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Stratagene escherichia coli strains jm109
Escherichia Coli Strains Jm109, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli strains jm109 - by Bioz Stars, 2020-01
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Transduction:

Article Title: Sequence Changes in the Ton Box Region of BtuB Affect Its Transport Activities and Interaction with TonB Protein
Article Snippet: E. coli strain JM109 (Stratagene) was used as the host for plasmid construction and maintenance. .. The tonB ::Km allele was introduced by P1 transduction from strain KP1032, which was obtained from K. Postle.

Clone Assay:

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: Paragraph title: Gene cloning, purification, crystallization, and data collection ... The E. coli strain JM109 (Stratagene) was transformed with phGK.

Article Title: A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice
Article Snippet: .. Escherichia coli strains JM109 and XL-10 (Stratagene) were used for cloning and were grown in Luria-Bertani (LB) broth. .. M. tuberculosis strains were maintained in 7H10 agar (Difco) supplemented with 0.2% glycerol, 0.05% Tween 80, and 10% ADN supplement (0.5% albumin, 0.2% dextrose, 0.085% NaCl).

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI). .. Due to the usage of the NcoI site the second amino acid of χ was changed from Lys to Ala. After cloning the gene was completely sequenced and no further mutations were detected.

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: E. coli strain INV′′F′ and plasmid pCRII, purchased from Invitrogen (Carlsbad, Calif.) as the TA cloning kit, were used for PCR cloning. .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

Article Title: MntR(Rv2788) a transcriptional regulator that controls manganese homeostasis in Mycobacterium tuberculosis
Article Snippet: .. Escherichia coli strains JM109, XL-10 (stratagene) and HB101 used for cloning were grown in Luria-Bertani (LB) media. .. M. tuberculosis H37Rv and derived strains were maintained in 7H10 agar or 7H9 broth (Difco) supplemented with 0.2% glycerol, 0.05% Tween 80 and ADN (0.5% BSA, 0.2% dextrose and 0.085% NaCl).

Centrifugation:

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: The E. coli strain JM109 (Stratagene) was transformed with phGK. .. Isopropyl-β- d -thiogalactopyranoside was added to induce the enzyme to the medium, and the cell cultivation was continued for 3 h. Cells were harvested by centrifugation, suspended in 10 mM Tris/HCl buffer (pH 8.0), and disrupted by ultrasonication.

Amplification:

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: The amplified 1.4-kb fragment was digested with Pst I and Hin dIII and ligated with the expression vector pTrcHis linearized with Pst I and Hin dIII to generate phGK. .. The E. coli strain JM109 (Stratagene) was transformed with phGK.

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI). .. Due to the usage of the NcoI site the second amino acid of χ was changed from Lys to Ala. After cloning the gene was completely sequenced and no further mutations were detected.

Filtration:

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: After 48 h of growth, the fungal biomass was harvested by filtration. .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

TA Cloning:

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: E. coli strain INV′′F′ and plasmid pCRII, purchased from Invitrogen (Carlsbad, Calif.) as the TA cloning kit, were used for PCR cloning. .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

Construct:

Article Title: Cloning and Expression of Cytochrome P450 Enzymes Catalyzing the Conversion of Tyrosine to p-Hydroxyphenylacetaldoxime in the Biosynthesis of Cyanogenic Glucosides in Triglochin maritima 1
Article Snippet: .. All expression constructs were transformed into the E. coli strains JM109 (Stratagene) and XL-1 Blue (Stratagene). .. The native and Δ(1–52)2E1(10aa) constructs of clone #1 and the construct of clone #2 were co-transformed with pSBET , which encodes a tRNA gene for rare Arg codons, into JM109.

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: Orpinomyces sp. strain PC-2 isolated from the rumen of a cow ( ) was cultured with Avicel or oat spelt xylan (1.0%, wt/vol) as the carbon source, and a cDNA library was constructed as previously described ( ). .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

Expressing:

Article Title: DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange
Article Snippet: .. Proteins Hexahistidine-tagged human RAD51 was overexpressed in the Escherichia coli strain JM109 (DE3), which also carried an expression vector for the minor tRNAs (Codon(+)RIL, Stratagene, La Jolla, CA, USA). .. The RAD51 expressed in the E. coli strain was purified by a four-step method, as described previously ( ).

