Structured Review

Roche ethylenediaminetetraacectic acid
Ethylenediaminetetraacectic Acid, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ethylenediaminetetraacectic acid/product/Roche
Average 94 stars, based on 4 article reviews
Price from $9.99 to $1999.99
ethylenediaminetetraacectic acid - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Nucleic Acid Electrophoresis:

Article Title: ORAI and Store-Operated Calcium Influx in Human Airway Smooth Muscle Cells
Article Snippet: Cells were lysed on ice with a lysis buffer consisting of a complete inhibitor cocktail + 1 mM ethylenediaminetetraacectic acid (cat. no. 11-836-153-001; Roche Diagnostics Ltd, West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 μg/ml pepstatin A. .. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was used to separate the proteins according to their molecular weight.

Transfection:

Article Title: ORAI and Store-Operated Calcium Influx in Human Airway Smooth Muscle Cells
Article Snippet: Western blot analysis was used to detect ORAI1 protein (∼50 kD) in HASM and to detect changes in protein level after siRNA transfection. .. Cells were lysed on ice with a lysis buffer consisting of a complete inhibitor cocktail + 1 mM ethylenediaminetetraacectic acid (cat. no. 11-836-153-001; Roche Diagnostics Ltd, West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 μg/ml pepstatin A.

Expressing:

Article Title: MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease
Article Snippet: Western blotting analysis Western blotting analysis was used to detect changes in lung tissue expression of MUC1-CT, TLR-4, phospho-ERK1/2, MKP1, GRα and phospho-GR-Ser226, phospho-p38. .. Lung tissue from human and mouse were homogenized and lysed on ice with a lysis buffer comprising a complete inhibitor cocktail plus 1 mM ethylenediaminetetraacectic acid (Roche Diagnostics Ltd., West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 mg/mL pepstatin A.

Marker:

Article Title: MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease
Article Snippet: Lung tissue from human and mouse were homogenized and lysed on ice with a lysis buffer comprising a complete inhibitor cocktail plus 1 mM ethylenediaminetetraacectic acid (Roche Diagnostics Ltd., West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 mg/mL pepstatin A. .. Briefly, 15 μg of proteins (denatured) along with a molecular weight protein marker (Bio-Rad Kaleidoscope marker; Bio-Rad Laboratories) were loaded onto an acrylamide gel consisting of a 5% acrylamide stacking gel stacked on top of a 10% acrylamide resolving gel and run through the gel by application of 100 V for 1 h. Proteins were transferred from the gel to a polyvinylidene difluoride membrane using a wet-blotting method.

Article Title: ORAI and Store-Operated Calcium Influx in Human Airway Smooth Muscle Cells
Article Snippet: Cells were lysed on ice with a lysis buffer consisting of a complete inhibitor cocktail + 1 mM ethylenediaminetetraacectic acid (cat. no. 11-836-153-001; Roche Diagnostics Ltd, West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 μg/ml pepstatin A. .. Briefly, 20 μg protein (denatured) along with a molecular weight protein marker, Bio-Rad Kaleidoscope marker (Bio-Rad Laboratories), was loaded onto an acrylamide gel consisting of a 5% acrylamide stacking gel stacked on top of a 10% acrylamide resolving gel and run through the gel by application of 100 V for approximately 1 hour.

Polyacrylamide Gel Electrophoresis:

Article Title: MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease
Article Snippet: Lung tissue from human and mouse were homogenized and lysed on ice with a lysis buffer comprising a complete inhibitor cocktail plus 1 mM ethylenediaminetetraacectic acid (Roche Diagnostics Ltd., West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 mg/mL pepstatin A. .. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was used to separate the proteins according to their molecular weight.

Lysis:

Article Title: MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease
Article Snippet: .. Lung tissue from human and mouse were homogenized and lysed on ice with a lysis buffer comprising a complete inhibitor cocktail plus 1 mM ethylenediaminetetraacectic acid (Roche Diagnostics Ltd., West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 mg/mL pepstatin A. ..

