escherichia coli strains dh5α  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89

    Structured Review

    Millipore escherichia coli strains dh5α
    Escherichia Coli Strains Dh5α, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strains dh5α/product/Millipore
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strains dh5α - by Bioz Stars, 2020-01
    89/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: Paragraph title: Cloning and expression of human haptoglobin β subunit ... The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml).

    Article Title: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo
    Article Snippet: pMD20-T (Takara, Japan) was used for gene cloning. pET-21b (Novagen, USA) was employed to construct expression vector. .. E. coli strain DH5α (Novagen, USA) was applied as the host for gene manipulation.

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: Paragraph title: Cloning and expression of Cel10   ... Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing.

    Article Title: Identification of Ser/Thr kinase and Forkhead Associated Domains in Mycobacterium ulcerans: Characterization of Novel Association between Protein Kinase Q and MupFHA
    Article Snippet: All of the Gromacs MD simulations were run in the HPC-Supercomputer facility (CSIR-IGIB, India) on 32 Cores at 11.4 ns/day maximum performance speed. .. Escherichia coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) for the expression of recombinant proteins. .. E. coli cells were grown and maintained with constant shaking (200 rpm) at 37°C in LB broth supplemented with appropriate antibiotic (100 µg/ml ampicillin and/or 12.5 µg/ml chloramphenicol), when needed.

    Article Title: Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana
    Article Snippet: The PCR product was cloned into the plasmid pGEX-4T1 (GE Healthcare) that encodes an upstream glutathione S -transferase (GST) tag followed by a thrombin cleavage site. .. Escherichia coli strain DH5α (Novagen) was used for plasmid cloning, and the insertion of the gene for the APR1 glutaredoxin domain was confirmed by DNA sequencing. .. The recombinant plasmid was transformed into E. coli strain BL21(DE3) (Novagen) for protein expression.

    Article Title: Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis
    Article Snippet: Future studies will be needed to define role of GroEL in biofilm formation and cell surface attachment in B. anthracis . .. E. coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) was used for the expression of recombinant proteins. .. E. coli cells were grown as described before., B. anthracis Sterne strain (wild-type Bas -wt and Bas ΔprkC ), and B. subtilis were grown in LB broth at 37 °C with shaking at 200 rpm.

    Article Title: Construction of a Novel Liver-Targeting Fusion Interferon by Incorporation of a Plasmodium Region I-Plus Peptide
    Article Snippet: We further investigated whether the fusion protein IFN-CSP reserves anti-HBV activity and liver-targeting function as its parental peptides. .. E. coli strain DH5α (Novagen, USA) and BL21 (DE3; Novagen, USA) were used as the host for gene manipulation and expression of fusion protein, respectively. pMD20-T (Takara, Japan) and pET-21b (Novagen, USA) were applied for gene cloning and expression, respectively. .. Luria-Bertani (LB) medium was employed for bacterial growth and protein production.

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells
    Article Snippet: S. enterica subsp. enteric serovar typhimurium (ATCC 14028) was obtained from the American Type Culture Collection (ATCC, Manassas, VA., USA) and cultured on Salmonella Shigella agar medium (Oxoid CM0099, UK ) and Neutrient broth (Himedia, India) at 37ºC and 5% CO2 . .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pPrimecloning vector (5PRIME Inc. Germany) was used for cloning the PCR products, while pVAX1 (Invitrogen®, Carlsbad, CA, USA) was used for sub-cloning the fliC gene. .. Eukaryotic expression vector pVAX1 carries the human cytomegalovirus (CMV) immediate early promoter, the bovine growth hormone (BGH) polyadenylation signal for transcription termination, the kanamycin resistance gene and the pUC origin of replication for maintenance in E. coli .

    Article Title: Genetic and Mass Spectrometry Analyses of the Unusual Type IV-Like Pili of the Archaeon Methanococcus maripaludis
    Article Snippet: In complementation experiments, the plasmids were maintained in M. maripaludis by puromycin (2.5 μg/ml) selection. .. Escherichia coli strain DH5α (Novagen) was used for intermediate cloning steps. .. Cells were grown at 37°C in Luria-Bertani medium supplemented with ampicillin (100 μg/ml) when necessary.

    Article Title: Understanding the Role of PknJ in Mycobacterium tuberculosis: Biochemical Characterization and Identification of Novel Substrate Pyruvate Kinase A
    Article Snippet: Ser37 is the influential residue for mtPykA which aids in ATP and pyruvate generation and is also identified as one of the phosphorylation site. .. E. coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) for the expression of recombinant proteins. .. E. coli cells were grown and maintained with constant shaking (220 rpm) at 37°C in LB medium supplemented with 100 µg/ml ampicillin and/or 40 µg/ml kanamycin, when needed.

