escherichia coli jm109 high efficiency competent cells  (Promega)

 
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    Name:
    JM109 Competent Cells
    Description:
    E coli strain ready for transformation offers blue white selection
    Catalog Number:
    l2005
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Cloning DNA Markers Bacterial Strains and Competent Cells
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    Structured Review

    Promega escherichia coli jm109 high efficiency competent cells
    E coli strain ready for transformation offers blue white selection
    https://www.bioz.com/result/escherichia coli jm109 high efficiency competent cells/product/Promega
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli jm109 high efficiency competent cells - by Bioz Stars, 2020-08
    99/100 stars

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    Ligation:

    Article Title: RAB10 Interacts with the Male Germ Cell-Specific GTPase-Activating Protein during Mammalian Spermiogenesis
    Article Snippet: .. Finally, ligation and transformation was performed using T4 DNA ligase (M0202; New England Biolabs, Ipswich, MA, USA) and JM109 competent cells, respectively (L2001; Promega, Fitchburg, WI, USA) [ , ]. .. Subsequently, NTERA-2 cl.D1 (NT2D1) cells (ATCC, Manassas, VA, USA), a pluripotent human testicular embryonal carcinoma cell line, were transfected with plasmids using Lipofectamine 2000 (Cat No.: 11668; Invitrogen, Carlsbad, CA, USA).

    Construct:

    Article Title: pELMO, an optimised in-house cloning vector
    Article Snippet: .. The resulting constructs were transformed in E. coli JM109 competent cells (Promega). .. Clones from each plate were randomly chosen and analysed by colony PCR after 15 h incubation at 37 °C with Np21+/Np6+, PvARNP-D/PvARNP-R and Pvron4intdir/Pvron4intrev primer sets (Table ).

    Article Title: Bacterial community composition of biofilms in milking machines of two dairy farms assessed by a combination of culture-dependent and –independent methods
    Article Snippet: .. The purified PCR products were used to construct clone libraries with the pGEM-T vector system and were transferred into competent E . coli JM109 cells as specified by the manufacturer (Promega Corp., Madison, WI). .. Inserts of randomly selected clones were amplified using the primer pair M13F and M13R [ ].

    Purification:

    Article Title: Identification and Localization of an Arachidonic Acid-Sensitive Potassium Channel in the Cochlea
    Article Snippet: .. The purified c Shal fragment was ligated into the alkaline phosphatase (AP)-treated, Bam HI-cut pcDNA3.1 (+) vector and transformed into JM109-competent cells (Promega, Madison, WI). .. Transformants were screened for ampicillin-resistant recombinant plasmids and extracted using the QIAfilter Plasmid Midi kit (Qiagen) according to the manufacturer's protocol.

    Article Title: Shifts from glucose to certain secondary carbon-sources result in activation of the extracytoplasmic function sigma factor ?E in Salmonella enterica serovar Typhimurium
    Article Snippet: .. The 1·4 kb PCR product was purified and ligated into pGEM-T Easy (Promega), and E. coli JM109 competent cells (Promega) were transformed with this, following the manufacturer’s protocols. .. Plasmid minipreps (Wizard Plus Minipreps; Promega) from the ampicillin-resistant transformants were screened by PCR using primers PR48 and PR29 ( ) to determine that the insert was oriented in the same direction as the lacZ gene in order to facilitate cloning into pBAD33.

    Article Title: Bacterial community composition of biofilms in milking machines of two dairy farms assessed by a combination of culture-dependent and –independent methods
    Article Snippet: .. The purified PCR products were used to construct clone libraries with the pGEM-T vector system and were transferred into competent E . coli JM109 cells as specified by the manufacturer (Promega Corp., Madison, WI). .. Inserts of randomly selected clones were amplified using the primer pair M13F and M13R [ ].

    Incubation:

    Article Title: Synbiotic therapy (Bifidobacterium longum/Synergy 1) initiates resolution of inflammation in patients with active ulcerative colitis: a randomised controlled pilot trial
    Article Snippet: .. JM109 competent E coli (Promega) were transformed with each ligated vector, and after overnight incubation positive colonies were chosen. .. From each selected colony the plasmid was purified using the Wizard plus SV miniprep system (Promega).

    Polymerase Chain Reaction:

    Article Title: Shifts from glucose to certain secondary carbon-sources result in activation of the extracytoplasmic function sigma factor ?E in Salmonella enterica serovar Typhimurium
    Article Snippet: .. The 1·4 kb PCR product was purified and ligated into pGEM-T Easy (Promega), and E. coli JM109 competent cells (Promega) were transformed with this, following the manufacturer’s protocols. .. Plasmid minipreps (Wizard Plus Minipreps; Promega) from the ampicillin-resistant transformants were screened by PCR using primers PR48 and PR29 ( ) to determine that the insert was oriented in the same direction as the lacZ gene in order to facilitate cloning into pBAD33.

    Article Title: Bacterial community composition of biofilms in milking machines of two dairy farms assessed by a combination of culture-dependent and –independent methods
    Article Snippet: .. The purified PCR products were used to construct clone libraries with the pGEM-T vector system and were transferred into competent E . coli JM109 cells as specified by the manufacturer (Promega Corp., Madison, WI). .. Inserts of randomly selected clones were amplified using the primer pair M13F and M13R [ ].

