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Mediatech ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by Mediatech, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/Mediatech
Average 85 stars, based on 6 article reviews
Price from $9.99 to $1999.99
ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
85/100 stars

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Related Articles

Cell Isolation:

Article Title: Quantitative fluorescent profiling of VEGFRs reveals tumor cell and endothelial cell heterogeneity in breast cancer xenografts
Article Snippet: Paragraph title: Endothelial cell isolation ... Cells from an individual tumor are centrifuged at 300g for 5 min and resuspended in 30 mL of 0.2 μm filtered isolation buffer, containing PBS without calcium and magnesium (Invitrogen), 2 mmol/L EDTA (Mediatech), and 0.1% BSA (Sigma-Aldrich, St. Louis, MO).

Article Title: Injectable Hydrogel Scaffold from Decellularized Human Lipoaspirate
Article Snippet: Paragraph title: 2.1 Collection of source material and cell isolation ... When cells reached 80% confluence they were washed with 1x PBS and released from the tissue culture surface using 0.25% Trypsin/ 2.21 mM EDTA (Mediatech, Manassas, VA).

Cytometry:

Article Title: The coxsackievirus and adenovirus receptor acts as a tumour suppressor in malignant glioma cells
Article Snippet: .. Flow cytometry Confluent cells were released with Versene and resuspended in phosphate-buffered saline (PBS; Mediatech) containing 1% bovine serum albumin (BSA; Boehringer Mannheim, Indianapolis, IN, USA) at a concentration of 1–2 × 106 cells per ml. .. One hundred microliters of cells (1–2 × 105 ) were incubated with 100 μ l of anti-hCAR monoclonal antibody RmcB at a concentration of 10 μ g ml−1 for 1 h at 4°C with constant shaking.

Incubation:

Article Title: Micropost arrays for measuring stem cell-derived cardiomyocyte contractility
Article Snippet: .. After incubation at 37 °C for 3 minutes, the Versene is aspirated and replaced with a solution of 0.25% trypsin ethylenediaminetetra-acetic acid (Mediatech, Manassas, VA) in Versene. ..

Article Title: The coxsackievirus and adenovirus receptor acts as a tumour suppressor in malignant glioma cells
Article Snippet: Flow cytometry Confluent cells were released with Versene and resuspended in phosphate-buffered saline (PBS; Mediatech) containing 1% bovine serum albumin (BSA; Boehringer Mannheim, Indianapolis, IN, USA) at a concentration of 1–2 × 106 cells per ml. .. One hundred microliters of cells (1–2 × 105 ) were incubated with 100 μ l of anti-hCAR monoclonal antibody RmcB at a concentration of 10 μ g ml−1 for 1 h at 4°C with constant shaking.

Article Title: Microbial Amyloids Induce Interleukin 17A (IL-17A) and IL-22 Responses via Toll-Like Receptor 2 Activation in the Intestinal Mucosa
Article Snippet: Tissue sections were incubated at 37°C with shaking (100 rpm) for 20 min. To extract intraepithelial lymphocytes (IELs), the mixture was put through a 100-μm cell strainer. .. IEL-depleted tissue sections were then washed free of EDTA with ice-cold Ca2+ - and Mg2+ -free Hanks balanced salt solution (Mediatech, Inc.) with 2% FBS (Gibco) and 15 mM HEPES (Fisher Scientific).

Cell Culture:

Article Title: Ice Recovery Assay for Detection of Golgi-Derived Microtubules
Article Snippet: Paragraph title: 24.1.1 Cell culture and transfection ... Trypsin solution : 0.25% trypsin with EDTA, without sodium bicarbonate, calcium, and magnesium (Mediatech).

Article Title: Increased endogenous H2S generation by CBS, CSE, and 3MST gene therapy improves ex vivo renovascular relaxation in hyperhomocysteinemia
Article Snippet: .. T-25 flasks were grown in a tissue culture incubator, trypsinized (0.25% trypsin, 0.1% EDTA in HBSS without Ca2+ , Mg2+ , and sodium bicarbonate; Mediatech), and plated onto 12-well TPP (Techno Plastic Products, Trasadingen, Switzerland) cell culture plate. .. For the transfection study, cells were allowed to grow ∼50% confluence and then transfected with either CBS, CSE, 3MST, or triple genes.