    Article Title: Cloning and Expression of Major Surface Antigen 1 Gene of Toxoplasma gondii RH Strain Using the Expression Vector pVAX1 in Chinese Hamster Ovary Cells
    Article Snippet: The polymerase chain reaction (PCR) cloning Kit was purchased from 5prime (Germany) and other kits were purchased from Qiagen (Germany), restriction endonucleases were purchased from New England Biolabs (New England Biolabs Inc., USA). .. The Escherichia coli strain DH5α and pVAX1 expression vector were purchased from Novagen (Novagen Inc., Madison, WI, USA).

    Amplification:

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: The PCR product was cloned into the expression vector pET-32a [modified by inserting a Tobacco etch virus (TEV) protease cleavage site inside the NcoI site] with BamHI and XhoI. .. Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing. .. The recombinant plasmid was then transformed into E. coli strain BL21 (DE3) (Novagen) for protein expression.

    Article Title: Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution
    Article Snippet: Based in the recently solved crystal structure of the homodimeric wild type enzyme we rationalize the molecular basis behind the increased kinetic or thermodynamic stability of PpAzoR enzyme. .. Escherichia coli strain DH5α (Novagen) was used for routine propagation and amplification of plasmid constructs. .. E. coli Tuner (DE3, Novagen) and KRX (Promega) strains were used to express the ppAzoR and variant genes cloned in pET-21a (+) plasmid (Novagen).

    Article Title: Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana
    Article Snippet: The DNA encoding amino acids 353–461 of A. thaliana APR1 (465 amino acids, TAIR accession No. At4G04610.1) was amplified by PCR using a prepared cDNA library and the gene-specific forward and reverse primers listed in Table 1 . .. Escherichia coli strain DH5α (Novagen) was used for plasmid cloning, and the insertion of the gene for the APR1 glutaredoxin domain was confirmed by DNA sequencing.

    Expressing:

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: Paragraph title: Cloning and expression of human haptoglobin β subunit ... The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml).

    Article Title: Design, Construction and Cloning of Truncated ORF2 and tPAsp-PADRE-Truncated ORF2 Gene Cassette From Hepatitis E Virus in the pVAX1 Expression Vector
    Article Snippet: Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pVAX1 plasmid (Invitrogen® , Carls-bad, CA, USA) was used for subcloning of the truncated ORF2 and tPAsp-PADRE-truncated ORF2 (112 - 660 aa) gene cassette. .. Eukaryotic expression vector, pVAX1, was constructed to be consistent with the Food and Drug Administration (FDA) regulations.

    Article Title: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo
    Article Snippet: pMD20-T (Takara, Japan) was used for gene cloning. pET-21b (Novagen, USA) was employed to construct expression vector. .. E. coli strain DH5α (Novagen, USA) was applied as the host for gene manipulation.

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: Paragraph title: Cloning and expression of Cel10   ... Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing.

    Article Title: Identification of Ser/Thr kinase and Forkhead Associated Domains in Mycobacterium ulcerans: Characterization of Novel Association between Protein Kinase Q and MupFHA
    Article Snippet: All of the Gromacs MD simulations were run in the HPC-Supercomputer facility (CSIR-IGIB, India) on 32 Cores at 11.4 ns/day maximum performance speed. .. Escherichia coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) for the expression of recombinant proteins. .. E. coli cells were grown and maintained with constant shaking (200 rpm) at 37°C in LB broth supplemented with appropriate antibiotic (100 µg/ml ampicillin and/or 12.5 µg/ml chloramphenicol), when needed.

    Article Title: Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
    Article Snippet: pMD20-T (Takara, Otsu, Japan) was employed to clone gene and pET-21b (Novagen, Madison, USA) was employed to construct expression plasmid. .. E. coli strain DH5α (Novagen, Madison, USA) was employed for gene manipulation and BL21 (DE3; Novagen, Madison, USA) was employed to construct recombinant expression strain. .. Recombinant IFN-CSP/pET-21b expression plasmid was obtained from Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou, People’s Republic of China.

    Article Title: Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis
    Article Snippet: Future studies will be needed to define role of GroEL in biofilm formation and cell surface attachment in B. anthracis . .. E. coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) was used for the expression of recombinant proteins. .. E. coli cells were grown as described before., B. anthracis Sterne strain (wild-type Bas -wt and Bas ΔprkC ), and B. subtilis were grown in LB broth at 37 °C with shaking at 200 rpm.

    Article Title: W8, a new Sup35 prion strain, transmits distinctive information with a conserved assembly scheme
    Article Snippet: Standard protocols were used for media preparation and yeast genetic manipulation. .. Escherichia coli strains DH5α and BLR(DE3)pLysS (Novagen) were used for plasmid construction and protein expression, respectively. .. White, pink and dark pink colonies were selected for strain typing.

