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HiMedia Laboratories ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic Acid, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacetic acid/product/HiMedia Laboratories
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ethylenediaminetetraacetic acid - by Bioz Stars, 2020-04
93/100 stars

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DNA Extraction:

Article Title: A Simple Method of Genomic DNA Extraction from Human Samples for PCR-RFLP Analysis
Article Snippet: Paragraph title: DNA Extraction from Buccal Swabs ... The buccal swab samples were suspended in 500 μl lysis buffer [10 mM Tris (pH 8.0), 10 mM EDTA, and 2.0% SDS], and 50 μl 10% SDS, followed by 5–10 μl 20 mg/ml proteinase K (Himedia, Mumbai, India), was added.

MTT Assay:

Article Title: Effect of liposomal celecoxib on proliferation of colon cancer cell and inhibition of DMBA-induced tumor in rat model
Article Snippet: Materials and methods DSPC (1,2-distearoyl-sn -glycero-phosphatidylcholine), MTT (3-(4,5-dimethyltiazol-2-yl)-2,5-diphenyltetrazolium bromide), PI (propidium iodide), DAPI (4′,6-diamidino-2-phenylindole), reduced glutathione, DTNB (5,5-dithiobis(2-nitrobenzoic acid)), RNase (ribonuclease), and DMBA were obtained from Sigma Aldrich, USA. .. Phosphotungstic acid, thiobarbituric acid, trichloroacetic acid, acetic acid, sodium salicylate, and EDTA were purchased from the Himedia Chemicals (Pvt) Ltd, Mumbai, India.

Centrifugation:

Article Title: Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta
Article Snippet: Their solid fractions were washed with 1ml of 0.03M NaCl-0.002M EDTA (pH: 8) by centrifugation at 12,000 rpm/5 min, 4°C. .. After three washing cycles, they were suspended in 100 µl of 0.03M NaCl-0.002M EDTA (pH: 8) and 100 µl freshly prepared lysozyme (10 mg/ml, HiMedia, India).

Article Title: HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis
Article Snippet: Undigested glands were then treated with 0.25 % trypsin-0.02 % EDTA (Himedia, Mumbai, India) for 4–8 min, washed with 10 % FBS-DMEM. .. Single epithelial cells were isolated by centrifugation, as described previously.

Positron Emission Tomography:

Article Title: N-terminal Proline-rich Domain Is Required for Scrambling Activity of Human Phospholipid Scramblases *
Article Snippet: Escherichia coli DH5α and BL21 (DE3) strains were obtained from ATCC. cDNA of hPLSCR1 and -2 was purchased from Invitrogen, and pET-28b(+) was from Novagen. .. Isopropyl β- d -1-thiogalactopyranoside, dithiothreitol (DTT), and EDTA were purchased from Himedia.

Isolation:

Article Title: HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis
Article Snippet: Paragraph title: Isolation of cells and flow cytometric analysis ... Undigested glands were then treated with 0.25 % trypsin-0.02 % EDTA (Himedia, Mumbai, India) for 4–8 min, washed with 10 % FBS-DMEM.

Article Title: Titanium dioxide nanoparticles: an in vitro study of DNA binding, chromosome aberration assay, and comet assay
Article Snippet: Complete growth medium; RPMI-1640 HiKaryoXL™ -AL165A; low melting point agarose, agarose, sodium chloride, sodium EDTA, Trizma base, 1% Triton X-100, DMSO, EDTA and Ethidium bromide, were purchased from Himedia (Mumbai, India). .. Human genomic DNA was isolated from normal WBCs of peripheral blood as per manufacturer’s protocol Qiagen’s kit (Cat. no. 51104) (New Delhi, India).

Flow Cytometry:

Article Title: HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis
Article Snippet: Paragraph title: Isolation of cells and flow cytometric analysis ... Undigested glands were then treated with 0.25 % trypsin-0.02 % EDTA (Himedia, Mumbai, India) for 4–8 min, washed with 10 % FBS-DMEM.

Cell Culture:

Article Title: Prevalence of Carbapenem Resistant Non-Fermenting Gram Negative Bacterial Infection and Identification of Carbapenemase Producing NFGNB Isolates by Simple Phenotypic Tests
Article Snippet: Combined Disc Test (CDT): The test isolates along with standard control strains (opacity adjusted to 0.5 McFarland opacity standard [ ] were lawn cultured on MHA plate as recommended by CLSI. .. A 10 μl of 0.5 M EDTA (Himedia) was added to one of the meropenem discs and incubated overnight.

Article Title: Selective Estrogen Receptor Modulator and prostimulatory effects of phytoestrogen β-ecdysone in Tinospora cordifolia on osteoblast cells
Article Snippet: The cells were cultured in ready to use sterile liquid Dulbecco's minimum essential medium-Eagle (DMEM AL007S, Himedia, India) supplemented with 1X antibiotic antimycotic solution (A007, Himedia, India) and 10% fetal bovine serum (FBS-RM1112, Himedia, India). .. When a confluent monolayer was formed, cells were detached with 0.25% trypsin–0.2% EDTA in Dulbecco's phosphate buffered saline (T-001, Himedia, India) and then subcultured at a split ratio of 1:3 in 12.5 cm2 volume tissue culture flask (TCG2 – Himedia, India).

