escherichia coli novablue de3 competent cells  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Millipore escherichia coli novablue de3 competent cells
    Escherichia Coli Novablue De3 Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli novablue de3 competent cells/product/Millipore
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    escherichia coli novablue de3 competent cells - by Bioz Stars, 2020-04
    91/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: The sequences of the cloned genes were also confirmed by sequencing with primers: pMAL-c2X-F: 5′-TGCGTACTGCGGTGATCAAC-3′ and pMAL-c2X-R: 5′-CTGCAAGGCGATTAAGTTGG-3′. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The identity of the cloned gene was confirmed using the sequencing primers: pMAL-C2X-F5′- TGCGTACTGCGGTGATCAAC-3′ and pMAL-C2X-R 5′-CTGCAAGGCGATTAAGTTGG-3′( http://www.lifetechnologies.com/ ). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Functional Analysis of 3-Dehydroquinate Dehydratase/Shikimate Dehydrogenases Involved in Shikimate Pathway in Camellia sinensis
    Article Snippet: .. CsDQD /SDH Expression in Escherichia coli and Purification of Recombinant Proteins CsDQD/SDH ORFs were cloned into the pMAL-c2x (New England Biolabs, MA, USA) expression vector and then expressed in E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach, Germany) to express recombinant proteins, and the proteins purified according to the manufacturer’s protocol (New England Biolabs). .. The recombinant E. coli strains expressing Cs DQD/SDHs were shaken in Luria–Bertani (LB) medium (1 L of LB culture medium containing 5 g of yeast extract, 10 g of tryptone, and 10 g of NaCl, adjusted to pH 7.0 before sterilization) containing 1 µL·ml−1 of ampicillin to an OD600 of 0.6, and the cells were shaken at 37°C at 220 rpm.

    Centrifugation:

    Article Title: Functional Analysis of 3-Dehydroquinate Dehydratase/Shikimate Dehydrogenases Involved in Shikimate Pathway in Camellia sinensis
    Article Snippet: CsDQD /SDH Expression in Escherichia coli and Purification of Recombinant Proteins CsDQD/SDH ORFs were cloned into the pMAL-c2x (New England Biolabs, MA, USA) expression vector and then expressed in E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach, Germany) to express recombinant proteins, and the proteins purified according to the manufacturer’s protocol (New England Biolabs). .. Thereafter, 1 mmol·L−1 of IPTG was added to the culture medium, and protein expression in strains was induced at 24°C for 24 h. After centrifugation at 7,200 rpm for 15 min, supernatants were discarded, and the precipitates were resuspended in column buffer (200 mM of NaCl, 20 mM of Tris, 1 mM of EDTA, and 1 mM of DTT, pH 7.5).

    Affinity Chromatography:

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany). .. Recombinant proteins were purified by affinity chromatography using amylase resin (New England Biolabs, MA, USA).

    Agarose Gel Electrophoresis:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: PCR products were purified with a MiniBEST Agarose Gel Extraction Kit (Takara, Dalian, China) and ligated into the pMD19-T simple vector and subsequently transformed into TransT1-competent cells. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Synthesized:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: All the primers used in the present study were designed with Primer Premier 5.0 software (Premier, BC, Canada) and synthesized by the Invitrogen Company (Shanghai, China). .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Purification:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: Paragraph title: Expression and purification of recombinant CsUGTs ... The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany). .. Recombinant proteins were purified by affinity chromatography using amylase resin (New England Biolabs, MA, USA).

    Article Title: Functional Analysis of 3-Dehydroquinate Dehydratase/Shikimate Dehydrogenases Involved in Shikimate Pathway in Camellia sinensis
    Article Snippet: .. CsDQD /SDH Expression in Escherichia coli and Purification of Recombinant Proteins CsDQD/SDH ORFs were cloned into the pMAL-c2x (New England Biolabs, MA, USA) expression vector and then expressed in E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach, Germany) to express recombinant proteins, and the proteins purified according to the manufacturer’s protocol (New England Biolabs). .. The recombinant E. coli strains expressing Cs DQD/SDHs were shaken in Luria–Bertani (LB) medium (1 L of LB culture medium containing 5 g of yeast extract, 10 g of tryptone, and 10 g of NaCl, adjusted to pH 7.0 before sterilization) containing 1 µL·ml−1 of ampicillin to an OD600 of 0.6, and the cells were shaken at 37°C at 220 rpm.

    Protein Purification:

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: Paragraph title: Heterologous expression in Escherichia coli and recombinant protein purification ... The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Sequencing:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: The sequences of the cloned genes were also confirmed by sequencing with primers: pMAL-c2X-F: 5′-TGCGTACTGCGGTGATCAAC-3′ and pMAL-c2X-R: 5′-CTGCAAGGCGATTAAGTTGG-3′. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: The identity of the cloned gene was confirmed using the sequencing primers: pMAL-C2X-F5′- TGCGTACTGCGGTGATCAAC-3′ and pMAL-C2X-R 5′-CTGCAAGGCGATTAAGTTGG-3′( http://www.lifetechnologies.com/ ). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: Heterologous Expression in E. coli The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Amplification:

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: Heterologous Expression in E. coli The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Expressing:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany). .. Recombinant proteins were purified according to the manufacturer’s instruction (New England Biolabs, MA, USA).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: Paragraph title: Heterologous expression in Escherichia coli and recombinant protein purification ... The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: Paragraph title: Heterologous Expression in E. coli ... The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Functional Analysis of 3-Dehydroquinate Dehydratase/Shikimate Dehydrogenases Involved in Shikimate Pathway in Camellia sinensis
    Article Snippet: .. CsDQD /SDH Expression in Escherichia coli and Purification of Recombinant Proteins CsDQD/SDH ORFs were cloned into the pMAL-c2x (New England Biolabs, MA, USA) expression vector and then expressed in E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach, Germany) to express recombinant proteins, and the proteins purified according to the manufacturer’s protocol (New England Biolabs). .. The recombinant E. coli strains expressing Cs DQD/SDHs were shaken in Luria–Bertani (LB) medium (1 L of LB culture medium containing 5 g of yeast extract, 10 g of tryptone, and 10 g of NaCl, adjusted to pH 7.0 before sterilization) containing 1 µL·ml−1 of ampicillin to an OD600 of 0.6, and the cells were shaken at 37°C at 220 rpm.

