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Terumo Corp ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by Terumo Corp, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Terumo Corp
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
93/100 stars

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High Performance Liquid Chromatography:

Article Title: Relationship of reduced glomerular filtration rate with alterations in plasma free amino acids and uric acid evaluated in healthy control and hypertensive subjects
Article Snippet: Measurements of PFAA concentrations were performed by high-performance liquid chromatography–electrospray ionization–mass spectrometry (HPLC–ESI–MS) followed by precolumn derivatization which allows such measurements with high accuracy. .. Briefly, 5 ml of blood samples were collected into tubes containing ethylenediaminetetraacetic acid (EDTA; Terumo Co. Tokyo, Japan), which were put on ice immediately and kept for about 15 min. Then the tubes were centrifuged at 4 °C (3,000 rpm, 15 min) and the plasma was separated into tubes.

Centrifugation:

Article Title: Gpr158 mediates osteocalcin’s regulation of cognition
Article Snippet: .. Plasma was prepared from blood collected with EDTA into Capiject (T-MQK; Terumo) tubes followed by centrifugation at 1,000 g for 10 min. All plasma aliquots were stored at −80°C until use. .. Before administration, plasma was dialyzed using 3.5-kD D-tube dialyzers (71508-3; EMD Millipore) in PBS to remove EDTA.

Amplification:

Article Title: Hereditary lissencephaly and cerebellar hypoplasia in Churra lambs
Article Snippet: Pedigree data and analysis To confirm the pedigree information from the affected lambs and their parents we collected blood samples in a 10 ml Venoject tubes with EDTA (Terumo Europe N.V., Leuven, Belgium) of a total of 33 animals from the lissencephaly affected flock. .. All the samples were analysed for a set of 19 microsatellite markers amplified in a single PCR reaction following the Multiplex-PCR protocol described by [ ].

Chromatography:

Article Title: Relationship of reduced glomerular filtration rate with alterations in plasma free amino acids and uric acid evaluated in healthy control and hypertensive subjects
Article Snippet: Measurements of PFAA concentrations were performed by high-performance liquid chromatography–electrospray ionization–mass spectrometry (HPLC–ESI–MS) followed by precolumn derivatization which allows such measurements with high accuracy. .. Briefly, 5 ml of blood samples were collected into tubes containing ethylenediaminetetraacetic acid (EDTA; Terumo Co. Tokyo, Japan), which were put on ice immediately and kept for about 15 min. Then the tubes were centrifuged at 4 °C (3,000 rpm, 15 min) and the plasma was separated into tubes.

Protease Inhibitor:

Article Title: Selective Spectrum Antibiotic Modulation of the Gut Microbiome in Obesity and Diabetes Rodent Models
Article Snippet: Biochemical analysis Plasma and serum were prepared using EDTA and T-MG tubes, respectively [Terumo Medical Corporation, Elkton, MD]. .. EDTA tubes used to prepare plasma for hormone analysis contained 50 μM DPP IV inhibitor [Millipore, St. Charles, MO] and a protease inhibitor cocktail [Sigma-Aldrich, St. Louis, MO].

Multiplex PCR:

Article Title: Hereditary lissencephaly and cerebellar hypoplasia in Churra lambs
Article Snippet: Pedigree data and analysis To confirm the pedigree information from the affected lambs and their parents we collected blood samples in a 10 ml Venoject tubes with EDTA (Terumo Europe N.V., Leuven, Belgium) of a total of 33 animals from the lissencephaly affected flock. .. All the samples were analysed for a set of 19 microsatellite markers amplified in a single PCR reaction following the Multiplex-PCR protocol described by [ ].

Infection:

Article Title: Npro of classical swine fever virus contributes to pathogenicity in pigs by preventing type I interferon induction at local replication sites
Article Snippet: Paragraph title: Experimental infection of pigs ... With the 5 pigs kept for 14 days, blood was collected in tubes containing EDTA (Terumo, Tokyo, Japan) on days 0, 3, 5, 7, 9, 11 and 14 pi.

Mouse Assay:

Article Title: Gpr158 mediates osteocalcin’s regulation of cognition
Article Snippet: Plasma collection Pooled mouse plasma was collected from 3-mo-old WT or Ocn −/− mice or 16-mo-old WT mice by intracardial bleed at time of euthanasia. .. Plasma was prepared from blood collected with EDTA into Capiject (T-MQK; Terumo) tubes followed by centrifugation at 1,000 g for 10 min. All plasma aliquots were stored at −80°C until use.

