escherichia coli strains jm109  (Thermo Fisher)


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    Structured Review

    Thermo Fisher escherichia coli strains jm109
    Escherichia Coli Strains Jm109, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strains jm109/product/Thermo Fisher
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strains jm109 - by Bioz Stars, 2019-12
    77/100 stars

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    Related Articles

    Molecular Cloning:

    Article Title: Biochemical characterization of a novel ArsA ATPase complex from Alkaliphilus metalliredigens QYMF
    Article Snippet: While ArsB exchanges As(III) with protons, Acr3 does not, but how it is coupled to the proton-motive force is still unclear. .. E. coli strain JM109 and TOP10 (Invitrogen) were used for molecular cloning and protein expression while AW3110 ( ΔarsRBC ) was used for phenotypic assays. .. Plasmids pETDuet-1 (Novagen), pTrcHis2A and pTrcHis2B (Invitrogen) were used as cloning and expression vectors.

    Clone Assay:

    Article Title: Evaluation of the Recombinant Protein TpF1 of Treponema pallidum for Serodiagnosis of Syphilis
    Article Snippet: All animal experiments were approved by the Animal Welfare Committee of the University of South China and conducted in accordance with the regulations of the institution. .. E. coli strain JM109 (Invitrogen) was used for DNA cloning, and E. coli strain BL21(DE3)(Novagen, Merck Millipore, Germany) was used for protein expression. .. The expression vector pET28a was purchased from Novagen (Merck Millipore, Germany).

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations. .. Plasmid pVA2198 ( ) was used as the source of the ermF/AM gene cassette.

    Subcloning:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: Cultures were examined by phase-contrast microscopy for purity and typical strain morphology before use. .. Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations. .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen).

    Microscopy:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: Cultures were examined by phase-contrast microscopy for purity and typical strain morphology before use. .. Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations.

    Expressing:

    Article Title: Biochemical characterization of a novel ArsA ATPase complex from Alkaliphilus metalliredigens QYMF
    Article Snippet: While ArsB exchanges As(III) with protons, Acr3 does not, but how it is coupled to the proton-motive force is still unclear. .. E. coli strain JM109 and TOP10 (Invitrogen) were used for molecular cloning and protein expression while AW3110 ( ΔarsRBC ) was used for phenotypic assays. .. Plasmids pETDuet-1 (Novagen), pTrcHis2A and pTrcHis2B (Invitrogen) were used as cloning and expression vectors.

    Article Title: Evaluation of the Recombinant Protein TpF1 of Treponema pallidum for Serodiagnosis of Syphilis
    Article Snippet: All animal experiments were approved by the Animal Welfare Committee of the University of South China and conducted in accordance with the regulations of the institution. .. E. coli strain JM109 (Invitrogen) was used for DNA cloning, and E. coli strain BL21(DE3)(Novagen, Merck Millipore, Germany) was used for protein expression. .. The expression vector pET28a was purchased from Novagen (Merck Millipore, Germany).

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations. .. Plasmid pVA2198 ( ) was used as the source of the ermF/AM gene cassette.

    Article Title: Multiple Formate Dehydrogenase Enzymes in the Facultative Methylotroph Methylobacterium extorquens AM1 Are Dispensable for Growth on Methanol
    Article Snippet: Escherichia coli strains JM109 (Invitrogen), Top10 (Invitrogen), and S17-1 ( ) were grown in Luria-Bertani (LB) medium in the presence of appropriate antibiotics as described by Sambrook et al. ( ). .. Escherichia coli strains JM109 (Invitrogen), Top10 (Invitrogen), and S17-1 ( ) were grown in Luria-Bertani (LB) medium in the presence of appropriate antibiotics as described by Sambrook et al. ( ).

    Article Title: Construction of recombinant eukaryotic expression plasmid containing murine CD40 ligand gene and its expression in H22 cells
    Article Snippet: The eukaryotic expression vector pcDNA3.1+ was presented by Dr. Chun Deng, Michigan College, USA. .. E.coli strain JM109 was purchased from Invitrogen Life Technologies (Burlington, ON, Canada).

    Over Expression:

    Article Title: Comparative structural, kinetic and inhibitor studies of Trypanosoma brucei trypanothione reductase with T. cruzi
    Article Snippet: The information on the enzyme from T. b. brucei is also of particular relevance since it is identical at the amino acid level to the putative TryR from T. b. gambiense , the causative agent of over 90% of reported HAT cases . .. 2.1 Routine plasmid manipulations were performed in Escherichia coli strain JM109 and over-expression in strain BL21 Star (DE3)pLysS (Invitrogen). .. All chemicals were of the highest grade available from Sigma, BDH and Molecular Probes.

    Plasmid Preparation:

    Article Title: Comparative structural, kinetic and inhibitor studies of Trypanosoma brucei trypanothione reductase with T. cruzi
    Article Snippet: The information on the enzyme from T. b. brucei is also of particular relevance since it is identical at the amino acid level to the putative TryR from T. b. gambiense , the causative agent of over 90% of reported HAT cases . .. 2.1 Routine plasmid manipulations were performed in Escherichia coli strain JM109 and over-expression in strain BL21 Star (DE3)pLysS (Invitrogen). .. All chemicals were of the highest grade available from Sigma, BDH and Molecular Probes.

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: Cultures were examined by phase-contrast microscopy for purity and typical strain morphology before use. .. Escherichia coli strains JM109 ( ) and TOP10 (Invitrogen, Carlsbad, Calif.) were used as hosts for routine subcloning and plasmid preparations. .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen).

    Article Title: Construction of recombinant eukaryotic expression plasmid containing murine CD40 ligand gene and its expression in H22 cells
    Article Snippet: The T vector pUCm-T was provided by the Bioasia, Shanghai. .. E.coli strain JM109 was purchased from Invitrogen Life Technologies (Burlington, ON, Canada).