escherichia coli strains jm109  (Millipore)


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    Structured Review

    Millipore escherichia coli strains jm109
    Escherichia Coli Strains Jm109, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strains jm109/product/Millipore
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strains jm109 - by Bioz Stars, 2020-01
    77/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: Paragraph title: Cloning and expression studies of mouse Nat2 ... The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford).

    Article Title: QscR, a LysR-Type Transcriptional Regulator and CbbR Homolog, Is Involved in Regulation of the Serine Cycle Genes in Methylobacterium extorquens AM1
    Article Snippet: Escherichia coli strains JM109 , BL21(DE3) (Novagen, Madison, Wis.), S17-1 , and Top 10 (Invitrogen) were routinely cultivated at 37°C in Luria-Bertani (LB) medium ( ). .. The following cloning vectors were used: pUC19 (Pharmacia) for cloning and subcloning, pAYC61 ( ) as a suicide vector, pRK2013 ( ) as a helper plasmid, pCR2.1 (Invitrogen) for cloning of PCR products, and pCM130 ( ) for promoter fusion construction.

    Article Title: Mechanism-based site-directed mutagenesis to shift the optimum pH of the phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1
    Article Snippet: 2.1 Plasmids and strains The plasmids pMD18-T (Takara, Japan) and pET-28a (+) (Novagen, USA) were used for cloning and expression. .. The E. coli strains JM109 and BL21 (DE3) (Novagen, USA) were used as a host strains for plasmid amplification and enzyme expression, respectively.

    Article Title: An autonomous folding unit mediates the assembly of two-stranded coiled coils
    Article Snippet: The recombinant fragments GCN4p-wt, GCN4p-Cort, GCN4p-Cort T/L, GCN4p-c1, and GCN4p-c2 were expressed in Escherichia coli strain JM109(DE3) (Novagen). .. DNA manipulations for cloning were performed according to standard protocols ( ).

    Functional Assay:

    Article Title: Tuning the Specificity of the Recombinant Multicomponent Toluene o-Xylene Monooxygenase from Pseudomonas sp. Strain OX1 for the Biosynthesis of Tyrosol from 2-Phenylethanol ▿ sp. Strain OX1 for the Biosynthesis of Tyrosol from 2-Phenylethanol ▿ †
    Article Snippet: Escherichia coli strain JM109 was from Novagen. .. Plasmid pTou was kindly supplied by Valeria Cafaro (Department of Structural and Functional Biology, University Federico II, Naples, Italy).

    Positron Emission Tomography:

    Article Title: Mechanism-based site-directed mutagenesis to shift the optimum pH of the phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1
    Article Snippet: The pET-28a-pal that encodes the Rg PAL gene from R. glutinis JN-1 (CCTCC M2011490) was constructed in our previous study . .. The E. coli strains JM109 and BL21 (DE3) (Novagen, USA) were used as a host strains for plasmid amplification and enzyme expression, respectively.

    Article Title: Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation
    Article Snippet: .. E. coli strains JM109 and XL1 were used for molecular cloning experiments to generate pET vector- or pGEM-T-based constructs, and E. coli BL21/λDE3 (Novagen) for expressing recombinant proteins. .. E. coli was cultured in LB broth at 37°C with shaking.

    Agarose Gel Electrophoresis:

    Article Title: Regiospecificity of Two Multicomponent Monooxygenases from Pseudomonas stutzeri OX1: Molecular Basis for Catabolic Adaptation of This Microorganism to Methylated Aromatic Compounds
    Article Snippet: E. coli strain JM109 was obtained from Novagen. .. The pGEM-3Z expression vector and Wizard SV gel and PCR clean-up system for elution of DNA fragments from the agarose gel were obtained from Promega.

    Molecular Cloning:

    Article Title: Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation
    Article Snippet: .. E. coli strains JM109 and XL1 were used for molecular cloning experiments to generate pET vector- or pGEM-T-based constructs, and E. coli BL21/λDE3 (Novagen) for expressing recombinant proteins. .. E. coli was cultured in LB broth at 37°C with shaking.

