escherichia coli strain bl21 rosetta 2  (Millipore)


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    Escherichia coli
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    ec9637
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    Structured Review

    Millipore escherichia coli strain bl21 rosetta 2

    https://www.bioz.com/result/escherichia coli strain bl21 rosetta 2/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain bl21 rosetta 2 - by Bioz Stars, 2020-05
    99/100 stars

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    Cell Culture:

    Article Title: Control of siRNA expression using the Cre-loxP recombination system
    Article Snippet: .. For residual E.coli lysate, pTriEx-3 Hygro empty vector was introduced into E.coli strain RosettaBlue(DE3)pLacI (Novagen) and cultured in LB medium that contained 1% glucose, 34 µg/ml chloramphenicol, 12.5 µg/ml tetracycline and 100 µg/ml ampicillin at 37°C until absorbance at 600 nm reached 0.7. .. After addition of IPTG to a final concentration of 1 mM, E.coli were harvested and treated in the same way as that of pTriEx-3 Hygro-TAT-NLS-Cre or pTriEx-3 Hygro-TAT-NLS-EGFP.

    Purification:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Incubation:

    Article Title: Extracellular Vesicles from Leishmania-Infected Macrophages Confer an Anti-infection Cytokine-Production Profile to Naïve Macrophages
    Article Snippet: .. These peritoneal macrophages were incubated for 48 hours with EVs from uninfected bone marrow-derived macrophages (EVmedium) and with EVs from L. amazonensis -infected bone marrow-derived macrophages (EVLa) with or without 1 ng/mL bacterial lipopolysacharide (LPS; Escherichia coli 0127:B8, Sigma-Aldridge, St. Louis, MO, USA), at 37°C and 5% CO2 . .. In cultures that received EVmedium and EVLa in the absence of LPS, 10 µg/mL of polymyxin B (Sigma-Aldridge, St. Louis, CA, USA) were added to rule out the possibility that a putative LPS contamination could compromise the interpretation of results.

    Article Title: Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer
    Article Snippet: .. E. coli ME8077 was transformed with pTSMb1 and incubated in 40 ml of LB liquid medium containing 100 μg/ml of kanamycin and 20 ng/ml of mitomycin C at 37°C for 12 h. Cultures were filtered with membrane filters (Millex-SV [Millipore]; pore size, 5.0 μm). .. The filtrate was also refiltered twice with membrane filters (Millex-AA [Millipore]; pore size, 0.8 μm) to remove normal cells.

    Infection:

    Article Title: Extracellular Vesicles from Leishmania-Infected Macrophages Confer an Anti-infection Cytokine-Production Profile to Naïve Macrophages
    Article Snippet: .. These peritoneal macrophages were incubated for 48 hours with EVs from uninfected bone marrow-derived macrophages (EVmedium) and with EVs from L. amazonensis -infected bone marrow-derived macrophages (EVLa) with or without 1 ng/mL bacterial lipopolysacharide (LPS; Escherichia coli 0127:B8, Sigma-Aldridge, St. Louis, MO, USA), at 37°C and 5% CO2 . .. In cultures that received EVmedium and EVLa in the absence of LPS, 10 µg/mL of polymyxin B (Sigma-Aldridge, St. Louis, CA, USA) were added to rule out the possibility that a putative LPS contamination could compromise the interpretation of results.

    Expressing:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: .. For construction of E.coli expression plasmid pKN203 a Nde I– Bam HI full-length hDBR1 cDNA fragment from pKN201 was subcloned into Nde I and Bam HI digested pET28a (Novagen). .. The yeast expression plasmids pKN205 and pKN206 were constructed by inserting an Eco RI– Bam HI full-length hDBR1 cDNA fragment from pKN201 into S.cerevisiae expression vectors pRS314GU ( ) and pRS316GU ( ) digested with Eco RI and Bam HI, respectively.

    Article Title: Screen of Non-annotated Small Secreted Proteins of Pseudomonas syringae Reveals a Virulence Factor That Inhibits Tomato Immune Proteases
    Article Snippet: .. Efficient protein production using an adjusted bacterial expression system for secreted proteins To produce the selected proteins heterologously, we took advantage of the commercially available pFLAG-ATS expression system in Escherichia coli (Sigma-Aldrich). .. This expression system secretes N-terminally FLAG-tagged proteins into the growth medium using an N-terminal OmpA signal peptide to facilitate secretion.

    Lysis:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Transformation Assay:

    Article Title: Regeneration of Escherichia coli from Minicells through Lateral Gene Transfer
    Article Snippet: .. E. coli ME8077 was transformed with pTSMb1 and incubated in 40 ml of LB liquid medium containing 100 μg/ml of kanamycin and 20 ng/ml of mitomycin C at 37°C for 12 h. Cultures were filtered with membrane filters (Millex-SV [Millipore]; pore size, 5.0 μm). .. The filtrate was also refiltered twice with membrane filters (Millex-AA [Millipore]; pore size, 0.8 μm) to remove normal cells.

    Plasmid Preparation:

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: .. For construction of E.coli expression plasmid pKN203 a Nde I– Bam HI full-length hDBR1 cDNA fragment from pKN201 was subcloned into Nde I and Bam HI digested pET28a (Novagen). .. The yeast expression plasmids pKN205 and pKN206 were constructed by inserting an Eco RI– Bam HI full-length hDBR1 cDNA fragment from pKN201 into S.cerevisiae expression vectors pRS314GU ( ) and pRS316GU ( ) digested with Eco RI and Bam HI, respectively.

    Article Title: Control of siRNA expression using the Cre-loxP recombination system
    Article Snippet: .. For residual E.coli lysate, pTriEx-3 Hygro empty vector was introduced into E.coli strain RosettaBlue(DE3)pLacI (Novagen) and cultured in LB medium that contained 1% glucose, 34 µg/ml chloramphenicol, 12.5 µg/ml tetracycline and 100 µg/ml ampicillin at 37°C until absorbance at 600 nm reached 0.7. .. After addition of IPTG to a final concentration of 1 mM, E.coli were harvested and treated in the same way as that of pTriEx-3 Hygro-TAT-NLS-Cre or pTriEx-3 Hygro-TAT-NLS-EGFP.

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  • 99
    Millipore escherichia coli strain bl21 rosetta 2
    Escherichia Coli Strain Bl21 Rosetta 2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain bl21 rosetta 2/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain bl21 rosetta 2 - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Millipore e coli strain bl21 rosetta 2 de3
    E Coli Strain Bl21 Rosetta 2 De3, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain bl21 rosetta 2 de3/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli strain bl21 rosetta 2 de3 - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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