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Merck & Co ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by Merck & Co, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Centrifugation:

Article Title: Vaccinia Virus Penetration Requires Cholesterol and Results in Specific Viral Envelope Proteins Associated with Lipid Rafts
Article Snippet: At the indicated time points (0, 15, 30, and 60 min), the cells were scraped with a rubber policeman into ice-cold PBS and pelleted by centrifugation at 200 × g for 3 min at 4°C. .. The cell pellet was lysed with 0.3 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche).

Article Title: Mint3 potentiates TLR3/4- and RIG-I–induced IFN-β expression and antiviral immune responses
Article Snippet: For immunoprecipitation (IP), whole-cell extracts were lysed in IP buffer containing 1.0% (vol/vol) Nonidet P 40, 50 mM Tris⋅HCl, pH 7.4, 50 mM EDTA, 150 mM NaCl, and a protease inhibitor “mixture” (Merck). .. After centrifugation for 10 min at 14,000 × g , supernatants were collected and incubated with protein G Plus-Agrose Immunoprecipitation reagent together with specific antibody.

Article Title: Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration
Article Snippet: For proteome analyses isolated mucus samples were incubated overnight at 37°C in reduction buffer (6M guanidini um hydrochloride (GuHCl), 0.1M Tris/HCl, pH 8.5 (Merck), 5mM EDTA, 0.1 MDTT (Merck)) and all liquid added on top of a spin-filter (10 kDa, PALL, Port Washington, NY) for a filter-aided sample preparation following a previous protocol ( ) where 6M GuHCl was used instead of urea. .. Peptides released from the filter after centrifugation were cleaned with StageTip C18 columns ( ).

Filtration:

Article Title: The Trypsin Inhibitor Panulirin Regulates the Prophenoloxidase-activating System in the Spiny Lobster Panulirus argus
Article Snippet: DMSO, EDTA, NaCl, Tris, DTT, acetonitrile (LiChrosolv®, hypergrade for LC/MS), TFA (for protein sequence analysis grade), papain from Carica papaya (EC 3.4.22.2), Triton X-100, protamine sulfate, glucose, sodium citrate, and calcium chloride were all obtained from Merck. .. The HiTrap SP HP column, the low molecular weight calibration kit for SDS electrophoresis, and the low molecular weight gel filtration calibration kit were from GE Healthcare.

Expressing:

Article Title: Evolutionary Patterning: A Novel Approach to the Identification of Potential Drug Target Sites in Plasmodium falciparum
Article Snippet: Paragraph title: Expression and purification of recombinant GST-PfGK (rPfGK) ... 1/100 (v/v) culture was added to LB and cells were allowed to grow to an OD600nm ≥0.6 at 37°C before induction with 1mM isopropyl thio-β-D-galactoside (IPTG, Promega, USA) for 6 hours at 37°C. rPfGK was predominantly expressed as insoluble protein trapped in inclusion bodies in E. coli and was purified with BugBuster® HT (Novagen, USA) as per manufacturer's instructions. rPfGK was denatured in 6M guanidine hydrochloride, 50mM Tris HCl pH 8.0, 100mM NaCl, 10mM EDTA and 10mM DTT (Merck, USA) at 4°C overnight.

Synthesized:

Article Title: The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1
Article Snippet: Reactions were stopped by adding up to 50 mM EDTA and analyzed on PEI cellulose thin-layer chromatography plates (Merck) in 0.75 M LiCl (1 µl/sample). .. The in vitro synthesized RNA (127 nucleotides) probe was labeled with [γ-32 P]GTP using the ScriptCap m7 G Capping System and the ScriptCap 2’-O -Methyltransferase kit (EPICENTRE Biotechnologies).

Autoradiography:

Article Title: l-Selectin activates the Ras pathway via the tyrosine kinase p56lck
Article Snippet: .. IPs were washed eight times in lysis buffer, resuspended in 20 μl EDTA (1 mM), incubated for 20 min at 68°C, and eluted nucleotides were separated on a thin layer chromatography polyethyleneimine-cellulose plate (Merck) with 0.75 M KH2 PO4 (pH 3.5), followed by autoradiography. .. Jurkat cells were transiently transfected with 40 μg transdominant inhibitory N17Ras (pEF-N17 ras ) or control vector (pEF) and 8 μg of a CD20 expression vector (pRc/CMV- cd20 ).

Electrophoresis:

Article Title: The Trypsin Inhibitor Panulirin Regulates the Prophenoloxidase-activating System in the Spiny Lobster Panulirus argus
Article Snippet: DMSO, EDTA, NaCl, Tris, DTT, acetonitrile (LiChrosolv®, hypergrade for LC/MS), TFA (for protein sequence analysis grade), papain from Carica papaya (EC 3.4.22.2), Triton X-100, protamine sulfate, glucose, sodium citrate, and calcium chloride were all obtained from Merck. .. The HiTrap SP HP column, the low molecular weight calibration kit for SDS electrophoresis, and the low molecular weight gel filtration calibration kit were from GE Healthcare.

Incubation:

Article Title: The Lipid Raft-Associated Protein CD98 Is Required for Vaccinia Virus Endocytosis
Article Snippet: HeLa cells (108 ) were mock infected with serum-free medium or infected with VV in serum-free medium at a multiplicity of infection (MOI) of 20 PFU per cell for 30 min at 37°C, washed twice with ice-cold phosphate-buffered saline (PBS) to remove unbound virions, and lysed with 5.0 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche). .. The cells were further incubated at 4°C for 30 min with gentle agitation, and the cell lysates were then centrifuged for 10 min at 3,000 rpm at 4°C in an Eppendorf 5415C centrifuge to remove nuclei and insoluble materials.

Article Title: l-Selectin activates the Ras pathway via the tyrosine kinase p56lck
Article Snippet: .. IPs were washed eight times in lysis buffer, resuspended in 20 μl EDTA (1 mM), incubated for 20 min at 68°C, and eluted nucleotides were separated on a thin layer chromatography polyethyleneimine-cellulose plate (Merck) with 0.75 M KH2 PO4 (pH 3.5), followed by autoradiography. .. Jurkat cells were transiently transfected with 40 μg transdominant inhibitory N17Ras (pEF-N17 ras ) or control vector (pEF) and 8 μg of a CD20 expression vector (pRc/CMV- cd20 ).

Article Title: The molecular architecture of the metalloprotease FtsH
Article Snippet: .. For inhibition of ATPase activity, 50 mM EDTA was added to the samples and incubated for 1 h. Samples were taken at different time points and separated by thin-layer chromatography (PEI-Cellulose F; Merck), run in 0.2 M KH2 PO4 , 0.33 M HCOOH, and 0.19 M LiCl, and analyzed by using a phosphoimager (FLA-3000, Fujifilm). ..

Article Title: Vaccinia Virus Penetration Requires Cholesterol and Results in Specific Viral Envelope Proteins Associated with Lipid Rafts
Article Snippet: HeLa cells (8 × 106 ) were mock infected or infected with VV at an MOI of 25 PFU per cell for 1 h at 4°C, washed twice with ice-cold PBS to remove unbound virions, and incubated with prewarmed serum-free medium at 37°C. .. The cell pellet was lysed with 0.3 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche).

