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Sinopharm ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by Sinopharm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Sinopharm
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
95/100 stars

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Related Articles

Synthesized:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: The primers used for PCR, synthesized by Sangon Biotech Co., Ltd. (Shanghai, China), were as follows: Sense, 5′-CTTTAAGAAGGCCACGGTGA-3′ and anti-sense, 5′-TCCAAGGGTCTCCTTCAGTG-3′ for PDGF-BB; sense, 5′-AAGACATCGATGCCTCAAAC-3′ and anti-sense, 5′-CTCCAGTAGGCTGTGAAGTG-3′ for CD44 ( ); and sense, 5′-TATCCAGGCTGTGCTATCCC-3′ and anti-sense, 5′-CCATCTCTTGCTCGAAGTCC-3′ for β-actin. .. Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich).

Micro-CT:

Article Title: Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration
Article Snippet: A Micro-CT scanner (Skyscan 1176, Kontich, Belgium) was used to reconstruct images using software, and by which, the relative bone volume fraction (BV/TV) and the bone mineral density (BMD) were affirmed. .. When they were decalcified in 10% ethylenediaminetetraacetic acid (EDTA; Sinopharm Chemical Reagent Co. Ltd) for 2 weeks, the calvarial samples were embedded in paraffin and sectioned at 5 μm at the central area of the defect.

Electrophoresis:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich). .. Wide Mini-Sub Cell GT Horizontal Electrophoresis System and PowerPac™ Universal Power Supply (Bio-Rad Laboratories, Inc., Hercules, CA, USA) were applied for gel electrophoresis, with voltage and time set at 100 V and 20 min, respectively.

Expressing:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich). .. DNA fragments were visualized and quantified using ChemiDoc MP system (Bio-Rad Laboratories, Inc.), and relative amounts of CD44 transcripts were determined against β-actin expression.

Modification:

Article Title: Characterization of the role of the photosensitizer, deuteporfin, in the detection of lymphatic metastases in a pancreatic cancer xenograft model
Article Snippet: .. Absolute ethanol, high glucose-Dulbecco's modified Eagle's medium (H-DMEM), fetal bovine serum (FBS), trypsin, EDTA, formalin, paraffin, and hematoxylin and eosin (H & E) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). .. In addition, deionized water was from Fudan University (Shanghai, China), and Tris-buffered saline and phosphate-buffered saline were prepared in our laboratory.

High Performance Liquid Chromatography:

Article Title: A Novel Integrative Processing Technology for the Preparation of Rehmanniae Radix Slices
Article Snippet: Methanol (for HPLC) and acetonitrile (for HPLC) were purchased from Merck (Darmstadt, Germany). .. Ethanol absolute, croscarmellose sodium (CMC-Na), and EDTA were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Article Title: Periostin identified as a potential biomarker of prostate cancer by iTRAQ-proteomics analysis of prostate biopsy
Article Snippet: KCl, KH2PO4, NaCl, Tris, EDTA, Triton X-100, SDS were obtained from Sinopharm Chemical Reagent Co, Ltd (Shanghai, China). .. 20AD HPLC system (Shimadzu, Japan).

Article Title: Development and in vitro/in vivo Evaluation of a Silastic Intravaginal Ring for Mifepristone Delivery
Article Snippet: EDTA, hydrochloric acid, dehydrated alcohol and sodium dihydrogen phosphate were obtained from Sinopharm Chemical Reagent Beijing Co. Ltd., Beijing, China. .. HPLC grade acetonitrile was purchased from Fisher Scientific, Fair Lawn, NJ, USA.

Flow Cytometry:

Article Title: Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration
Article Snippet: To evaluate blood vessel formation at 8 weeks post-surgery, the rats were perfused with Microfil (Microfil MV-122; Flow Tech, Carver, MA) after euthanasia. .. When they were decalcified in 10% ethylenediaminetetraacetic acid (EDTA; Sinopharm Chemical Reagent Co. Ltd) for 2 weeks, the calvarial samples were embedded in paraffin and sectioned at 5 μm at the central area of the defect.

Generated:

Article Title: Periostin identified as a potential biomarker of prostate cancer by iTRAQ-proteomics analysis of prostate biopsy
Article Snippet: KCl, KH2PO4, NaCl, Tris, EDTA, Triton X-100, SDS were obtained from Sinopharm Chemical Reagent Co, Ltd (Shanghai, China). .. All buffers were prepared with the ultrapure water generated from the Milli-Q system (Millipore, USA).

other:

Article Title: The effect of desulfurization on the postharvest quality and sulfite metabolism in pulp of sulfitated “Feizixiao” Litchi (Litchi chinensis Sonn.) fruits. The effect of desulfurization on the postharvest quality and sulfite metabolism in pulp of sulfitated “Feizixiao” Litchi (Litchi chinensis Sonn.) fruits
Article Snippet: 2.2 Chemicals ZnSO4 , NaOH, H2 SO4 , sodium borate, formaldehyde, pararosaniline, sodium tetrachloromercurate, DTT, KCl, EDTA, EGTA, glycerol, Tris(hydroxymethyl) aminomethane, EDTA‐Na2 , HCl, K3 Fe(CN)6 , Na2 SO3 , AMP, cysteine, Triton X‐100, PVP40, HEPES, NADPH, 5,5′‐dithiobis‐(2‐nitrobenzoic acid) (DTNB), acetyl‐CoA, l ‐serine, MgCl2 , Tween 80, PVP, OAS, ninhydrin hydrate, acetonitrile, and methanol were analytically pure and all provided by Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).

