escherichia coli novablue competent cells  (Millipore)


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    Structured Review

    Millipore escherichia coli novablue competent cells
    Escherichia Coli Novablue Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli novablue competent cells/product/Millipore
    Average 84 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    escherichia coli novablue competent cells - by Bioz Stars, 2020-01
    84/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The gene encoding the ecFabI protein was cloned via the ligation-independent cloning (LIC) strategy into the expression vector pET-30 Ek/LIC using a LIC cloning kit according to the manufacturer’s instructions (Novagen Technical Bulletin No. 163). .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed.

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: The new target constructs—HEβ1314 (residues 1585–1798), HEβ1415 (residues 1685–1910), HEβ1516 (residues 1799–2010), and HEβ1315 (residues 1583–1906)—were PCR amplified for cloning into the ligation-independent vector pMCSG7 ( ). .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences). .. Selecting multiple clones minimized the false-positive rate of any rare chimeric product that had the same size as a true allele.

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: .. Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Positive clones were verified using the colony PCR method with the adaptor-specific primers N1 (5′-TCGAGCGGCCGCCCGGGCAGGT-3′) and N2R (5′-AGCGTGGTCGCGGCCGAGGT-3′), and the plasmids were isolated for sequencing using the High Pure Plasmid Isolation Kit (Roche Applied Sciences).

    Article Title: Reconstruction of metabolic pathway for isobutanol production in Escherichia coli
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning. .. Polymerase chain reaction (PCR) was performed using the KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan), and the primer pairs are listed in Additional file : Table S1.

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The PCR product was gel-purified, treated with T4 DNA polymerase and annealed via ligation-independent cloning (LIC) to the AVA0421 vector, a T7 expression vector containing a cleavable six-histidine tag at the N-terminus (Choi et al. , 2011 ). .. The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing.

    Article Title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance
    Article Snippet: .. Cloning and confirmation of the positive clones The secondary PCR product of the SSH method was purified with the PCR Product Purification Kit (Roche Applied Sciences, Indianapolis, IN, USA), cloned into pUC19 plasmid vectors and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Randomly selected positive colonies were first confirmed with a colony PCR, using N1 and N2R primers (Table ).

    Article Title: Engineering a synthetic pathway for maleate in Escherichia coli
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning. .. Polymerase chain reaction (PCR) was performed using the KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan) and the appropriate primer pairs.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: Paragraph title: 2.1. Cloning, expression and purification ... The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media.

    Centrifugation:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter. .. When A 595 reached 0.6, expression of the enzyme was induced by IPTG to a final concentration of 1 mM, and the cells were further cultivated at 18°C for 20 h. The cells were then harvested by centrifugation and resuspended in buffer A.

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing. .. The culture was grown for 24 h at 298 K and was then incubated for a further 60 h at 288 K. The cells were collected by centrifugation at 4000 g for 20 min at 277 K. The cell paste was stored frozen at 193 K. The frozen E. coli cell-paste pellet was lysed in 200 ml lysis buffer (0.3 M NaCl, 20 m M HEPES pH 7.4, 5% glycerol, 30 m M imidazole, 0.5% CHAPS, 2 m M MgCl2 ) with lysozyme, five tablets of Roche protease-inhibitor cocktail and 250 µl 14.3 M β-mercaptoethanol.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. N-terminally hexahistidine-tagged Rph was expressed from E. coli BL21 (DE3)/pETBaRph by overnight growth in kanamycin-supplemented autoinduction media (Studier, 2005 ) at 310 K. Cells from 0.5 l culture were harvested by centrifugation at 5000 rev min−1 for 15 min. Pelletted cells were resuspended in buffer A (20 m M Na2 HPO4 , 0.5 M NaCl, 10 m M imidazole pH 7.5) and lysed by sonication.

    Amplification:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The amplified DNA fragment was treated with T4 DNA polymerase in the presence of dATP and annealed to linearized pET-30 Ek/LIC vector (Novagen Technical Bulletin No. 163). .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed.