    Article Title: Construction of a Novel Liver-Targeting Fusion Interferon by Incorporation of a Plasmodium Region I-Plus Peptide
    Article Snippet: We further investigated whether the fusion protein IFN-CSP reserves anti-HBV activity and liver-targeting function as its parental peptides. .. E. coli strain DH5α (Novagen, USA) and BL21 (DE3; Novagen, USA) were used as the host for gene manipulation and expression of fusion protein, respectively. pMD20-T (Takara, Japan) and pET-21b (Novagen, USA) were applied for gene cloning and expression, respectively. .. Luria-Bertani (LB) medium was employed for bacterial growth and protein production.

    Article Title: Understanding the Role of PknJ in Mycobacterium tuberculosis: Biochemical Characterization and Identification of Novel Substrate Pyruvate Kinase A
    Article Snippet: Ser37 is the influential residue for mtPykA which aids in ATP and pyruvate generation and is also identified as one of the phosphorylation site. .. E. coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) for the expression of recombinant proteins. .. E. coli cells were grown and maintained with constant shaking (220 rpm) at 37°C in LB medium supplemented with 100 µg/ml ampicillin and/or 40 µg/ml kanamycin, when needed.

    Article Title: Cloning and Expression of Major Surface Antigen 1 Gene of Toxoplasma gondii RH Strain Using the Expression Vector pVAX1 in Chinese Hamster Ovary Cells
    Article Snippet: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal) and Isopropyl-β-D-thiogalactopyranoside (IPTG) were purchased from Sigma-Aldrich Corporation (Germany). .. The Escherichia coli strain DH5α and pVAX1 expression vector were purchased from Novagen (Novagen Inc., Madison, WI, USA). .. All chemicals were obtained from Merck (Germany), Sigma-Aldrich (Germany) and HiMedia (India) Corporations.

    Synthesized:

    Article Title: A strategy to identify a ketoreductase that preferentially synthesizes pharmaceutically relevant (S)-alcohols using whole-cell biotransformation
    Article Snippet: Escherichia coli strains DH5α (Novagen, U SA) and BL21(DE3) (Novagen, USA) are used as hosts for sub-cloning and over-expression respectively. .. Escherichia coli strains DH5α (Novagen, U SA) and BL21(DE3) (Novagen, USA) are used as hosts for sub-cloning and over-expression respectively.

    Construct:

    Article Title: Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases
    Article Snippet: The resulting plasmids (p11α-SHAoch and pCYP509C12, respectively) were transformed into E. coli strain DH5α (Novagen) that were selected and maintained at 37 °C using Luria–Bertani medium supplemented with sodium ampicillin (100 mg/L). .. Purified p11α-SHAoch and pCYP509C12 plasmid DNA were isolated from E. coli cultures (Wizard Plus SV Minipreps, Promega) and transformed into S. cerevisiae strain AH22 (MAT a, leu2 -3 , 112 his4 -519 can1 ) via electroporation to yield the experimental constructs AH22:p11α-SHAoch and AH22:pCYP509C12.

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: The expression plasmid construct encoding human haptoglobin (accession number ) β subunit (245 amino acids corresponding to 162-406 residues of haptoglobin holo-protein) was made available by GeneCopoeia Inc., (Germantown, MD). .. The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml).

    Article Title: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo
    Article Snippet: pMD20-T (Takara, Japan) was used for gene cloning. pET-21b (Novagen, USA) was employed to construct expression vector. .. E. coli strain DH5α (Novagen, USA) was applied as the host for gene manipulation.

    Article Title: Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution
    Article Snippet: Based in the recently solved crystal structure of the homodimeric wild type enzyme we rationalize the molecular basis behind the increased kinetic or thermodynamic stability of PpAzoR enzyme. .. Escherichia coli strain DH5α (Novagen) was used for routine propagation and amplification of plasmid constructs. .. E. coli Tuner (DE3, Novagen) and KRX (Promega) strains were used to express the ppAzoR and variant genes cloned in pET-21a (+) plasmid (Novagen).

    Article Title: Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
    Article Snippet: pMD20-T (Takara, Otsu, Japan) was employed to clone gene and pET-21b (Novagen, Madison, USA) was employed to construct expression plasmid. .. E. coli strain DH5α (Novagen, Madison, USA) was employed for gene manipulation and BL21 (DE3; Novagen, Madison, USA) was employed to construct recombinant expression strain. .. Recombinant IFN-CSP/pET-21b expression plasmid was obtained from Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou, People’s Republic of China.

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells
    Article Snippet: Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pPrimecloning vector (5PRIME Inc. Germany) was used for cloning the PCR products, while pVAX1 (Invitrogen®, Carlsbad, CA, USA) was used for sub-cloning the fliC gene. .. Eukaryotic expression vector pVAX1 carries the human cytomegalovirus (CMV) immediate early promoter, the bovine growth hormone (BGH) polyadenylation signal for transcription termination, the kanamycin resistance gene and the pUC origin of replication for maintenance in E. coli .