Article Title: ORAI and Store-Operated Calcium Influx in Human Airway Smooth Muscle Cells
Article Snippet: .. Cells were lysed on ice with a lysis buffer consisting of a complete inhibitor cocktail + 1 mM ethylenediaminetetraacectic acid (cat. no. 11-836-153-001; Roche Diagnostics Ltd, West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 μg/ml pepstatin A. .. The Bio-Rad assay (Bio-Rad Laboratories Ltd., Herts, UK) was used (following manufacturer's instructions) to quantify the level of protein in each sample to ensure equal protein loading.

Article Title: Roflumilast improves corticosteroid resistance COPD bronchial epithelial cells stimulated with toll like receptor 3 agonist
Article Snippet: .. Total protein was extracted using a lysis buffer consisting of a complete inhibitor cocktail plus 1 mM ethylenediaminetetraacectic acid (Roche Diagnostics Ltd, West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol and 1 μg/ml pepstatin A. ..

Acrylamide Gel Assay:

Article Title: MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease
Article Snippet: Lung tissue from human and mouse were homogenized and lysed on ice with a lysis buffer comprising a complete inhibitor cocktail plus 1 mM ethylenediaminetetraacectic acid (Roche Diagnostics Ltd., West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 mg/mL pepstatin A. .. Briefly, 15 μg of proteins (denatured) along with a molecular weight protein marker (Bio-Rad Kaleidoscope marker; Bio-Rad Laboratories) were loaded onto an acrylamide gel consisting of a 5% acrylamide stacking gel stacked on top of a 10% acrylamide resolving gel and run through the gel by application of 100 V for 1 h. Proteins were transferred from the gel to a polyvinylidene difluoride membrane using a wet-blotting method.

Article Title: ORAI and Store-Operated Calcium Influx in Human Airway Smooth Muscle Cells
Article Snippet: Cells were lysed on ice with a lysis buffer consisting of a complete inhibitor cocktail + 1 mM ethylenediaminetetraacectic acid (cat. no. 11-836-153-001; Roche Diagnostics Ltd, West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 μg/ml pepstatin A. .. Briefly, 20 μg protein (denatured) along with a molecular weight protein marker, Bio-Rad Kaleidoscope marker (Bio-Rad Laboratories), was loaded onto an acrylamide gel consisting of a 5% acrylamide stacking gel stacked on top of a 10% acrylamide resolving gel and run through the gel by application of 100 V for approximately 1 hour.

Western Blot:

Article Title: MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease
Article Snippet: Paragraph title: Western blotting analysis ... Lung tissue from human and mouse were homogenized and lysed on ice with a lysis buffer comprising a complete inhibitor cocktail plus 1 mM ethylenediaminetetraacectic acid (Roche Diagnostics Ltd., West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 mg/mL pepstatin A.

Article Title: ORAI and Store-Operated Calcium Influx in Human Airway Smooth Muscle Cells
Article Snippet: Paragraph title: Western Blot Analysis ... Cells were lysed on ice with a lysis buffer consisting of a complete inhibitor cocktail + 1 mM ethylenediaminetetraacectic acid (cat. no. 11-836-153-001; Roche Diagnostics Ltd, West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 μg/ml pepstatin A.

Molecular Weight:

Article Title: MUC1 deficiency mediates corticosteroid resistance in chronic obstructive pulmonary disease
Article Snippet: Lung tissue from human and mouse were homogenized and lysed on ice with a lysis buffer comprising a complete inhibitor cocktail plus 1 mM ethylenediaminetetraacectic acid (Roche Diagnostics Ltd., West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 mg/mL pepstatin A. .. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was used to separate the proteins according to their molecular weight.

Article Title: ORAI and Store-Operated Calcium Influx in Human Airway Smooth Muscle Cells
Article Snippet: Cells were lysed on ice with a lysis buffer consisting of a complete inhibitor cocktail + 1 mM ethylenediaminetetraacectic acid (cat. no. 11-836-153-001; Roche Diagnostics Ltd, West Sussex, UK) with 20 mM Tris base, 0.9% NaCl, 0.1% Triton X-100, 1 mM dithiothreitol, and 1 μg/ml pepstatin A. .. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was used to separate the proteins according to their molecular weight.

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    Roche ethylenediaminetetraacectic acid
    Ethylenediaminetetraacectic Acid, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetetraacectic acid/product/Roche
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetetraacectic acid - by Bioz Stars, 2020-04
    94/100 stars
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