    Mutagenesis:

    Article Title: QscR, a LysR-Type Transcriptional Regulator and CbbR Homolog, Is Involved in Regulation of the Serine Cycle Genes in Methylobacterium extorquens AM1
    Article Snippet: For serine cycle enzyme induction in mutant strains unable to grow on C1 compounds, succinate-grown cells were pelleted, washed, and exposed to methanol at 30°C with shaking for 16 to 18 h. M. extorquens CM82.1 (C. J. Marx, unpublished results) was grow on methanol or succinate in the previously described medium with addition KAN (100 μg ml−1 ). .. Escherichia coli strains JM109 , BL21(DE3) (Novagen, Madison, Wis.), S17-1 , and Top 10 (Invitrogen) were routinely cultivated at 37°C in Luria-Bertani (LB) medium ( ).

    Subcloning:

    Article Title: QscR, a LysR-Type Transcriptional Regulator and CbbR Homolog, Is Involved in Regulation of the Serine Cycle Genes in Methylobacterium extorquens AM1
    Article Snippet: Escherichia coli strains JM109 , BL21(DE3) (Novagen, Madison, Wis.), S17-1 , and Top 10 (Invitrogen) were routinely cultivated at 37°C in Luria-Bertani (LB) medium ( ). .. The following cloning vectors were used: pUC19 (Pharmacia) for cloning and subcloning, pAYC61 ( ) as a suicide vector, pRK2013 ( ) as a helper plasmid, pCR2.1 (Invitrogen) for cloning of PCR products, and pCM130 ( ) for promoter fusion construction.

    Construct:

    Article Title: Mechanism-based site-directed mutagenesis to shift the optimum pH of the phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1
    Article Snippet: The pET-28a-pal that encodes the Rg PAL gene from R. glutinis JN-1 (CCTCC M2011490) was constructed in our previous study . .. The E. coli strains JM109 and BL21 (DE3) (Novagen, USA) were used as a host strains for plasmid amplification and enzyme expression, respectively.

    Article Title: Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation
    Article Snippet: .. E. coli strains JM109 and XL1 were used for molecular cloning experiments to generate pET vector- or pGEM-T-based constructs, and E. coli BL21/λDE3 (Novagen) for expressing recombinant proteins. .. E. coli was cultured in LB broth at 37°C with shaking.

    Purification:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: This allowed the production of recombinant mouse NAT protein with an N-terminal His-tag, for ease of downstream purification. .. The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford).

    Article Title: Regiospecificity of Two Multicomponent Monooxygenases from Pseudomonas stutzeri OX1: Molecular Basis for Catabolic Adaptation of This Microorganism to Methylated Aromatic Compounds
    Article Snippet: Bacterial culturing, plasmid purification, and transformation were performed as described by Sambrook et al. ( ). .. E. coli strain JM109 was obtained from Novagen.

    Plasmid Preparation:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: .. The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford). ..

    Article Title: QscR, a LysR-Type Transcriptional Regulator and CbbR Homolog, Is Involved in Regulation of the Serine Cycle Genes in Methylobacterium extorquens AM1
    Article Snippet: Escherichia coli strains JM109 , BL21(DE3) (Novagen, Madison, Wis.), S17-1 , and Top 10 (Invitrogen) were routinely cultivated at 37°C in Luria-Bertani (LB) medium ( ). .. The following cloning vectors were used: pUC19 (Pharmacia) for cloning and subcloning, pAYC61 ( ) as a suicide vector, pRK2013 ( ) as a helper plasmid, pCR2.1 (Invitrogen) for cloning of PCR products, and pCM130 ( ) for promoter fusion construction.

    Article Title: Tuning the Specificity of the Recombinant Multicomponent Toluene o-Xylene Monooxygenase from Pseudomonas sp. Strain OX1 for the Biosynthesis of Tyrosol from 2-Phenylethanol ▿ sp. Strain OX1 for the Biosynthesis of Tyrosol from 2-Phenylethanol ▿ †
    Article Snippet: Bacterial cultures, plasmid purifications, and transformations were performed according to Sambrook et al ( ). .. Escherichia coli strain JM109 was from Novagen.

    Article Title: Mechanism-based site-directed mutagenesis to shift the optimum pH of the phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1
    Article Snippet: .. The E. coli strains JM109 and BL21 (DE3) (Novagen, USA) were used as a host strains for plasmid amplification and enzyme expression, respectively. ..