    Incubation:

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: The correct DNA insert (757 bp) was confirmed by digesting the plasmid with restriction enzymes XmnI and NotI. .. The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml). .. Fusion protein was isolated by affinity purification using the Nickel-charged 6 X histidine binding resin beads (Novagen).

    Mass Spectrometry:

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml). .. The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml).

    Modification:

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: The PCR product was cloned into the expression vector pET-32a [modified by inserting a Tobacco etch virus (TEV) protease cleavage site inside the NcoI site] with BamHI and XhoI. .. Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing.

    Transformation Assay:

    Article Title: Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases
    Article Snippet: Optimized genes for A. ochraceus and R. oryzae were excised from pEX-K and ligated into pre-cut (Bam HI, Xho I) p425TEF vector. .. The resulting plasmids (p11α-SHAoch and pCYP509C12, respectively) were transformed into E. coli strain DH5α (Novagen) that were selected and maintained at 37 °C using Luria–Bertani medium supplemented with sodium ampicillin (100 mg/L). .. Purified p11α-SHAoch and pCYP509C12 plasmid DNA were isolated from E. coli cultures (Wizard Plus SV Minipreps, Promega) and transformed into S. cerevisiae strain AH22 (MAT a, leu2 -3 , 112 his4 -519 can1 ) via electroporation to yield the experimental constructs AH22:p11α-SHAoch and AH22:pCYP509C12.

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: The correct DNA insert (757 bp) was confirmed by digesting the plasmid with restriction enzymes XmnI and NotI. .. The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml). .. Fusion protein was isolated by affinity purification using the Nickel-charged 6 X histidine binding resin beads (Novagen).

    Article Title: Design, Construction and Cloning of Truncated ORF2 and tPAsp-PADRE-Truncated ORF2 Gene Cassette From Hepatitis E Virus in the pVAX1 Expression Vector
    Article Snippet: Furthermore, the main goal of the present study was to construct the recombinant plasmids pVAX-tPAsp -PADRE-truncated ORF2 and pVAX-truncated ORF2 as DNA vaccine candidates. .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pVAX1 plasmid (Invitrogen® , Carls-bad, CA, USA) was used for subcloning of the truncated ORF2 and tPAsp-PADRE-truncated ORF2 (112 - 660 aa) gene cassette. .. Eukaryotic expression vector, pVAX1, was constructed to be consistent with the Food and Drug Administration (FDA) regulations.

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells
    Article Snippet: S. enterica subsp. enteric serovar typhimurium (ATCC 14028) was obtained from the American Type Culture Collection (ATCC, Manassas, VA., USA) and cultured on Salmonella Shigella agar medium (Oxoid CM0099, UK ) and Neutrient broth (Himedia, India) at 37ºC and 5% CO2 . .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pPrimecloning vector (5PRIME Inc. Germany) was used for cloning the PCR products, while pVAX1 (Invitrogen®, Carlsbad, CA, USA) was used for sub-cloning the fliC gene. .. Eukaryotic expression vector pVAX1 carries the human cytomegalovirus (CMV) immediate early promoter, the bovine growth hormone (BGH) polyadenylation signal for transcription termination, the kanamycin resistance gene and the pUC origin of replication for maintenance in E. coli .

    Article Title: Genetic and Mass Spectrometry Analyses of the Unusual Type IV-Like Pili of the Archaeon Methanococcus maripaludis
    Article Snippet: For the in-frame deletion mutagenesis experiments with M. maripaludis Mm900, cells were grown in McCas medium supplemented with 8-azahypoxanthine (240 μg/ml) or neomycin (1 mg/ml) as required at various steps in the transformation procedure outlined by Moore and Leigh ( ). .. Escherichia coli strain DH5α (Novagen) was used for intermediate cloning steps.

    Over Expression:

    Article Title: A strategy to identify a ketoreductase that preferentially synthesizes pharmaceutically relevant (S)-alcohols using whole-cell biotransformation
    Article Snippet: This understanding of the catalytic mechanism and cofactor augmentation can be extended to other ketoreductases and substrates to reduce the enzyme screening space and enable manufacture of high value chiral alcohols. .. Escherichia coli strains DH5α (Novagen, U SA) and BL21(DE3) (Novagen, USA) are used as hosts for sub-cloning and over-expression respectively. .. Restriction enzymes NdeI and XhoI are purchased from NEB (New England Biolabs).