Article Title: Mint3 potentiates TLR3/4- and RIG-I–induced IFN-β expression and antiviral immune responses
Article Snippet: For immunoprecipitation (IP), whole-cell extracts were lysed in IP buffer containing 1.0% (vol/vol) Nonidet P 40, 50 mM Tris⋅HCl, pH 7.4, 50 mM EDTA, 150 mM NaCl, and a protease inhibitor “mixture” (Merck). .. After centrifugation for 10 min at 14,000 × g , supernatants were collected and incubated with protein G Plus-Agrose Immunoprecipitation reagent together with specific antibody.

Article Title: Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration
Article Snippet: .. For proteome analyses isolated mucus samples were incubated overnight at 37°C in reduction buffer (6M guanidini um hydrochloride (GuHCl), 0.1M Tris/HCl, pH 8.5 (Merck), 5mM EDTA, 0.1 MDTT (Merck)) and all liquid added on top of a spin-filter (10 kDa, PALL, Port Washington, NY) for a filter-aided sample preparation following a previous protocol ( ) where 6M GuHCl was used instead of urea. .. Proteins were alkylated on 10 kDa cut-off filters and subsequently digested for 4h with LysC (Wako, Richmond, VA) followed by an overnight trypsin (Promega, Fitchburg, WI) digestion.

Activity Assay:

Article Title: Human apoA-I increases macrophage foam cell derived PLTP activity without affecting the PLTP mass
Article Snippet: .. The fractions containing PLTP activity were combined, dialysed against 25 mM Tris-HCl buffer, pH = 7.40, containing 1 mM EDTA, and applied to a Mono Q HR 5/5 column, attached to a Merck HPLC system. ..

Article Title: The molecular architecture of the metalloprotease FtsH
Article Snippet: .. For inhibition of ATPase activity, 50 mM EDTA was added to the samples and incubated for 1 h. Samples were taken at different time points and separated by thin-layer chromatography (PEI-Cellulose F; Merck), run in 0.2 M KH2 PO4 , 0.33 M HCOOH, and 0.19 M LiCl, and analyzed by using a phosphoimager (FLA-3000, Fujifilm). ..

Mass Spectrometry:

Article Title: The Lipid Raft-Associated Protein CD98 Is Required for Vaccinia Virus Endocytosis
Article Snippet: Paragraph title: Isolation of low-density detergent-insoluble membrane fractions on flotation gradients for differential IMID-H4/IMID-D4 labeling and high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) analyses. ... HeLa cells (108 ) were mock infected with serum-free medium or infected with VV in serum-free medium at a multiplicity of infection (MOI) of 20 PFU per cell for 30 min at 37°C, washed twice with ice-cold phosphate-buffered saline (PBS) to remove unbound virions, and lysed with 5.0 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche).

Ex Vivo:

Article Title: Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration
Article Snippet: After ex vivo mucus measurements mucus was collected, 2X cOmplete Protease Inhibitor Cocktail (Merck) was added and samples were stored at −80ºC until further processing. .. For proteome analyses isolated mucus samples were incubated overnight at 37°C in reduction buffer (6M guanidini um hydrochloride (GuHCl), 0.1M Tris/HCl, pH 8.5 (Merck), 5mM EDTA, 0.1 MDTT (Merck)) and all liquid added on top of a spin-filter (10 kDa, PALL, Port Washington, NY) for a filter-aided sample preparation following a previous protocol ( ) where 6M GuHCl was used instead of urea.

Western Blot:

Article Title: Mint3 potentiates TLR3/4- and RIG-I–induced IFN-β expression and antiviral immune responses
Article Snippet: Paragraph title: Immunoprecipitation and Western Blot. ... For immunoprecipitation (IP), whole-cell extracts were lysed in IP buffer containing 1.0% (vol/vol) Nonidet P 40, 50 mM Tris⋅HCl, pH 7.4, 50 mM EDTA, 150 mM NaCl, and a protease inhibitor “mixture” (Merck).

Article Title: Genotoxicity evaluation of hydroalcoholic and aqueous extracts of Dorema aucheri by the comet assay
Article Snippet: Materials Tris, Triton X-100, H2 O2 , NaCl, EDTA, NaOH and NaH2 PO4 (Merck Co., Germany), low-melting-point agarose (LMA), Na2 HPO4 , KCl and Ethidium bromide (Sigma Co. USA), normal melting point agarose (NMA) (Cinnagen Co., Iran), RPMI-1640, FBS and antibiotic (PAA Co., Australia) were used in this study. .. The aerial parts of D. aucheri were collected from Yasuj Mountains in the western Iran at the end of spring 2012.

Transformation Assay:

Article Title: Evolutionary Patterning: A Novel Approach to the Identification of Potential Drug Target Sites in Plasmodium falciparum
Article Snippet: Transformed cells were selected in LB medium containing 100 μg/ml ampicillin and 50 μg/ml chloramphenicol (Roche, Germany). .. 1/100 (v/v) culture was added to LB and cells were allowed to grow to an OD600nm ≥0.6 at 37°C before induction with 1mM isopropyl thio-β-D-galactoside (IPTG, Promega, USA) for 6 hours at 37°C. rPfGK was predominantly expressed as insoluble protein trapped in inclusion bodies in E. coli and was purified with BugBuster® HT (Novagen, USA) as per manufacturer's instructions. rPfGK was denatured in 6M guanidine hydrochloride, 50mM Tris HCl pH 8.0, 100mM NaCl, 10mM EDTA and 10mM DTT (Merck, USA) at 4°C overnight.

High Performance Liquid Chromatography:

Article Title: Human apoA-I increases macrophage foam cell derived PLTP activity without affecting the PLTP mass
Article Snippet: .. The fractions containing PLTP activity were combined, dialysed against 25 mM Tris-HCl buffer, pH = 7.40, containing 1 mM EDTA, and applied to a Mono Q HR 5/5 column, attached to a Merck HPLC system. ..

Chromatography:

Article Title: The Lipid Raft-Associated Protein CD98 Is Required for Vaccinia Virus Endocytosis
Article Snippet: Paragraph title: Isolation of low-density detergent-insoluble membrane fractions on flotation gradients for differential IMID-H4/IMID-D4 labeling and high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) analyses. ... HeLa cells (108 ) were mock infected with serum-free medium or infected with VV in serum-free medium at a multiplicity of infection (MOI) of 20 PFU per cell for 30 min at 37°C, washed twice with ice-cold phosphate-buffered saline (PBS) to remove unbound virions, and lysed with 5.0 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche).

Immunoprecipitation:

Article Title: l-Selectin activates the Ras pathway via the tyrosine kinase p56lck
Article Snippet: Ras or Rac2 were immunoprecipitated by Ras Y13-259 or Rac2 antibodies followed by the addition of anti-rat- or anti-rabbit-coupled agarose. .. IPs were washed eight times in lysis buffer, resuspended in 20 μl EDTA (1 mM), incubated for 20 min at 68°C, and eluted nucleotides were separated on a thin layer chromatography polyethyleneimine-cellulose plate (Merck) with 0.75 M KH2 PO4 (pH 3.5), followed by autoradiography.