    Article Title: Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation
    Article Snippet: .. E. coli strains JM109 and XL1 were used for molecular cloning experiments to generate pET vector- or pGEM-T-based constructs, and E. coli BL21/λDE3 (Novagen) for expressing recombinant proteins. .. E. coli was cultured in LB broth at 37°C with shaking.

    Article Title: Regiospecificity of Two Multicomponent Monooxygenases from Pseudomonas stutzeri OX1: Molecular Basis for Catabolic Adaptation of This Microorganism to Methylated Aromatic Compounds
    Article Snippet: Bacterial culturing, plasmid purification, and transformation were performed as described by Sambrook et al. ( ). .. E. coli strain JM109 was obtained from Novagen.

    Sequencing:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: .. The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford). ..

    Incubation:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford). .. Thawed glycerol stock (100 μL) was used to inoculate fresh LB media (100 mL) supplemented with kanamycin (30 μg/mL) and chloramphenicol (34 μg/mL) and the culture was incubated at 37 °C for 16 h with shaking (180 rpm).

    Amplification:

    Article Title: Mechanism-based site-directed mutagenesis to shift the optimum pH of the phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1
    Article Snippet: .. The E. coli strains JM109 and BL21 (DE3) (Novagen, USA) were used as a host strains for plasmid amplification and enzyme expression, respectively. ..

    Cell Culture:

    Article Title: Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation
    Article Snippet: E. coli strains JM109 and XL1 were used for molecular cloning experiments to generate pET vector- or pGEM-T-based constructs, and E. coli BL21/λDE3 (Novagen) for expressing recombinant proteins. .. E. coli was cultured in LB broth at 37°C with shaking.

    Expressing:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: Paragraph title: Cloning and expression studies of mouse Nat2 ... The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford).

    Article Title: Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal
    Article Snippet: .. E. coli strain JM109 was used for DNA manipulations, and BL21(DE3) (Novagen) was used for the expression of the maltose binding protein (MBP) fusion of Pop2p. .. The transformation of yeast was performed by the LiOAc-method ( ).

    Article Title: Tuning the Specificity of the Recombinant Multicomponent Toluene o-Xylene Monooxygenase from Pseudomonas sp. Strain OX1 for the Biosynthesis of Tyrosol from 2-Phenylethanol ▿ sp. Strain OX1 for the Biosynthesis of Tyrosol from 2-Phenylethanol ▿ †
    Article Snippet: Escherichia coli strain JM109 was from Novagen. .. The pGEM3Z expression vector, Wizard SV gel, and the PCR cleanup system for the elution of DNA fragments from agarose gels were obtained from Promega.

    Article Title: Mechanism-based site-directed mutagenesis to shift the optimum pH of the phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1
    Article Snippet: .. The E. coli strains JM109 and BL21 (DE3) (Novagen, USA) were used as a host strains for plasmid amplification and enzyme expression, respectively. ..

    Article Title: Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation
    Article Snippet: .. E. coli strains JM109 and XL1 were used for molecular cloning experiments to generate pET vector- or pGEM-T-based constructs, and E. coli BL21/λDE3 (Novagen) for expressing recombinant proteins. .. E. coli was cultured in LB broth at 37°C with shaking.

    Article Title: Regiospecificity of Two Multicomponent Monooxygenases from Pseudomonas stutzeri OX1: Molecular Basis for Catabolic Adaptation of This Microorganism to Methylated Aromatic Compounds
    Article Snippet: E. coli strain JM109 was obtained from Novagen. .. The pGEM-3Z expression vector and Wizard SV gel and PCR clean-up system for elution of DNA fragments from the agarose gel were obtained from Promega.

    Polymerase Chain Reaction:

    Article Title: QscR, a LysR-Type Transcriptional Regulator and CbbR Homolog, Is Involved in Regulation of the Serine Cycle Genes in Methylobacterium extorquens AM1
    Article Snippet: Escherichia coli strains JM109 , BL21(DE3) (Novagen, Madison, Wis.), S17-1 , and Top 10 (Invitrogen) were routinely cultivated at 37°C in Luria-Bertani (LB) medium ( ). .. The following cloning vectors were used: pUC19 (Pharmacia) for cloning and subcloning, pAYC61 ( ) as a suicide vector, pRK2013 ( ) as a helper plasmid, pCR2.1 (Invitrogen) for cloning of PCR products, and pCM130 ( ) for promoter fusion construction.