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: Sizes of the PCR products were verified by agarose gel electrophoresis and the amplified DNA products purified (Qiagen Gel Extraction Kit). .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences). .. Selecting multiple clones minimized the false-positive rate of any rare chimeric product that had the same size as a true allele.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: The rph coding sequence was amplified by PCR from B. anthracis Ames genomic DNA using BA4715F (5′-CACCACCACCACATG­CGAGTAGATGGTAGAGAGAAAA-3′) as a forward primer and BA4715R (5′-GAGGAGAAGGCGCGTTACTACTCTATATGAG­ATACGATGTCACCTAAC-3′) as a reverse primer and cloned using a ligation-independent cloning method (Alzari et al. , 2006 ; Au et al. , 2006 ; Fogg & Wilkinson, 2008 ). .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media.

    Construct:

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: Paragraph title: Preparation of spectrin constructs ... The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: .. Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Positive clones were verified using the colony PCR method with the adaptor-specific primers N1 (5′-TCGAGCGGCCGCCCGGGCAGGT-3′) and N2R (5′-AGCGTGGTCGCGGCCGAGGT-3′), and the plasmids were isolated for sequencing using the High Pure Plasmid Isolation Kit (Roche Applied Sciences).

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: .. The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing. .. After transformation into the expression host, a starter culture from a single colony in LB broth was grown for ∼18 h at 310 K. Protein was expressed in a LEX bioreactor in the presence of 50 µg ml−1 ampicillin, 50 µg ml−1 carbenicillin and 34 µg ml−1 chloramphenicol in 2 l ZYP-5052 auto-induction medium (Studier, 2005 ).

    Incubation:

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing. .. The culture was grown for 24 h at 298 K and was then incubated for a further 60 h at 288 K. The cells were collected by centrifugation at 4000 g for 20 min at 277 K. The cell paste was stored frozen at 193 K. The frozen E. coli cell-paste pellet was lysed in 200 ml lysis buffer (0.3 M NaCl, 20 m M HEPES pH 7.4, 5% glycerol, 30 m M imidazole, 0.5% CHAPS, 2 m M MgCl2 ) with lysozyme, five tablets of Roche protease-inhibitor cocktail and 250 µl 14.3 M β-mercaptoethanol.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. Kanamycin-resistant colonies were picked the following day and used to inoculate overnight cultures, from which recombinant plasmids were purified.

    Expressing:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter. .. When A 595 reached 0.6, expression of the enzyme was induced by IPTG to a final concentration of 1 mM, and the cells were further cultivated at 18°C for 20 h. The cells were then harvested by centrifugation and resuspended in buffer A.

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The gene encoding the ecFabI protein was cloned via the ligation-independent cloning (LIC) strategy into the expression vector pET-30 Ek/LIC using a LIC cloning kit according to the manufacturer’s instructions (Novagen Technical Bulletin No. 163). .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed.

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: .. The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing. .. After transformation into the expression host, a starter culture from a single colony in LB broth was grown for ∼18 h at 310 K. Protein was expressed in a LEX bioreactor in the presence of 50 µg ml−1 ampicillin, 50 µg ml−1 carbenicillin and 34 µg ml−1 chloramphenicol in 2 l ZYP-5052 auto-induction medium (Studier, 2005 ).

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: Paragraph title: 2.1. Cloning, expression and purification ... The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media.

    Modification:

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: The resulting fragment was treated with T4 DNA polymerase in the presence of dATP to generate single-stranded DNA overhangs complementary to those present on a modified pET28a vector cut with Bse RI and then similarly treated with T4 DNA polymerase in the presence of dTTP. .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media.

    Transformation Assay:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed. .. The recombinant plasmid (pET30-ecFabI) was isolated and retransformed into the expression host E. coli BL21 (DE3) (Supplementary Table S1).

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin. ..