Article Title: Mint3 potentiates TLR3/4- and RIG-I–induced IFN-β expression and antiviral immune responses
Article Snippet: .. For immunoprecipitation (IP), whole-cell extracts were lysed in IP buffer containing 1.0% (vol/vol) Nonidet P 40, 50 mM Tris⋅HCl, pH 7.4, 50 mM EDTA, 150 mM NaCl, and a protease inhibitor “mixture” (Merck). .. After centrifugation for 10 min at 14,000 × g , supernatants were collected and incubated with protein G Plus-Agrose Immunoprecipitation reagent together with specific antibody.

Protease Inhibitor:

Article Title: Mint3 potentiates TLR3/4- and RIG-I–induced IFN-β expression and antiviral immune responses
Article Snippet: .. For immunoprecipitation (IP), whole-cell extracts were lysed in IP buffer containing 1.0% (vol/vol) Nonidet P 40, 50 mM Tris⋅HCl, pH 7.4, 50 mM EDTA, 150 mM NaCl, and a protease inhibitor “mixture” (Merck). .. After centrifugation for 10 min at 14,000 × g , supernatants were collected and incubated with protein G Plus-Agrose Immunoprecipitation reagent together with specific antibody.

Article Title: Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration
Article Snippet: After ex vivo mucus measurements mucus was collected, 2X cOmplete Protease Inhibitor Cocktail (Merck) was added and samples were stored at −80ºC until further processing. .. For proteome analyses isolated mucus samples were incubated overnight at 37°C in reduction buffer (6M guanidini um hydrochloride (GuHCl), 0.1M Tris/HCl, pH 8.5 (Merck), 5mM EDTA, 0.1 MDTT (Merck)) and all liquid added on top of a spin-filter (10 kDa, PALL, Port Washington, NY) for a filter-aided sample preparation following a previous protocol ( ) where 6M GuHCl was used instead of urea.

Infection:

Article Title: The Lipid Raft-Associated Protein CD98 Is Required for Vaccinia Virus Endocytosis
Article Snippet: .. HeLa cells (108 ) were mock infected with serum-free medium or infected with VV in serum-free medium at a multiplicity of infection (MOI) of 20 PFU per cell for 30 min at 37°C, washed twice with ice-cold phosphate-buffered saline (PBS) to remove unbound virions, and lysed with 5.0 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche). .. The cells were further incubated at 4°C for 30 min with gentle agitation, and the cell lysates were then centrifuged for 10 min at 3,000 rpm at 4°C in an Eppendorf 5415C centrifuge to remove nuclei and insoluble materials.

Article Title: Vaccinia Virus Penetration Requires Cholesterol and Results in Specific Viral Envelope Proteins Associated with Lipid Rafts
Article Snippet: HeLa cells (8 × 106 ) were mock infected or infected with VV at an MOI of 25 PFU per cell for 1 h at 4°C, washed twice with ice-cold PBS to remove unbound virions, and incubated with prewarmed serum-free medium at 37°C. .. The cell pellet was lysed with 0.3 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche).

Inhibition:

Article Title: The molecular architecture of the metalloprotease FtsH
Article Snippet: .. For inhibition of ATPase activity, 50 mM EDTA was added to the samples and incubated for 1 h. Samples were taken at different time points and separated by thin-layer chromatography (PEI-Cellulose F; Merck), run in 0.2 M KH2 PO4 , 0.33 M HCOOH, and 0.19 M LiCl, and analyzed by using a phosphoimager (FLA-3000, Fujifilm). ..

Sequencing:

Article Title: The Trypsin Inhibitor Panulirin Regulates the Prophenoloxidase-activating System in the Spiny Lobster Panulirus argus
Article Snippet: .. DMSO, EDTA, NaCl, Tris, DTT, acetonitrile (LiChrosolv®, hypergrade for LC/MS), TFA (for protein sequence analysis grade), papain from Carica papaya (EC 3.4.22.2), Triton X-100, protamine sulfate, glucose, sodium citrate, and calcium chloride were all obtained from Merck. ..

Recombinant:

Article Title: Evolutionary Patterning: A Novel Approach to the Identification of Potential Drug Target Sites in Plasmodium falciparum
Article Snippet: Paragraph title: Expression and purification of recombinant GST-PfGK (rPfGK) ... 1/100 (v/v) culture was added to LB and cells were allowed to grow to an OD600nm ≥0.6 at 37°C before induction with 1mM isopropyl thio-β-D-galactoside (IPTG, Promega, USA) for 6 hours at 37°C. rPfGK was predominantly expressed as insoluble protein trapped in inclusion bodies in E. coli and was purified with BugBuster® HT (Novagen, USA) as per manufacturer's instructions. rPfGK was denatured in 6M guanidine hydrochloride, 50mM Tris HCl pH 8.0, 100mM NaCl, 10mM EDTA and 10mM DTT (Merck, USA) at 4°C overnight.

Molecular Weight:

Article Title: The Trypsin Inhibitor Panulirin Regulates the Prophenoloxidase-activating System in the Spiny Lobster Panulirus argus
Article Snippet: DMSO, EDTA, NaCl, Tris, DTT, acetonitrile (LiChrosolv®, hypergrade for LC/MS), TFA (for protein sequence analysis grade), papain from Carica papaya (EC 3.4.22.2), Triton X-100, protamine sulfate, glucose, sodium citrate, and calcium chloride were all obtained from Merck. .. The HiTrap SP HP column, the low molecular weight calibration kit for SDS electrophoresis, and the low molecular weight gel filtration calibration kit were from GE Healthcare.

Isolation:

Article Title: The Lipid Raft-Associated Protein CD98 Is Required for Vaccinia Virus Endocytosis
Article Snippet: Paragraph title: Isolation of low-density detergent-insoluble membrane fractions on flotation gradients for differential IMID-H4/IMID-D4 labeling and high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) analyses. ... HeLa cells (108 ) were mock infected with serum-free medium or infected with VV in serum-free medium at a multiplicity of infection (MOI) of 20 PFU per cell for 30 min at 37°C, washed twice with ice-cold phosphate-buffered saline (PBS) to remove unbound virions, and lysed with 5.0 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche).

Article Title: Vaccinia Virus Penetration Requires Cholesterol and Results in Specific Viral Envelope Proteins Associated with Lipid Rafts
Article Snippet: Paragraph title: Isolation of low-density detergent-insoluble membrane fractions on flotation gradients. ... The cell pellet was lysed with 0.3 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche).

