escherichia coli strains jm109  (Thermo Fisher)


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    Structured Review

    Thermo Fisher escherichia coli strains jm109
    Escherichia Coli Strains Jm109, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strains jm109/product/Thermo Fisher
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strains jm109 - by Bioz Stars, 2020-01
    77/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Evaluation of the Recombinant Protein TpF1 of Treponema pallidum for Serodiagnosis of Syphilis
    Article Snippet: .. E. coli strain JM109 (Invitrogen) was used for DNA cloning, and E. coli strain BL21(DE3)(Novagen, Merck Millipore, Germany) was used for protein expression. .. The expression vector pET28a was purchased from Novagen (Merck Millipore, Germany).

    Article Title: Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides
    Article Snippet: .. This fragment was cloned in E. coli strain JM109 by using the pCR2.1 (Invitrogen) vector and sequenced. ..

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations. .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen).

    Inverse PCR:

    Article Title: Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides
    Article Snippet: This fragment was cloned in E. coli strain JM109 by using the pCR2.1 (Invitrogen) vector and sequenced. .. The 948-bp product was used to design primers FTFC1i (Table ) and FTFC2 (Table ) for inverse PCR purposes.

    Subcloning:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations. .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen).

    Microscopy:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: Cultures were examined by phase-contrast microscopy for purity and typical strain morphology before use. .. Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations.

    Sequencing:

    Article Title: Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides
    Article Snippet: Paragraph title: Identification and nucleotide sequence analysis of the FTF gene. ... This fragment was cloned in E. coli strain JM109 by using the pCR2.1 (Invitrogen) vector and sequenced.

    Expressing:

    Article Title: Evaluation of the Recombinant Protein TpF1 of Treponema pallidum for Serodiagnosis of Syphilis
    Article Snippet: .. E. coli strain JM109 (Invitrogen) was used for DNA cloning, and E. coli strain BL21(DE3)(Novagen, Merck Millipore, Germany) was used for protein expression. .. The expression vector pET28a was purchased from Novagen (Merck Millipore, Germany).

    Article Title: Multiple Formate Dehydrogenase Enzymes in the Facultative Methylotroph Methylobacterium extorquens AM1 Are Dispensable for Growth on Methanol
    Article Snippet: Escherichia coli strains JM109 (Invitrogen), Top10 (Invitrogen), and S17-1 ( ) were grown in Luria-Bertani (LB) medium in the presence of appropriate antibiotics as described by Sambrook et al. ( ). .. To test the dependence of expression of formate dehydrogenases on molybdenum and tungsten, a similar medium was used from which (NH4 )Mo7 O21 was omitted.

    Article Title: Construction of recombinant eukaryotic expression plasmid containing murine CD40 ligand gene and its expression in H22 cells
    Article Snippet: The eukaryotic expression vector pcDNA3.1+ was presented by Dr. Chun Deng, Michigan College, USA. .. E.coli strain JM109 was purchased from Invitrogen Life Technologies (Burlington, ON, Canada).

    Polymerase Chain Reaction:

    Article Title: Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides
    Article Snippet: By using the degenerated primers 5FTF and 6FTFi, based on the conserved amino acid sequences of FTFs of gram-positive origin (Fig. ), the chromosomal DNA of L. reuteri strain 121, and DNA polymerase, a PCR product of 234 bp was obtained (Fig. ). .. This fragment was cloned in E. coli strain JM109 by using the pCR2.1 (Invitrogen) vector and sequenced.

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations. .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen).

    Over Expression:

    Article Title: Comparative structural, kinetic and inhibitor studies of Trypanosoma brucei trypanothione reductase with T. cruzi
    Article Snippet: .. 2.1 Organisms and reagents Routine plasmid manipulations were performed in Escherichia coli strain JM109 and over-expression in strain BL21 Star (DE3)pLysS (Invitrogen). .. All chemicals were of the highest grade available from Sigma, BDH and Molecular Probes.

    Plasmid Preparation:

    Article Title: Comparative structural, kinetic and inhibitor studies of Trypanosoma brucei trypanothione reductase with T. cruzi
    Article Snippet: .. 2.1 Organisms and reagents Routine plasmid manipulations were performed in Escherichia coli strain JM109 and over-expression in strain BL21 Star (DE3)pLysS (Invitrogen). .. All chemicals were of the highest grade available from Sigma, BDH and Molecular Probes.

    Article Title: Evaluation of the Recombinant Protein TpF1 of Treponema pallidum for Serodiagnosis of Syphilis
    Article Snippet: E. coli strain JM109 (Invitrogen) was used for DNA cloning, and E. coli strain BL21(DE3)(Novagen, Merck Millipore, Germany) was used for protein expression. .. The expression vector pET28a was purchased from Novagen (Merck Millipore, Germany).

    Article Title: Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides
    Article Snippet: .. This fragment was cloned in E. coli strain JM109 by using the pCR2.1 (Invitrogen) vector and sequenced. ..

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations. .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen).

    Article Title: Construction of recombinant eukaryotic expression plasmid containing murine CD40 ligand gene and its expression in H22 cells
    Article Snippet: The T vector pUCm-T was provided by the Bioasia, Shanghai. .. E.coli strain JM109 was purchased from Invitrogen Life Technologies (Burlington, ON, Canada).

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    Thermo Fisher e coli jm109 strain
    E Coli Jm109 Strain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli jm109 strain/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli jm109 strain - by Bioz Stars, 2020-01
    79/100 stars
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    82
    Thermo Fisher escherichia coli strain jm109 cells
    Escherichia Coli Strain Jm109 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain jm109 cells/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain jm109 cells - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

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