0.5 μL of 1 mM deoxynucleotide solution, 0.5 μL of each of the primers (10 μM), 0.125 μL of Taq DNA polymerase; selected volume of water or drugs diluted with water (sterile, ACS purity, Sigma-Aldrich) and 0.5 μL of bacteriophage λ DNA.

    Article Title: Deficiency of AtGFAT1 activity impairs growth, pollen germination and tolerance to tunicamycin in Arabidopsis
    Article Snippet: .. A 1 µl aliquot of the reverse transcribed reaction was used as a template for PCR with RED Taq DNA polymerase (Sigma-Aldrich, St Louis, MO, USA). .. Each cDNA sample was diluted 10 times and used for RT-PCR using specific primer pairs listed in .

    Article Title: A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    Article Snippet: Twenty-five microlitres of qLM–PCR reactions contained 7.5 µl diluted annealing/ligation reaction, 0.63 µl additional 50 pmol/µl 24-mer, and final concentrations of 1× Taq DNA polymerase buffer (Fisher Biotech, Wembley, Western Australia), 320 μM (each) dATP, dTTP, dGTP, dCTP (Promega), 2 mM MgCl2 , 0.4× SYBR Green I (Invitrogen), PCR grade water and 0.1 U/μl Taq DNA polymerase (Fisher Biotech or Scientifix, Cheltenham, Victoria, Australia) as a Taq DNA polymerase–antibody complex allowing a PCR hot-start. .. The Taq DNA polymerase–antibody complex was prepared with ‘Jumpstart’ Taq DNA polymerase antibody (Sigma-Aldrich, Missouri, USA) according to instructions.

    Article Title: t-plasminogen activator inhibitor-1 polymorphism in idiopathic pulmonary arterial hypertension
    Article Snippet: .. A 12.5-µL PCR mixture was prepared containing 100 ng of genomic DNA, 0.2 mM of both forward and reverse primers, 10 mM dNTP's, 10XPCR buffer [10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2 mM MgCl2 ], 2.5% of formamide, 2U of Taq DNA polymerase (Sigma Aldrich, Germany). ..

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins
    Article Snippet: Paragraph title: Optimization of PCR conditions for successful gene synthesis protocol ... Four DNA polymerases were selected for these studies: KOD Hot Start DNA polymerase (EMD-Millipore), Q5® Hot Star High Fidelity DNA polymerase (New England Biolabs), Pfu Turbo DNA polymerase (Agilent Technologies) and Taq DNA polymerase (Sigma-Aldrich).

    Article Title: Biochemical and Molecular Chitotriosidase Profiles in Patients with Gaucher Disease Type 1 in Minas Gerais, Brazil: New Mutation in CHIT1 Gene
    Article Snippet: .. The 40 μL reaction contained 1x PCR Buffer (Sigma-Aldrich Co., St. Louis, MO, USA), 1.5 mM dNTPs, 0.2 mM of each primer, 2.5 mM MgCl2 , 1 U Taq polymerase (Sigma-Aldrich Co., St. Louis, MO, USA), and 100 ng of DNA template. .. The DNA was denatured at 95 °C for 2 min, and amplification was performed by 35 cycles at 95 °C for 20 s, 56 °C for 20 s, and 72 °C for 30 s. The amplified fragments were separated by electrophoresis on a 2% agarose gel and visualized by ethidium bromide staining.

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: .. The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The following PCR reaction conditions were used: 4 min at 94°C for denaturation, 30 cycles each of 30 sec at 94°C for denaturation, 1min at 58.2°C for annealing, 2 min at 72°C for extension followed by the final extension at 72°C for 7 min.

    Article Title: CACNA1H Mutations Are Associated With Different Forms of Primary Aldosteronism
    Article Snippet: .. PCR was performed on 100 ng of DNA in a final volume of 25 μL containing 0.75 mM MgCl2 , 400 nM of each primer, 200 μM deoxynucleotide triphosphate, and 1.25 U Taq DNA Polymerase (Sigma). ..