Article Title: Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration
Article Snippet: .. For proteome analyses isolated mucus samples were incubated overnight at 37°C in reduction buffer (6M guanidini um hydrochloride (GuHCl), 0.1M Tris/HCl, pH 8.5 (Merck), 5mM EDTA, 0.1 MDTT (Merck)) and all liquid added on top of a spin-filter (10 kDa, PALL, Port Washington, NY) for a filter-aided sample preparation following a previous protocol ( ) where 6M GuHCl was used instead of urea. .. Proteins were alkylated on 10 kDa cut-off filters and subsequently digested for 4h with LysC (Wako, Richmond, VA) followed by an overnight trypsin (Promega, Fitchburg, WI) digestion.

Labeling:

Article Title: The Lipid Raft-Associated Protein CD98 Is Required for Vaccinia Virus Endocytosis
Article Snippet: Paragraph title: Isolation of low-density detergent-insoluble membrane fractions on flotation gradients for differential IMID-H4/IMID-D4 labeling and high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) analyses. ... HeLa cells (108 ) were mock infected with serum-free medium or infected with VV in serum-free medium at a multiplicity of infection (MOI) of 20 PFU per cell for 30 min at 37°C, washed twice with ice-cold phosphate-buffered saline (PBS) to remove unbound virions, and lysed with 5.0 ml of ice-cold TNE buffer (25 mM Tris [pH 7.5], 150 mM NaCl, 5 mM EDTA) containing 1% Triton X-100 (Merck), 1 mM NaF, and a cocktail of protease inhibitors (Roche).

Article Title: l-Selectin activates the Ras pathway via the tyrosine kinase p56lck
Article Snippet: Cells were metabolically labeled for 4 h with 1 mCi/ml [32 Pi ] (1 Ci = 37 GBq) in phosphate-free DMEM/10% FCS, activated via l -selectin and lysed in 0.1% SDS, 0.5% deoxycholate, 1% Triton X-100, 450 mM NaCl, 10 mM NaF, 25 mM Hepes (pH 7.4), 20 mM MgCl2 , and 20 μg/ml aprotinin/leupeptin. .. IPs were washed eight times in lysis buffer, resuspended in 20 μl EDTA (1 mM), incubated for 20 min at 68°C, and eluted nucleotides were separated on a thin layer chromatography polyethyleneimine-cellulose plate (Merck) with 0.75 M KH2 PO4 (pH 3.5), followed by autoradiography.

Article Title: The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1
Article Snippet: Reactions were stopped by adding up to 50 mM EDTA and analyzed on PEI cellulose thin-layer chromatography plates (Merck) in 0.75 M LiCl (1 µl/sample). .. The in vitro synthesized RNA (127 nucleotides) probe was labeled with [γ-32 P]GTP using the ScriptCap m7 G Capping System and the ScriptCap 2’-O -Methyltransferase kit (EPICENTRE Biotechnologies).

Purification:

Article Title: Human apoA-I increases macrophage foam cell derived PLTP activity without affecting the PLTP mass
Article Snippet: Paragraph title: Purification of PLTP from human plasma ... The fractions containing PLTP activity were combined, dialysed against 25 mM Tris-HCl buffer, pH = 7.40, containing 1 mM EDTA, and applied to a Mono Q HR 5/5 column, attached to a Merck HPLC system.

Article Title: Evolutionary Patterning: A Novel Approach to the Identification of Potential Drug Target Sites in Plasmodium falciparum
Article Snippet: .. 1/100 (v/v) culture was added to LB and cells were allowed to grow to an OD600nm ≥0.6 at 37°C before induction with 1mM isopropyl thio-β-D-galactoside (IPTG, Promega, USA) for 6 hours at 37°C. rPfGK was predominantly expressed as insoluble protein trapped in inclusion bodies in E. coli and was purified with BugBuster® HT (Novagen, USA) as per manufacturer's instructions. rPfGK was denatured in 6M guanidine hydrochloride, 50mM Tris HCl pH 8.0, 100mM NaCl, 10mM EDTA and 10mM DTT (Merck, USA) at 4°C overnight. .. The sample was diluted to 3M guanidine hydrochloride with refolding buffer (200mM Tris-HCl pH 8.0, 10mM EDTA, 1M L-arginine, 0.1mM PMSF, 2mM reduced glutathione, 0.2mM oxidized glutathione, Merck, USA).

Metabolic Labelling:

Article Title: l-Selectin activates the Ras pathway via the tyrosine kinase p56lck
Article Snippet: Cells were metabolically labeled for 4 h with 1 mCi/ml [32 Pi ] (1 Ci = 37 GBq) in phosphate-free DMEM/10% FCS, activated via l -selectin and lysed in 0.1% SDS, 0.5% deoxycholate, 1% Triton X-100, 450 mM NaCl, 10 mM NaF, 25 mM Hepes (pH 7.4), 20 mM MgCl2 , and 20 μg/ml aprotinin/leupeptin. .. IPs were washed eight times in lysis buffer, resuspended in 20 μl EDTA (1 mM), incubated for 20 min at 68°C, and eluted nucleotides were separated on a thin layer chromatography polyethyleneimine-cellulose plate (Merck) with 0.75 M KH2 PO4 (pH 3.5), followed by autoradiography.

ATPase Assay:

Article Title: The molecular architecture of the metalloprotease FtsH
Article Snippet: Paragraph title: ATPase Assay. ... For inhibition of ATPase activity, 50 mM EDTA was added to the samples and incubated for 1 h. Samples were taken at different time points and separated by thin-layer chromatography (PEI-Cellulose F; Merck), run in 0.2 M KH2 PO4 , 0.33 M HCOOH, and 0.19 M LiCl, and analyzed by using a phosphoimager (FLA-3000, Fujifilm).

Enzyme-linked Immunosorbent Assay:

Article Title: Study on genotoxicity, oxidative stress biomarkers and clinical symptoms in workers of an asbestos-cement factory
Article Snippet: .. Materials and Methods Thiobarbituric acid (TBA) (Sigma-Aldrich, Chemie Gmbh, Munich, Germany), trichloroacetic acid (TCA), n-butanol, 2,4,6-tripyridyl striazine (TPTZ), HNO3 , H2 SO4 , HCL, NaCl, PdCl2 , DTNB (2-nitrobenzoic acid), EDTA, CH3 OH, Na(SO4 ), sodium hypochlorite, acetic acid, sodium acetate, ethanol from Merck (Tehran, Iran), and human specific ELISA kits for 8-OH-dG from Cayman Chemical Co. (Michigan, USA) were used in this research. ..

Affinity Chromatography:

Article Title: Evolutionary Patterning: A Novel Approach to the Identification of Potential Drug Target Sites in Plasmodium falciparum
Article Snippet: 1/100 (v/v) culture was added to LB and cells were allowed to grow to an OD600nm ≥0.6 at 37°C before induction with 1mM isopropyl thio-β-D-galactoside (IPTG, Promega, USA) for 6 hours at 37°C. rPfGK was predominantly expressed as insoluble protein trapped in inclusion bodies in E. coli and was purified with BugBuster® HT (Novagen, USA) as per manufacturer's instructions. rPfGK was denatured in 6M guanidine hydrochloride, 50mM Tris HCl pH 8.0, 100mM NaCl, 10mM EDTA and 10mM DTT (Merck, USA) at 4°C overnight. .. The refolded GST-PfGK was purified by affinity chromatography with GST°Mag™ Agarose beads (Promega, USA).