    Article Title: SCO5745, a Bifunctional RNase J Ortholog, Affects Antibiotic Production in Streptomyces coelicolor
    Article Snippet: We also demonstrate that sco5745 is not essential and that disruption of the gene affects antibiotic production in the disrupted strain. .. Escherichia coli strains DH5α and Rosetta 2(DE3)pLysS (Novagen) were used as hosts for plasmid manipulation and protein overexpression, respectively. .. E. coli strains BW25113 and ET12567 ( dam dcm hsdS ) were used for gene disruption and conjugation purposes ( ).

    Derivative Assay:

    Article Title: Genetic and Mass Spectrometry Analyses of the Unusual Type IV-Like Pili of the Archaeon Methanococcus maripaludis
    Article Snippet: M. maripaludis Δ hpt (Mm900) ( ) and Δ flaK ( ) mutants and all of the subsequent mutants derived from them were routinely grown in Balch medium III ( ) as previously described ( ). .. Escherichia coli strain DH5α (Novagen) was used for intermediate cloning steps.

    Cell Culture:

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells
    Article Snippet: S. enterica subsp. enteric serovar typhimurium (ATCC 14028) was obtained from the American Type Culture Collection (ATCC, Manassas, VA., USA) and cultured on Salmonella Shigella agar medium (Oxoid CM0099, UK ) and Neutrient broth (Himedia, India) at 37ºC and 5% CO2 . .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pPrimecloning vector (5PRIME Inc. Germany) was used for cloning the PCR products, while pVAX1 (Invitrogen®, Carlsbad, CA, USA) was used for sub-cloning the fliC gene.

    DNA Sequencing:

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: The PCR product was cloned into the expression vector pET-32a [modified by inserting a Tobacco etch virus (TEV) protease cleavage site inside the NcoI site] with BamHI and XhoI. .. Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing. .. The recombinant plasmid was then transformed into E. coli strain BL21 (DE3) (Novagen) for protein expression.

    Article Title: Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana
    Article Snippet: The PCR product was cloned into the plasmid pGEX-4T1 (GE Healthcare) that encodes an upstream glutathione S -transferase (GST) tag followed by a thrombin cleavage site. .. Escherichia coli strain DH5α (Novagen) was used for plasmid cloning, and the insertion of the gene for the APR1 glutaredoxin domain was confirmed by DNA sequencing. .. The recombinant plasmid was transformed into E. coli strain BL21(DE3) (Novagen) for protein expression.

    Polymerase Chain Reaction:

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: The PCR product was cloned into the expression vector pET-32a [modified by inserting a Tobacco etch virus (TEV) protease cleavage site inside the NcoI site] with BamHI and XhoI. .. Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing.

    Article Title: Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana
    Article Snippet: The PCR product was cloned into the plasmid pGEX-4T1 (GE Healthcare) that encodes an upstream glutathione S -transferase (GST) tag followed by a thrombin cleavage site. .. Escherichia coli strain DH5α (Novagen) was used for plasmid cloning, and the insertion of the gene for the APR1 glutaredoxin domain was confirmed by DNA sequencing.

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells
    Article Snippet: S. enterica subsp. enteric serovar typhimurium (ATCC 14028) was obtained from the American Type Culture Collection (ATCC, Manassas, VA., USA) and cultured on Salmonella Shigella agar medium (Oxoid CM0099, UK ) and Neutrient broth (Himedia, India) at 37ºC and 5% CO2 . .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pPrimecloning vector (5PRIME Inc. Germany) was used for cloning the PCR products, while pVAX1 (Invitrogen®, Carlsbad, CA, USA) was used for sub-cloning the fliC gene. .. Eukaryotic expression vector pVAX1 carries the human cytomegalovirus (CMV) immediate early promoter, the bovine growth hormone (BGH) polyadenylation signal for transcription termination, the kanamycin resistance gene and the pUC origin of replication for maintenance in E. coli .

    Article Title: Cloning and Expression of Major Surface Antigen 1 Gene of Toxoplasma gondii RH Strain Using the Expression Vector pVAX1 in Chinese Hamster Ovary Cells
    Article Snippet: The polymerase chain reaction (PCR) cloning Kit was purchased from 5prime (Germany) and other kits were purchased from Qiagen (Germany), restriction endonucleases were purchased from New England Biolabs (New England Biolabs Inc., USA). .. The Escherichia coli strain DH5α and pVAX1 expression vector were purchased from Novagen (Novagen Inc., Madison, WI, USA).

    Recombinant:

    Article Title: Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases
    Article Snippet: Paragraph title: Plasmid construction and recombinant yeast ... The resulting plasmids (p11α-SHAoch and pCYP509C12, respectively) were transformed into E. coli strain DH5α (Novagen) that were selected and maintained at 37 °C using Luria–Bertani medium supplemented with sodium ampicillin (100 mg/L).

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: The recombinant plasmid is tagged with 6 × histidine at the N-terminus. .. The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml).