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences). .. Selecting multiple clones minimized the false-positive rate of any rare chimeric product that had the same size as a true allele.

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: .. Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Positive clones were verified using the colony PCR method with the adaptor-specific primers N1 (5′-TCGAGCGGCCGCCCGGGCAGGT-3′) and N2R (5′-AGCGTGGTCGCGGCCGAGGT-3′), and the plasmids were isolated for sequencing using the High Pure Plasmid Isolation Kit (Roche Applied Sciences).

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: .. The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing. .. After transformation into the expression host, a starter culture from a single colony in LB broth was grown for ∼18 h at 310 K. Protein was expressed in a LEX bioreactor in the presence of 50 µg ml−1 ampicillin, 50 µg ml−1 carbenicillin and 34 µg ml−1 chloramphenicol in 2 l ZYP-5052 auto-induction medium (Studier, 2005 ).

    Article Title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance
    Article Snippet: .. Cloning and confirmation of the positive clones The secondary PCR product of the SSH method was purified with the PCR Product Purification Kit (Roche Applied Sciences, Indianapolis, IN, USA), cloned into pUC19 plasmid vectors and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Randomly selected positive colonies were first confirmed with a colony PCR, using N1 and N2R primers (Table ).

    Ligation:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The gene encoding the ecFabI protein was cloned via the ligation-independent cloning (LIC) strategy into the expression vector pET-30 Ek/LIC using a LIC cloning kit according to the manufacturer’s instructions (Novagen Technical Bulletin No. 163). .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed.

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: The new target constructs—HEβ1314 (residues 1585–1798), HEβ1415 (residues 1685–1910), HEβ1516 (residues 1799–2010), and HEβ1315 (residues 1583–1906)—were PCR amplified for cloning into the ligation-independent vector pMCSG7 ( ). .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The PCR product was gel-purified, treated with T4 DNA polymerase and annealed via ligation-independent cloning (LIC) to the AVA0421 vector, a T7 expression vector containing a cleavable six-histidine tag at the N-terminus (Choi et al. , 2011 ). .. The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: The rph coding sequence was amplified by PCR from B. anthracis Ames genomic DNA using BA4715F (5′-CACCACCACCACATG­CGAGTAGATGGTAGAGAGAAAA-3′) as a forward primer and BA4715R (5′-GAGGAGAAGGCGCGTTACTACTCTATATGAG­ATACGATGTCACCTAAC-3′) as a reverse primer and cloned using a ligation-independent cloning method (Alzari et al. , 2006 ; Au et al. , 2006 ; Fogg & Wilkinson, 2008 ). .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media.

    Protease Inhibitor:

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing. .. The culture was grown for 24 h at 298 K and was then incubated for a further 60 h at 288 K. The cells were collected by centrifugation at 4000 g for 20 min at 277 K. The cell paste was stored frozen at 193 K. The frozen E. coli cell-paste pellet was lysed in 200 ml lysis buffer (0.3 M NaCl, 20 m M HEPES pH 7.4, 5% glycerol, 30 m M imidazole, 0.5% CHAPS, 2 m M MgCl2 ) with lysozyme, five tablets of Roche protease-inhibitor cocktail and 250 µl 14.3 M β-mercaptoethanol.

    DNA Sequencing:

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Single direction DNA sequencing was performed using the BigDye terminator v3.1 sequencing kit and a 3730xl automated sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance
    Article Snippet: Cloning and confirmation of the positive clones The secondary PCR product of the SSH method was purified with the PCR Product Purification Kit (Roche Applied Sciences, Indianapolis, IN, USA), cloned into pUC19 plasmid vectors and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Plasmids from the confirmed positive clones were isolated by the High Pure Plasmid Isolation Kit (Roche Applied Sciences, Indianapolis, IN, USA) and used in single direction DNA sequencing with the BigDye Terminator Version 3.1 Sequencing Kit and a 3730xl Automated Sequencer (Applied Biosystems, Foster City, CA, USA).

