escherichiacoli strain top10  (Thermo Fisher)


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  • 88

    Structured Review

    Thermo Fisher escherichiacoli strain top10
    Escherichiacoli Strain Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichiacoli strain top10/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichiacoli strain top10 - by Bioz Stars, 2020-04
    88/100 stars

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    Related Articles

    Clone Assay:

    Article Title: DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
    Article Snippet: Escherichiacoli strain TOP10 (Invitrogen, USA) was cultivated in Luria-Bertani (LB) medium at 37ºC with the addition of ampicillin to 100 µg.mL-1 . .. Then, it was cloned into pTARGET (Promega, USA) and pAE vectors ( ) for use as DNA and subunit vaccines, respectively, as described ( ).

    Amplification:

    Article Title: DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
    Article Snippet: Escherichiacoli strain TOP10 (Invitrogen, USA) was cultivated in Luria-Bertani (LB) medium at 37ºC with the addition of ampicillin to 100 µg.mL-1 . .. Vaccine construction - The DNA sequence corresponding to theligBrep fragment (1-1,884 bp) was amplified by polymerase chain reaction (PCR) from the L. interrogans serovar Canicola strain HU genome using oligonucleotides designed according to the genome sequence ofL.interrogans serovar Copenhageni strain Fiocruz L1-130 (GenBank accession AE016823).

    Sequencing:

    Article Title: DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
    Article Snippet: Escherichiacoli strain TOP10 (Invitrogen, USA) was cultivated in Luria-Bertani (LB) medium at 37ºC with the addition of ampicillin to 100 µg.mL-1 . .. Vaccine construction - The DNA sequence corresponding to theligBrep fragment (1-1,884 bp) was amplified by polymerase chain reaction (PCR) from the L. interrogans serovar Canicola strain HU genome using oligonucleotides designed according to the genome sequence ofL.interrogans serovar Copenhageni strain Fiocruz L1-130 (GenBank accession AE016823).

    Expressing:

    Article Title: DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
    Article Snippet: Escherichiacoli strain TOP10 (Invitrogen, USA) was cultivated in Luria-Bertani (LB) medium at 37ºC with the addition of ampicillin to 100 µg.mL-1 . .. Briefly, for DNA vaccine construction, the fragment amplified by PCR was cloned into the pTARGETTM mammalian expression vector.

    Polymerase Chain Reaction:

    Article Title: DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
    Article Snippet: Escherichiacoli strain TOP10 (Invitrogen, USA) was cultivated in Luria-Bertani (LB) medium at 37ºC with the addition of ampicillin to 100 µg.mL-1 . .. Vaccine construction - The DNA sequence corresponding to theligBrep fragment (1-1,884 bp) was amplified by polymerase chain reaction (PCR) from the L. interrogans serovar Canicola strain HU genome using oligonucleotides designed according to the genome sequence ofL.interrogans serovar Copenhageni strain Fiocruz L1-130 (GenBank accession AE016823).

    Plasmid Preparation:

    Article Title: DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
    Article Snippet: Escherichiacoli strain TOP10 (Invitrogen, USA) was cultivated in Luria-Bertani (LB) medium at 37ºC with the addition of ampicillin to 100 µg.mL-1 . .. Briefly, for DNA vaccine construction, the fragment amplified by PCR was cloned into the pTARGETTM mammalian expression vector.