ethylenediaminetetraacetate edta  (Millipore)


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    Structured Review

    Millipore ethylenediaminetetraacetate edta
    Ethylenediaminetetraacetate Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacetate edta/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetate edta - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Silver Nanoclusters with Specific Ion Recognition Modulated by Ligand Passivation toward Fluorimetric and Colorimetric Copper Analysis and Biological Imaging
    Article Snippet: .. Materials and Instruments Glutathione (GSH), α-lipoic acid (LA), silver nitrate (AgNO3 ), sodium hydroxide, sodium borohydride, copper nitrate (Cu (NO3 )2 ), sodium chloride, ethylenediaminetetraacetate (EDTA), yeast cells, thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (Beijing, China). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Up-Regulation of Phosphorylated/Activated p70 S6 Kinase and Its Relationship to Neurofibrillary Pathology in Alzheimer's Disease
    Article Snippet: Paragraph title: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) ... Microtiter plates (Nunc-Immuno, Roskilde, Denmark) were coated in duplicates with 2 μg of protein/well in 20 mmol/L Tris-HCl, pH 7.4, buffered with 2.5 mmol/L ethylenediaminetetraacetate (EDTA), 2.5 mmol/L EGTA, 0.1% (v/v) protease-inhibitor cocktail (Sigma-Aldrich, Stockholm, Sweden), 0.1% sodium azide, overnight at 4°C.

    Incubation:

    Article Title: Up-Regulation of Phosphorylated/Activated p70 S6 Kinase and Its Relationship to Neurofibrillary Pathology in Alzheimer's Disease
    Article Snippet: Microtiter plates (Nunc-Immuno, Roskilde, Denmark) were coated in duplicates with 2 μg of protein/well in 20 mmol/L Tris-HCl, pH 7.4, buffered with 2.5 mmol/L ethylenediaminetetraacetate (EDTA), 2.5 mmol/L EGTA, 0.1% (v/v) protease-inhibitor cocktail (Sigma-Aldrich, Stockholm, Sweden), 0.1% sodium azide, overnight at 4°C. .. The plates were incubated with rabbit antibodies to total p70 S6 kinase and p70 S6 kinase phosphorylated at T389 or T421/T424 (1:200), to total tau (R134, 1:2500), and to rpS6 (total) and S6 phosphorylated at S235/236 or S240/244 (1:250), or with mAbs to tau unphosphorylated at S198/199/202/T205 (Tau-1, 1:40,000), tau phosphorylated at S202/T205 (AT8, 1:500) and at S396/404 (PHF-1, 1:200) overnight at 4°C.

    Article Title: Defects in ciliary localization of Nek8 is associated with cystogenesis
    Article Snippet: Transfected IMCD-3 cells were resuspended in 1x sodium dodecyl sulfate (SDS) sample buffer [10% glycerol, 50 mM Tris (pH 6.8), 2 mM ethylenediaminetetraacetate (EDTA), 2% SDS, 144 mM 2-mercaptoethanol, 0.01% bromophenol blue (all from Sigma)], and the suspension was sonicated to shear genomic DNA. .. The nitrocellulose membrane was blocked using 5% skim milk in Tris-buffered saline (TBS)-T for 1 h, followed by incubation in mouse anti-GFP antibody (clones 7.1 and 13.1, 0.4 μg/mL, Roche) overnight at 4°C.

    Modification:

    Article Title: Type I Collagen as an Extracellular Matrix for the In Vitro Growth of Human Small Intestinal Epithelium
    Article Snippet: .. Crypts were isolated from the mucosa as previously described with slight modification ; 8 mM ethylenediaminetetraacetate (EDTA) (Sigma) and 1 mM dithiothreitol (DTT) (Sigma) were used for the dissociation step, and crypts were only filtered through a 100 µm pore cell strainer (BD Biosciences, Bedford MA). .. Crypts were resuspended in 5 mL of Basic Crypt Medium, consisting of Advanced DMEM/Ham's F12 (Invitrogen) with 2 mM GlutaMAX (Invitrogen), 10 mM HEPES (Invitrogen), and 1x Antibiotic-Antimycotic (Invitrogen).

    Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors
    Article Snippet: Cells and cell culture HEK293T cells (Invitrogen, Carlsbad, CA) and the HeLa cell line TZM-bl (Obtained through NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH, from John C Kappes, Xiaoyun Wu and Tranzyme (Catalogue No. 8129)) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Waltham, MA) supplemented with 10% fetal calf serum (FCS) (Gibco). .. Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C.

    Western Blot:

    Article Title: Defects in ciliary localization of Nek8 is associated with cystogenesis
    Article Snippet: Paragraph title: Western blot ... Transfected IMCD-3 cells were resuspended in 1x sodium dodecyl sulfate (SDS) sample buffer [10% glycerol, 50 mM Tris (pH 6.8), 2 mM ethylenediaminetetraacetate (EDTA), 2% SDS, 144 mM 2-mercaptoethanol, 0.01% bromophenol blue (all from Sigma)], and the suspension was sonicated to shear genomic DNA.

    Article Title: A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana
    Article Snippet: Paragraph title: Protein extraction and Western blot ... And then protein extraction buffer (1 mL) containing 50 mM 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES), 150 mM KCl, 1 mM ethylenediaminetetraacetate (EDTA) (pH 8.0), 0.1% triton X-100, 1 mM dl-dithiothreitol (DTT), and 1 × Protease Inhibitor Cocktail (Sigma) was added to 500 mg of each ground sample.

    Transfection:

    Article Title: Defects in ciliary localization of Nek8 is associated with cystogenesis
    Article Snippet: .. Transfected IMCD-3 cells were resuspended in 1x sodium dodecyl sulfate (SDS) sample buffer [10% glycerol, 50 mM Tris (pH 6.8), 2 mM ethylenediaminetetraacetate (EDTA), 2% SDS, 144 mM 2-mercaptoethanol, 0.01% bromophenol blue (all from Sigma)], and the suspension was sonicated to shear genomic DNA. .. The samples were separated by electrophoresis in an 8% SDS-polyacrylamide gel electrophoresis (PAGE) gel and transferred to Trans-Blot membrane (BioRad) using a wet transfer apparatus.

    Protease Inhibitor:

    Article Title: Up-Regulation of Phosphorylated/Activated p70 S6 Kinase and Its Relationship to Neurofibrillary Pathology in Alzheimer's Disease
    Article Snippet: .. Microtiter plates (Nunc-Immuno, Roskilde, Denmark) were coated in duplicates with 2 μg of protein/well in 20 mmol/L Tris-HCl, pH 7.4, buffered with 2.5 mmol/L ethylenediaminetetraacetate (EDTA), 2.5 mmol/L EGTA, 0.1% (v/v) protease-inhibitor cocktail (Sigma-Aldrich, Stockholm, Sweden), 0.1% sodium azide, overnight at 4°C. .. Unspecific protein-binding sites were blocked with 3% BSA in 10 mmol/L Tris-HCl buffer with 0.85% NaCl and 0.2% Tween-20 for 1 hour at room temperature.

    Article Title: A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana
    Article Snippet: .. And then protein extraction buffer (1 mL) containing 50 mM 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES), 150 mM KCl, 1 mM ethylenediaminetetraacetate (EDTA) (pH 8.0), 0.1% triton X-100, 1 mM dl-dithiothreitol (DTT), and 1 × Protease Inhibitor Cocktail (Sigma) was added to 500 mg of each ground sample. .. Supernatants were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose blotting membrane.

    Cell Culture:

    Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors
    Article Snippet: Paragraph title: Cells and cell culture ... Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C.

    Article Title: In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76
    Article Snippet: The cells were cultured in minimal essential medium (MEM) (Sigma-Aldrich Inc., Tokyo, Japan) containing 10% fetal bovine serum (FBS) (Nichirei Biosciences Inc., Tokyo, Japan), 100 mg/ml streptomycin and 100 U/ml penicillin (Sigma-Aldrich). .. For subcultures, cells were rinsed with Ca2+ and Mg2+ -free phosphate buffered saline (PBS) (Sigma-Aldrich), and were dispersed with 0.25% trypsin containing 0.5 mM of ethylenediaminetetraacetate (EDTA) (Sigma-Aldrich).

