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TaKaRa escherichia coli stellar cells
Escherichia Coli Stellar Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/escherichia coli stellar cells/product/TaKaRa
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
escherichia coli stellar cells - by Bioz Stars, 2020-08
88/100 stars

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pjc45 vector
s. ratti
t. suis
eurofins mwg
trx-lp pcr fragments

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Related Articles

Clone Assay:

Article Title: Coassociation between Group B Streptococcus and Candida albicans Promotes Interactions with Vaginal Epithelium
Article Snippet: .. The resultant amplicon was cloned into vector pHY304 ( ) via XbaI and BamHI sites and propagated in E. coli Stellar cells (Clontech) prior to isolation and electroporation into GBS 515. .. An L. lactis strain expressing BspA had been generated previously , and a similar methodology was employed here to generate an L. lactis strain expressing BspC.

Article Title: Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis
Article Snippet: .. The Trx-lp PCR fragments from S. ratti and T. suis were cloned into pJC45 vector [ ] and IBA 3 plus vector, transformed into Escherichia coli Stellar cells (Clontech, USA) and sequenced (eurofins MWG). ..

Article Title: Non-lethal inhibition of gut microbial trimethylamine production for the treatment of atherosclerosis
Article Snippet: .. Sequence verified clones were transformed into E. coli Stellar cells (Clontech, Moutainview, CA). .. The ORFs for Desulfovibrio alaskensis G20 cutC/D genes (Ddes_3282 and Ddes_1357, respectively) and their associated intergenic region were codon optimized for expression in E. coli and synthesized by Genscript for cloning into pUC57.

Amplification:

Article Title: Coassociation between Group B Streptococcus and Candida albicans Promotes Interactions with Vaginal Epithelium
Article Snippet: .. The resultant amplicon was cloned into vector pHY304 ( ) via XbaI and BamHI sites and propagated in E. coli Stellar cells (Clontech) prior to isolation and electroporation into GBS 515. .. An L. lactis strain expressing BspA had been generated previously , and a similar methodology was employed here to generate an L. lactis strain expressing BspC.

Electroporation:

Article Title: Coassociation between Group B Streptococcus and Candida albicans Promotes Interactions with Vaginal Epithelium
Article Snippet: .. The resultant amplicon was cloned into vector pHY304 ( ) via XbaI and BamHI sites and propagated in E. coli Stellar cells (Clontech) prior to isolation and electroporation into GBS 515. .. An L. lactis strain expressing BspA had been generated previously , and a similar methodology was employed here to generate an L. lactis strain expressing BspC.

Isolation:

Article Title: Coassociation between Group B Streptococcus and Candida albicans Promotes Interactions with Vaginal Epithelium
Article Snippet: .. The resultant amplicon was cloned into vector pHY304 ( ) via XbaI and BamHI sites and propagated in E. coli Stellar cells (Clontech) prior to isolation and electroporation into GBS 515. .. An L. lactis strain expressing BspA had been generated previously , and a similar methodology was employed here to generate an L. lactis strain expressing BspC.

Cell Culture:

Article Title: Characterization of Engineered PreQ1 Riboswitches for Inducible Gene Regulation in Mycobacteria
Article Snippet: .. E. coli Stellar cells (Clontech) were cultured on LB agar or liquid medium with 50 μg/ml kanamycin. .. M. smegmatis was incubated at 37°C and, for liquid cultures, at 225 rpm.

Polymerase Chain Reaction:

Article Title: Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis
Article Snippet: .. The Trx-lp PCR fragments from S. ratti and T. suis were cloned into pJC45 vector [ ] and IBA 3 plus vector, transformed into Escherichia coli Stellar cells (Clontech, USA) and sequenced (eurofins MWG). ..

Selection:

Article Title: Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae
Article Snippet: .. Initially, florfenicol resistance was transferred to E. coli Stellar cells (Clontech) by heat shock with the MIDG3446 plasmid extract, according to the manufacturer's protocol, with selection of transformants on LB agar supplemented with 10 μg/ml florfenicol. ..

Sequencing:

Article Title: Non-lethal inhibition of gut microbial trimethylamine production for the treatment of atherosclerosis
Article Snippet: .. Sequence verified clones were transformed into E. coli Stellar cells (Clontech, Moutainview, CA). .. The ORFs for Desulfovibrio alaskensis G20 cutC/D genes (Ddes_3282 and Ddes_1357, respectively) and their associated intergenic region were codon optimized for expression in E. coli and synthesized by Genscript for cloning into pUC57.

Conjugation Assay:

Article Title: Fatty Acid Biosynthesis in Pseudomonas aeruginosa Is Initiated by the FabY Class of ?-Ketoacyl Acyl Carrier Protein Synthases
Article Snippet: .. The vectors were mobilized in trans using either E. coli SM10 ( ) or E. coli Stellar cells (Clontech) transformed with the helper plasmid pRK2013 ( ) as the conjugation-proficient donor strains. .. The E. coli donor and P. aeruginosa recipient (106 CFU in 10 μl for each strain) were cospotted on LB agar and incubated at 37°C for 8 h to overnight.