Sample Prep:

Article Title: Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration
Article Snippet: .. For proteome analyses isolated mucus samples were incubated overnight at 37°C in reduction buffer (6M guanidini um hydrochloride (GuHCl), 0.1M Tris/HCl, pH 8.5 (Merck), 5mM EDTA, 0.1 MDTT (Merck)) and all liquid added on top of a spin-filter (10 kDa, PALL, Port Washington, NY) for a filter-aided sample preparation following a previous protocol ( ) where 6M GuHCl was used instead of urea. .. Proteins were alkylated on 10 kDa cut-off filters and subsequently digested for 4h with LysC (Wako, Richmond, VA) followed by an overnight trypsin (Promega, Fitchburg, WI) digestion.

In Vitro:

Article Title: The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1
Article Snippet: Reactions were stopped by adding up to 50 mM EDTA and analyzed on PEI cellulose thin-layer chromatography plates (Merck) in 0.75 M LiCl (1 µl/sample). .. The in vitro synthesized RNA (127 nucleotides) probe was labeled with [γ-32 P]GTP using the ScriptCap m7 G Capping System and the ScriptCap 2’-O -Methyltransferase kit (EPICENTRE Biotechnologies).

Activation Assay:

Article Title: l-Selectin activates the Ras pathway via the tyrosine kinase p56lck
Article Snippet: Paragraph title: Ras and Rac2 Activation. ... IPs were washed eight times in lysis buffer, resuspended in 20 μl EDTA (1 mM), incubated for 20 min at 68°C, and eluted nucleotides were separated on a thin layer chromatography polyethyleneimine-cellulose plate (Merck) with 0.75 M KH2 PO4 (pH 3.5), followed by autoradiography.

Thin Layer Chromatography:

Article Title: l-Selectin activates the Ras pathway via the tyrosine kinase p56lck
Article Snippet: .. IPs were washed eight times in lysis buffer, resuspended in 20 μl EDTA (1 mM), incubated for 20 min at 68°C, and eluted nucleotides were separated on a thin layer chromatography polyethyleneimine-cellulose plate (Merck) with 0.75 M KH2 PO4 (pH 3.5), followed by autoradiography. .. Jurkat cells were transiently transfected with 40 μg transdominant inhibitory N17Ras (pEF-N17 ras ) or control vector (pEF) and 8 μg of a CD20 expression vector (pRc/CMV- cd20 ).

Article Title: The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1
Article Snippet: .. Reactions were stopped by adding up to 50 mM EDTA and analyzed on PEI cellulose thin-layer chromatography plates (Merck) in 0.75 M LiCl (1 µl/sample). .. The in vitro synthesized RNA (127 nucleotides) probe was labeled with [γ-32 P]GTP using the ScriptCap m7 G Capping System and the ScriptCap 2’-O -Methyltransferase kit (EPICENTRE Biotechnologies).

Article Title: The molecular architecture of the metalloprotease FtsH
Article Snippet: .. For inhibition of ATPase activity, 50 mM EDTA was added to the samples and incubated for 1 h. Samples were taken at different time points and separated by thin-layer chromatography (PEI-Cellulose F; Merck), run in 0.2 M KH2 PO4 , 0.33 M HCOOH, and 0.19 M LiCl, and analyzed by using a phosphoimager (FLA-3000, Fujifilm). ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: The Trypsin Inhibitor Panulirin Regulates the Prophenoloxidase-activating System in the Spiny Lobster Panulirus argus
Article Snippet: .. DMSO, EDTA, NaCl, Tris, DTT, acetonitrile (LiChrosolv®, hypergrade for LC/MS), TFA (for protein sequence analysis grade), papain from Carica papaya (EC 3.4.22.2), Triton X-100, protamine sulfate, glucose, sodium citrate, and calcium chloride were all obtained from Merck. ..

Lysis:

Article Title: l-Selectin activates the Ras pathway via the tyrosine kinase p56lck
Article Snippet: .. IPs were washed eight times in lysis buffer, resuspended in 20 μl EDTA (1 mM), incubated for 20 min at 68°C, and eluted nucleotides were separated on a thin layer chromatography polyethyleneimine-cellulose plate (Merck) with 0.75 M KH2 PO4 (pH 3.5), followed by autoradiography. .. Jurkat cells were transiently transfected with 40 μg transdominant inhibitory N17Ras (pEF-N17 ras ) or control vector (pEF) and 8 μg of a CD20 expression vector (pRc/CMV- cd20 ).

other:

Article Title: Morphology and electronic structure of nanoscale powders of calcium hydroxyapatite
Article Snippet: EDTA (Merck) was used as a chelating agent to prevent an immediate precipitate formation calcium ion in the course of gel formation.

Article Title: Pyrene removal from contaminated soils by modified Fenton oxidation using iron nano particles
Article Snippet: Hydrogen peroxide (30%), H2 SO4 (98%), HCl, NaOH, EDTA, sodium citrate, sodium pyrophosphate, HgCl2 , FeSO4 .7H2 O were also purchased from Merck Company.

Article Title: Combination of ERG9 Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene in Saccharomyces cerevisiae
Article Snippet: Batch Fermentation Batch fermentation was carried out in a controlled bioreactor (Spectrochem, India) containing 2 L mineral medium that consists of (g/L) galactose 20; (NH4 )2 SO4 , 5; KH2 PO4 , 3; MgSO4 7H2 O, 0.5; EDTA, 0.015; ZnSO4 ·7H2 O, 0.0045; CoC12 ·6H2 O, 0.0003; MnC12 4H2 O, 0.001; CuSO4 5H2 O, 0.0003; CaC12 ·2H2 O, 0.0000045; FeSO4 ·7H2 O, 0.0003; NaMoO4 ,·2H2 O, 0.0004; H3 BO3 , 0.001; KI, 0.0001; and 0.025 mL silicone antifoam (Merck).