    Light Microscopy:

    Article Title: Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages
    Article Snippet: .. The specimens were decalcified in 22.5% ethylenediaminetetraacetate (EDTA) and embedded in paraffin, and serial frontal sections of 7 μ m in thickness were prepared, stained with ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit (Millipore, Billerica, MA), and observed by light microscopy. ..

    Generated:

    Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors
    Article Snippet: Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C. .. MDDCs were generated by culturing purified monocytes in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% fetal calf serum (FCS), IL-4 (1,000 U/ml) and GM-CSF (1,000 U/ml) (Biosource, Carlsbad, CA) for 24 hours.

    other:

    Article Title: Expression of Calcitonin Gene-related Peptide in Rat Pulp and Periodontal Tissues by Indirect Immunofluorescence Method
    Article Snippet: The jaws were excised and post-fixed for 1 day in the same fixative as was used for perfusion, and thereafter demineralized for 4 weeks at 4°C in ethylenediaminetetraacetate (EDTA), containing 7.5% polyvinylpyrrolidone (PVP; Aldrich Chemics, Steinheim, Germany). After demineralization the jaws were immersed in 30% sucrose in 0.1 M phosphate buffer (pH 7.4) and serially sectioned sagittal at 30 μm on a freezing microtome.

    Article Title: Antioxidant activity and mechanism of commercial Rama Forte persimmon fruits (Diospyros kaki)
    Article Snippet: Ascorbic acid, 2-deoxyribose (2-DR), ferrous ammonium sulfate, N-[2-hydroxyethyl]-piperazine-N′-[2-ethanesulfonic acid] (HEPES), thiobarbituric acid (TBA), mono and dibasic potassium phosphate, ethylenediaminetetraacetate (EDTA), tannic acid, and 5,5-dimethyl-1-pirrolidine-N-oxide (DMPO) were purchased from Sigma (St. Louis, MO, USA).

    Sonication:

    Article Title: Defects in ciliary localization of Nek8 is associated with cystogenesis
    Article Snippet: .. Transfected IMCD-3 cells were resuspended in 1x sodium dodecyl sulfate (SDS) sample buffer [10% glycerol, 50 mM Tris (pH 6.8), 2 mM ethylenediaminetetraacetate (EDTA), 2% SDS, 144 mM 2-mercaptoethanol, 0.01% bromophenol blue (all from Sigma)], and the suspension was sonicated to shear genomic DNA. .. The samples were separated by electrophoresis in an 8% SDS-polyacrylamide gel electrophoresis (PAGE) gel and transferred to Trans-Blot membrane (BioRad) using a wet transfer apparatus.

    Nucleic Acid Electrophoresis:

    Article Title: Defects in ciliary localization of Nek8 is associated with cystogenesis
    Article Snippet: Transfected IMCD-3 cells were resuspended in 1x sodium dodecyl sulfate (SDS) sample buffer [10% glycerol, 50 mM Tris (pH 6.8), 2 mM ethylenediaminetetraacetate (EDTA), 2% SDS, 144 mM 2-mercaptoethanol, 0.01% bromophenol blue (all from Sigma)], and the suspension was sonicated to shear genomic DNA. .. The samples were separated by electrophoresis in an 8% SDS-polyacrylamide gel electrophoresis (PAGE) gel and transferred to Trans-Blot membrane (BioRad) using a wet transfer apparatus.

    Article Title: A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana
    Article Snippet: And then protein extraction buffer (1 mL) containing 50 mM 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES), 150 mM KCl, 1 mM ethylenediaminetetraacetate (EDTA) (pH 8.0), 0.1% triton X-100, 1 mM dl-dithiothreitol (DTT), and 1 × Protease Inhibitor Cocktail (Sigma) was added to 500 mg of each ground sample. .. Supernatants were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose blotting membrane.