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: The amplified 1.4-kb fragment was digested with Pst I and Hin dIII and ligated with the expression vector pTrcHis linearized with Pst I and Hin dIII to generate phGK. .. The E. coli strain JM109 (Stratagene) was transformed with phGK.

Article Title: Cloning and Expression of Cytochrome P450 Enzymes Catalyzing the Conversion of Tyrosine to p-Hydroxyphenylacetaldoxime in the Biosynthesis of Cyanogenic Glucosides in Triglochin maritima 1
Article Snippet: .. All expression constructs were transformed into the E. coli strains JM109 (Stratagene) and XL-1 Blue (Stratagene). .. The native and Δ(1–52)2E1(10aa) constructs of clone #1 and the construct of clone #2 were co-transformed with pSBET , which encodes a tRNA gene for rare Arg codons, into JM109.

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: As they carry the ssb gene under control of the λ pL promotor, protein expression was induced by a temperature shift from 30 to 42°C and cells were harvested 3 h after induction. .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI).

Modification:

Article Title: Cloning and Expression of Cytochrome P450 Enzymes Catalyzing the Conversion of Tyrosine to p-Hydroxyphenylacetaldoxime in the Biosynthesis of Cyanogenic Glucosides in Triglochin maritima 1
Article Snippet: All expression constructs were transformed into the E. coli strains JM109 (Stratagene) and XL-1 Blue (Stratagene). .. Single colonies were grown overnight in Luria-Bertani medium (50 μg/mL ampicillin, 37°C, 225 rpm) and used to inoculate 100× volume of modified TB medium (50 μg/mL ampicillin, 1 m m thiamine, 75 μg/mL δ-amino-levulinic acid, 1 m m isopropyl β- d -thiogalactopyranoside [IPTG], 28°C, 125 rpm, 48 h).

Transformation Assay:

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: .. The E. coli strain JM109 (Stratagene) was transformed with phGK. ..

Article Title: Cloning and Expression of Cytochrome P450 Enzymes Catalyzing the Conversion of Tyrosine to p-Hydroxyphenylacetaldoxime in the Biosynthesis of Cyanogenic Glucosides in Triglochin maritima 1
Article Snippet: .. All expression constructs were transformed into the E. coli strains JM109 (Stratagene) and XL-1 Blue (Stratagene). .. The native and Δ(1–52)2E1(10aa) constructs of clone #1 and the construct of clone #2 were co-transformed with pSBET , which encodes a tRNA gene for rare Arg codons, into JM109.

Recombinase Polymerase Amplification:

Article Title: DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange
Article Snippet: Proteins Hexahistidine-tagged human RAD51 was overexpressed in the Escherichia coli strain JM109 (DE3), which also carried an expression vector for the minor tRNAs (Codon(+)RIL, Stratagene, La Jolla, CA, USA). .. Human RPA was produced in E. coli cells, and was prepared according to the published protocol ( ).

Crystallization Assay:

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: Paragraph title: Gene cloning, purification, crystallization, and data collection ... The E. coli strain JM109 (Stratagene) was transformed with phGK.

Derivative Assay:

Article Title: MntR(Rv2788) a transcriptional regulator that controls manganese homeostasis in Mycobacterium tuberculosis
Article Snippet: Escherichia coli strains JM109, XL-10 (stratagene) and HB101 used for cloning were grown in Luria-Bertani (LB) media. .. M. tuberculosis H37Rv and derived strains were maintained in 7H10 agar or 7H9 broth (Difco) supplemented with 0.2% glycerol, 0.05% Tween 80 and ADN (0.5% BSA, 0.2% dextrose and 0.085% NaCl).

Countercurrent Chromatography:

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI). .. Due to the usage of the NcoI site the second amino acid of χ was changed from Lys to Ala. After cloning the gene was completely sequenced and no further mutations were detected.

Cell Culture:

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: Orpinomyces sp. strain PC-2 isolated from the rumen of a cow ( ) was cultured with Avicel or oat spelt xylan (1.0%, wt/vol) as the carbon source, and a cDNA library was constructed as previously described ( ). .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

Polymerase Chain Reaction:

Article Title: Structural Organization of the pFra Virulence-Associated Plasmid of Rhamnose-Positive Yersinia pestis
Article Snippet: Loss of the pYV replicon was confirmed by plasmid screening and by PCR performed with primers YopP8 (5′-GAGACCAGTTCTTTAATCAG-3′) and YopP9 (5′-GCCAGTGCCAAACTAAAAAT-3′) (35 cycles consisting of 30 s at 94°C, 30 s at 50°C, and 30 s at 72°C). .. A spontaneous Nalr mutant of Escherichia coli strain JM109 (Stratagene, La Jolla, Calif.) was obtained from the strain collection of the Max von Pettenkofer Institute.