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  • 99
    Merck & Co ethylenediaminetetraacetic acid edta
    Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)
    Ethylenediaminetetraacetic Acid Edta, supplied by Merck & Co, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co caspase 3 assay buffer
    TIMP-3 induced apoptosis shows independence of FASL. A. hVSMCs were transduced with control adenovirus (RAd60) or adenovirus expressing TIMP-3 (RAdT3) for 48 h before staining with 2 μg ml -1 isotype control IgG or an anti-FASL antibody and an anti-IgG Alexfluor488 conjugate. Cell-surface alexafluor488 mean fluorescence staining intensity was measured using flowcytometry. Data is the mean ± SEM, n = 3. NS = Not Significant. B. hVSMCs were transduced with control adenovirus (RAd60) or adenovirus expressing TIMP-3 (RAdT3) for 16 h before the addition of PBS (vehicle control), 2 μg ml -1 isotype control IgG or a function blocking anti-FASL antibody. The antibody was added every 24 h before cell lysates were harvested 72 h post adenoviral transduction and <t>caspase-3</t> activity measured. Data is the mean ± SEM, n = 3. *** = P
    Caspase 3 Assay Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co aβ1 42 oligomers
    Analysis of inflammation in the hippocampus after intracerebroventricular injection of oligomerized <t>Aβ1–42</t> in the cerebral ventricles. a – c , mRNA expression analysis of Il1 β ( a ), Il6 ( b ), and Tnf ( c ) in the hippocampus 6
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    Merck & Co tris acetate edta tae buffer
    Cleavage of SC pUC19 DNA (50 μ M) by platinum complex (100 μ M) in the presence of H 2 O 2 (100 μ M), in 10 mM <t>Tris-HCl/1</t> mM <t>EDTA</t> buffer (pH 8.0). lane 1: DNA Marker; lane 2: DNA control; lane 3: DNA + complex; lane 4: DNA + complex 1 + H 2 O 2 ; lane 5: DNA + complex + H 2 O 2 + DMSO (4 μ L); lane 6: DNA + complex 1 + H 2 O 2 + NaN 3 (100 μ M).
    Tris Acetate Edta Tae Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and ethylenediaminetetraacetic acid (EDTA) (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Conessine as a novel inhibitor of multidrug efflux pump systems in Pseudomonas aeruginosa

    doi: 10.1186/s12906-017-1913-y

    Figure Lengend Snippet: Intracellular concentration of 1- N -phenylnaphthylamine in the presence of conessine (20 mg/L) and ethylenediaminetetraacetic acid (EDTA) (100 μM) in Pseudomonas aeruginosa K767 (PAO1) ( a ), P. aeruginosa K1455 (PAO1- nalB ) ( b ), and P. aeruginosa K1523 (PAO1-∆ mexB ) ( c ). Data were shown as average of two independent experiments. Error bars displayed ±SEM and * indicated significant ( P value ≤0.05)

    Article Snippet: Dimethylsulfoxide (DMSO) and ethylenediaminetetraacetic acid (EDTA) were obtained from Merck (Merck, Germany).

    Techniques: Concentration Assay

    TIMP-3 induced apoptosis shows independence of FASL. A. hVSMCs were transduced with control adenovirus (RAd60) or adenovirus expressing TIMP-3 (RAdT3) for 48 h before staining with 2 μg ml -1 isotype control IgG or an anti-FASL antibody and an anti-IgG Alexfluor488 conjugate. Cell-surface alexafluor488 mean fluorescence staining intensity was measured using flowcytometry. Data is the mean ± SEM, n = 3. NS = Not Significant. B. hVSMCs were transduced with control adenovirus (RAd60) or adenovirus expressing TIMP-3 (RAdT3) for 16 h before the addition of PBS (vehicle control), 2 μg ml -1 isotype control IgG or a function blocking anti-FASL antibody. The antibody was added every 24 h before cell lysates were harvested 72 h post adenoviral transduction and caspase-3 activity measured. Data is the mean ± SEM, n = 3. *** = P

    Journal: PLoS ONE

    Article Title: Tissue Inhibitor of Metalloproteinase–3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells

    doi: 10.1371/journal.pone.0195116

    Figure Lengend Snippet: TIMP-3 induced apoptosis shows independence of FASL. A. hVSMCs were transduced with control adenovirus (RAd60) or adenovirus expressing TIMP-3 (RAdT3) for 48 h before staining with 2 μg ml -1 isotype control IgG or an anti-FASL antibody and an anti-IgG Alexfluor488 conjugate. Cell-surface alexafluor488 mean fluorescence staining intensity was measured using flowcytometry. Data is the mean ± SEM, n = 3. NS = Not Significant. B. hVSMCs were transduced with control adenovirus (RAd60) or adenovirus expressing TIMP-3 (RAdT3) for 16 h before the addition of PBS (vehicle control), 2 μg ml -1 isotype control IgG or a function blocking anti-FASL antibody. The antibody was added every 24 h before cell lysates were harvested 72 h post adenoviral transduction and caspase-3 activity measured. Data is the mean ± SEM, n = 3. *** = P

    Article Snippet: Samples were split into duplicate wells for each biological replicate and were incubated with caspase-3 assay buffer (50 mM Tris-HCl, pH 7.4, 5 mM EDTA, 1 mM DTT, 1 μM caspase-3 fluorogenic substrate IX (Merck) and Complete™ proteinase inhibitor cocktail (Roche) with and without 1 μM Z-VAD-CHO (Caspase Inhibitor II, Merck) at 37°C for 16 h. Fluorescence was measured in a TECAN Spectrafluor Plus microtitre plate reader at 485 nm and 595 nm.

    Techniques: Transduction, Expressing, Staining, Fluorescence, Blocking Assay, Activity Assay

    TIMP-3 overexpression induces apoptosis via a type-I death receptor pathway in hVSMCs. A. Western blot of lysates of VSMCs infected with either control (RAd60) or TIMP-3 (RAdT3)-expressing adenovirus harvested at the indicated times. Western blots were probed with anti-caspase-8, which can detect the pro, processed and 18 kDa active forms (Casp-8 and p18 Casp-8 respectively), anti-caspase-9 (Casp-9), anti-TIMP-3 and anti-beta-actin as a loading control. B and C. hVSMCs were infected with control (RAd60) or TIMP-3 (RAdT3)-expressing adenovirus followed by control (RAd60), Bcl2 (RAd-Bcl2), CrmA (RAd-CrmA) or dominant negative FADD (RAd-DN-FADD)-expressing adenovirus as described in the experimental procedures. Lysates were harvested after 72 h and analysed by Western blot to confirm expression of Bcl2, CrmA, DN-FADD and TIMP-3 with anti-beta-actin as a loading control (B) or caspase-3 activity measured (C). Data in C are the mean ± SEM, n = 5, *** = P

    Journal: PLoS ONE

    Article Title: Tissue Inhibitor of Metalloproteinase–3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells

    doi: 10.1371/journal.pone.0195116

    Figure Lengend Snippet: TIMP-3 overexpression induces apoptosis via a type-I death receptor pathway in hVSMCs. A. Western blot of lysates of VSMCs infected with either control (RAd60) or TIMP-3 (RAdT3)-expressing adenovirus harvested at the indicated times. Western blots were probed with anti-caspase-8, which can detect the pro, processed and 18 kDa active forms (Casp-8 and p18 Casp-8 respectively), anti-caspase-9 (Casp-9), anti-TIMP-3 and anti-beta-actin as a loading control. B and C. hVSMCs were infected with control (RAd60) or TIMP-3 (RAdT3)-expressing adenovirus followed by control (RAd60), Bcl2 (RAd-Bcl2), CrmA (RAd-CrmA) or dominant negative FADD (RAd-DN-FADD)-expressing adenovirus as described in the experimental procedures. Lysates were harvested after 72 h and analysed by Western blot to confirm expression of Bcl2, CrmA, DN-FADD and TIMP-3 with anti-beta-actin as a loading control (B) or caspase-3 activity measured (C). Data in C are the mean ± SEM, n = 5, *** = P

    Article Snippet: Samples were split into duplicate wells for each biological replicate and were incubated with caspase-3 assay buffer (50 mM Tris-HCl, pH 7.4, 5 mM EDTA, 1 mM DTT, 1 μM caspase-3 fluorogenic substrate IX (Merck) and Complete™ proteinase inhibitor cocktail (Roche) with and without 1 μM Z-VAD-CHO (Caspase Inhibitor II, Merck) at 37°C for 16 h. Fluorescence was measured in a TECAN Spectrafluor Plus microtitre plate reader at 485 nm and 595 nm.