Article Title: Pseudomonas aeruginosa Directly Shunts ?-Oxidation Degradation Intermediates into De Novo Fatty Acid Biosynthesis
Article Snippet: .. Vectors were mobilized in trans using E. coli Stellar cells (Clontech) transformed with the helper plasmid pRK2013 ( ) as the conjugation-proficient donor strain along with different recipient P. aeruginosa strains as has been described previously ( ). .. Merodiploid colonies were confirmed by colony PCR and outgrown for 4 h in 1 ml of LB only at 37°C.

Transformation Assay:

Article Title: Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis
Article Snippet: .. The Trx-lp PCR fragments from S. ratti and T. suis were cloned into pJC45 vector [ ] and IBA 3 plus vector, transformed into Escherichia coli Stellar cells (Clontech, USA) and sequenced (eurofins MWG). ..

Article Title: Fatty Acid Biosynthesis in Pseudomonas aeruginosa Is Initiated by the FabY Class of ?-Ketoacyl Acyl Carrier Protein Synthases
Article Snippet: .. The vectors were mobilized in trans using either E. coli SM10 ( ) or E. coli Stellar cells (Clontech) transformed with the helper plasmid pRK2013 ( ) as the conjugation-proficient donor strains. .. The E. coli donor and P. aeruginosa recipient (106 CFU in 10 μl for each strain) were cospotted on LB agar and incubated at 37°C for 8 h to overnight.

Article Title: Non-lethal inhibition of gut microbial trimethylamine production for the treatment of atherosclerosis
Article Snippet: .. Sequence verified clones were transformed into E. coli Stellar cells (Clontech, Moutainview, CA). .. The ORFs for Desulfovibrio alaskensis G20 cutC/D genes (Ddes_3282 and Ddes_1357, respectively) and their associated intergenic region were codon optimized for expression in E. coli and synthesized by Genscript for cloning into pUC57.

Article Title: Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae
Article Snippet: .. Analysis of DNA from MIDG3446 revealed multiple small plasmids, one of which when transformed into E. coli Stellar cells (Clontech), conferred resistance to florfenicol and chloramphenicol ( ; ). ..

Article Title: Pseudomonas aeruginosa Directly Shunts ?-Oxidation Degradation Intermediates into De Novo Fatty Acid Biosynthesis
Article Snippet: .. Vectors were mobilized in trans using E. coli Stellar cells (Clontech) transformed with the helper plasmid pRK2013 ( ) as the conjugation-proficient donor strain along with different recipient P. aeruginosa strains as has been described previously ( ). .. Merodiploid colonies were confirmed by colony PCR and outgrown for 4 h in 1 ml of LB only at 37°C.

Plasmid Preparation:

Article Title: Coassociation between Group B Streptococcus and Candida albicans Promotes Interactions with Vaginal Epithelium
Article Snippet: .. The resultant amplicon was cloned into vector pHY304 ( ) via XbaI and BamHI sites and propagated in E. coli Stellar cells (Clontech) prior to isolation and electroporation into GBS 515. .. An L. lactis strain expressing BspA had been generated previously , and a similar methodology was employed here to generate an L. lactis strain expressing BspC.

Article Title: Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis
Article Snippet: .. The Trx-lp PCR fragments from S. ratti and T. suis were cloned into pJC45 vector [ ] and IBA 3 plus vector, transformed into Escherichia coli Stellar cells (Clontech, USA) and sequenced (eurofins MWG). ..

Article Title: Fatty Acid Biosynthesis in Pseudomonas aeruginosa Is Initiated by the FabY Class of ?-Ketoacyl Acyl Carrier Protein Synthases
Article Snippet: .. The vectors were mobilized in trans using either E. coli SM10 ( ) or E. coli Stellar cells (Clontech) transformed with the helper plasmid pRK2013 ( ) as the conjugation-proficient donor strains. .. The E. coli donor and P. aeruginosa recipient (106 CFU in 10 μl for each strain) were cospotted on LB agar and incubated at 37°C for 8 h to overnight.

Article Title: Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae
Article Snippet: .. Initially, florfenicol resistance was transferred to E. coli Stellar cells (Clontech) by heat shock with the MIDG3446 plasmid extract, according to the manufacturer's protocol, with selection of transformants on LB agar supplemented with 10 μg/ml florfenicol. ..

Article Title: Pseudomonas aeruginosa Directly Shunts ?-Oxidation Degradation Intermediates into De Novo Fatty Acid Biosynthesis
Article Snippet: .. Vectors were mobilized in trans using E. coli Stellar cells (Clontech) transformed with the helper plasmid pRK2013 ( ) as the conjugation-proficient donor strain along with different recipient P. aeruginosa strains as has been described previously ( ). .. Merodiploid colonies were confirmed by colony PCR and outgrown for 4 h in 1 ml of LB only at 37°C.

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    TaKaRa escherichia coli stellar cells
    Escherichia Coli Stellar Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli stellar cells/product/TaKaRa
    Average 88 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    escherichia coli stellar cells - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

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