Real-time Polymerase Chain Reaction:

Article Title: Stomoxys calcitrans, mechanical vector of virulent Besnoitia besnoiti from chronically infected cattle to susceptible rabbit
Article Snippet: .. Blood was collected in 4‐mL tubes containing EDTA (Terumo Europe NV) to assess the parasite load by qPCR and in 1‐mL tubes containing EDTA (Greiner Bio‐One GmbH, Kermsmünster, Austria) for haematological analysis. .. Serum samples collected in 3.5‐mL tubes containing silicone (Terumo Europe NV) were tested for B. besnoiti antibodies by in‐house WB using total B. besnoiti tachyzoite antigens and IFAT.

Concentration Assay:

Article Title: Relationship of reduced glomerular filtration rate with alterations in plasma free amino acids and uric acid evaluated in healthy control and hypertensive subjects
Article Snippet: Serum concentration of creatinine was measured by using the creatininase-HMMPS method following the manufacturer’s instructions (Wako Pure Chemical Industries, Osaka, Japan). .. Briefly, 5 ml of blood samples were collected into tubes containing ethylenediaminetetraacetic acid (EDTA; Terumo Co. Tokyo, Japan), which were put on ice immediately and kept for about 15 min. Then the tubes were centrifuged at 4 °C (3,000 rpm, 15 min) and the plasma was separated into tubes.

Incubation:

Article Title: Hematological and biochemical features of postpartum fever in the heavy draft mare
Article Snippet: Peripheral blood was collected into 10 ml vacuum tubes (Venoject II VP-P100K, Terumo Corp., Tokyo, Japan) and 5 ml vacuum tubes containing EDTA (Venoject II VP-NA050K, Terumo Corp.) by jugular venipuncture using 21 G × 1.5″ needles (MN-2138MS, Terumo Corp.). .. Plain tubes were centrifuged for 12 min at 3,000 rpm after incubation (37°C, 60 min).

other:

Article Title: Peripheral leukocyte populations and oxidative stress biomarkers in aged dogs showing impaired cognitive abilities
Article Snippet: At the end of the cognitive testing, the dog underwent a single blood collection using vacuum tubes with either EDTA or without anticoagulant (VENOJECT, Terumo Europe N.V., Leuven, Belgium).

Article Title: Acute Phase Proteins in Response to Dictyocaulus viviparus Infection in Calves
Article Snippet: Blood samples were obtained from the jugular vein in Venoject tubes with EDTA and without additive (Terumo Europe N.V., Leuven, Belgium).

Article Title: Dietary saturated fat/cholesterol, but not unsaturated fat or starch, induces C-reactive protein associated early atherosclerosis and ectopic fat deposition in diabetic pigs
Article Snippet: U. Lithium Heparin, 10 mL Venoject, Terumo, Leuven, Belgium) or EDTA (ethylenediaminetetraacetic acid, (0.47 mol/L EDTA, 10 mL Venoject, Terumo, Leuven, Belgium) tubes were immediately chilled at 0°C on water with ice, and centrifuged at 4°C for 10 minutes at 3000 rpm.

Cell Culture:

Article Title: Npro of classical swine fever virus contributes to pathogenicity in pigs by preventing type I interferon induction at local replication sites
Article Snippet: Experimental infection of pigs In order to assess the pathogenicity of the cDNA-derived viruses, groups of eight 4-week-old crossbred Landrace × Duroc × Yorkshire SPF pigs (Yamanaka Chikusan, Hokkaido, Japan) were injected intramuscularly with 1 mL of virus from cell culture supernatant. .. With the 5 pigs kept for 14 days, blood was collected in tubes containing EDTA (Terumo, Tokyo, Japan) on days 0, 3, 5, 7, 9, 11 and 14 pi.

Virus Isolation Assay:

Article Title: Npro of classical swine fever virus contributes to pathogenicity in pigs by preventing type I interferon induction at local replication sites
Article Snippet: With the 5 pigs kept for 14 days, blood was collected in tubes containing EDTA (Terumo, Tokyo, Japan) on days 0, 3, 5, 7, 9, 11 and 14 pi. .. The collected samples were homogenized in MEM to obtain a 10% suspension for virus isolation.