    Magnetic Beads:

    Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors
    Article Snippet: .. Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C. .. MDDCs were generated by culturing purified monocytes in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% fetal calf serum (FCS), IL-4 (1,000 U/ml) and GM-CSF (1,000 U/ml) (Biosource, Carlsbad, CA) for 24 hours.

    Isolation:

    Article Title: Type I Collagen as an Extracellular Matrix for the In Vitro Growth of Human Small Intestinal Epithelium
    Article Snippet: .. Crypts were isolated from the mucosa as previously described with slight modification ; 8 mM ethylenediaminetetraacetate (EDTA) (Sigma) and 1 mM dithiothreitol (DTT) (Sigma) were used for the dissociation step, and crypts were only filtered through a 100 µm pore cell strainer (BD Biosciences, Bedford MA). .. Crypts were resuspended in 5 mL of Basic Crypt Medium, consisting of Advanced DMEM/Ham's F12 (Invitrogen) with 2 mM GlutaMAX (Invitrogen), 10 mM HEPES (Invitrogen), and 1x Antibiotic-Antimycotic (Invitrogen).

    Purification:

    Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors
    Article Snippet: .. Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C. .. MDDCs were generated by culturing purified monocytes in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% fetal calf serum (FCS), IL-4 (1,000 U/ml) and GM-CSF (1,000 U/ml) (Biosource, Carlsbad, CA) for 24 hours.

    Indirect ELISA:

    Article Title: Up-Regulation of Phosphorylated/Activated p70 S6 Kinase and Its Relationship to Neurofibrillary Pathology in Alzheimer's Disease
    Article Snippet: Paragraph title: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) ... Microtiter plates (Nunc-Immuno, Roskilde, Denmark) were coated in duplicates with 2 μg of protein/well in 20 mmol/L Tris-HCl, pH 7.4, buffered with 2.5 mmol/L ethylenediaminetetraacetate (EDTA), 2.5 mmol/L EGTA, 0.1% (v/v) protease-inhibitor cocktail (Sigma-Aldrich, Stockholm, Sweden), 0.1% sodium azide, overnight at 4°C.

    Protein Extraction:

    Article Title: A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana
    Article Snippet: .. And then protein extraction buffer (1 mL) containing 50 mM 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES), 150 mM KCl, 1 mM ethylenediaminetetraacetate (EDTA) (pH 8.0), 0.1% triton X-100, 1 mM dl-dithiothreitol (DTT), and 1 × Protease Inhibitor Cocktail (Sigma) was added to 500 mg of each ground sample. .. Supernatants were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose blotting membrane.

    High Content Screening:

    Article Title: A Surface Plasmon Resonance-based assay to measure serum concentrations of therapeutic antibodies and anti-drug antibodies
    Article Snippet: MgCl2 , ethylenediaminetetraacetate (EDTA) and Tween 20 were from Sigma-Aldrich (Milan, Italy). .. HCA-216 was purchased from Bio-Rad Laboratories (Segrate, Italy) and used as calibrator for ATI.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Defects in ciliary localization of Nek8 is associated with cystogenesis
    Article Snippet: Transfected IMCD-3 cells were resuspended in 1x sodium dodecyl sulfate (SDS) sample buffer [10% glycerol, 50 mM Tris (pH 6.8), 2 mM ethylenediaminetetraacetate (EDTA), 2% SDS, 144 mM 2-mercaptoethanol, 0.01% bromophenol blue (all from Sigma)], and the suspension was sonicated to shear genomic DNA. .. The samples were separated by electrophoresis in an 8% SDS-polyacrylamide gel electrophoresis (PAGE) gel and transferred to Trans-Blot membrane (BioRad) using a wet transfer apparatus.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Flow Cytometric Evaluation of Human Neutrophil Apoptosis During Nitric Oxide Generation In Vitro: The Role of Exogenous Antioxidants
    Article Snippet: .. 3-morpholinosydnonimine hydrochloride (SIN-1), sodium nitroprusside (SNP), trypan blue, propidium iodide (PI), superoxide dismutase (SOD), catalase (CAT), fetal calf serum (FCS), 100 U/mL of penicillin and 100 μ g/mL of streptomycin, ethylenediaminetetraacetate (EDTA), ribonuclease A (RNase A) were obtained from Sigma (Sigma Chemical Co, St Louis, Mo). .. Phosphate-buffered saline (PBS), RPMI 1640 were purchased from Biomed (Lublin, Poland).