    Article Title: p53 is necessary for the adaptive changes in cellular milieu subsequent to an acute bout of endurance exercise
    Article Snippet: .. Extracted DNA was added to a PCR tube containing DNA Taq polymerase (JumpStart REDtaq ReadyMix PCR reaction mix; Sigma-Aldrich, St. Louis, MO) and forward and reverse primers for the WT or the mutated p53 gene. .. Differences in the genome were detected using PCR amplification.

    Purification:

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication
    Article Snippet: The volume of the reaction mixture was 25 μL, which was composed of 2.5 μL of 10× standard Taq reaction buffer, 0.5 μL of 1 mM deoxynucleotide solution, 0.5 μL of each of the primers (10 μM), 0.125 μL of Taq DNA polymerase; selected volume of water or drugs diluted with water (sterile, ACS purity, Sigma-Aldrich) and 0.5 μL of bacteriophage λ DNA. .. The obtained DNA fragments (498 bp) were purified by MinElute PCR Purification Kit (Qiagen, Germany).

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Software:

    Article Title: A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    Article Snippet: The Taq DNA polymerase–antibody complex was prepared with ‘Jumpstart’ Taq DNA polymerase antibody (Sigma-Aldrich, Missouri, USA) according to instructions. .. Cycling conditions were as follows: Employing a Stratagene MX3000P instrument (Agilent Technologies, Stratagene, La Jolla, CA, USA) with software MXPro version 4.10, qLM–PCR reactions were heated to 94°C for 1 min to activate Taq DNA polymerase and release 12-mers, then ramped to 72°C for 4 min to re-anneal the target DNA and generate complementary sequence to the ligated 24-mers.

    Real-time Polymerase Chain Reaction:

    Article Title: Deficiency of AtGFAT1 activity impairs growth, pollen germination and tolerance to tunicamycin in Arabidopsis
    Article Snippet: Paragraph title: RNA isolation, RT-PCR and quantitative real-time PCR ... A 1 µl aliquot of the reverse transcribed reaction was used as a template for PCR with RED Taq DNA polymerase (Sigma-Aldrich, St Louis, MO, USA).

    Agarose Gel Electrophoresis:

    Article Title: Deficiency of AtGFAT1 activity impairs growth, pollen germination and tolerance to tunicamycin in Arabidopsis
    Article Snippet: A 1 µl aliquot of the reverse transcribed reaction was used as a template for PCR with RED Taq DNA polymerase (Sigma-Aldrich, St Louis, MO, USA). .. PCR cycling conditions were as follows: 94 °C for 1 min, followed by 25 cycles at 94 °C for 1 min, 62 °C for 30 s, and 72 °C for 30 s, and a final elongation step at 72 °C for 15 min. Amplified products were visualized on a 1.2% (w/v) agarose gel.

    Article Title: t-plasminogen activator inhibitor-1 polymorphism in idiopathic pulmonary arterial hypertension
    Article Snippet: A 12.5-µL PCR mixture was prepared containing 100 ng of genomic DNA, 0.2 mM of both forward and reverse primers, 10 mM dNTP's, 10XPCR buffer [10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2 mM MgCl2 ], 2.5% of formamide, 2U of Taq DNA polymerase (Sigma Aldrich, Germany). .. The products after digestion were electrophoresed on 2% agarose gel (Sigma Aldrich, Germany), at 150 V, containing 1 µL/10 mL ethidium bromide, and the genotypes were observed by visualizing under UV light.

    Article Title: Biochemical and Molecular Chitotriosidase Profiles in Patients with Gaucher Disease Type 1 in Minas Gerais, Brazil: New Mutation in CHIT1 Gene
    Article Snippet: The DNA was denatured at 95 °C for 2 min, and amplification was performed by 35 cycles at 95 °C for 20 s, 56 °C for 20 s and 72 °C for 30 s. The amplified fragments were separated by electrophoresis on a 2% agarose gel and visualized by ethidium bromide staining. .. The 40 μL reaction contained 1x PCR Buffer (Sigma-Aldrich Co., St. Louis, MO, USA), 1.5 mM dNTPs, 0.2 mM of each primer, 2.5 mM MgCl2 , 1 U Taq polymerase (Sigma-Aldrich Co., St. Louis, MO, USA), and 100 ng of DNA template.