Article Title: Synthesis of High Valence Silver-Loaded Mesoporous Silica with Strong Antibacterial Properties
Article Snippet: Tetraethoxysilane (TEOS), hydrochloric acid (HCl, 37%), EDTA, thionyl chloride (SOCl2 ), toluene, dichloromethane (DCM), ether, acetone, sodium carbonate solution, aqueous sodium bicarbonate (NaHCO3 ), nitric acid, sodium hydroxide (NaOH), and lead nitrate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Reverse Transcription Polymerase Chain Reaction:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: Paragraph title: Reverse transcription-polymerase chain reaction (RT-PCR) ... Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich).

Injection:

Article Title: Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration
Article Snippet: To obtain new bone formation and mineralization rate, we were put to death in 8 weeks of polychrome sequential fluorescent labeling in rats, 2 weeks, 4 weeks and 6 weeks after surgery, after the ether anesthesia to the animals and intraperitoneal injection of fluorescent composed of 30 mg/kg alizarin red (AL) (Sigma, USA), 25 mg/kg tetracycline (TE), and 20 mg/kg calcein (CA) (Sigma, USA). .. When they were decalcified in 10% ethylenediaminetetraacetic acid (EDTA; Sinopharm Chemical Reagent Co. Ltd) for 2 weeks, the calvarial samples were embedded in paraffin and sectioned at 5 μm at the central area of the defect.

Recombinant:

Article Title: Identification, structure modification, and characterization of potential small-molecule SGK3 inhibitors with novel scaffolds
Article Snippet: MgCl2 , EGTA, EDTA, Tris, and Tween-20 were bought from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). .. The SGK3 recombinant human protein used for the HTS campaign was procured from Life Technologies (Carlsbad, CA, USA), and the assay plates (ProxiPlate-384 F Plus) were acquired from PerkinElmer.

Immunofluorescence:

Article Title: Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration
Article Snippet: When they were decalcified in 10% ethylenediaminetetraacetic acid (EDTA; Sinopharm Chemical Reagent Co. Ltd) for 2 weeks, the calvarial samples were embedded in paraffin and sectioned at 5 μm at the central area of the defect. .. Immunohistochemistry and immunofluorescence staining for CD31 (ab24590, 1:200; Abcam) and osteocalcin (OCN, ab13420, 1:100; Abcam) were then performed in the sections from each group.

Nucleic Acid Electrophoresis:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich). .. Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich).

In Vivo:

Article Title: Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration
Article Snippet: Paragraph title: The capacity of osteogenesis and angiogenic of Co-BG scaffold in vivo ... When they were decalcified in 10% ethylenediaminetetraacetic acid (EDTA; Sinopharm Chemical Reagent Co. Ltd) for 2 weeks, the calvarial samples were embedded in paraffin and sectioned at 5 μm at the central area of the defect.

Immunohistochemistry:

Article Title: Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration
Article Snippet: When they were decalcified in 10% ethylenediaminetetraacetic acid (EDTA; Sinopharm Chemical Reagent Co. Ltd) for 2 weeks, the calvarial samples were embedded in paraffin and sectioned at 5 μm at the central area of the defect. .. Immunohistochemistry and immunofluorescence staining for CD31 (ab24590, 1:200; Abcam) and osteocalcin (OCN, ab13420, 1:100; Abcam) were then performed in the sections from each group.

Labeling:

Article Title: Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration
Article Snippet: To obtain new bone formation and mineralization rate, we were put to death in 8 weeks of polychrome sequential fluorescent labeling in rats, 2 weeks, 4 weeks and 6 weeks after surgery, after the ether anesthesia to the animals and intraperitoneal injection of fluorescent composed of 30 mg/kg alizarin red (AL) (Sigma, USA), 25 mg/kg tetracycline (TE), and 20 mg/kg calcein (CA) (Sigma, USA). .. When they were decalcified in 10% ethylenediaminetetraacetic acid (EDTA; Sinopharm Chemical Reagent Co. Ltd) for 2 weeks, the calvarial samples were embedded in paraffin and sectioned at 5 μm at the central area of the defect.

Purification:

Article Title: The Influence of Materials, Heterostructure, and Orientation for Nanohybrids on Photocatalytic Activity
Article Snippet: .. Indium tin oxide (ITO)-coated glass (CSG Holding Co., Ltd., 15 Ω/sq), zinc nitrate (Zn(NO3 )·6H2 O), hexamethylenetetramine (HMT), copper (II) sulfate pentahydrate, sodium hydroxide, lactic acid, potassium thiocyanate, ethylenediaminetetraacetic acid, triethanolamine, and nickel nitrate hexahydrate are all purchased from Sinopharm Chemical Reagent Co., Ltd. All these materials are of analytical grade and used as received without further purification. ..