Article Title: Micropost arrays for measuring stem cell-derived cardiomyocyte contractility
Article Snippet: Paragraph title: 2.4 Cell Culture and Preparation ... After incubation at 37 °C for 3 minutes, the Versene is aspirated and replaced with a solution of 0.25% trypsin ethylenediaminetetra-acetic acid (Mediatech, Manassas, VA) in Versene.

Article Title: Multiplexed Targeting of Barrett’s Neoplasia with a Heterobivalent Ligand: Imaging Study on Mouse Xenograft In Vivo and Human Specimens Ex Vivo
Article Snippet: Human OE33 and QhTERT esophageal cells were cultured with Roswell Park Memorial Institute (RPMI) 1640 media and keratinocyte-SFM media (Gibco), respectively. .. Cells were passaged using 0.25% EDTA containing trypsin (Mediatech), and a hemocytometer was used to count cell number.

Article Title: Injectable Hydrogel Scaffold from Decellularized Human Lipoaspirate
Article Snippet: Briefly, the tissue was digested in 0.075% collagenase I (Worthington Biochemical Corp., Lakewood, NJ) for 20 minutes and the resulting suspension was centrifuged at 5000 x g. The hASC-rich pellet was resuspended in 160 mM ammonium chloride to lyse blood cells and again centrifuged at 5000 x g. The remaining cells were filtered and resuspended in Growth Medium (Dulbecco’s modified essential medium / Ham’s F12 (DMEM/ F12, Mediatech, Manassas, VA), 10% fetal bovine serum (FBS, Gemini Bio-Products, Sacramento, CA), and 100 I.U. penicillin/ 100 μg/ mL streptomycin) and cultured overnight on standard tissue culture plastic at 37°C and 5% CO2 . .. When cells reached 80% confluence they were washed with 1x PBS and released from the tissue culture surface using 0.25% Trypsin/ 2.21 mM EDTA (Mediatech, Manassas, VA).

Expressing:

Article Title: Increased endogenous H2S generation by CBS, CSE, and 3MST gene therapy improves ex vivo renovascular relaxation in hyperhomocysteinemia
Article Snippet: T-25 flasks were grown in a tissue culture incubator, trypsinized (0.25% trypsin, 0.1% EDTA in HBSS without Ca2+ , Mg2+ , and sodium bicarbonate; Mediatech), and plated onto 12-well TPP (Techno Plastic Products, Trasadingen, Switzerland) cell culture plate. .. After 48 h of transfection, cells either were lysed to measured CBS, CSE, and 3MST protein expression and/or to detect H2 S generation capability or treated with or without Hcy (75 μM) to collect mitochondria.

Modification:

Article Title: Ice Recovery Assay for Detection of Golgi-Derived Microtubules
Article Snippet: Complete media : Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix (DMEM F-12K, Mediatech) supplemented with 10% fetal bovine serum (Atlanta Biologicals), and 1 mM L-glutamine (Mediatech). .. Trypsin solution : 0.25% trypsin with EDTA, without sodium bicarbonate, calcium, and magnesium (Mediatech).

Article Title: Injectable Hydrogel Scaffold from Decellularized Human Lipoaspirate
Article Snippet: Briefly, the tissue was digested in 0.075% collagenase I (Worthington Biochemical Corp., Lakewood, NJ) for 20 minutes and the resulting suspension was centrifuged at 5000 x g. The hASC-rich pellet was resuspended in 160 mM ammonium chloride to lyse blood cells and again centrifuged at 5000 x g. The remaining cells were filtered and resuspended in Growth Medium (Dulbecco’s modified essential medium / Ham’s F12 (DMEM/ F12, Mediatech, Manassas, VA), 10% fetal bovine serum (FBS, Gemini Bio-Products, Sacramento, CA), and 100 I.U. penicillin/ 100 μg/ mL streptomycin) and cultured overnight on standard tissue culture plastic at 37°C and 5% CO2 . .. When cells reached 80% confluence they were washed with 1x PBS and released from the tissue culture surface using 0.25% Trypsin/ 2.21 mM EDTA (Mediatech, Manassas, VA).

Western Blot:

Article Title: Interactions with Pocket Proteins Contribute to the Role of Human Papillomavirus Type 16 E7 in the Papillomavirus Life Cycle
Article Snippet: Paragraph title: Western analyses. ... Subsequently, cells were either directly lysed with the addition of lysis buffer onto the plates at 4°C or treated with 0.05% trypsin-0.53 mM EDTA (Mediatech, Inc., Herndon, VA).