Polymerase Chain Reaction:

Article Title: Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat
Article Snippet: Paragraph title: PCR-inhibitory samples. ... The blood sample was drawn from a healthy person and placed into 5-ml evacuated blood collection tubes containing 0.1 ml (0.47 mol/liter) of EDTA (Terumo Europe N. V., Leuven, Belgium).

Article Title: Hereditary lissencephaly and cerebellar hypoplasia in Churra lambs
Article Snippet: Pedigree data and analysis To confirm the pedigree information from the affected lambs and their parents we collected blood samples in a 10 ml Venoject tubes with EDTA (Terumo Europe N.V., Leuven, Belgium) of a total of 33 animals from the lissencephaly affected flock. .. All the samples were analysed for a set of 19 microsatellite markers amplified in a single PCR reaction following the Multiplex-PCR protocol described by [ ].

Western Blot:

Article Title: Stomoxys calcitrans, mechanical vector of virulent Besnoitia besnoiti from chronically infected cattle to susceptible rabbit
Article Snippet: Blood was collected in 4‐mL tubes containing EDTA (Terumo Europe NV) to assess the parasite load by qPCR and in 1‐mL tubes containing EDTA (Greiner Bio‐One GmbH, Kermsmünster, Austria) for haematological analysis. .. Serum samples collected in 3.5‐mL tubes containing silicone (Terumo Europe NV) were tested for B. besnoiti antibodies by in‐house WB using total B. besnoiti tachyzoite antigens and IFAT.

Article Title: Gpr158 mediates osteocalcin’s regulation of cognition
Article Snippet: Plasma was prepared from blood collected with EDTA into Capiject (T-MQK; Terumo) tubes followed by centrifugation at 1,000 g for 10 min. All plasma aliquots were stored at −80°C until use. .. For Western blot analysis, mice were sacrificed to collect hippocampi 4 h after a single plasma injection.

Injection:

Article Title: Npro of classical swine fever virus contributes to pathogenicity in pigs by preventing type I interferon induction at local replication sites
Article Snippet: Experimental infection of pigs In order to assess the pathogenicity of the cDNA-derived viruses, groups of eight 4-week-old crossbred Landrace × Duroc × Yorkshire SPF pigs (Yamanaka Chikusan, Hokkaido, Japan) were injected intramuscularly with 1 mL of virus from cell culture supernatant. .. With the 5 pigs kept for 14 days, blood was collected in tubes containing EDTA (Terumo, Tokyo, Japan) on days 0, 3, 5, 7, 9, 11 and 14 pi.

Article Title: Gpr158 mediates osteocalcin’s regulation of cognition
Article Snippet: Plasma was prepared from blood collected with EDTA into Capiject (T-MQK; Terumo) tubes followed by centrifugation at 1,000 g for 10 min. All plasma aliquots were stored at −80°C until use. .. Mice were systemically treated with plasma (100 µl per injection) by injections into the tail vein eight times over 24 d before behavioral analysis.

Sampling:

Article Title: Role of TrkB expression in rat adrenal gland during acute immobilization stress
Article Snippet: .. Blood sampling After deep anesthesia of all rats at the end of experiments, blood samples were collected between 07:00 hours and 11:00 hours by cardiac puncture into Venoject II® tubes containing EDTA (Terumo, Tokyo, Japan). ..