    Article Title: Tuning the Specificity of the Recombinant Multicomponent Toluene o-Xylene Monooxygenase from Pseudomonas sp. Strain OX1 for the Biosynthesis of Tyrosol from 2-Phenylethanol ▿ sp. Strain OX1 for the Biosynthesis of Tyrosol from 2-Phenylethanol ▿ †
    Article Snippet: Escherichia coli strain JM109 was from Novagen. .. The pGEM3Z expression vector, Wizard SV gel, and the PCR cleanup system for the elution of DNA fragments from agarose gels were obtained from Promega.

    Article Title: Regiospecificity of Two Multicomponent Monooxygenases from Pseudomonas stutzeri OX1: Molecular Basis for Catabolic Adaptation of This Microorganism to Methylated Aromatic Compounds
    Article Snippet: E. coli strain JM109 was obtained from Novagen. .. The pGEM-3Z expression vector and Wizard SV gel and PCR clean-up system for elution of DNA fragments from the agarose gel were obtained from Promega.

    Recombinant:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: This allowed the production of recombinant mouse NAT protein with an N-terminal His-tag, for ease of downstream purification. .. The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford).

    Article Title: An autonomous folding unit mediates the assembly of two-stranded coiled coils
    Article Snippet: .. The recombinant fragments GCN4p-wt, GCN4p-Cort, GCN4p-Cort T/L, GCN4p-c1, and GCN4p-c2 were expressed in Escherichia coli strain JM109(DE3) (Novagen). ..

    Article Title: Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation
    Article Snippet: .. E. coli strains JM109 and XL1 were used for molecular cloning experiments to generate pET vector- or pGEM-T-based constructs, and E. coli BL21/λDE3 (Novagen) for expressing recombinant proteins. .. E. coli was cultured in LB broth at 37°C with shaking.

    Transformation Assay:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: .. The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford). ..

    Article Title: Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal
    Article Snippet: E. coli strain JM109 was used for DNA manipulations, and BL21(DE3) (Novagen) was used for the expression of the maltose binding protein (MBP) fusion of Pop2p. .. The transformation of yeast was performed by the LiOAc-method ( ).

    Article Title: Regiospecificity of Two Multicomponent Monooxygenases from Pseudomonas stutzeri OX1: Molecular Basis for Catabolic Adaptation of This Microorganism to Methylated Aromatic Compounds
    Article Snippet: Bacterial culturing, plasmid purification, and transformation were performed as described by Sambrook et al. ( ). .. E. coli strain JM109 was obtained from Novagen.

    Binding Assay:

    Article Title: Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal
    Article Snippet: .. E. coli strain JM109 was used for DNA manipulations, and BL21(DE3) (Novagen) was used for the expression of the maltose binding protein (MBP) fusion of Pop2p. .. The transformation of yeast was performed by the LiOAc-method ( ).

    Derivative Assay:

    Article Title: Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal
    Article Snippet: All S. cerevisiae strains used in this study were derived from S288C and are listed in Table . .. E. coli strain JM109 was used for DNA manipulations, and BL21(DE3) (Novagen) was used for the expression of the maltose binding protein (MBP) fusion of Pop2p.

    DNA Sequencing:

    Article Title: Mouse N-acetyltransferase type 2, the homologue of human N-acetyltransferase type 1
    Article Snippet: .. The pET28b(+) plasmid containing mouse Nat2 was transformed into Escherichia coli strain JM109 and further transformed into Rosetta(DE3)pLysS (Novagen) after confirming the correct insert sequence (DNA Sequencing Facility, Biochemistry, University of Oxford). ..

    Article Title: An autonomous folding unit mediates the assembly of two-stranded coiled coils
    Article Snippet: The recombinant fragments GCN4p-wt, GCN4p-Cort, GCN4p-Cort T/L, GCN4p-c1, and GCN4p-c2 were expressed in Escherichia coli strain JM109(DE3) (Novagen). .. Recombinant insert DNA was verified by Sanger dideoxy DNA sequencing.

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  • 89
    Millipore e coli jm109
    E Coli Jm109, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli jm109/product/Millipore
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    e coli jm109 - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

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