Transfection:

Article Title: Ice Recovery Assay for Detection of Golgi-Derived Microtubules
Article Snippet: Paragraph title: 24.1.1 Cell culture and transfection ... Trypsin solution : 0.25% trypsin with EDTA, without sodium bicarbonate, calcium, and magnesium (Mediatech).

Article Title: Increased endogenous H2S generation by CBS, CSE, and 3MST gene therapy improves ex vivo renovascular relaxation in hyperhomocysteinemia
Article Snippet: T-25 flasks were grown in a tissue culture incubator, trypsinized (0.25% trypsin, 0.1% EDTA in HBSS without Ca2+ , Mg2+ , and sodium bicarbonate; Mediatech), and plated onto 12-well TPP (Techno Plastic Products, Trasadingen, Switzerland) cell culture plate. .. For the transfection study, cells were allowed to grow ∼50% confluence and then transfected with either CBS, CSE, 3MST, or triple genes.

Transferring:

Article Title: Micropost arrays for measuring stem cell-derived cardiomyocyte contractility
Article Snippet: After incubation at 37 °C for 3 minutes, the Versene is aspirated and replaced with a solution of 0.25% trypsin ethylenediaminetetra-acetic acid (Mediatech, Manassas, VA) in Versene. .. The cell suspension is repeatedly triturated by pipette to break up any large clumps and ensure the suspension contains individual cells and not clusters of cells.

Solubility:

Article Title: Photosensitizer (PS)-Cyanine Dye (CD) Conjugates: Impact of the Linkers Joining the PS and CD Moieties and Their Orientation in Tumor-Uptake and Photodynamic Therapy (PDT)
Article Snippet: All reagents, except FCS, but including Trypsin/EDTA, 1× (0.25 % Trypsin / 2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium, sterile, Porcine Parvovirus tested) and DPBS, 1× (Dulbecco’s Phosphate Buffered Saline), without calcium and magnesium, sterile were purchased from MediaTech, Inc., Manassas VA 20109. .. All compounds were formulated in 1 % Tween-80/D5W for solubility in aqueous solution and diluted in complete medium for all in vitro studies.

other:

Article Title: Lipoxin Signaling in Murine Lung Host Responses to Cryptococcus neoformans Infection
Article Snippet: After lavage with PBS containing 0.6 mM EDTA, lungs were perfused with PBS and transferred to ice-cold HBSS (Mediatech, Manassas, VA).

MTT Assay:

Article Title: Photosensitizer (PS)-Cyanine Dye (CD) Conjugates: Impact of the Linkers Joining the PS and CD Moieties and Their Orientation in Tumor-Uptake and Photodynamic Therapy (PDT)
Article Snippet: All reagents, except FCS, but including Trypsin/EDTA, 1× (0.25 % Trypsin / 2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium, sterile, Porcine Parvovirus tested) and DPBS, 1× (Dulbecco’s Phosphate Buffered Saline), without calcium and magnesium, sterile were purchased from MediaTech, Inc., Manassas VA 20109. .. The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) cell viability assays were read on a microtiter plate reader at an absorbance of 560 nm.

Fluorescence:

Article Title: Evaluation of Polymethine Dyes as Potential Probes for Near Infrared Fluorescence Imaging of Tumors: Part - 1
Article Snippet: Additional long-pass filters [a 950 LP filter and a 538 AELP filter (both from Omega Optical)] were used to attenuate the scattered light and fluorescence from the samples. .. All reagents, except FCS, but including Trypsin/EDTA, 1x (0.25 % Trypsin / 2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium, sterile, Porcine Parvovirus tested) and DPBS, 1x (Dulbecco's Phosphate Buffered Saline), without calcium and magnesium, sterile were purchased from MediaTech, Inc., Manassas VA 20109.

Isolation:

Article Title: Quantitative fluorescent profiling of VEGFRs reveals tumor cell and endothelial cell heterogeneity in breast cancer xenografts
Article Snippet: .. Cells from an individual tumor are centrifuged at 300g for 5 min and resuspended in 30 mL of 0.2 μm filtered isolation buffer, containing PBS without calcium and magnesium (Invitrogen), 2 mmol/L EDTA (Mediatech), and 0.1% BSA (Sigma-Aldrich, St. Louis, MO). .. Mouse tEC are isolated from the cell suspension using DSB-X (Invitrogen) biotinylated mouse CD31 antibody (eBioscience and BD Bioscience, San Diego, CA) and FlowComp Dynabeads (Invitrogen) according to the manufacturers' instructions.

