eschericia coli strain top10  (Thermo Fisher)


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    Structured Review

    Thermo Fisher eschericia coli strain top10
    Eschericia Coli Strain Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eschericia coli strain top10/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    eschericia coli strain top10 - by Bioz Stars, 2020-04
    85/100 stars

    Related Products / Commonly Used Together

    pcr primers
    pcdna3
    individual 186 bp fragments
    erroneous ej

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    Related Articles

    Clone Assay:

    Article Title: The coenzyme A disulfide reductase of Borrelia burgdorferi is important for rapid growth throughout the enzootic cycle and essential for infection of the mammalian host
    Article Snippet: .. All PCR primers used in this study are listed in .Routine cloning and plasmid propagation were performed using Eschericia coli strain Top10 (Invitrogen). ..

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set. .. The PCR product was then digested with EcoRI or EcoRV to determine whether the original restriction site is restored by end joining without nucleotide alteration.

    Selection:

    Article Title: The coenzyme A disulfide reductase of Borrelia burgdorferi is important for rapid growth throughout the enzootic cycle and essential for infection of the mammalian host
    Article Snippet: All PCR primers used in this study are listed in .Routine cloning and plasmid propagation were performed using Eschericia coli strain Top10 (Invitrogen). .. Solid phase selection was performed on LB agar plates (LB with 1.5% agar) supplemented with the appropriate antibiotic.

    Agarose Gel Electrophoresis:

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: PCR products were separated in agarose gel and visualized by staining with ethidium bromide. .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set.

    In Vitro:

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: Paragraph title: 2.4. In vitro DNA EJ assay ... This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set.

    Electrophoresis:

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: Erroneous non microhomology-mediated end-joining (NMEJ) can occur with few nucleotide alterations at the end-joined sites, undetectable by electrophoresis in agarose gels. .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set.

    Incubation:

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: Briefly, cell lysates were incubated with the EcoRI or EcoRV-linearized plasmid at 37°C for 2 hours. .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set.

    Amplification:

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set. .. The PCR product was then digested with EcoRI or EcoRV to determine whether the original restriction site is restored by end joining without nucleotide alteration.

    Activity Assay:

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set. .. The PCR fragment resistant to the enzyme digestion was considered to be the product of erroneous DNA EJ activity [ , , ].

    Polymerase Chain Reaction:

    Article Title: The coenzyme A disulfide reductase of Borrelia burgdorferi is important for rapid growth throughout the enzootic cycle and essential for infection of the mammalian host
    Article Snippet: .. All PCR primers used in this study are listed in .Routine cloning and plasmid propagation were performed using Eschericia coli strain Top10 (Invitrogen). ..

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set. .. The PCR product was then digested with EcoRI or EcoRV to determine whether the original restriction site is restored by end joining without nucleotide alteration.

    Staining:

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: PCR products were separated in agarose gel and visualized by staining with ethidium bromide. .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set.

    Transformation Assay:

    Article Title: The coenzyme A disulfide reductase of Borrelia burgdorferi is important for rapid growth throughout the enzootic cycle and essential for infection of the mammalian host
    Article Snippet: All PCR primers used in this study are listed in .Routine cloning and plasmid propagation were performed using Eschericia coli strain Top10 (Invitrogen). .. Preparation and transformation of chemically-competent E. coli were performed as described previously ( ).

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set. .. The PCR product was then digested with EcoRI or EcoRV to determine whether the original restriction site is restored by end joining without nucleotide alteration.

    Plasmid Preparation:

    Article Title: The coenzyme A disulfide reductase of Borrelia burgdorferi is important for rapid growth throughout the enzootic cycle and essential for infection of the mammalian host
    Article Snippet: .. All PCR primers used in this study are listed in .Routine cloning and plasmid propagation were performed using Eschericia coli strain Top10 (Invitrogen). ..

    Article Title: p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G
    Article Snippet: .. This erroneous EJ can be detected by the following procedures: Individual 186 bp fragments were cloned in pcDNA3.1/V5-His TOPO plasmid (Invitrogen), transformed into Eschericia coli strain TOP10 (Invitrogen), and amplified by single colony PCR using the M13 primer set. .. The PCR product was then digested with EcoRI or EcoRV to determine whether the original restriction site is restored by end joining without nucleotide alteration.

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    Thermo Fisher one shot top10 electrocomp e coli
    One Shot Top10 Electrocomp E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher growth conditions escherichia coli top10
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    Thermo Fisher one shot top10 chemically competent e coli
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