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The amplified ITS region evaluated by 1% agarose gel electrophoresis with a 100bp marker obtained from Bangalore (Genei) and the amplified products were visualized by under a gel documentation system (Gel logic 2200 PRO).

    Article Title: p53 is necessary for the adaptive changes in cellular milieu subsequent to an acute bout of endurance exercise
    Article Snippet: Extracted DNA was added to a PCR tube containing DNA Taq polymerase (JumpStart REDtaq ReadyMix PCR reaction mix; Sigma-Aldrich, St. Louis, MO) and forward and reverse primers for the WT or the mutated p53 gene. .. The reaction products were separated on a 2% agarose gel at 90 V for 2–2.5 h and visualized with the use of ethidium bromide.

    Electrophoresis:

    Article Title: Biochemical and Molecular Chitotriosidase Profiles in Patients with Gaucher Disease Type 1 in Minas Gerais, Brazil: New Mutation in CHIT1 Gene
    Article Snippet: The DNA was denatured at 95 °C for 2 min, and amplification was performed by 35 cycles at 95 °C for 20 s, 56 °C for 20 s and 72 °C for 30 s. The amplified fragments were separated by electrophoresis on a 2% agarose gel and visualized by ethidium bromide staining. .. The 40 μL reaction contained 1x PCR Buffer (Sigma-Aldrich Co., St. Louis, MO, USA), 1.5 mM dNTPs, 0.2 mM of each primer, 2.5 mM MgCl2 , 1 U Taq polymerase (Sigma-Aldrich Co., St. Louis, MO, USA), and 100 ng of DNA template.

    Transgenic Assay:

    Article Title: p53 is necessary for the adaptive changes in cellular milieu subsequent to an acute bout of endurance exercise
    Article Snippet: Transgenic p53 mice ( ) were obtained from Taconic (Germantown, NY). .. Extracted DNA was added to a PCR tube containing DNA Taq polymerase (JumpStart REDtaq ReadyMix PCR reaction mix; Sigma-Aldrich, St. Louis, MO) and forward and reverse primers for the WT or the mutated p53 gene.

    Knock-Out:

    Article Title: p53 is necessary for the adaptive changes in cellular milieu subsequent to an acute bout of endurance exercise
    Article Snippet: Heterozygous p53 mice were bred to produce homozygous p53 knockout (KO) and littermate wild-type (WT) mice and were treated experimentally, as outlined in protocols approved by the York Animal Care Committee in accordance with the Canadian Council on Animal Care. .. Extracted DNA was added to a PCR tube containing DNA Taq polymerase (JumpStart REDtaq ReadyMix PCR reaction mix; Sigma-Aldrich, St. Louis, MO) and forward and reverse primers for the WT or the mutated p53 gene.

    Marker:

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The amplified ITS region evaluated by 1% agarose gel electrophoresis with a 100bp marker obtained from Bangalore (Genei) and the amplified products were visualized by under a gel documentation system (Gel logic 2200 PRO).

    Staining:

    Article Title: Biochemical and Molecular Chitotriosidase Profiles in Patients with Gaucher Disease Type 1 in Minas Gerais, Brazil: New Mutation in CHIT1 Gene
    Article Snippet: The DNA was denatured at 95 °C for 2 min, and amplification was performed by 35 cycles at 95 °C for 20 s, 56 °C for 20 s and 72 °C for 30 s. The amplified fragments were separated by electrophoresis on a 2% agarose gel and visualized by ethidium bromide staining. .. The 40 μL reaction contained 1x PCR Buffer (Sigma-Aldrich Co., St. Louis, MO, USA), 1.5 mM dNTPs, 0.2 mM of each primer, 2.5 mM MgCl2 , 1 U Taq polymerase (Sigma-Aldrich Co., St. Louis, MO, USA), and 100 ng of DNA template.

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  • 99
    Millipore ex taq polymerase
    Ex Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ex taq polymerase/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ex taq polymerase - by Bioz Stars, 2020-04
    99/100 stars
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