escherichia coli strain novablue  (Millipore)


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    Escherichia coli
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    Catalog Number:
    ec9637
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    Structured Review

    Millipore escherichia coli strain novablue

    https://www.bioz.com/result/escherichia coli strain novablue/product/Millipore
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain novablue - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Relationships between Cargo, Cell Penetrating Peptidesand Cell Type for Uptake of Non-Covalent Complexes into Live Cells
    Article Snippet: .. Cargoes and Reagents: Fluorescent-labeled nucleotide: Aminoallyl ATTO488-dUTP, (Jena Bioscience), fluorescent-labeled antibodies: polyclonal, goat anti mouse, FITC-labeled (Jena Bioscience), fluorescence-labeled BSA (ATTO488-BSA, Jena Bioscience), β-galactosidase (E. coli , 540 kDa, Calbiochem, Darmstadt, Germany), X-gal (99 %, Calbiochem), trypsin/EDTA solution (trypsin/EDTA concentrate, PAA, Pasching, Austria), DAPI (Sigma-Aldrich Chemie), 1 % penicillin/streptomycin and Hemin (Jena Bioscience). .. Kits : Cell viability assay: MTT assay (Jena Bioscience), membrane integrity assay: bioluminescence test CytoTox-Glo (Promega, Madison, WI, USA), Mycoplasma test (Mycoplasma Detection Kit, Jena Bioscience).

    Cell Culture:

    Article Title: Control of siRNA expression using the Cre-loxP recombination system
    Article Snippet: .. For residual E.coli lysate, pTriEx-3 Hygro empty vector was introduced into E.coli strain RosettaBlue(DE3)pLacI (Novagen) and cultured in LB medium that contained 1% glucose, 34 µg/ml chloramphenicol, 12.5 µg/ml tetracycline and 100 µg/ml ampicillin at 37°C until absorbance at 600 nm reached 0.7. .. After addition of IPTG to a final concentration of 1 mM, E.coli were harvested and treated in the same way as that of pTriEx-3 Hygro-TAT-NLS-Cre or pTriEx-3 Hygro-TAT-NLS-EGFP.

    Mouse Assay:

    Article Title: Repetitive Injections of Dendritic Cells Matured with Tumor Necrosis Factor ? Induce Antigen-specific Protection of Mice from Autoimmunity
    Article Snippet: .. For DC maturation, cultures were pulsed for 4 h with TNF-α (500 U/ml; TEBU/PeproTech) or with LPS (1 μg/ml; Sigma-Aldrich; E. coli , 0127:B8) with or without anti-CD40 (clone 3/23 or HM40, 5 μg/ml; BD PharMingen) together with or without 10 μM MOG peptide (see below) before intravenous injection into the tail vein of mice. .. To generate immature DCs, BM cells were cultured continuously in the presence of 20 ng/ml IL-10 (BD PharMingen; reference ).

    Acid Assay:

    Article Title: Synthesis and evaluation of the anti-inflammatory properties of selenium-derivatives of celecoxib
    Article Snippet: .. Expression studies were carried out in RAW264.7 macrophages treated with DMSO, celecoxib, selenocoxib-2, selenocoxib-3 (0.1 and 1.0 μM) for 12 h followed by E. coli LPS (1 μg/ml; Serotype: 0111:B4; Sigma-Aldrich) stimulation for 2–8 h. Protein estimation in the lysates was performed using the biocinchoninic acid assay (Thermo Pierce) and equal amounts of protein were used for SDS-PAGE and Western blotting with antibodies specific to GPX1, COX-2, or iNOS. ..

    Incubation:

    Article Title: Evaluation of Wi-Fi Radiation Effects on Antibiotic Susceptibility, Metabolic Activity and Biofilm Formation by Escherichia Coli 0157H7,Staphylococcus Aureus and Staphylococcus Epidermis
    Article Snippet: .. Therefore, a single colony of E. coli 0157H7 was inoculated in 5 ml of Luria-Bertani (LB) broth (Sigma-Aldrich) and incubated at 37°C overnight with agitation at 200 rpm. .. Bacterial overnight culture (1μl) was injected into three spots on freshly prepared motility agar plates containing 0.5% agar (Motility Test Medium Himedia) [ ].

    Quantitative RT-PCR:

    Article Title: Intestinal Epithelial Apoptosis initiates Gut Mucosal Injury during Extracorporeal Membrane Oxygenation in the Newborn Piglet
    Article Snippet: .. In some experiments, we treated mast cells with E. coli LPS (0.1–1 μg/mL) and/or the p38 inhibitor SB202190 (Sigma) overnight and measured fas ligand expression by RT-qPCR. .. Statistical methods Parametric and non-parametric tests were applied using the Sigma Stat 3.1.1 software (Systat, Point Richmond, CA).

    Expressing:

    Article Title: Intestinal Epithelial Apoptosis initiates Gut Mucosal Injury during Extracorporeal Membrane Oxygenation in the Newborn Piglet
    Article Snippet: .. In some experiments, we treated mast cells with E. coli LPS (0.1–1 μg/mL) and/or the p38 inhibitor SB202190 (Sigma) overnight and measured fas ligand expression by RT-qPCR. .. Statistical methods Parametric and non-parametric tests were applied using the Sigma Stat 3.1.1 software (Systat, Point Richmond, CA).