    Techniques: Over Expression, Western Blot, Infection, Expressing, Dominant Negative Mutation, Activity Assay

    TIMP-3-induced apoptosis in hVSMCs is dependent on FAS, increases cellular FAS protein levels and induces DISC formation. A. Western blots of hVSMCs (Cont), transduced with lentivirus carrying puromycin resistance alone (Puro), expressing non-targeting control shRNA (shCont), or shRNA targeting FAS (FAS-1, -3, -4). B. Cells from (A) were infected with control (RAd60) or TIMP-3-expressing adenovirus (RAdT3) and caspase-3 activity analysed after 72 h. Data are the mean ± standard deviation, n = 3, *** = p

    Journal: PLoS ONE

    Article Title: Tissue Inhibitor of Metalloproteinase–3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells

    doi: 10.1371/journal.pone.0195116

    Figure Lengend Snippet: TIMP-3-induced apoptosis in hVSMCs is dependent on FAS, increases cellular FAS protein levels and induces DISC formation. A. Western blots of hVSMCs (Cont), transduced with lentivirus carrying puromycin resistance alone (Puro), expressing non-targeting control shRNA (shCont), or shRNA targeting FAS (FAS-1, -3, -4). B. Cells from (A) were infected with control (RAd60) or TIMP-3-expressing adenovirus (RAdT3) and caspase-3 activity analysed after 72 h. Data are the mean ± standard deviation, n = 3, *** = p

    Article Snippet: Samples were split into duplicate wells for each biological replicate and were incubated with caspase-3 assay buffer (50 mM Tris-HCl, pH 7.4, 5 mM EDTA, 1 mM DTT, 1 μM caspase-3 fluorogenic substrate IX (Merck) and Complete™ proteinase inhibitor cocktail (Roche) with and without 1 μM Z-VAD-CHO (Caspase Inhibitor II, Merck) at 37°C for 16 h. Fluorescence was measured in a TECAN Spectrafluor Plus microtitre plate reader at 485 nm and 595 nm.

    Techniques: Western Blot, Transduction, Expressing, shRNA, Infection, Activity Assay, Standard Deviation

    Analysis of inflammation in the hippocampus after intracerebroventricular injection of oligomerized Aβ1–42 in the cerebral ventricles. a – c , mRNA expression analysis of Il1 β ( a ), Il6 ( b ), and Tnf ( c ) in the hippocampus 6

    Journal: The Journal of Neuroscience

    Article Title: Amyloid β Oligomers Disrupt Blood–CSF Barrier Integrity by Activating Matrix Metalloproteinases

    doi: 10.1523/JNEUROSCI.0006-15.2015

    Figure Lengend Snippet: Analysis of inflammation in the hippocampus after intracerebroventricular injection of oligomerized Aβ1–42 in the cerebral ventricles. a – c , mRNA expression analysis of Il1 β ( a ), Il6 ( b ), and Tnf ( c ) in the hippocampus 6

    Article Snippet: To assess the role of MMPs, we used three treatments: Aβ1–42 oligomers (in Tris-EDTA buffer) combined with 1 μg of broad spectrum MMP inhibitor GM6001 (Merck; catalog #CC1100) dissolved in DMSO, Aβ1–42 oligomers (in Tris-EDTA buffer) combined with DMSO and vehicle for the control group.

    Techniques: Injection, Expressing

    Analysis of the effect of Aβ1–42 oligomers on BBB integrity. a , Relative permeability of the BBB 6 h after intracerebroventricular injection of Aβ1–42 oligomers (gray) compared with scrambled peptide injected mice (black)

    Journal: The Journal of Neuroscience

    Article Title: Amyloid β Oligomers Disrupt Blood–CSF Barrier Integrity by Activating Matrix Metalloproteinases

    doi: 10.1523/JNEUROSCI.0006-15.2015

    Figure Lengend Snippet: Analysis of the effect of Aβ1–42 oligomers on BBB integrity. a , Relative permeability of the BBB 6 h after intracerebroventricular injection of Aβ1–42 oligomers (gray) compared with scrambled peptide injected mice (black)

    Article Snippet: To assess the role of MMPs, we used three treatments: Aβ1–42 oligomers (in Tris-EDTA buffer) combined with 1 μg of broad spectrum MMP inhibitor GM6001 (Merck; catalog #CC1100) dissolved in DMSO, Aβ1–42 oligomers (in Tris-EDTA buffer) combined with DMSO and vehicle for the control group.

    Techniques: Permeability, Injection, Mouse Assay

    Analysis of Aβ1–42 oligomer-induced disruption of BCSFB integrity. a , Relative BCSFB permeability 2 and 6 h after intracerebroventricular injection of Aβ1–42 oligomers in the cerebral ventricles (gray) compared with control

    Journal: The Journal of Neuroscience

    Article Title: Amyloid β Oligomers Disrupt Blood–CSF Barrier Integrity by Activating Matrix Metalloproteinases

    doi: 10.1523/JNEUROSCI.0006-15.2015

    Figure Lengend Snippet: Analysis of Aβ1–42 oligomer-induced disruption of BCSFB integrity. a , Relative BCSFB permeability 2 and 6 h after intracerebroventricular injection of Aβ1–42 oligomers in the cerebral ventricles (gray) compared with control

    Article Snippet: To assess the role of MMPs, we used three treatments: Aβ1–42 oligomers (in Tris-EDTA buffer) combined with 1 μg of broad spectrum MMP inhibitor GM6001 (Merck; catalog #CC1100) dissolved in DMSO, Aβ1–42 oligomers (in Tris-EDTA buffer) combined with DMSO and vehicle for the control group.

    Techniques: Permeability, Injection

    Cytokine and chemokine analyses of CP, hippocampus, and CSF after intracerebroventricular injection of Aβ1–42 oligomers. a – d , mRNA expression analysis of Il1 β ( a ), Il6 ( b ), Tnf ( c ), and Inos ( d ) in CP after intracerebroventricular

    Journal: The Journal of Neuroscience

    Article Title: Amyloid β Oligomers Disrupt Blood–CSF Barrier Integrity by Activating Matrix Metalloproteinases

    doi: 10.1523/JNEUROSCI.0006-15.2015

    Figure Lengend Snippet: Cytokine and chemokine analyses of CP, hippocampus, and CSF after intracerebroventricular injection of Aβ1–42 oligomers. a – d , mRNA expression analysis of Il1 β ( a ), Il6 ( b ), Tnf ( c ), and Inos ( d ) in CP after intracerebroventricular

    Article Snippet: To assess the role of MMPs, we used three treatments: Aβ1–42 oligomers (in Tris-EDTA buffer) combined with 1 μg of broad spectrum MMP inhibitor GM6001 (Merck; catalog #CC1100) dissolved in DMSO, Aβ1–42 oligomers (in Tris-EDTA buffer) combined with DMSO and vehicle for the control group.