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  • 93
    Terumo Corp edta 2na
    Microphotographs of whole blood passing through microchannel arrays Blood was sampled from the same healthy subject ( a , b ). a Adherent or clumped leukocytes (black arrows) were observed in whole blood with heparin in the microchannel flow analyser (MCFAN) by high-powered field (× 1400) . Deformed erythrocytes running through capillaries (white open arrows) were visible in the high-powered field ( a ). b There was no platelet aggregation and significantly fewer adherent leukocytes (black arrow) in blood with ethylene-diamine-tetraacetic acid <t>(EDTA)-2Na</t> + heparin because of Ca 2+ scavenging by EDTA-2Na. c The number of adhesive leukocytes was significantly lower ( p
    Edta 2na, supplied by Terumo Corp, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta 2na/product/Terumo Corp
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    edta 2na - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    94
    Terumo Corp edta 2k
    Influence of anticoagulant and blood preservation conditions on quality of cfDNA from healthy volunteers cfDNA concentrations were examined at the indicated time after blood collection using sodium citrate tubes ( A ) or <t>EDTA</t> 2K tubes ( B ) from ten healthy volunteers. Blood storage temperature until plasma separation was 4° C (white box) or room temperature (gray box). Size distribution of plasma DNA was analyzed with an Agilent bioanalyzer ® ; representative examples are shown in panels C-F. Sodium citrate tubes ( C , E ) or EDTA 2K tubes ( D , F ) were used for blood collection, and blood storage until plasma separation was at RT (C, D) or 4° C (E, F). DNA concentration of 1000 bp to 9000 bp fragments ( G ) and of 100 bp to 250 bp fragments ( H ) in all samples stored at 4° C was measured with an Agilent bioanalyzer ® as described in “Materials and methods”. Blood was collected into sodium citrate tubes (white box) or EDTA 2K tubes (gray box). Statistical analyses were performed with Friedman’s rank test.
    Edta 2k, supplied by Terumo Corp, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta 2k/product/Terumo Corp
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta 2k - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    92
    Terumo Corp edta
    Influence of anticoagulant and blood preservation conditions on quality of cfDNA from healthy volunteers cfDNA concentrations were examined at the indicated time after blood collection using sodium citrate tubes ( A ) or <t>EDTA</t> 2K tubes ( B ) from ten healthy volunteers. Blood storage temperature until plasma separation was 4° C (white box) or room temperature (gray box). Size distribution of plasma DNA was analyzed with an Agilent bioanalyzer ® ; representative examples are shown in panels C-F. Sodium citrate tubes ( C , E ) or EDTA 2K tubes ( D , F ) were used for blood collection, and blood storage until plasma separation was at RT (C, D) or 4° C (E, F). DNA concentration of 1000 bp to 9000 bp fragments ( G ) and of 100 bp to 250 bp fragments ( H ) in all samples stored at 4° C was measured with an Agilent bioanalyzer ® as described in “Materials and methods”. Blood was collected into sodium citrate tubes (white box) or EDTA 2K tubes (gray box). Statistical analyses were performed with Friedman’s rank test.
    Edta, supplied by Terumo Corp, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta/product/Terumo Corp
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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    Microphotographs of whole blood passing through microchannel arrays Blood was sampled from the same healthy subject ( a , b ). a Adherent or clumped leukocytes (black arrows) were observed in whole blood with heparin in the microchannel flow analyser (MCFAN) by high-powered field (× 1400) . Deformed erythrocytes running through capillaries (white open arrows) were visible in the high-powered field ( a ). b There was no platelet aggregation and significantly fewer adherent leukocytes (black arrow) in blood with ethylene-diamine-tetraacetic acid (EDTA)-2Na + heparin because of Ca 2+ scavenging by EDTA-2Na. c The number of adhesive leukocytes was significantly lower ( p

    Journal: Heart and Vessels

    Article Title: Clinically feasible method for assessing leukocyte rheology in whole blood

    doi: 10.1007/s00380-019-01486-y

    Figure Lengend Snippet: Microphotographs of whole blood passing through microchannel arrays Blood was sampled from the same healthy subject ( a , b ). a Adherent or clumped leukocytes (black arrows) were observed in whole blood with heparin in the microchannel flow analyser (MCFAN) by high-powered field (× 1400) . Deformed erythrocytes running through capillaries (white open arrows) were visible in the high-powered field ( a ). b There was no platelet aggregation and significantly fewer adherent leukocytes (black arrow) in blood with ethylene-diamine-tetraacetic acid (EDTA)-2Na + heparin because of Ca 2+ scavenging by EDTA-2Na. c The number of adhesive leukocytes was significantly lower ( p

    Article Snippet: Blood (10 mL) was sampled from the antecubital vein, and 5 mL was collected into each of two tubes: a 5% vol heparin tube (Venoject II, Terumo, Tokyo, Japan) and an EDTA-2Na + 5% vol heparin tube (Venoject II, Terumo, Tokyo, Japan).