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: .. For construction of E.coli expression plasmid pKN203 a Nde I– Bam HI full-length hDBR1 cDNA fragment from pKN201 was subcloned into Nde I and Bam HI digested pET28a (Novagen). .. The yeast expression plasmids pKN205 and pKN206 were constructed by inserting an Eco RI– Bam HI full-length hDBR1 cDNA fragment from pKN201 into S.cerevisiae expression vectors pRS314GU ( ) and pRS316GU ( ) digested with Eco RI and Bam HI, respectively.

    Article Title: Synthesis and evaluation of the anti-inflammatory properties of selenium-derivatives of celecoxib
    Article Snippet: .. Expression studies were carried out in RAW264.7 macrophages treated with DMSO, celecoxib, selenocoxib-2, selenocoxib-3 (0.1 and 1.0 μM) for 12 h followed by E. coli LPS (1 μg/ml; Serotype: 0111:B4; Sigma-Aldrich) stimulation for 2–8 h. Protein estimation in the lysates was performed using the biocinchoninic acid assay (Thermo Pierce) and equal amounts of protein were used for SDS-PAGE and Western blotting with antibodies specific to GPX1, COX-2, or iNOS. ..

    Lysis:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Western Blot:

    Article Title: Synthesis and evaluation of the anti-inflammatory properties of selenium-derivatives of celecoxib
    Article Snippet: .. Expression studies were carried out in RAW264.7 macrophages treated with DMSO, celecoxib, selenocoxib-2, selenocoxib-3 (0.1 and 1.0 μM) for 12 h followed by E. coli LPS (1 μg/ml; Serotype: 0111:B4; Sigma-Aldrich) stimulation for 2–8 h. Protein estimation in the lysates was performed using the biocinchoninic acid assay (Thermo Pierce) and equal amounts of protein were used for SDS-PAGE and Western blotting with antibodies specific to GPX1, COX-2, or iNOS. ..

    Injection:

    Article Title: Repetitive Injections of Dendritic Cells Matured with Tumor Necrosis Factor ? Induce Antigen-specific Protection of Mice from Autoimmunity
    Article Snippet: .. For DC maturation, cultures were pulsed for 4 h with TNF-α (500 U/ml; TEBU/PeproTech) or with LPS (1 μg/ml; Sigma-Aldrich; E. coli , 0127:B8) with or without anti-CD40 (clone 3/23 or HM40, 5 μg/ml; BD PharMingen) together with or without 10 μM MOG peptide (see below) before intravenous injection into the tail vein of mice. .. To generate immature DCs, BM cells were cultured continuously in the presence of 20 ng/ml IL-10 (BD PharMingen; reference ).

    Purification:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    SDS Page:

    Article Title: Synthesis and evaluation of the anti-inflammatory properties of selenium-derivatives of celecoxib
    Article Snippet: .. Expression studies were carried out in RAW264.7 macrophages treated with DMSO, celecoxib, selenocoxib-2, selenocoxib-3 (0.1 and 1.0 μM) for 12 h followed by E. coli LPS (1 μg/ml; Serotype: 0111:B4; Sigma-Aldrich) stimulation for 2–8 h. Protein estimation in the lysates was performed using the biocinchoninic acid assay (Thermo Pierce) and equal amounts of protein were used for SDS-PAGE and Western blotting with antibodies specific to GPX1, COX-2, or iNOS. ..

    Plasmid Preparation:

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: .. For construction of E.coli expression plasmid pKN203 a Nde I– Bam HI full-length hDBR1 cDNA fragment from pKN201 was subcloned into Nde I and Bam HI digested pET28a (Novagen). .. The yeast expression plasmids pKN205 and pKN206 were constructed by inserting an Eco RI– Bam HI full-length hDBR1 cDNA fragment from pKN201 into S.cerevisiae expression vectors pRS314GU ( ) and pRS316GU ( ) digested with Eco RI and Bam HI, respectively.

    Article Title: Control of siRNA expression using the Cre-loxP recombination system
    Article Snippet: .. For residual E.coli lysate, pTriEx-3 Hygro empty vector was introduced into E.coli strain RosettaBlue(DE3)pLacI (Novagen) and cultured in LB medium that contained 1% glucose, 34 µg/ml chloramphenicol, 12.5 µg/ml tetracycline and 100 µg/ml ampicillin at 37°C until absorbance at 600 nm reached 0.7. .. After addition of IPTG to a final concentration of 1 mM, E.coli were harvested and treated in the same way as that of pTriEx-3 Hygro-TAT-NLS-Cre or pTriEx-3 Hygro-TAT-NLS-EGFP.

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  • 99
    Millipore escherichia coli strain novablue
    Escherichia Coli Strain Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain novablue/product/Millipore
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain novablue - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    85
    Millipore competent e coli novablue cells
    Competent E Coli Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli novablue cells/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    competent e coli novablue cells - by Bioz Stars, 2020-05
    85/100 stars
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    85
    Millipore novablue e coli amplification strain
    Novablue E Coli Amplification Strain, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue e coli amplification strain/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    novablue e coli amplification strain - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

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