escherichia coli novablue de3 competent cells  (Millipore)


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    Millipore escherichia coli novablue de3 competent cells
    Escherichia Coli Novablue De3 Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli novablue de3 competent cells/product/Millipore
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    escherichia coli novablue de3 competent cells - by Bioz Stars, 2020-01
    87/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The identity of the cloned gene was confirmed using the sequencing primers: pMAL-C2X-F5′- TGCGTACTGCGGTGATCAAC-3′ and pMAL-C2X-R 5′-CTGCAAGGCGATTAAGTTGG-3′( http://www.lifetechnologies.com/ ). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: The sequences of the cloned genes were also confirmed by sequencing with primers: pMAL-c2X-F: 5′-TGCGTACTGCGGTGATCAAC-3′ and pMAL-c2X-R: 5′-CTGCAAGGCGATTAAGTTGG-3′. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Amplification:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Agarose Gel Electrophoresis:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: PCR products were purified with a MiniBEST Agarose Gel Extraction Kit (Takara, Dalian, China) and ligated into the pMD19-T simple vector and subsequently transformed into TransT1-competent cells. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Synthesized:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: All the primers used in the present study were designed with Primer Premier 5.0 software (Premier, BC, Canada) and synthesized by the Invitrogen Company (Shanghai, China). .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Purification:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. Isopropyl-β-D -thiogalactopyranoside (IPTG) was employed to induce the expression of CsUGT76F1 .

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. Isopropyl-β- D -thiogalactopyranoside (IPTG) was employed to induce the expression of CsUGT76F1 .

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: Paragraph title: Expression and purification of recombinant CsUGTs ... The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Protein Purification:

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: Paragraph title: Heterologous expression in Escherichia coli and recombinant protein purification ... The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Sequencing:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The identity of the cloned gene was confirmed using the sequencing primers: pMAL-C2X-F5′- TGCGTACTGCGGTGATCAAC-3′ and pMAL-C2X-R 5′-CTGCAAGGCGATTAAGTTGG-3′( http://www.lifetechnologies.com/ ). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: The sequences of the cloned genes were also confirmed by sequencing with primers: pMAL-c2X-F: 5′-TGCGTACTGCGGTGATCAAC-3′ and pMAL-c2X-R: 5′-CTGCAAGGCGATTAAGTTGG-3′. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Affinity Chromatography:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. Isopropyl-β-D -thiogalactopyranoside (IPTG) was employed to induce the expression of CsUGT76F1 .

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. Isopropyl-β- D -thiogalactopyranoside (IPTG) was employed to induce the expression of CsUGT76F1 .

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Expressing:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: Paragraph title: Heterologous Expression in E. coli ... The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: Paragraph title: Heterologous expression in Escherichia coli and recombinant protein purification ... The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: The primer sequences are listed in Supplementary Table S1 at JXB online. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany). .. Recombinant proteins were purified according to the manufacturer’s instruction (New England Biolabs, MA, USA).

    Polymerase Chain Reaction:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: Full-length ORF sequences of CsUGT75L12 were obtained by end-to-end PCR using gene-specific primers (Suppl Table ) and subcloned into the expression vector, pMAL-c2X (New England Biolabs, MA, USA). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: PCR products were purified with a MiniBEST Agarose Gel Extraction Kit (Takara, Dalian, China) and ligated into the pMD19-T simple vector and subsequently transformed into TransT1-competent cells. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Transformation Assay:

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The identity of the cloned gene was confirmed using the sequencing primers: pMAL-C2X-F5′- TGCGTACTGCGGTGATCAAC-3′ and pMAL-C2X-R 5′-CTGCAAGGCGATTAAGTTGG-3′( http://www.lifetechnologies.com/ ). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany). .. The expression strain was grown at 37 °C in 200 mL Luria-Bertani medium containing 100 μg·ml−1 ampicillin and 2 g·L−1 glucose.

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: The primer sequences are listed in Supplementary Table S1 at JXB online. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany). .. Recombinant proteins were purified according to the manufacturer’s instruction (New England Biolabs, MA, USA).

    Recombinant:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The identity of the cloned gene was confirmed using the sequencing primers: pMAL-C2X-F5′- TGCGTACTGCGGTGATCAAC-3′ and pMAL-C2X-R 5′-CTGCAAGGCGATTAAGTTGG-3′( http://www.lifetechnologies.com/ ). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany). .. The expression strain was grown at 37 °C in 200 mL Luria-Bertani medium containing 100 μg·ml−1 ampicillin and 2 g·L−1 glucose.

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: Paragraph title: Expression and purification of recombinant CsUGTs ... The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Plasmid Preparation:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: Full-length ORF sequences of CsUGT75L12 were obtained by end-to-end PCR using gene-specific primers (Suppl Table ) and subcloned into the expression vector, pMAL-c2X (New England Biolabs, MA, USA). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: The open reading frames of the CsUGTs were subcloned into the expression vector pMAL-c2X (New England Biolabs, MA, USA). .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Software:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: All the primers used in the present study were designed with Primer Premier 5.0 software (Premier, BC, Canada) and synthesized by the Invitrogen Company (Shanghai, China). .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

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    Millipore escherichia coli de3 novablue cells
    Escherichia Coli De3 Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli de3 novablue cells/product/Millipore
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli de3 novablue cells - by Bioz Stars, 2020-01
    82/100 stars
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