    Transformation Assay:

    Article Title: Synbiotic therapy (Bifidobacterium longum/Synergy 1) initiates resolution of inflammation in patients with active ulcerative colitis: a randomised controlled pilot trial
    Article Snippet: .. JM109 competent E coli (Promega) were transformed with each ligated vector, and after overnight incubation positive colonies were chosen. .. From each selected colony the plasmid was purified using the Wizard plus SV miniprep system (Promega).

    Article Title: Identification and Localization of an Arachidonic Acid-Sensitive Potassium Channel in the Cochlea
    Article Snippet: .. The purified c Shal fragment was ligated into the alkaline phosphatase (AP)-treated, Bam HI-cut pcDNA3.1 (+) vector and transformed into JM109-competent cells (Promega, Madison, WI). .. Transformants were screened for ampicillin-resistant recombinant plasmids and extracted using the QIAfilter Plasmid Midi kit (Qiagen) according to the manufacturer's protocol.

    Article Title: pELMO, an optimised in-house cloning vector
    Article Snippet: .. The resulting constructs were transformed in E. coli JM109 competent cells (Promega). .. Clones from each plate were randomly chosen and analysed by colony PCR after 15 h incubation at 37 °C with Np21+/Np6+, PvARNP-D/PvARNP-R and Pvron4intdir/Pvron4intrev primer sets (Table ).

    Article Title: Shifts from glucose to certain secondary carbon-sources result in activation of the extracytoplasmic function sigma factor ?E in Salmonella enterica serovar Typhimurium
    Article Snippet: .. The 1·4 kb PCR product was purified and ligated into pGEM-T Easy (Promega), and E. coli JM109 competent cells (Promega) were transformed with this, following the manufacturer’s protocols. .. Plasmid minipreps (Wizard Plus Minipreps; Promega) from the ampicillin-resistant transformants were screened by PCR using primers PR48 and PR29 ( ) to determine that the insert was oriented in the same direction as the lacZ gene in order to facilitate cloning into pBAD33.

    Article Title: Primers for phylogeny reconstruction in Bignonieae (Bignoniaceae) using herbarium samples 1
    Article Snippet: .. JM109 Competent E. coli cells (Promega Corporation) were used for the heat-shock transformation protocol. .. After incubation, transformant colonies were resuspended in 10 μL of 0.5× TE buffer and boiled for 10 min in a thermocycler.

    Article Title: RAB10 Interacts with the Male Germ Cell-Specific GTPase-Activating Protein during Mammalian Spermiogenesis
    Article Snippet: .. Finally, ligation and transformation was performed using T4 DNA ligase (M0202; New England Biolabs, Ipswich, MA, USA) and JM109 competent cells, respectively (L2001; Promega, Fitchburg, WI, USA) [ , ]. .. Subsequently, NTERA-2 cl.D1 (NT2D1) cells (ATCC, Manassas, VA, USA), a pluripotent human testicular embryonal carcinoma cell line, were transfected with plasmids using Lipofectamine 2000 (Cat No.: 11668; Invitrogen, Carlsbad, CA, USA).

    Plasmid Preparation:

    Article Title: Synbiotic therapy (Bifidobacterium longum/Synergy 1) initiates resolution of inflammation in patients with active ulcerative colitis: a randomised controlled pilot trial
    Article Snippet: .. JM109 competent E coli (Promega) were transformed with each ligated vector, and after overnight incubation positive colonies were chosen. .. From each selected colony the plasmid was purified using the Wizard plus SV miniprep system (Promega).

    Article Title: Identification and Localization of an Arachidonic Acid-Sensitive Potassium Channel in the Cochlea
    Article Snippet: .. The purified c Shal fragment was ligated into the alkaline phosphatase (AP)-treated, Bam HI-cut pcDNA3.1 (+) vector and transformed into JM109-competent cells (Promega, Madison, WI). .. Transformants were screened for ampicillin-resistant recombinant plasmids and extracted using the QIAfilter Plasmid Midi kit (Qiagen) according to the manufacturer's protocol.

    Article Title: Bacterial community composition of biofilms in milking machines of two dairy farms assessed by a combination of culture-dependent and –independent methods
    Article Snippet: .. The purified PCR products were used to construct clone libraries with the pGEM-T vector system and were transferred into competent E . coli JM109 cells as specified by the manufacturer (Promega Corp., Madison, WI). .. Inserts of randomly selected clones were amplified using the primer pair M13F and M13R [ ].

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  • 91
    Promega high efficiency jm109 escherichia coli competent cells
    High Efficiency Jm109 Escherichia Coli Competent Cells, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high efficiency jm109 escherichia coli competent cells/product/Promega
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    high efficiency jm109 escherichia coli competent cells - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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    Promega escherichia coli jm109 high efficiency chemical competent cells
    Escherichia Coli Jm109 High Efficiency Chemical Competent Cells, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli jm109 high efficiency chemical competent cells/product/Promega
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli jm109 high efficiency chemical competent cells - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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