Article Title: Injectable Hydrogel Scaffold from Decellularized Human Lipoaspirate
Article Snippet: Adipose-derived mesenchymal stem cells (hASCs) were first isolated from the tissue according to established protocols [ , ]. .. When cells reached 80% confluence they were washed with 1x PBS and released from the tissue culture surface using 0.25% Trypsin/ 2.21 mM EDTA (Mediatech, Manassas, VA).

Article Title: Microbial Amyloids Induce Interleukin 17A (IL-17A) and IL-22 Responses via Toll-Like Receptor 2 Activation in the Intestinal Mucosa
Article Snippet: Paragraph title: Isolation of cells from the cecum. ... IEL-depleted tissue sections were then washed free of EDTA with ice-cold Ca2+ - and Mg2+ -free Hanks balanced salt solution (Mediatech, Inc.) with 2% FBS (Gibco) and 15 mM HEPES (Fisher Scientific).

Article Title: Interactions with Pocket Proteins Contribute to the Role of Human Papillomavirus Type 16 E7 in the Papillomavirus Life Cycle
Article Snippet: Subsequently, cells were either directly lysed with the addition of lysis buffer onto the plates at 4°C or treated with 0.05% trypsin-0.53 mM EDTA (Mediatech, Inc., Herndon, VA). .. Cell pellets were then isolated by centrifugation at 1,100 rpm for 5 min at 4°C.

Flow Cytometry:

Article Title: The coxsackievirus and adenovirus receptor acts as a tumour suppressor in malignant glioma cells
Article Snippet: .. Flow cytometry Confluent cells were released with Versene and resuspended in phosphate-buffered saline (PBS; Mediatech) containing 1% bovine serum albumin (BSA; Boehringer Mannheim, Indianapolis, IN, USA) at a concentration of 1–2 × 106 cells per ml. .. One hundred microliters of cells (1–2 × 105 ) were incubated with 100 μ l of anti-hCAR monoclonal antibody RmcB at a concentration of 10 μ g ml−1 for 1 h at 4°C with constant shaking.

Mouse Assay:

Article Title: Quantitative fluorescent profiling of VEGFRs reveals tumor cell and endothelial cell heterogeneity in breast cancer xenografts
Article Snippet: Endothelial cell isolation Mice are euthanized with CO2 for ∼5 min, tumors are excised and placed in a 50 mL conical tube on ice containing cold Hanks balanced salt solution without magnesium and without chloride (Mediatech, Manassas, VA). .. Cells from an individual tumor are centrifuged at 300g for 5 min and resuspended in 30 mL of 0.2 μm filtered isolation buffer, containing PBS without calcium and magnesium (Invitrogen), 2 mmol/L EDTA (Mediatech), and 0.1% BSA (Sigma-Aldrich, St. Louis, MO).

Article Title: Evaluation of Polymethine Dyes as Potential Probes for Near Infrared Fluorescence Imaging of Tumors: Part - 1
Article Snippet: All reagents, except FCS, but including Trypsin/EDTA, 1x (0.25 % Trypsin / 2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium, sterile, Porcine Parvovirus tested) and DPBS, 1x (Dulbecco's Phosphate Buffered Saline), without calcium and magnesium, sterile were purchased from MediaTech, Inc., Manassas VA 20109. .. BALB/c mice 5-8 weeks of age were obtained from NCI Jackson Laboratory.

Article Title: Microbial Amyloids Induce Interleukin 17A (IL-17A) and IL-22 Responses via Toll-Like Receptor 2 Activation in the Intestinal Mucosa
Article Snippet: Ceca of mice were removed and cleared of feces with ice-cold Ca2+ - and Mg2+ -free Hanks balanced salt solution (Mediatech, Inc.) with 2% fetal bovine serum (FBS) (Gibco) and 15 mM HEPES (Fisher Scientific). .. IEL-depleted tissue sections were then washed free of EDTA with ice-cold Ca2+ - and Mg2+ -free Hanks balanced salt solution (Mediatech, Inc.) with 2% FBS (Gibco) and 15 mM HEPES (Fisher Scientific).

Centrifugation:

Article Title: Interactions with Pocket Proteins Contribute to the Role of Human Papillomavirus Type 16 E7 in the Papillomavirus Life Cycle
Article Snippet: Subsequently, cells were either directly lysed with the addition of lysis buffer onto the plates at 4°C or treated with 0.05% trypsin-0.53 mM EDTA (Mediatech, Inc., Herndon, VA). .. Cell pellets were then isolated by centrifugation at 1,100 rpm for 5 min at 4°C.