    Techniques: Injection, Expressing

    CP morphology analysis by SFB-SEM. a , b , Representative SBF-SEM images of CP cells of C57BL/6 mice injected intracerebroventricularly with scrambled peptide ( a ) or Aβ1–42 oligomers ( b ). Cell shape is outlined in green. c , d , 3D modeling

    Journal: The Journal of Neuroscience

    Article Title: Amyloid β Oligomers Disrupt Blood–CSF Barrier Integrity by Activating Matrix Metalloproteinases

    doi: 10.1523/JNEUROSCI.0006-15.2015

    Figure Lengend Snippet: CP morphology analysis by SFB-SEM. a , b , Representative SBF-SEM images of CP cells of C57BL/6 mice injected intracerebroventricularly with scrambled peptide ( a ) or Aβ1–42 oligomers ( b ). Cell shape is outlined in green. c , d , 3D modeling

    Article Snippet: To assess the role of MMPs, we used three treatments: Aβ1–42 oligomers (in Tris-EDTA buffer) combined with 1 μg of broad spectrum MMP inhibitor GM6001 (Merck; catalog #CC1100) dissolved in DMSO, Aβ1–42 oligomers (in Tris-EDTA buffer) combined with DMSO and vehicle for the control group.

    Techniques: Mouse Assay, Injection

    CP morphology analysis by SFB-SEM of MMP3 −/− mice. a , b , Representative SFB-SEM images of the CPE cells of scrambled peptide ( a ) and Aβ1–42 oligomer ( b ) intracerebroventricularly injected MMP3 −/− mice. Cell

    Journal: The Journal of Neuroscience

    Article Title: Amyloid β Oligomers Disrupt Blood–CSF Barrier Integrity by Activating Matrix Metalloproteinases

    doi: 10.1523/JNEUROSCI.0006-15.2015

    Figure Lengend Snippet: CP morphology analysis by SFB-SEM of MMP3 −/− mice. a , b , Representative SFB-SEM images of the CPE cells of scrambled peptide ( a ) and Aβ1–42 oligomer ( b ) intracerebroventricularly injected MMP3 −/− mice. Cell

    Article Snippet: To assess the role of MMPs, we used three treatments: Aβ1–42 oligomers (in Tris-EDTA buffer) combined with 1 μg of broad spectrum MMP inhibitor GM6001 (Merck; catalog #CC1100) dissolved in DMSO, Aβ1–42 oligomers (in Tris-EDTA buffer) combined with DMSO and vehicle for the control group.

    Techniques: Mouse Assay, Injection

    Schematic overview of direct effects of Aβ1–42 oligomers on the BCSFB. A monolayer of CPE cells, tightly connected by tight junctions, restricts entrance of molecules from the fenestrated capillaries into the CSF. Injection of oligomerized

    Journal: The Journal of Neuroscience

    Article Title: Amyloid β Oligomers Disrupt Blood–CSF Barrier Integrity by Activating Matrix Metalloproteinases

    doi: 10.1523/JNEUROSCI.0006-15.2015

    Figure Lengend Snippet: Schematic overview of direct effects of Aβ1–42 oligomers on the BCSFB. A monolayer of CPE cells, tightly connected by tight junctions, restricts entrance of molecules from the fenestrated capillaries into the CSF. Injection of oligomerized

    Article Snippet: To assess the role of MMPs, we used three treatments: Aβ1–42 oligomers (in Tris-EDTA buffer) combined with 1 μg of broad spectrum MMP inhibitor GM6001 (Merck; catalog #CC1100) dissolved in DMSO, Aβ1–42 oligomers (in Tris-EDTA buffer) combined with DMSO and vehicle for the control group.

    Techniques: Injection

    Analysis of the role of MMPs in Aβ1–42 oligomer-induced disruption of BCSFB permeability. a , Fold change in Mmp gene expression in the CP 2 h (black) and 6 h (gray) after intracerebroventricular injection of Aβ1–42 oligomers

    Journal: The Journal of Neuroscience

    Article Title: Amyloid β Oligomers Disrupt Blood–CSF Barrier Integrity by Activating Matrix Metalloproteinases

    doi: 10.1523/JNEUROSCI.0006-15.2015

    Figure Lengend Snippet: Analysis of the role of MMPs in Aβ1–42 oligomer-induced disruption of BCSFB permeability. a , Fold change in Mmp gene expression in the CP 2 h (black) and 6 h (gray) after intracerebroventricular injection of Aβ1–42 oligomers

    Article Snippet: To assess the role of MMPs, we used three treatments: Aβ1–42 oligomers (in Tris-EDTA buffer) combined with 1 μg of broad spectrum MMP inhibitor GM6001 (Merck; catalog #CC1100) dissolved in DMSO, Aβ1–42 oligomers (in Tris-EDTA buffer) combined with DMSO and vehicle for the control group.

    Techniques: Permeability, Expressing, Injection

    Cleavage of SC pUC19 DNA (50 μ M) by platinum complex (100 μ M) in the presence of H 2 O 2 (100 μ M), in 10 mM Tris-HCl/1 mM EDTA buffer (pH 8.0). lane 1: DNA Marker; lane 2: DNA control; lane 3: DNA + complex; lane 4: DNA + complex 1 + H 2 O 2 ; lane 5: DNA + complex + H 2 O 2 + DMSO (4 μ L); lane 6: DNA + complex 1 + H 2 O 2 + NaN 3 (100 μ M).

    Journal: Bioinorganic Chemistry and Applications

    Article Title: DNA Interaction and DNA Cleavage Studies of a New Platinum(II) Complex Containing Aliphatic and Aromatic Dinitrogen Ligands

    doi: 10.1155/2011/525794

    Figure Lengend Snippet: Cleavage of SC pUC19 DNA (50 μ M) by platinum complex (100 μ M) in the presence of H 2 O 2 (100 μ M), in 10 mM Tris-HCl/1 mM EDTA buffer (pH 8.0). lane 1: DNA Marker; lane 2: DNA control; lane 3: DNA + complex; lane 4: DNA + complex 1 + H 2 O 2 ; lane 5: DNA + complex + H 2 O 2 + DMSO (4 μ L); lane 6: DNA + complex 1 + H 2 O 2 + NaN 3 (100 μ M).

    Article Snippet: Materials 4,7-diphenyl-1,10-phenanthroline (DIP), N,N -dimethyltrimethylenediamine, hydrazine dihydrochloride, potassium Chloride, nitric acid, Tris-acetate-EDTA (TAE) buffer, hydrogen peroxide, NaN3 , and Tris-HCl were purchased from Merck.

    Techniques: Marker