    Sequencing:

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: Paragraph title: Amplification and sequencing of the zinc finger PRDM9 array ... Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences).

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Positive clones were verified using the colony PCR method with the adaptor-specific primers N1 (5′-TCGAGCGGCCGCCCGGGCAGGT-3′) and N2R (5′-AGCGTGGTCGCGGCCGAGGT-3′), and the plasmids were isolated for sequencing using the High Pure Plasmid Isolation Kit (Roche Applied Sciences).

    Article Title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance
    Article Snippet: Cloning and confirmation of the positive clones The secondary PCR product of the SSH method was purified with the PCR Product Purification Kit (Roche Applied Sciences, Indianapolis, IN, USA), cloned into pUC19 plasmid vectors and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Plasmids from the confirmed positive clones were isolated by the High Pure Plasmid Isolation Kit (Roche Applied Sciences, Indianapolis, IN, USA) and used in single direction DNA sequencing with the BigDye Terminator Version 3.1 Sequencing Kit and a 3730xl Automated Sequencer (Applied Biosystems, Foster City, CA, USA).

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: The rph coding sequence was amplified by PCR from B. anthracis Ames genomic DNA using BA4715F (5′-CACCACCACCACATG­CGAGTAGATGGTAGAGAGAAAA-3′) as a forward primer and BA4715R (5′-GAGGAGAAGGCGCGTTACTACTCTATATGAG­ATACGATGTCACCTAAC-3′) as a reverse primer and cloned using a ligation-independent cloning method (Alzari et al. , 2006 ; Au et al. , 2006 ; Fogg & Wilkinson, 2008 ). .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media.

    Sonication:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed. .. Cells were lysed by treatment with lysozyme and sonication in 50 m M Tris, 150 m M NaCl pH 7.5 with 10 µg ml−1 DNase, 10 µg ml−1 RNase and 1 m M PMSF.

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing. .. The suspension was sonicated for 45 min with a pulse frequency of 5 s on, 10 s off.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. N-terminally hexahistidine-tagged Rph was expressed from E. coli BL21 (DE3)/pETBaRph by overnight growth in kanamycin-supplemented autoinduction media (Studier, 2005 ) at 310 K. Cells from 0.5 l culture were harvested by centrifugation at 5000 rev min−1 for 15 min. Pelletted cells were resuspended in buffer A (20 m M Na2 HPO4 , 0.5 M NaCl, 10 m M imidazole pH 7.5) and lysed by sonication.

    Recombinant:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: Paragraph title: Production and purification of the recombinant β-galactosidase. ... E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter.

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed. .. The recombinant plasmid (pET30-ecFabI) was isolated and retransformed into the expression host E. coli BL21 (DE3) (Supplementary Table S1).

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin. ..

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. Kanamycin-resistant colonies were picked the following day and used to inoculate overnight cultures, from which recombinant plasmids were purified.

    Isolation:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed. .. The recombinant plasmid (pET30-ecFabI) was isolated and retransformed into the expression host E. coli BL21 (DE3) (Supplementary Table S1).

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Positive clones were verified using the colony PCR method with the adaptor-specific primers N1 (5′-TCGAGCGGCCGCCCGGGCAGGT-3′) and N2R (5′-AGCGTGGTCGCGGCCGAGGT-3′), and the plasmids were isolated for sequencing using the High Pure Plasmid Isolation Kit (Roche Applied Sciences).

    Article Title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance
    Article Snippet: Cloning and confirmation of the positive clones The secondary PCR product of the SSH method was purified with the PCR Product Purification Kit (Roche Applied Sciences, Indianapolis, IN, USA), cloned into pUC19 plasmid vectors and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Plasmids from the confirmed positive clones were isolated by the High Pure Plasmid Isolation Kit (Roche Applied Sciences, Indianapolis, IN, USA) and used in single direction DNA sequencing with the BigDye Terminator Version 3.1 Sequencing Kit and a 3730xl Automated Sequencer (Applied Biosystems, Foster City, CA, USA).