Concentration Assay:

Article Title: Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta
Article Snippet: They were suspended in 100µl 0.03M NaCl-0.002M EDTA (pH: 8) and a variable concentration of freshly prepared lysozyme (15 mg/ml, 20 mg/ml, and 25 mg/ml, HiMedia, India) followed by thorough mixing and incubation at 37°C for 1 h. Remaining steps were carried out as mentioned above for method EM1. .. After three washing cycles, they were suspended in 100 µl of 0.03M NaCl-0.002M EDTA (pH: 8) and 100 µl freshly prepared lysozyme (10 mg/ml, HiMedia, India).

Incubation:

Article Title: Prevalence of Carbapenem Resistant Non-Fermenting Gram Negative Bacterial Infection and Identification of Carbapenemase Producing NFGNB Isolates by Simple Phenotypic Tests
Article Snippet: .. A 10 μl of 0.5 M EDTA (Himedia) was added to one of the meropenem discs and incubated overnight. .. Isolates showing ≥7 mm increase in the inhibition zone size of meropenem-EDTA disc than the meropenem disc alone were considered as MBL producers P [ ] [ ].

Article Title: A Simple Method of Genomic DNA Extraction from Human Samples for PCR-RFLP Analysis
Article Snippet: The buccal swab samples were suspended in 500 μl lysis buffer [10 mM Tris (pH 8.0), 10 mM EDTA, and 2.0% SDS], and 50 μl 10% SDS, followed by 5–10 μl 20 mg/ml proteinase K (Himedia, Mumbai, India), was added. .. RNase A (10 μl of 10 mg/ml; Fermentas, Thermo Scientific, Germany) was added, and the solution was incubated at 37°C for 30 min.

Article Title: Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta
Article Snippet: They were suspended in 100µl 0.03M NaCl-0.002M EDTA (pH: 8) and a variable concentration of freshly prepared lysozyme (15 mg/ml, 20 mg/ml, and 25 mg/ml, HiMedia, India) followed by thorough mixing and incubation at 37°C for 1 h. Remaining steps were carried out as mentioned above for method EM1. .. After three washing cycles, they were suspended in 100 µl of 0.03M NaCl-0.002M EDTA (pH: 8) and 100 µl freshly prepared lysozyme (10 mg/ml, HiMedia, India).

other:

Article Title: Activation of Human Salivary Aldehyde Dehydrogenase by Sulforaphane: Mechanism and Significance
Article Snippet: EDTA was the product of Himedia chemicals, India.

Article Title: Role of salicylic acid in induction of plant defense system in chickpea (Cicer arietinum L.)
Article Snippet: 2-mercaptoethanol (RM2895), Tris-hydrochloride (RM613), Polyvinylpyrolidone (PVP: RM854), EDTA (RM1279), Disodium hydrogen phosphate (RM1154), Sodium dihydrogen phosphate (RM1155), Guaiacol (RM1118), Gallic acid (RM233), Salicylic acid (RM1476) was obtained from HiMedia.

Article Title: In Vivo Assessment of Antihyperglycemic and Antioxidant Activity from Oil of Seeds of Brassica Nigra in Streptozotocin Induced Diabetic Rats
Article Snippet: The drugs and chemicals used in the study were glibenclamide (Torrent Pharmaceutical, Ahmadabad), streptozotocin, heparin (SRL, India), EDTA (Hi-media Lab.

BIA-KA:

Article Title: N-terminal Proline-rich Domain Is Required for Scrambling Activity of Human Phospholipid Scramblases *
Article Snippet: Isopropyl β- d -1-thiogalactopyranoside, dithiothreitol (DTT), and EDTA were purchased from Himedia. .. N -Lauroylsarcosine, BCA protein estimation kit, molecular biology grade CaCl2 , trypsin, and egg phosphatidylcholine, phosphatidylserine, and phenylmethylsulfonyl fluoride were purchased from Sigma.

Staining:

Article Title: Evaluation of GABAergic Transmission Modulation as a Novel Functional Target for Management of Multiple Sclerosis: Exploring Inhibitory Effect of GABA on Glutamate-Mediated Excitotoxicity
Article Snippet: .. Paraformaldehyde, percoll, sucrose, disodium EDTA, Tris buffer, HEPES sodium salt, sodium bicarbonate, calcium chloride, magnesium chloride, 4-aminopyridine, glutamate, NAD+ (oxidized form), cresyl violet acetate stain, and luxol fast blue stain were purchased from Himedia Laboratories Pvt. ..

Article Title: HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis
Article Snippet: Undigested glands were then treated with 0.25 % trypsin-0.02 % EDTA (Himedia, Mumbai, India) for 4–8 min, washed with 10 % FBS-DMEM. .. Single cell suspension thus obtained was divided into three parts; two parts were stained with mouse anti-human αv β3 integrin and LIF (R & D Systems, Minneapolis, MN, USA) according to instructions provided by the manufacturer in the manual.

Lysis:

Article Title: A Simple Method of Genomic DNA Extraction from Human Samples for PCR-RFLP Analysis
Article Snippet: .. The buccal swab samples were suspended in 500 μl lysis buffer [10 mM Tris (pH 8.0), 10 mM EDTA, and 2.0% SDS], and 50 μl 10% SDS, followed by 5–10 μl 20 mg/ml proteinase K (Himedia, Mumbai, India), was added. ..

Article Title: HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis
Article Snippet: Undigested glands were then treated with 0.25 % trypsin-0.02 % EDTA (Himedia, Mumbai, India) for 4–8 min, washed with 10 % FBS-DMEM. .. Isolated cells were washed, RBC lysed using RBC lysis solution and fixed in 2 % paraformaldehyde (20 min at RT).

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