    Polymerase Chain Reaction:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: PCR products were purified with a MiniBEST Agarose Gel Extraction Kit (Takara, Dalian, China) and ligated into the pMD19-T simple vector and subsequently transformed into TransT1-competent cells. .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: Heterologous expression in Escherichia coli and recombinant protein purification Full-length ORF sequences of CsUGT75L12 were obtained by end-to-end PCR using gene-specific primers (Suppl Table ) and subcloned into the expression vector, pMAL-c2X (New England Biolabs, MA, USA). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: Heterologous Expression in E. coli The coding sequence of CsUGT76F1 was amplified by PCR with forward and reverse primers ( Supplementary Table ), following which the PCR product was sub-cloned into the pMAL-c2X expression vector with a maltose-tag (New England Biolabs, Ipswich, MA, United States). .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany).

    Transformation Assay:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany). .. Recombinant proteins were purified according to the manufacturer’s instruction (New England Biolabs, MA, USA).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany). .. The expression strain was grown at 37 °C in 200 mL Luria-Bertani medium containing 100 μg·ml−1 ampicillin and 2 g·L−1 glucose.

    Recombinant:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: Paragraph title: Expression and purification of recombinant CsUGTs ... The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany). .. The expression strain was grown at 37 °C in 200 mL Luria-Bertani medium containing 100 μg·ml−1 ampicillin and 2 g·L−1 glucose.

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Functional Analysis of 3-Dehydroquinate Dehydratase/Shikimate Dehydrogenases Involved in Shikimate Pathway in Camellia sinensis
    Article Snippet: .. CsDQD /SDH Expression in Escherichia coli and Purification of Recombinant Proteins CsDQD/SDH ORFs were cloned into the pMAL-c2x (New England Biolabs, MA, USA) expression vector and then expressed in E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach, Germany) to express recombinant proteins, and the proteins purified according to the manufacturer’s protocol (New England Biolabs). .. The recombinant E. coli strains expressing Cs DQD/SDHs were shaken in Luria–Bertani (LB) medium (1 L of LB culture medium containing 5 g of yeast extract, 10 g of tryptone, and 10 g of NaCl, adjusted to pH 7.0 before sterilization) containing 1 µL·ml−1 of ampicillin to an OD600 of 0.6, and the cells were shaken at 37°C at 220 rpm.

    Plasmid Preparation:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: The open reading frames of the CsUGTs were subcloned into the expression vector pMAL-c2X (New England Biolabs, MA, USA). .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Article Title: Identification of a Flavonoid Glucosyltransferase Involved in 7-OH Site Glycosylation in Tea plants (Camellia sinensis)
    Article Snippet: Heterologous expression in Escherichia coli and recombinant protein purification Full-length ORF sequences of CsUGT75L12 were obtained by end-to-end PCR using gene-specific primers (Suppl Table ) and subcloned into the expression vector, pMAL-c2X (New England Biolabs, MA, USA). .. The recombinant pMAL-c2X-CsUGT75L12 was transformed into Escherichia coli Novablue (DE3) Competent Cells (Novagen, Schwalbach, Germany).

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Functional Characterization of a Flavonoid Glycosyltransferase in Sweet Orange (Citrus sinensis)
    Article Snippet: .. The recombinant plasmid was introduced into E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach am Taunus, Germany). .. The positive clones were identified in 5 mL of lysogeny broth with 80 mg/L ampicillin for 8–12 h at 37°C.

    Article Title: Functional Analysis of 3-Dehydroquinate Dehydratase/Shikimate Dehydrogenases Involved in Shikimate Pathway in Camellia sinensis
    Article Snippet: .. CsDQD /SDH Expression in Escherichia coli and Purification of Recombinant Proteins CsDQD/SDH ORFs were cloned into the pMAL-c2x (New England Biolabs, MA, USA) expression vector and then expressed in E. coli NovaBlue (DE3) competent cells (Novagen, Schwalbach, Germany) to express recombinant proteins, and the proteins purified according to the manufacturer’s protocol (New England Biolabs). .. The recombinant E. coli strains expressing Cs DQD/SDHs were shaken in Luria–Bertani (LB) medium (1 L of LB culture medium containing 5 g of yeast extract, 10 g of tryptone, and 10 g of NaCl, adjusted to pH 7.0 before sterilization) containing 1 µL·ml−1 of ampicillin to an OD600 of 0.6, and the cells were shaken at 37°C at 220 rpm.

    Software:

    Article Title: Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)
    Article Snippet: All the primers used in the present study were designed with Primer Premier 5.0 software (Premier, BC, Canada) and synthesized by the Invitrogen Company (Shanghai, China). .. The pMAL-c2X expression plasmids harbouring the CsUGT genes were transformed into Escherichia coli Novablue (DE3) competent cells (Novagen, Schwalbach, Germany).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89
    Millipore escherichia coli de3 novablue cells
    Escherichia Coli De3 Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli de3 novablue cells/product/Millipore
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli de3 novablue cells - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

    Image Search Results