    Purification:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: Paragraph title: Production and purification of the recombinant β-galactosidase. ... E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter.

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: Sizes of the PCR products were verified by agarose gel electrophoresis and the amplified DNA products purified (Qiagen Gel Extraction Kit). .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: .. Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Positive clones were verified using the colony PCR method with the adaptor-specific primers N1 (5′-TCGAGCGGCCGCCCGGGCAGGT-3′) and N2R (5′-AGCGTGGTCGCGGCCGAGGT-3′), and the plasmids were isolated for sequencing using the High Pure Plasmid Isolation Kit (Roche Applied Sciences).

    Article Title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance
    Article Snippet: .. Cloning and confirmation of the positive clones The secondary PCR product of the SSH method was purified with the PCR Product Purification Kit (Roche Applied Sciences, Indianapolis, IN, USA), cloned into pUC19 plasmid vectors and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Randomly selected positive colonies were first confirmed with a colony PCR, using N1 and N2R primers (Table ).

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: Paragraph title: 2.1. Cloning, expression and purification ... The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media.

    Polymerase Chain Reaction:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The fabI gene was amplified via PCR using genomic DNA from E. coli strain MG1655 as the template. .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed.

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: Sizes of the PCR products were verified by agarose gel electrophoresis and the amplified DNA products purified (Qiagen Gel Extraction Kit). .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences). .. Selecting multiple clones minimized the false-positive rate of any rare chimeric product that had the same size as a true allele.

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: .. Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Positive clones were verified using the colony PCR method with the adaptor-specific primers N1 (5′-TCGAGCGGCCGCCCGGGCAGGT-3′) and N2R (5′-AGCGTGGTCGCGGCCGAGGT-3′), and the plasmids were isolated for sequencing using the High Pure Plasmid Isolation Kit (Roche Applied Sciences).

    Article Title: Reconstruction of metabolic pathway for isobutanol production in Escherichia coli
    Article Snippet: E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning. .. Polymerase chain reaction (PCR) was performed using the KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan), and the primer pairs are listed in Additional file : Table S1.

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The PCR product was gel-purified, treated with T4 DNA polymerase and annealed via ligation-independent cloning (LIC) to the AVA0421 vector, a T7 expression vector containing a cleavable six-histidine tag at the N-terminus (Choi et al. , 2011 ). .. The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing.

    Article Title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance
    Article Snippet: .. Cloning and confirmation of the positive clones The secondary PCR product of the SSH method was purified with the PCR Product Purification Kit (Roche Applied Sciences, Indianapolis, IN, USA), cloned into pUC19 plasmid vectors and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Randomly selected positive colonies were first confirmed with a colony PCR, using N1 and N2R primers (Table ).

    Article Title: Engineering a synthetic pathway for maleate in Escherichia coli
    Article Snippet: E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning. .. Polymerase chain reaction (PCR) was performed using the KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan) and the appropriate primer pairs.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. Kanamycin-resistant colonies were picked the following day and used to inoculate overnight cultures, from which recombinant plasmids were purified.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: Chimeras, PRDM7 , and primer-primer dimers were typically seen as fainter ladder-like bands beneath the main band when analyzed via PAGE. .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences).

    Gel Extraction:

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: Sizes of the PCR products were verified by agarose gel electrophoresis and the amplified DNA products purified (Qiagen Gel Extraction Kit). .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Plasmid Preparation:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter. .. When A 595 reached 0.6, expression of the enzyme was induced by IPTG to a final concentration of 1 mM, and the cells were further cultivated at 18°C for 20 h. The cells were then harvested by centrifugation and resuspended in buffer A.

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The amplified DNA fragment was treated with T4 DNA polymerase in the presence of dATP and annealed to linearized pET-30 Ek/LIC vector (Novagen Technical Bulletin No. 163). .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed.