    Article Title: Identification of Ser/Thr kinase and Forkhead Associated Domains in Mycobacterium ulcerans: Characterization of Novel Association between Protein Kinase Q and MupFHA
    Article Snippet: All of the Gromacs MD simulations were run in the HPC-Supercomputer facility (CSIR-IGIB, India) on 32 Cores at 11.4 ns/day maximum performance speed. .. Escherichia coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) for the expression of recombinant proteins. .. E. coli cells were grown and maintained with constant shaking (200 rpm) at 37°C in LB broth supplemented with appropriate antibiotic (100 µg/ml ampicillin and/or 12.5 µg/ml chloramphenicol), when needed.

    Article Title: Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
    Article Snippet: pMD20-T (Takara, Otsu, Japan) was employed to clone gene and pET-21b (Novagen, Madison, USA) was employed to construct expression plasmid. .. E. coli strain DH5α (Novagen, Madison, USA) was employed for gene manipulation and BL21 (DE3; Novagen, Madison, USA) was employed to construct recombinant expression strain. .. Recombinant IFN-CSP/pET-21b expression plasmid was obtained from Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou, People’s Republic of China.

    Article Title: Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis
    Article Snippet: Future studies will be needed to define role of GroEL in biofilm formation and cell surface attachment in B. anthracis . .. E. coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) was used for the expression of recombinant proteins. .. E. coli cells were grown as described before., B. anthracis Sterne strain (wild-type Bas -wt and Bas ΔprkC ), and B. subtilis were grown in LB broth at 37 °C with shaking at 200 rpm.

    Article Title: Understanding the Role of PknJ in Mycobacterium tuberculosis: Biochemical Characterization and Identification of Novel Substrate Pyruvate Kinase A
    Article Snippet: Ser37 is the influential residue for mtPykA which aids in ATP and pyruvate generation and is also identified as one of the phosphorylation site. .. E. coli strain DH5α (Novagen) was used for cloning and BL21 (DE3) (Stratagene) for the expression of recombinant proteins. .. E. coli cells were grown and maintained with constant shaking (220 rpm) at 37°C in LB medium supplemented with 100 µg/ml ampicillin and/or 40 µg/ml kanamycin, when needed.

    Molecular Cloning:

    Article Title: Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana
    Article Snippet: Paragraph title: 2.1.1. Molecular cloning   ... Escherichia coli strain DH5α (Novagen) was used for plasmid cloning, and the insertion of the gene for the APR1 glutaredoxin domain was confirmed by DNA sequencing.

    Mutagenesis:

    Article Title: Genetic and Mass Spectrometry Analyses of the Unusual Type IV-Like Pili of the Archaeon Methanococcus maripaludis
    Article Snippet: For the in-frame deletion mutagenesis experiments with M. maripaludis Mm900, cells were grown in McCas medium supplemented with 8-azahypoxanthine (240 μg/ml) or neomycin (1 mg/ml) as required at various steps in the transformation procedure outlined by Moore and Leigh ( ). .. Escherichia coli strain DH5α (Novagen) was used for intermediate cloning steps.

    Subcloning:

    Article Title: Design, Construction and Cloning of Truncated ORF2 and tPAsp-PADRE-Truncated ORF2 Gene Cassette From Hepatitis E Virus in the pVAX1 Expression Vector
    Article Snippet: Furthermore, the main goal of the present study was to construct the recombinant plasmids pVAX-tPAsp -PADRE-truncated ORF2 and pVAX-truncated ORF2 as DNA vaccine candidates. .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pVAX1 plasmid (Invitrogen® , Carls-bad, CA, USA) was used for subcloning of the truncated ORF2 and tPAsp-PADRE-truncated ORF2 (112 - 660 aa) gene cassette. .. Eukaryotic expression vector, pVAX1, was constructed to be consistent with the Food and Drug Administration (FDA) regulations.

    Article Title: A strategy to identify a ketoreductase that preferentially synthesizes pharmaceutically relevant (S)-alcohols using whole-cell biotransformation
    Article Snippet: This understanding of the catalytic mechanism and cofactor augmentation can be extended to other ketoreductases and substrates to reduce the enzyme screening space and enable manufacture of high value chiral alcohols. .. Escherichia coli strains DH5α (Novagen, U SA) and BL21(DE3) (Novagen, USA) are used as hosts for sub-cloning and over-expression respectively. .. Restriction enzymes NdeI and XhoI are purchased from NEB (New England Biolabs).