Plasmid Preparation:

Article Title: Ice Recovery Assay for Detection of Golgi-Derived Microtubules
Article Snippet: Trypsin solution : 0.25% trypsin with EDTA, without sodium bicarbonate, calcium, and magnesium (Mediatech). .. Plasmid : Centrosome marker (e.g., Centrin2-EGFP, Supplied by Jeffrey Salisbury on Addgene).

Article Title: Increased endogenous H2S generation by CBS, CSE, and 3MST gene therapy improves ex vivo renovascular relaxation in hyperhomocysteinemia
Article Snippet: T-25 flasks were grown in a tissue culture incubator, trypsinized (0.25% trypsin, 0.1% EDTA in HBSS without Ca2+ , Mg2+ , and sodium bicarbonate; Mediatech), and plated onto 12-well TPP (Techno Plastic Products, Trasadingen, Switzerland) cell culture plate. .. Transfection efficacy was measured by GFP vector in separate experiments.

In Vitro:

Article Title: Photosensitizer (PS)-Cyanine Dye (CD) Conjugates: Impact of the Linkers Joining the PS and CD Moieties and Their Orientation in Tumor-Uptake and Photodynamic Therapy (PDT)
Article Snippet: Paragraph title: In vitro tumor models ... All reagents, except FCS, but including Trypsin/EDTA, 1× (0.25 % Trypsin / 2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium, sterile, Porcine Parvovirus tested) and DPBS, 1× (Dulbecco’s Phosphate Buffered Saline), without calcium and magnesium, sterile were purchased from MediaTech, Inc., Manassas VA 20109.

Article Title: Evaluation of Polymethine Dyes as Potential Probes for Near Infrared Fluorescence Imaging of Tumors: Part - 1
Article Snippet: In vitro tumor models: Colon 26 cells were grown in sterile RPMI-1640, 1x with L-glutamine with 10 % Fetal Calf Serum (FCS) (Atlanta Biologicals, triple 0.1µm filtered, Lawrenceville, GA), and 1 % Penicillin/Steptomycin/L-glutamine (P/S/l -G 10,000 I.U/ml penicillin, 10,000 μg/ml streptomycin, 29.2 mg/ml L-glutamine) was maintained in 5 % CO2 , 95 % air and 100 % humidity. .. All reagents, except FCS, but including Trypsin/EDTA, 1x (0.25 % Trypsin / 2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium, sterile, Porcine Parvovirus tested) and DPBS, 1x (Dulbecco's Phosphate Buffered Saline), without calcium and magnesium, sterile were purchased from MediaTech, Inc., Manassas VA 20109.

Homogenization:

Article Title: Microbial Amyloids Induce Interleukin 17A (IL-17A) and IL-22 Responses via Toll-Like Receptor 2 Activation in the Intestinal Mucosa
Article Snippet: IEL-depleted tissue sections were then washed free of EDTA with ice-cold Ca2+ - and Mg2+ -free Hanks balanced salt solution (Mediatech, Inc.) with 2% FBS (Gibco) and 15 mM HEPES (Fisher Scientific). .. Digested tissue was subjected to homogenization with a gentleMACS dissociator (Miltenyi Biotech).

Concentration Assay:

Article Title: The coxsackievirus and adenovirus receptor acts as a tumour suppressor in malignant glioma cells
Article Snippet: .. Flow cytometry Confluent cells were released with Versene and resuspended in phosphate-buffered saline (PBS; Mediatech) containing 1% bovine serum albumin (BSA; Boehringer Mannheim, Indianapolis, IN, USA) at a concentration of 1–2 × 106 cells per ml. .. One hundred microliters of cells (1–2 × 105 ) were incubated with 100 μ l of anti-hCAR monoclonal antibody RmcB at a concentration of 10 μ g ml−1 for 1 h at 4°C with constant shaking.

Marker:

Article Title: Ice Recovery Assay for Detection of Golgi-Derived Microtubules
Article Snippet: Trypsin solution : 0.25% trypsin with EDTA, without sodium bicarbonate, calcium, and magnesium (Mediatech). .. Plasmid : Centrosome marker (e.g., Centrin2-EGFP, Supplied by Jeffrey Salisbury on Addgene).