Article Title: Removal of Carmine from Aqueous Solution by Carbonated Hydroxyapatite Nanorods
Article Snippet: .. Materials Ca(NO3 )2 ·4H2 O (AR, Sinopharm Chemical Reagent, Shanghai, China), (NH4 )2 HPO4 (AR, Sinopharm Chemical Reagent, Shanghai, China), CTAB (AR, Sinopharm Chemical Reagent, Shanghai, China), and EDTA (AR, Sinopharm Chemical Reagent, Shanghai, China) were used as received without further purification. .. Samples Synthesis In the typical hydrothermal synthesis of CHA nanorods, Ca(NO3 )2 ·4H2 O and (NH4 )2 HPO4 were used as calcium source and phosphorus source, respectively.

Article Title: Characterization of the role of the photosensitizer, deuteporfin, in the detection of lymphatic metastases in a pancreatic cancer xenograft model
Article Snippet: Deuteporfin was obtained from the R & D Center of Fudan-Zhangjiang Bio-Pharmaceutical Co. Ltd. (Shanghai, China) and was used without further purification. .. Absolute ethanol, high glucose-Dulbecco's modified Eagle's medium (H-DMEM), fetal bovine serum (FBS), trypsin, EDTA, formalin, paraffin, and hematoxylin and eosin (H & E) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Polymerase Chain Reaction:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich). .. Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich).

Software:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: The bands were scanned using ChemiImager 5500 version 2.03 software (Alpha Innotech, San Leandro, CA, USA). .. Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich).

Agarose Gel Electrophoresis:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: .. Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich). .. Wide Mini-Sub Cell GT Horizontal Electrophoresis System and PowerPac™ Universal Power Supply (Bio-Rad Laboratories, Inc., Hercules, CA, USA) were applied for gel electrophoresis, with voltage and time set at 100 V and 20 min, respectively.

Size-exclusion Chromatography:

Article Title: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Article Snippet: PCR was performed under the following conditions for PDGF-BB: Denaturation at 94°C for 46 min, followed by 40 cycles of 94°C for 15 sec, 62°C for 1 min and 72°C for 1 min, with a final extension step at 72°C for 7 min; PCR was performed under the following conditions for CD44: Denaturation for 95°C for 15 min, followed by 45 cycles of 94°C for 15 sec, 55°C for 30 sec and 72°C for 30 sec. .. Agarose gel, which was prepared in 1×TAE buffer containing 40 mM Tris-acetic acid (pH 8.5; Tris-base was purchased from Sigma Aldrich; acetic acid was from Sinopharm Chemical Reagent Co., Ltd.) and 2 mM ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd.), was supplemented with 0.5 μg/mL ethidium bromide (Sigma-Aldrich).

Concentration Assay:

Article Title: PDL regeneration via cell homing in delayed replantation of avulsed teeth
Article Snippet: In Group A, the roots were first treated with 17 % EDTA (Sinopharm Chemical Reagent, Shanghai, China) for 24 h to achieve partial demineralization. .. At the same time, SDF1 and BMP7 were both dissolved at a same concentration of 100 ng/mL in a neutralized type I collagen (2 mg/mL) solution (ProSpec, Israel).

Staining:

Article Title: Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration
Article Snippet: Histological staining observation image was examined by Image Pro PlusTM (Media Cybernetics, Silver Springs, MD). .. When they were decalcified in 10% ethylenediaminetetraacetic acid (EDTA; Sinopharm Chemical Reagent Co. Ltd) for 2 weeks, the calvarial samples were embedded in paraffin and sectioned at 5 μm at the central area of the defect.

HMT Assay:

Article Title: The Influence of Materials, Heterostructure, and Orientation for Nanohybrids on Photocatalytic Activity
Article Snippet: .. Indium tin oxide (ITO)-coated glass (CSG Holding Co., Ltd., 15 Ω/sq), zinc nitrate (Zn(NO3 )·6H2 O), hexamethylenetetramine (HMT), copper (II) sulfate pentahydrate, sodium hydroxide, lactic acid, potassium thiocyanate, ethylenediaminetetraacetic acid, triethanolamine, and nickel nitrate hexahydrate are all purchased from Sinopharm Chemical Reagent Co., Ltd. All these materials are of analytical grade and used as received without further purification. ..

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    Sinopharm chemicals na2 edta 2h2 o
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    Average 90 stars, based on 1 article reviews
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    Sinopharm ethylenediaminetetraacetic acid edta
    Ethylenediaminetetraacetic Acid Edta, supplied by Sinopharm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacetic acid edta/product/Sinopharm
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetic acid edta - by Bioz Stars, 2020-04
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      Buy from Supplier

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