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: The following set of oligonucleotide primers was used to amplify the ADPGK gene fragment by PCR: The primer (5′-CCGCTGCAGATGACGAATTGGGAAAGTC TATAT-3′) introduced a unique Pst I restriction site; the other (5′-GGCAAGCTTTCAGTGAAGAGAAAACTCGGAAAC-3′), a unique Hin dIII restriction site. .. The E. coli strain JM109 (Stratagene) was transformed with phGK.

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI). .. Due to the usage of the NcoI site the second amino acid of χ was changed from Lys to Ala. After cloning the gene was completely sequenced and no further mutations were detected.

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: E. coli strain INV′′F′ and plasmid pCRII, purchased from Invitrogen (Carlsbad, Calif.) as the TA cloning kit, were used for PCR cloning. .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

DNA Extraction:

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: Paragraph title: Organisms and DNA isolation. ... E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

Mutagenesis:

Article Title: Structural Organization of the pFra Virulence-Associated Plasmid of Rhamnose-Positive Yersinia pestis
Article Snippet: .. A spontaneous Nalr mutant of Escherichia coli strain JM109 (Stratagene, La Jolla, Calif.) was obtained from the strain collection of the Max von Pettenkofer Institute. ..

Isolation:

Article Title: Structural Organization of the pFra Virulence-Associated Plasmid of Rhamnose-Positive Yersinia pestis
Article Snippet: Paragraph title: Bacterial strains, media, and plasmid isolation. ... A spontaneous Nalr mutant of Escherichia coli strain JM109 (Stratagene, La Jolla, Calif.) was obtained from the strain collection of the Max von Pettenkofer Institute.

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: The chromosomal P. horikoshii DNA was isolated as described previously ( ) and used as the template. .. The E. coli strain JM109 (Stratagene) was transformed with phGK.

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: Orpinomyces sp. strain PC-2 isolated from the rumen of a cow ( ) was cultured with Avicel or oat spelt xylan (1.0%, wt/vol) as the carbon source, and a cDNA library was constructed as previously described ( ). .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

Purification:

Article Title: DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange
Article Snippet: Proteins Hexahistidine-tagged human RAD51 was overexpressed in the Escherichia coli strain JM109 (DE3), which also carried an expression vector for the minor tRNAs (Codon(+)RIL, Stratagene, La Jolla, CA, USA). .. The RAD51 expressed in the E. coli strain was purified by a four-step method, as described previously ( ).

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: Paragraph title: Gene cloning, purification, crystallization, and data collection ... The E. coli strain JM109 (Stratagene) was transformed with phGK.

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: EcoSSB wild type was purified as described by Lohman et al . , whereas HsmtSSB and EcoSSB Q152* were purified as previously described for HsmtSSB ( ). .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI).

Sequencing:

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: The plasmid DNA pTrcHis, which carries an N-terminal His-Tag sequence, was obtained from Invitrogen. .. The E. coli strain JM109 (Stratagene) was transformed with phGK.

cDNA Library Assay:

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: Orpinomyces sp. strain PC-2 isolated from the rumen of a cow ( ) was cultured with Avicel or oat spelt xylan (1.0%, wt/vol) as the carbon source, and a cDNA library was constructed as previously described ( ). .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production.

Chloramphenicol Acetyltransferase Assay:

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI). .. Due to the usage of the NcoI site the second amino acid of χ was changed from Lys to Ala. After cloning the gene was completely sequenced and no further mutations were detected.

Plasmid Preparation:

Article Title: The MalF P2 Loop of the ATP-Binding Cassette Transporter MalFGK2 from Escherichia coli and Salmonella enterica Serovar Typhimurium Interacts with Maltose Binding Protein (MalE) throughout the Catalytic Cycle ▿
Article Snippet: E. coli strain JM109 (Stratagene) served as a host for the plasmids listed in Table . .. The plasmid-borne malK alleles originated from the Salmonella enterica subsp. enterica serovar, whereas the malF malG malE alleles were from E. coli .