Lysis:

Article Title: Interactions with Pocket Proteins Contribute to the Role of Human Papillomavirus Type 16 E7 in the Papillomavirus Life Cycle
Article Snippet: .. Subsequently, cells were either directly lysed with the addition of lysis buffer onto the plates at 4°C or treated with 0.05% trypsin-0.53 mM EDTA (Mediatech, Inc., Herndon, VA). ..

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  • 89
    Mediatech versene
    In vitro soft agar colony-formation assay. U-118 MG cells were transfected with the various hCAR expression vectors and stable transfectants selected in the presence of 400 μ g ml −1 G418. Monolayers of cells were released with <t>Versene</t> and dispersed into a suspension of single cells in growth medium. One thousand cells were resuspended in 2 ml of growth medium containing 0.4% low melting temperature agarose (and 400 μ g ml −1 G418 in the case of the transfectants), and were overlaid in triplicate on 2 ml of solidified 0.8% low melting temperature agarose in growth medium in a 6-well dish. The dishes were incubated for 18 days at 37°C in a 5% CO 2 atmosphere. Colonies larger than 50 cells were then counted. The mean colony sizes±standard deviations are shown. Statistical analysis was performed by one-way ANOVA. P
    Versene, supplied by Mediatech, used in various techniques. Bioz Stars score: 89/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/versene/product/Mediatech
    Average 89 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    versene - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

    95
    Mediatech trypsin edta
    In vitro soft agar colony-formation assay. U-118 MG cells were transfected with the various hCAR expression vectors and stable transfectants selected in the presence of 400 μ g ml −1 G418. Monolayers of cells were released with <t>Versene</t> and dispersed into a suspension of single cells in growth medium. One thousand cells were resuspended in 2 ml of growth medium containing 0.4% low melting temperature agarose (and 400 μ g ml −1 G418 in the case of the transfectants), and were overlaid in triplicate on 2 ml of solidified 0.8% low melting temperature agarose in growth medium in a 6-well dish. The dishes were incubated for 18 days at 37°C in a 5% CO 2 atmosphere. Colonies larger than 50 cells were then counted. The mean colony sizes±standard deviations are shown. Statistical analysis was performed by one-way ANOVA. P
    Trypsin Edta, supplied by Mediatech, used in various techniques. Bioz Stars score: 95/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta/product/Mediatech
    Average 95 stars, based on 92 article reviews
    Price from $9.99 to $1999.99
    trypsin edta - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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    In vitro soft agar colony-formation assay. U-118 MG cells were transfected with the various hCAR expression vectors and stable transfectants selected in the presence of 400 μ g ml −1 G418. Monolayers of cells were released with Versene and dispersed into a suspension of single cells in growth medium. One thousand cells were resuspended in 2 ml of growth medium containing 0.4% low melting temperature agarose (and 400 μ g ml −1 G418 in the case of the transfectants), and were overlaid in triplicate on 2 ml of solidified 0.8% low melting temperature agarose in growth medium in a 6-well dish. The dishes were incubated for 18 days at 37°C in a 5% CO 2 atmosphere. Colonies larger than 50 cells were then counted. The mean colony sizes±standard deviations are shown. Statistical analysis was performed by one-way ANOVA. P

    Journal: British Journal of Cancer

    Article Title: The coxsackievirus and adenovirus receptor acts as a tumour suppressor in malignant glioma cells

    doi: 10.1038/sj.bjc.6600932

    Figure Lengend Snippet: In vitro soft agar colony-formation assay. U-118 MG cells were transfected with the various hCAR expression vectors and stable transfectants selected in the presence of 400 μ g ml −1 G418. Monolayers of cells were released with Versene and dispersed into a suspension of single cells in growth medium. One thousand cells were resuspended in 2 ml of growth medium containing 0.4% low melting temperature agarose (and 400 μ g ml −1 G418 in the case of the transfectants), and were overlaid in triplicate on 2 ml of solidified 0.8% low melting temperature agarose in growth medium in a 6-well dish. The dishes were incubated for 18 days at 37°C in a 5% CO 2 atmosphere. Colonies larger than 50 cells were then counted. The mean colony sizes±standard deviations are shown. Statistical analysis was performed by one-way ANOVA. P

    Article Snippet: Flow cytometry Confluent cells were released with Versene and resuspended in phosphate-buffered saline (PBS; Mediatech) containing 1% bovine serum albumin (BSA; Boehringer Mannheim, Indianapolis, IN, USA) at a concentration of 1–2 × 106 cells per ml.

    Techniques: In Vitro, Soft Agar Assay, Transfection, Expressing, Incubation