escherichia coli strain novablue gigasingles competent cells  (Millipore)


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    Structured Review

    Millipore escherichia coli strain novablue gigasingles competent cells
    Escherichia Coli Strain Novablue Gigasingles Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain novablue gigasingles competent cells/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain novablue gigasingles competent cells - by Bioz Stars, 2020-04
    92/100 stars

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    Clone Assay:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: Paragraph title: Cloning and generation of recombinant AKAP18 β and the D/D domain of RII α ... The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen).

    Amplification:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: .. The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen). .. The untagged RII α -D/D domain (amino acids 1–44) was cloned into pET46 by removing the N-terminal His-tag from His-D/D-RII α in pET46 Ek/LIC [ ] by PCR with the primer pair DD forward: 5′-GGTCA GCCAT GGCAA GCCAC ATCCA GAT-3′, reverse: 5′-CGTCT TCTCG AGTTA GCGGG CCTCG CG-3′, and restriction with NcoI/XhoI.

    Ligation:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: An AKAP18 β cDNA fragment encoding amino acid residues 43–83 (NGGEP DDAEL VRLSK RLVEN AVLKA VQQY LEETQ NKNKP GE) was cloned by ligation-independent cloning (LIC) into vector pET30 Ek/LIC (Merck Millipore). .. The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen).

    Purification:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: .. The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen). .. The untagged RII α -D/D domain (amino acids 1–44) was cloned into pET46 by removing the N-terminal His-tag from His-D/D-RII α in pET46 Ek/LIC [ ] by PCR with the primer pair DD forward: 5′-GGTCA GCCAT GGCAA GCCAC ATCCA GAT-3′, reverse: 5′-CGTCT TCTCG AGTTA GCGGG CCTCG CG-3′, and restriction with NcoI/XhoI.

    Polymerase Chain Reaction:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: PCR was used for introduction of an additional PreScission recognition site for His-tag removal. .. The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen).

    Transformation Assay:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: .. The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen). .. The untagged RII α -D/D domain (amino acids 1–44) was cloned into pET46 by removing the N-terminal His-tag from His-D/D-RII α in pET46 Ek/LIC [ ] by PCR with the primer pair DD forward: 5′-GGTCA GCCAT GGCAA GCCAC ATCCA GAT-3′, reverse: 5′-CGTCT TCTCG AGTTA GCGGG CCTCG CG-3′, and restriction with NcoI/XhoI.

    Recombinant:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: Paragraph title: Cloning and generation of recombinant AKAP18 β and the D/D domain of RII α ... The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen).

    Crystallization Assay:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen). .. For crystallization, the two proteins were co-expressed in E. coli strain Rosetta 2 DE3 in the presence of the antibiotics kanamycin, ampicillin and chloramphenicol.

    Plasmid Preparation:

    Article Title: AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA
    Article Snippet: .. The AKAP18 β (43–83)-encoding cDNA fragment was amplified from a pCMV6-XL5 clone (Origene, ) and purified, and 0.2 pmol was treated with T4 DNA polymerase and annealed with the vector pET30 Ek/LIC, which was transformed into Escherichia coli strain NovaBlue GigaSingles-competent cells (Novagen). .. The untagged RII α -D/D domain (amino acids 1–44) was cloned into pET46 by removing the N-terminal His-tag from His-D/D-RII α in pET46 Ek/LIC [ ] by PCR with the primer pair DD forward: 5′-GGTCA GCCAT GGCAA GCCAC ATCCA GAT-3′, reverse: 5′-CGTCT TCTCG AGTTA GCGGG CCTCG CG-3′, and restriction with NcoI/XhoI.

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    Millipore e coli strain novablue gigasingles
    E Coli Strain Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain novablue gigasingles/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli strain novablue gigasingles - by Bioz Stars, 2020-04
    86/100 stars
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