escherichia coli strain novablue de3  (Millipore)


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    Structured Review

    Millipore escherichia coli strain novablue de3
    Escherichia Coli Strain Novablue De3, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain novablue de3/product/Millipore
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain novablue de3 - by Bioz Stars, 2020-04
    81/100 stars

    Related Products / Commonly Used Together

    construct hexahistidine-pmp22

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    Related Articles

    In Vivo:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: Deubiquitylating enzyme activity assays The ability to release Ub linked via α-amino linkages was assayed in vivo using the substrates: ubiquitin-extension protein At UBQ1 (p8185) and the hexameric polyubiquitin At UBQ10 , ; the latter had the transcription start site modified to attenuate expression (p AtUBQ10-LE as described in Yan et al . .. The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a.

    In Vitro:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a. .. The cleavage of Ubs connected via ε-amino isopeptide bonds used lysine-48-linked poly-Ub chains synthesized in vitro by the wheat E2 UBC7 as described .

    Positive Control:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a. .. Similarly expressed UCH2 was included as a positive control.

    Synthesized:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a. .. The cleavage of Ubs connected via ε-amino isopeptide bonds used lysine-48-linked poly-Ub chains synthesized in vitro by the wheat E2 UBC7 as described .

    Mutagenesis:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: .. The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a. ..

    Construct:

    Article Title: Arabidopsis 22-Kilodalton Peroxisomal Membrane Protein. Nucleotide Sequence Analysis and Biochemical Characterization 1
    Article Snippet: .. The construct hexahistidine-PMP22:pET16b was transformed into Escherichia coli strain NovaBlue DE3 (Novagen, Madison, WI). .. Recombinant hexahistidine-PMP22 production in exponentially growing cells was induced by 500 μ m isopropylthio-β-galactoside over 4 h at 37°C, and the protein was purified by its affinity to nickel-agarose (Qiagen, Chatsworth, CA) under denaturing conditions according to the manufacturer's recommendations.

    Purification:

    Article Title: Arabidopsis 22-Kilodalton Peroxisomal Membrane Protein. Nucleotide Sequence Analysis and Biochemical Characterization 1
    Article Snippet: Paragraph title: Recombinant Hexahistidine-PMP22 Production and Purification ... The construct hexahistidine-PMP22:pET16b was transformed into Escherichia coli strain NovaBlue DE3 (Novagen, Madison, WI).

    Activity Assay:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: Paragraph title: Deubiquitylating enzyme activity assays ... The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a.

    Expressing:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: Deubiquitylating enzyme activity assays The ability to release Ub linked via α-amino linkages was assayed in vivo using the substrates: ubiquitin-extension protein At UBQ1 (p8185) and the hexameric polyubiquitin At UBQ10 , ; the latter had the transcription start site modified to attenuate expression (p AtUBQ10-LE as described in Yan et al . .. The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a.

    Modification:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: Deubiquitylating enzyme activity assays The ability to release Ub linked via α-amino linkages was assayed in vivo using the substrates: ubiquitin-extension protein At UBQ1 (p8185) and the hexameric polyubiquitin At UBQ10 , ; the latter had the transcription start site modified to attenuate expression (p AtUBQ10-LE as described in Yan et al . .. The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a.

    Transformation Assay:

    Article Title: Arabidopsis 22-Kilodalton Peroxisomal Membrane Protein. Nucleotide Sequence Analysis and Biochemical Characterization 1
    Article Snippet: .. The construct hexahistidine-PMP22:pET16b was transformed into Escherichia coli strain NovaBlue DE3 (Novagen, Madison, WI). .. Recombinant hexahistidine-PMP22 production in exponentially growing cells was induced by 500 μ m isopropylthio-β-galactoside over 4 h at 37°C, and the protein was purified by its affinity to nickel-agarose (Qiagen, Chatsworth, CA) under denaturing conditions according to the manufacturer's recommendations.

    Recombinant:

    Article Title: Arabidopsis 22-Kilodalton Peroxisomal Membrane Protein. Nucleotide Sequence Analysis and Biochemical Characterization 1
    Article Snippet: Paragraph title: Recombinant Hexahistidine-PMP22 Production and Purification ... The construct hexahistidine-PMP22:pET16b was transformed into Escherichia coli strain NovaBlue DE3 (Novagen, Madison, WI).

    Plasmid Preparation:

    Article Title: Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
    Article Snippet: .. The coding regions for each substrate was introduced into a pACYC184-based plasmid and was co-expressed in the E. coli strain NovaBlue (DE3) (Novagen) with wild-type UCH3 or its C101A or C101S mutant version present in pET32a. ..

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  • 89
    Millipore escherichia coli de3 novablue cells
    Escherichia Coli De3 Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli de3 novablue cells/product/Millipore
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli de3 novablue cells - by Bioz Stars, 2020-04
    89/100 stars
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