    Techniques:

    Passage times of whole blood through microchannel arrays under − 25 cmH 2 O, − 30 cmH 2 O, and − 35 cmH 2 O. Standardized passage times of whole blood with heparin and whole blood with heparin + EDTA-2Na under a constant vacuum of 25 cmH 2 O, 30 cmH 2 O, or 35 cmH 2 O in 25 subjects ( a ). The passage time of whole blood with heparin under a constant vacuum of 25 cmH 2 O could not be measured in 19 of 25 subjects because of obstruction of the microchannel arrays. The passage time of whole blood with heparin + EDTA-2Na was shorter than that of whole blood with heparin under both 30 cmH 2 O and 35 cmH 2 O suction pressures. There was a significant correlation between change in whole blood passage time {(heparin) − (EDTA-2Na + heparin)} and adhesive leukocyte number (heparin) under − 30 cmH 2 O ( b r = 0.44, p = 0.029) and − 35 cmH 2 O ( c r = 0.49, p = 0.016)

    Journal: Heart and Vessels

    Article Title: Clinically feasible method for assessing leukocyte rheology in whole blood

    doi: 10.1007/s00380-019-01486-y

    Figure Lengend Snippet: Passage times of whole blood through microchannel arrays under − 25 cmH 2 O, − 30 cmH 2 O, and − 35 cmH 2 O. Standardized passage times of whole blood with heparin and whole blood with heparin + EDTA-2Na under a constant vacuum of 25 cmH 2 O, 30 cmH 2 O, or 35 cmH 2 O in 25 subjects ( a ). The passage time of whole blood with heparin under a constant vacuum of 25 cmH 2 O could not be measured in 19 of 25 subjects because of obstruction of the microchannel arrays. The passage time of whole blood with heparin + EDTA-2Na was shorter than that of whole blood with heparin under both 30 cmH 2 O and 35 cmH 2 O suction pressures. There was a significant correlation between change in whole blood passage time {(heparin) − (EDTA-2Na + heparin)} and adhesive leukocyte number (heparin) under − 30 cmH 2 O ( b r = 0.44, p = 0.029) and − 35 cmH 2 O ( c r = 0.49, p = 0.016)

    Article Snippet: Blood (10 mL) was sampled from the antecubital vein, and 5 mL was collected into each of two tubes: a 5% vol heparin tube (Venoject II, Terumo, Tokyo, Japan) and an EDTA-2Na + 5% vol heparin tube (Venoject II, Terumo, Tokyo, Japan).

    Techniques:

    Different pharmacological mechanisms of heparin and ethylene-diamine-tetraacetic acid (EDTA)-2Na in whole blood ex vivo . Pharmacological mechanisms of heparin and EDTA-2Na in whole blood ex vivo are demonstrated in this schematic. Heparin binds to the enzyme inhibitor antithrombin (AT) III and greatly accelerates the rate at which AT III inactivates the coagulation enzymes thrombin and factor Xa. In contrast, EDTA-2Na scavenges metal ions (Ca 2+ and Mg 2+ ), thereby inhibiting activation of platelets and Ca 2+ dependent activation of leukocytes. The heparinized blood tube maintained leukocyte and platelet function ex vivo , just as it did in vivo

    Journal: Heart and Vessels

    Article Title: Clinically feasible method for assessing leukocyte rheology in whole blood

    doi: 10.1007/s00380-019-01486-y

    Figure Lengend Snippet: Different pharmacological mechanisms of heparin and ethylene-diamine-tetraacetic acid (EDTA)-2Na in whole blood ex vivo . Pharmacological mechanisms of heparin and EDTA-2Na in whole blood ex vivo are demonstrated in this schematic. Heparin binds to the enzyme inhibitor antithrombin (AT) III and greatly accelerates the rate at which AT III inactivates the coagulation enzymes thrombin and factor Xa. In contrast, EDTA-2Na scavenges metal ions (Ca 2+ and Mg 2+ ), thereby inhibiting activation of platelets and Ca 2+ dependent activation of leukocytes. The heparinized blood tube maintained leukocyte and platelet function ex vivo , just as it did in vivo

    Article Snippet: Blood (10 mL) was sampled from the antecubital vein, and 5 mL was collected into each of two tubes: a 5% vol heparin tube (Venoject II, Terumo, Tokyo, Japan) and an EDTA-2Na + 5% vol heparin tube (Venoject II, Terumo, Tokyo, Japan).