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: Exonuclease treatment for incorporation into the vector was performed according to Stols et al. ( ). .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy
    Article Snippet: .. Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Positive clones were verified using the colony PCR method with the adaptor-specific primers N1 (5′-TCGAGCGGCCGCCCGGGCAGGT-3′) and N2R (5′-AGCGTGGTCGCGGCCGAGGT-3′), and the plasmids were isolated for sequencing using the High Pure Plasmid Isolation Kit (Roche Applied Sciences).

    Article Title: Reconstruction of metabolic pathway for isobutanol production in Escherichia coli
    Article Snippet: Paragraph title: Strains and plasmid construction ... E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning.

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The PCR product was gel-purified, treated with T4 DNA polymerase and annealed via ligation-independent cloning (LIC) to the AVA0421 vector, a T7 expression vector containing a cleavable six-histidine tag at the N-terminus (Choi et al. , 2011 ). .. The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing.

    Article Title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance
    Article Snippet: .. Cloning and confirmation of the positive clones The secondary PCR product of the SSH method was purified with the PCR Product Purification Kit (Roche Applied Sciences, Indianapolis, IN, USA), cloned into pUC19 plasmid vectors and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA). .. Randomly selected positive colonies were first confirmed with a colony PCR, using N1 and N2R primers (Table ).

    Article Title: Engineering a synthetic pathway for maleate in Escherichia coli
    Article Snippet: Paragraph title: Strains and plasmid construction ... E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. Kanamycin-resistant colonies were picked the following day and used to inoculate overnight cultures, from which recombinant plasmids were purified.

    Positron Emission Tomography:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: The amplified DNA fragment was treated with T4 DNA polymerase in the presence of dATP and annealed to linearized pET-30 Ek/LIC vector (Novagen Technical Bulletin No. 163). .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed.

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin. .. Cloning and preparation of HEβ89 in pET-23d and CBα1617 in pET-3d were completed as previously ( , , ).

    Agarose Gel Electrophoresis:

    Article Title: MOLECULAR EPITOPES OF THE ANKYRIN-SPECTRIN INTERACTION
    Article Snippet: Sizes of the PCR products were verified by agarose gel electrophoresis and the amplified DNA products purified (Qiagen Gel Extraction Kit). .. The recombinant plasmids were transformed into Escherichia coli NovaBlue competent cells (Novagen) by heat shock and plated on LB agar with 100 µg/mL ampicillin.

    Produced:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: The recombinant β-galactosidase was produced using the expression T7 system ( ). .. E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter.

    Concentration Assay:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter. .. When A 595 reached 0.6, expression of the enzyme was induced by IPTG to a final concentration of 1 mM, and the cells were further cultivated at 18°C for 20 h. The cells were then harvested by centrifugation and resuspended in buffer A.

    Lysis:

    Article Title: Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP
    Article Snippet: The LIC-annealed construct was propagated in Escherichia coli NovaBlue competent cells (EMD Biosciences) and subsequently transformed into the E. coli Rosetta Oxford BL21(DE3)R3 expression host strain for expression testing. .. The culture was grown for 24 h at 298 K and was then incubated for a further 60 h at 288 K. The cells were collected by centrifugation at 4000 g for 20 min at 277 K. The cell paste was stored frozen at 193 K. The frozen E. coli cell-paste pellet was lysed in 200 ml lysis buffer (0.3 M NaCl, 20 m M HEPES pH 7.4, 5% glycerol, 30 m M imidazole, 0.5% CHAPS, 2 m M MgCl2 ) with lysozyme, five tablets of Roche protease-inhibitor cocktail and 250 µl 14.3 M β-mercaptoethanol.

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    Millipore escherichia coli novablue competent cells
    Escherichia Coli Novablue Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli novablue competent cells/product/Millipore
    Average 84 stars, based on 4 article reviews
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