    SDS Page:

    Article Title: A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana
    Article Snippet: And then protein extraction buffer (1 mL) containing 50 mM 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES), 150 mM KCl, 1 mM ethylenediaminetetraacetate (EDTA) (pH 8.0), 0.1% triton X-100, 1 mM dl-dithiothreitol (DTT), and 1 × Protease Inhibitor Cocktail (Sigma) was added to 500 mg of each ground sample. .. Supernatants were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose blotting membrane.

    Electrophoresis:

    Article Title: Defects in ciliary localization of Nek8 is associated with cystogenesis
    Article Snippet: Transfected IMCD-3 cells were resuspended in 1x sodium dodecyl sulfate (SDS) sample buffer [10% glycerol, 50 mM Tris (pH 6.8), 2 mM ethylenediaminetetraacetate (EDTA), 2% SDS, 144 mM 2-mercaptoethanol, 0.01% bromophenol blue (all from Sigma)], and the suspension was sonicated to shear genomic DNA. .. The samples were separated by electrophoresis in an 8% SDS-polyacrylamide gel electrophoresis (PAGE) gel and transferred to Trans-Blot membrane (BioRad) using a wet transfer apparatus.

    Selection:

    Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors
    Article Snippet: .. Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C. .. MDDCs were generated by culturing purified monocytes in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% fetal calf serum (FCS), IL-4 (1,000 U/ml) and GM-CSF (1,000 U/ml) (Biosource, Carlsbad, CA) for 24 hours.

    In Situ:

    Article Title: Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages
    Article Snippet: .. The specimens were decalcified in 22.5% ethylenediaminetetraacetate (EDTA) and embedded in paraffin, and serial frontal sections of 7 μ m in thickness were prepared, stained with ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit (Millipore, Billerica, MA), and observed by light microscopy. ..

    Protein Binding:

    Article Title: Up-Regulation of Phosphorylated/Activated p70 S6 Kinase and Its Relationship to Neurofibrillary Pathology in Alzheimer's Disease
    Article Snippet: Microtiter plates (Nunc-Immuno, Roskilde, Denmark) were coated in duplicates with 2 μg of protein/well in 20 mmol/L Tris-HCl, pH 7.4, buffered with 2.5 mmol/L ethylenediaminetetraacetate (EDTA), 2.5 mmol/L EGTA, 0.1% (v/v) protease-inhibitor cocktail (Sigma-Aldrich, Stockholm, Sweden), 0.1% sodium azide, overnight at 4°C. .. Unspecific protein-binding sites were blocked with 3% BSA in 10 mmol/L Tris-HCl buffer with 0.85% NaCl and 0.2% Tween-20 for 1 hour at room temperature.

    Concentration Assay:

    Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors
    Article Snippet: Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C. .. MDMs were made by plastic adherence of monocytes in serum free media for 1 hour before supplemented with RPMI 1640 media containing human AB serum (Sigma Aldrich), which brought the final concentration of human serum to 10%.

    Staining:

    Article Title: Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages
    Article Snippet: .. The specimens were decalcified in 22.5% ethylenediaminetetraacetate (EDTA) and embedded in paraffin, and serial frontal sections of 7 μ m in thickness were prepared, stained with ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit (Millipore, Billerica, MA), and observed by light microscopy. ..

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  • 93
    Millipore ethylenediaminetetraacetate edta
    Ethylenediaminetetraacetate Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacetate edta/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacetate edta - by Bioz Stars, 2020-04
    93/100 stars
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