    Techniques: Ex Vivo, Coagulation, Activation Assay, In Vivo

    Influence of anticoagulant and blood preservation conditions on quality of cfDNA from healthy volunteers cfDNA concentrations were examined at the indicated time after blood collection using sodium citrate tubes ( A ) or EDTA 2K tubes ( B ) from ten healthy volunteers. Blood storage temperature until plasma separation was 4° C (white box) or room temperature (gray box). Size distribution of plasma DNA was analyzed with an Agilent bioanalyzer ® ; representative examples are shown in panels C-F. Sodium citrate tubes ( C , E ) or EDTA 2K tubes ( D , F ) were used for blood collection, and blood storage until plasma separation was at RT (C, D) or 4° C (E, F). DNA concentration of 1000 bp to 9000 bp fragments ( G ) and of 100 bp to 250 bp fragments ( H ) in all samples stored at 4° C was measured with an Agilent bioanalyzer ® as described in “Materials and methods”. Blood was collected into sodium citrate tubes (white box) or EDTA 2K tubes (gray box). Statistical analyses were performed with Friedman’s rank test.

    Journal: Oncotarget

    Article Title: Investigation of appropriate pre-analytical procedure for circulating free DNA from liquid biopsy

    doi: 10.18632/oncotarget.25881

    Figure Lengend Snippet: Influence of anticoagulant and blood preservation conditions on quality of cfDNA from healthy volunteers cfDNA concentrations were examined at the indicated time after blood collection using sodium citrate tubes ( A ) or EDTA 2K tubes ( B ) from ten healthy volunteers. Blood storage temperature until plasma separation was 4° C (white box) or room temperature (gray box). Size distribution of plasma DNA was analyzed with an Agilent bioanalyzer ® ; representative examples are shown in panels C-F. Sodium citrate tubes ( C , E ) or EDTA 2K tubes ( D , F ) were used for blood collection, and blood storage until plasma separation was at RT (C, D) or 4° C (E, F). DNA concentration of 1000 bp to 9000 bp fragments ( G ) and of 100 bp to 250 bp fragments ( H ) in all samples stored at 4° C was measured with an Agilent bioanalyzer ® as described in “Materials and methods”. Blood was collected into sodium citrate tubes (white box) or EDTA 2K tubes (gray box). Statistical analyses were performed with Friedman’s rank test.

    Article Snippet: Blood collection tubes We used tubes containing 3.2% sodium citrate (TERUMO CORPRATION, Tokyo, Japan, product number VP-CA052K), called “sodium citrate tubes” in this paper, and tubes containing EDTA 2K (TERUMO CORPRATION, Tokyo, Japan, product number VP-DK052K05), called “EDTA 2K tubes” in this paper.

    Techniques: Preserving, Concentration Assay

    Schema for examining effects of anticoagulant and storage condition on quality of cfDNA A total of 32 ml blood was collected from each of ten healthy volunteers and divided into eight sodium citrate tubes and eight EDTA 2K tubes. Blood in each collection tube was stored at 4° C or RT for the indicated period. Plasma separation and cfDNA extraction were performed as shown in this figure. cfDNA quality was evaluated by measuring cfDNA concentration with Quantus ® and by analyzing cfDNA size distribution with Agilent Bioanalyzer ® .

    Journal: Oncotarget

    Article Title: Investigation of appropriate pre-analytical procedure for circulating free DNA from liquid biopsy

    doi: 10.18632/oncotarget.25881

    Figure Lengend Snippet: Schema for examining effects of anticoagulant and storage condition on quality of cfDNA A total of 32 ml blood was collected from each of ten healthy volunteers and divided into eight sodium citrate tubes and eight EDTA 2K tubes. Blood in each collection tube was stored at 4° C or RT for the indicated period. Plasma separation and cfDNA extraction were performed as shown in this figure. cfDNA quality was evaluated by measuring cfDNA concentration with Quantus ® and by analyzing cfDNA size distribution with Agilent Bioanalyzer ® .

    Article Snippet: Blood collection tubes We used tubes containing 3.2% sodium citrate (TERUMO CORPRATION, Tokyo, Japan, product number VP-CA052K), called “sodium citrate tubes” in this paper, and tubes containing EDTA 2K (TERUMO CORPRATION, Tokyo, Japan, product number VP-DK052K05), called “EDTA 2K tubes” in this paper.