Article Title: Structural Organization of the pFra Virulence-Associated Plasmid of Rhamnose-Positive Yersinia pestis
Article Snippet: Paragraph title: Bacterial strains, media, and plasmid isolation. ... A spontaneous Nalr mutant of Escherichia coli strain JM109 (Stratagene, La Jolla, Calif.) was obtained from the strain collection of the Max von Pettenkofer Institute.

Article Title: DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange
Article Snippet: .. Proteins Hexahistidine-tagged human RAD51 was overexpressed in the Escherichia coli strain JM109 (DE3), which also carried an expression vector for the minor tRNAs (Codon(+)RIL, Stratagene, La Jolla, CA, USA). .. The RAD51 expressed in the E. coli strain was purified by a four-step method, as described previously ( ).

Article Title: Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-? resolution: A large conformational change in ADP-dependent glucokinase
Article Snippet: The amplified 1.4-kb fragment was digested with Pst I and Hin dIII and ligated with the expression vector pTrcHis linearized with Pst I and Hin dIII to generate phGK. .. The E. coli strain JM109 (Stratagene) was transformed with phGK.

Article Title: Sequence Changes in the Ton Box Region of BtuB Affect Its Transport Activities and Interaction with TonB Protein
Article Snippet: .. E. coli strain JM109 (Stratagene) was used as the host for plasmid construction and maintenance. ..

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: Plasmids used for overproduction of SSB proteins were used to transform the E.coli strain BT317 ( ) containing the plasmid pRK248 coding for the thermosensitive λcI857 repressor. .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI).

Article Title: Noncatalytic Docking Domains of Cellulosomes of Anaerobic Fungi
Article Snippet: .. E. coli strains JM109 and BL21(DE3), from Stratagene (La Jolla, Calif.), and plasmid pRSETB, from Invitrogen, were used for heterologous protein production. ..

Positron Emission Tomography:

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI). .. Due to the usage of the NcoI site the second amino acid of χ was changed from Lys to Ala. After cloning the gene was completely sequenced and no further mutations were detected.

Produced:

Article Title: DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange
Article Snippet: Proteins Hexahistidine-tagged human RAD51 was overexpressed in the Escherichia coli strain JM109 (DE3), which also carried an expression vector for the minor tRNAs (Codon(+)RIL, Stratagene, La Jolla, CA, USA). .. Human RPA was produced in E. coli cells, and was prepared according to the published protocol ( ).

Concentration Assay:

Article Title: Structural Organization of the pFra Virulence-Associated Plasmid of Rhamnose-Positive Yersinia pestis
Article Snippet: A spontaneous Nalr mutant of Escherichia coli strain JM109 (Stratagene, La Jolla, Calif.) was obtained from the strain collection of the Max von Pettenkofer Institute. .. For plasmid maintenance and mutagenesis, nalidixic acid (20 μg/ml), chloramphenicol (30 μg/ml), tetracycline (12 μg/ml), and ampicillin (100 μg/ml) were added to the culture media as required. l -Arabinose (Sigma) at a concentration of 1 mM was used for induction of the Red system genes ( ) on helper plasmid pKD46.

CTG Assay:

Article Title: DNA polymerase III ? subunit ties single-stranded DNA binding protein to the bacterial replication machinery
Article Snippet: .. The χ gene was amplified from genomic DNA of the E.coli strain JM109 (Stratagene, La Jolla, CA) by PCR using Pfu DNA polymerase (forward primer, 5′-CGG AAG CCC ATG GCA AAC GCG ACG TTC TAC CTT CTG GAC AAT-3′; reverse primer, 5′-GGA AGC TCG AGT CAT TTC CAG GTT GCC GTA TTC AGG TTG AAA-3′) and cloned into the NcoI and XhoI sites of pET-15b (Novagen, Madison, WI). .. Due to the usage of the NcoI site the second amino acid of χ was changed from Lys to Ala. After cloning the gene was completely sequenced and no further mutations were detected.

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  • 78
    Stratagene escherichia coli strains jm109
    Escherichia Coli Strains Jm109, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strains jm109/product/Stratagene
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strains jm109 - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    82
    Stratagene e coli strain jm109
    E Coli Strain Jm109, supplied by Stratagene, used in various techniques. Bioz Stars score: 82/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain jm109/product/Stratagene
    Average 82 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    e coli strain jm109 - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

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