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells
    Article Snippet: S. enterica subsp. enteric serovar typhimurium (ATCC 14028) was obtained from the American Type Culture Collection (ATCC, Manassas, VA., USA) and cultured on Salmonella Shigella agar medium (Oxoid CM0099, UK ) and Neutrient broth (Himedia, India) at 37ºC and 5% CO2 . .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pPrimecloning vector (5PRIME Inc. Germany) was used for cloning the PCR products, while pVAX1 (Invitrogen®, Carlsbad, CA, USA) was used for sub-cloning the fliC gene. .. Eukaryotic expression vector pVAX1 carries the human cytomegalovirus (CMV) immediate early promoter, the bovine growth hormone (BGH) polyadenylation signal for transcription termination, the kanamycin resistance gene and the pUC origin of replication for maintenance in E. coli .

    Positron Emission Tomography:

    Article Title: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo
    Article Snippet: pMD20-T (Takara, Japan) was used for gene cloning. pET-21b (Novagen, USA) was employed to construct expression vector. .. E. coli strain DH5α (Novagen, USA) was applied as the host for gene manipulation.

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: The PCR product was cloned into the expression vector pET-32a [modified by inserting a Tobacco etch virus (TEV) protease cleavage site inside the NcoI site] with BamHI and XhoI. .. Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing.

    Article Title: Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
    Article Snippet: pMD20-T (Takara, Otsu, Japan) was employed to clone gene and pET-21b (Novagen, Madison, USA) was employed to construct expression plasmid. .. E. coli strain DH5α (Novagen, Madison, USA) was employed for gene manipulation and BL21 (DE3; Novagen, Madison, USA) was employed to construct recombinant expression strain.

    Article Title: Construction of a Novel Liver-Targeting Fusion Interferon by Incorporation of a Plasmodium Region I-Plus Peptide
    Article Snippet: We further investigated whether the fusion protein IFN-CSP reserves anti-HBV activity and liver-targeting function as its parental peptides. .. E. coli strain DH5α (Novagen, USA) and BL21 (DE3; Novagen, USA) were used as the host for gene manipulation and expression of fusion protein, respectively. pMD20-T (Takara, Japan) and pET-21b (Novagen, USA) were applied for gene cloning and expression, respectively. .. Luria-Bertani (LB) medium was employed for bacterial growth and protein production.

    cDNA Library Assay:

    Article Title: Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana
    Article Snippet: The DNA encoding amino acids 353–461 of A. thaliana APR1 (465 amino acids, TAIR accession No. At4G04610.1) was amplified by PCR using a prepared cDNA library and the gene-specific forward and reverse primers listed in Table 1 . .. Escherichia coli strain DH5α (Novagen) was used for plasmid cloning, and the insertion of the gene for the APR1 glutaredoxin domain was confirmed by DNA sequencing.

    SDS Page:

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml). .. The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml).

    Plasmid Preparation:

    Article Title: Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases
    Article Snippet: Paragraph title: Plasmid construction and recombinant yeast ... The resulting plasmids (p11α-SHAoch and pCYP509C12, respectively) were transformed into E. coli strain DH5α (Novagen) that were selected and maintained at 37 °C using Luria–Bertani medium supplemented with sodium ampicillin (100 mg/L).

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: The correct DNA insert (757 bp) was confirmed by digesting the plasmid with restriction enzymes XmnI and NotI. .. The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml). .. Fusion protein was isolated by affinity purification using the Nickel-charged 6 X histidine binding resin beads (Novagen).

    Article Title: Design, Construction and Cloning of Truncated ORF2 and tPAsp-PADRE-Truncated ORF2 Gene Cassette From Hepatitis E Virus in the pVAX1 Expression Vector
    Article Snippet: Furthermore, the main goal of the present study was to construct the recombinant plasmids pVAX-tPAsp -PADRE-truncated ORF2 and pVAX-truncated ORF2 as DNA vaccine candidates. .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pVAX1 plasmid (Invitrogen® , Carls-bad, CA, USA) was used for subcloning of the truncated ORF2 and tPAsp-PADRE-truncated ORF2 (112 - 660 aa) gene cassette. .. Eukaryotic expression vector, pVAX1, was constructed to be consistent with the Food and Drug Administration (FDA) regulations.

    Article Title: High-level expression of a novel liver-targeting fusion interferon with preferred Escherichia coli codon preference and its anti-hepatitis B virus activity in vivo
    Article Snippet: pMD20-T (Takara, Japan) was used for gene cloning. pET-21b (Novagen, USA) was employed to construct expression vector. .. E. coli strain DH5α (Novagen, USA) was applied as the host for gene manipulation.

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: The PCR product was cloned into the expression vector pET-32a [modified by inserting a Tobacco etch virus (TEV) protease cleavage site inside the NcoI site] with BamHI and XhoI. .. Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing. .. The recombinant plasmid was then transformed into E. coli strain BL21 (DE3) (Novagen) for protein expression.