    Techniques: Concentration Assay

    Influence of anticoagulant and blood preservation conditions on quality of cfDNA from healthy volunteers cfDNA concentrations were examined at the indicated time after blood collection using sodium citrate tubes ( A ) or EDTA 2K tubes ( B ) from ten healthy volunteers. Blood storage temperature until plasma separation was 4° C (white box) or room temperature (gray box). Size distribution of plasma DNA was analyzed with an Agilent bioanalyzer ® ; representative examples are shown in panels C-F. Sodium citrate tubes ( C , E ) or EDTA 2K tubes ( D , F ) were used for blood collection, and blood storage until plasma separation was at RT (C, D) or 4° C (E, F). DNA concentration of 1000 bp to 9000 bp fragments ( G ) and of 100 bp to 250 bp fragments ( H ) in all samples stored at 4° C was measured with an Agilent bioanalyzer ® as described in “Materials and methods”. Blood was collected into sodium citrate tubes (white box) or EDTA 2K tubes (gray box). Statistical analyses were performed with Friedman’s rank test.

    Journal: Oncotarget

    Article Title: Investigation of appropriate pre-analytical procedure for circulating free DNA from liquid biopsy

    doi: 10.18632/oncotarget.25881

    Figure Lengend Snippet: Influence of anticoagulant and blood preservation conditions on quality of cfDNA from healthy volunteers cfDNA concentrations were examined at the indicated time after blood collection using sodium citrate tubes ( A ) or EDTA 2K tubes ( B ) from ten healthy volunteers. Blood storage temperature until plasma separation was 4° C (white box) or room temperature (gray box). Size distribution of plasma DNA was analyzed with an Agilent bioanalyzer ® ; representative examples are shown in panels C-F. Sodium citrate tubes ( C , E ) or EDTA 2K tubes ( D , F ) were used for blood collection, and blood storage until plasma separation was at RT (C, D) or 4° C (E, F). DNA concentration of 1000 bp to 9000 bp fragments ( G ) and of 100 bp to 250 bp fragments ( H ) in all samples stored at 4° C was measured with an Agilent bioanalyzer ® as described in “Materials and methods”. Blood was collected into sodium citrate tubes (white box) or EDTA 2K tubes (gray box). Statistical analyses were performed with Friedman’s rank test.

    Article Snippet: We used tubes containing 3.2% sodium citrate (TERUMO CORPRATION, Tokyo, Japan, product number VP-CA052K), called “sodium citrate tubes” in this paper, and tubes containing EDTA 2K (TERUMO CORPRATION, Tokyo, Japan, product number VP-DK052K05), called “EDTA 2K tubes” in this paper.

    Techniques: Preserving, Concentration Assay

    Schema for examining effects of anticoagulant and storage condition on quality of cfDNA A total of 32 ml blood was collected from each of ten healthy volunteers and divided into eight sodium citrate tubes and eight EDTA 2K tubes. Blood in each collection tube was stored at 4° C or RT for the indicated period. Plasma separation and cfDNA extraction were performed as shown in this figure. cfDNA quality was evaluated by measuring cfDNA concentration with Quantus ® and by analyzing cfDNA size distribution with Agilent Bioanalyzer ® .

    Journal: Oncotarget

    Article Title: Investigation of appropriate pre-analytical procedure for circulating free DNA from liquid biopsy

    doi: 10.18632/oncotarget.25881

    Figure Lengend Snippet: Schema for examining effects of anticoagulant and storage condition on quality of cfDNA A total of 32 ml blood was collected from each of ten healthy volunteers and divided into eight sodium citrate tubes and eight EDTA 2K tubes. Blood in each collection tube was stored at 4° C or RT for the indicated period. Plasma separation and cfDNA extraction were performed as shown in this figure. cfDNA quality was evaluated by measuring cfDNA concentration with Quantus ® and by analyzing cfDNA size distribution with Agilent Bioanalyzer ® .

    Article Snippet: We used tubes containing 3.2% sodium citrate (TERUMO CORPRATION, Tokyo, Japan, product number VP-CA052K), called “sodium citrate tubes” in this paper, and tubes containing EDTA 2K (TERUMO CORPRATION, Tokyo, Japan, product number VP-DK052K05), called “EDTA 2K tubes” in this paper.

    Techniques: Concentration Assay