    Article Title: Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution
    Article Snippet: Based in the recently solved crystal structure of the homodimeric wild type enzyme we rationalize the molecular basis behind the increased kinetic or thermodynamic stability of PpAzoR enzyme. .. Escherichia coli strain DH5α (Novagen) was used for routine propagation and amplification of plasmid constructs. .. E. coli Tuner (DE3, Novagen) and KRX (Promega) strains were used to express the ppAzoR and variant genes cloned in pET-21a (+) plasmid (Novagen).

    Article Title: Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana
    Article Snippet: The PCR product was cloned into the plasmid pGEX-4T1 (GE Healthcare) that encodes an upstream glutathione S -transferase (GST) tag followed by a thrombin cleavage site. .. Escherichia coli strain DH5α (Novagen) was used for plasmid cloning, and the insertion of the gene for the APR1 glutaredoxin domain was confirmed by DNA sequencing. .. The recombinant plasmid was transformed into E. coli strain BL21(DE3) (Novagen) for protein expression.

    Article Title: Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
    Article Snippet: pMD20-T (Takara, Otsu, Japan) was employed to clone gene and pET-21b (Novagen, Madison, USA) was employed to construct expression plasmid. .. E. coli strain DH5α (Novagen, Madison, USA) was employed for gene manipulation and BL21 (DE3; Novagen, Madison, USA) was employed to construct recombinant expression strain.

    Article Title: W8, a new Sup35 prion strain, transmits distinctive information with a conserved assembly scheme
    Article Snippet: Standard protocols were used for media preparation and yeast genetic manipulation. .. Escherichia coli strains DH5α and BLR(DE3)pLysS (Novagen) were used for plasmid construction and protein expression, respectively. .. White, pink and dark pink colonies were selected for strain typing.

    Article Title: Modification of the Sweetness and Stability of Sweet-Tasting Protein Monellin by Gene Mutation and Protein Engineering
    Article Snippet: The results proved that the thermal stability and sweetness of the natural monellin protein can be improved by gene mutation technique, which could realize the industrial production of monellin and provide a new kind of sugar substitute for human being. .. Escherichia coli strains DH5α and BL21-CodonPlus(DE3)-RIL and plasmid pET15b were from Novagen. .. Easy Pfu DNA polymerase and restriction enzyme FastDigest DpnI were from Beijing TransGen Biotech Co. and Thermo Scientific, respectively.

    Article Title: SCO5745, a Bifunctional RNase J Ortholog, Affects Antibiotic Production in Streptomyces coelicolor
    Article Snippet: We also demonstrate that sco5745 is not essential and that disruption of the gene affects antibiotic production in the disrupted strain. .. Escherichia coli strains DH5α and Rosetta 2(DE3)pLysS (Novagen) were used as hosts for plasmid manipulation and protein overexpression, respectively. .. E. coli strains BW25113 and ET12567 ( dam dcm hsdS ) were used for gene disruption and conjugation purposes ( ).

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells
    Article Snippet: S. enterica subsp. enteric serovar typhimurium (ATCC 14028) was obtained from the American Type Culture Collection (ATCC, Manassas, VA., USA) and cultured on Salmonella Shigella agar medium (Oxoid CM0099, UK ) and Neutrient broth (Himedia, India) at 37ºC and 5% CO2 . .. Escherichia coli strain DH5α (Novagen Inc., Madison, Wis., USA) was used for transformation and the pPrimecloning vector (5PRIME Inc. Germany) was used for cloning the PCR products, while pVAX1 (Invitrogen®, Carlsbad, CA, USA) was used for sub-cloning the fliC gene. .. Eukaryotic expression vector pVAX1 carries the human cytomegalovirus (CMV) immediate early promoter, the bovine growth hormone (BGH) polyadenylation signal for transcription termination, the kanamycin resistance gene and the pUC origin of replication for maintenance in E. coli .

    Selection:

    Article Title: Genetic and Mass Spectrometry Analyses of the Unusual Type IV-Like Pili of the Archaeon Methanococcus maripaludis
    Article Snippet: In complementation experiments, the plasmids were maintained in M. maripaludis by puromycin (2.5 μg/ml) selection. .. Escherichia coli strain DH5α (Novagen) was used for intermediate cloning steps.

    Concentration Assay:

    Article Title: The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
    Article Snippet: Escherichia coli strain DH5α (Novagen) was used for plasmid amplification, which was confirmed by DNA sequencing. .. The recombinant plasmid was then transformed into E. coli strain BL21 (DE3) (Novagen) for protein expression.

    Staining:

    Article Title: The haptoglobin beta subunit sequesters HMGB1 toxicity in sterile and infectious inflammation
    Article Snippet: The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml). .. The plasmid was transformed into E coli strain DH5α (Novagen, Madison, WI) and incubated with 2-YT medium containing carbenicillin (100 μg/ml).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89
    Millipore escherichia coli strains dh5α
    Escherichia Coli Strains Dh5α, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strains dh5α/product/Millipore
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strains dh5α - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    Image Search Results