escherichia coli jm109 high efficiency competent cells  (Promega)

 
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    Promega escherichia coli jm109 high efficiency competent cells
    Escherichia Coli Jm109 High Efficiency Competent Cells, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli jm109 high efficiency competent cells/product/Promega
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli jm109 high efficiency competent cells - by Bioz Stars, 2020-01
    91/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Mutualism breakdown in breadfruit domestication
    Article Snippet: .. These were purified using the QiAquick PCR purification kit (Qiagen) and cloned using the pGEM-T vector (Promega, USA) and Escherichia coli JM109 High Efficiency Competent cells (Promega). ..

    Article Title: Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil
    Article Snippet: .. PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, WI). .. Based on the PCR-DGGE analyses, cloned amplicons corresponding in electrophoretic mobility to nematode-specific bands were sequenced (Macrogen, Amsterdam, Netherlands).

    Article Title: Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria
    Article Snippet: Paragraph title: Transformation and cloning of PCR products. ... Amplified products were ligated into the pGEM-T easy vector (Promega), and the ligated vector was transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) by the heat shock method.

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA). .. Cloned amplicons corresponding in electrophoretic mobility to nematode-specific bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Article Title: Identification of msp1 Gene Variants in Populations of Meloidogyne incognita Using PCR-DGGE
    Article Snippet: .. For the sequencing of the different bands of msp1 gene fragments observed at different positions in the DGGE gel, PCR products obtained with the primers msp410f and MImsp596r were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells according to the instructions of the manufacturer (Promega, Madison, WI). .. Based on PCR-DGGE, cloned amplicons corresponding in electrophoretic mobility to different bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: .. Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced. .. The nucleotide sequence of the cloned Carassius auratus CB2 340 bp fragment is available at GenBank (accession number: ).

    Article Title: Identification of Active Denitrifiers in Rice Paddy Soil by DNA- and RNA-Based Analyses
    Article Snippet: The PCR products were ligated into the pGEM-T vector system (Promega, Madison, WI, USA) and transferred to Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer’s instructions. .. The 16S rRNA clones sharing nucleotide sequence homology at more than 99% were grouped into one operational taxonomic unit (OTU) using DOTUR program ver.

    Article Title: Identification and isolation of active N2O reducers in rice paddy soil
    Article Snippet: .. After removing excess primers and dNTP by using a Wizard DNA Cleanup system (Promega, Madison, WI, USA), PCR products were cloned into a pGEM-T Easy vector (Promega) and transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer's instructions. .. DNA inserts from randomly selected clones were amplified by PCR with vector primers T7-1 and SP6, and sequenced as described previously ( ).

    Amplification:

    Article Title: Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil
    Article Snippet: PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, WI). .. Barcoded amplicon pyrosequencing was used to analyze 16S rRNA genes of total J2-associated bacteria.

    Article Title: Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria
    Article Snippet: .. Amplified products were ligated into the pGEM-T easy vector (Promega), and the ligated vector was transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) by the heat shock method. .. Transformation was detected on LB agar plates supplemented with ampicillin/IPTG/X-Gal by screening the blue-white colonies.

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: The fungal ITS fragments were amplified using a nested PCR approach with primer pairs ITS1F / ITS4 and ITS1FGC / ITS2. .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA).

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: The 380 bp amplification product was cloned into pGEM-T-easy vector, using pGEM-T-easy Vector System (Promega, Madison, USA). .. Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced.

    Article Title: Identification of Active Denitrifiers in Rice Paddy Soil by DNA- and RNA-Based Analyses
    Article Snippet: For clone library analysis, DNA and cDNA samples were amplified with the modified primers 27F and 1492R , Cd3aF and R3cd , F1aCu and R3Cu ( ) and nosZ-F-1181 and nosZ-R-1880 ( ) for the bacterial 16S rRNA gene, nirS , nirK and nosZ , respectively. .. The PCR products were ligated into the pGEM-T vector system (Promega, Madison, WI, USA) and transferred to Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer’s instructions.

    Article Title: Identification and isolation of active N2O reducers in rice paddy soil
    Article Snippet: For culture-independent clone library analysis of the microbial community in the heavy fractions from 13SN and 13Su samples, the 16S rRNA gene and nosZ were PCR amplified using primers m-27F and m-1492R ( ) and nosZ-F-1181 and nosZ-R-1880 , respectively. .. After removing excess primers and dNTP by using a Wizard DNA Cleanup system (Promega, Madison, WI, USA), PCR products were cloned into a pGEM-T Easy vector (Promega) and transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer's instructions.

    Synthesized:

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: DNA contaminants were eliminated using TURBO DNA-free kit (Applied Biosystems, Foster City, USA). cDNA was synthesized from total RNA by using Multiscribe RT (Applied Biosystems) and random nonamers. .. Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced.

    Construct:

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: In order to construct the subtype reference plasmids, RNA was isolated from the viral reference culture supernatants with the Boom extraction method and used for RT‐PCR with Superscript‐III One‐Step Platinum Taq (Invitrogen, Carlsbad, CA, USA) and nested PCR with Platinum Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) using isolate‐specific primers (Tables ). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

    Incubation:

    Article Title: Identification of Active Denitrifiers in Rice Paddy Soil by DNA- and RNA-Based Analyses
    Article Snippet: For samples with low concentrations of PCR template (nirK and nosZ amplicons from RNA samples and nirS amplicons from RNA samples extracted from the soil before incubation), a second PCR reaction was performed using a 10-fold diluted product of the first PCR reaction as the template. .. The PCR products were ligated into the pGEM-T vector system (Promega, Madison, WI, USA) and transferred to Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer’s instructions.

    Modification:

    Article Title: Identification of Active Denitrifiers in Rice Paddy Soil by DNA- and RNA-Based Analyses
    Article Snippet: For clone library analysis, DNA and cDNA samples were amplified with the modified primers 27F and 1492R , Cd3aF and R3cd , F1aCu and R3Cu ( ) and nosZ-F-1181 and nosZ-R-1880 ( ) for the bacterial 16S rRNA gene, nirS , nirK and nosZ , respectively. .. The PCR products were ligated into the pGEM-T vector system (Promega, Madison, WI, USA) and transferred to Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer’s instructions.

    Transformation Assay:

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen). .. Plasmids were digested using Eco RI and Buffer H (Roche, Basel, Switzerland) prior to diluting, in order to ensure the repeatability of the dilutions.

    Article Title: Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria
    Article Snippet: .. Amplified products were ligated into the pGEM-T easy vector (Promega), and the ligated vector was transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) by the heat shock method. .. Transformation was detected on LB agar plates supplemented with ampicillin/IPTG/X-Gal by screening the blue-white colonies.

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: .. Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced. .. The nucleotide sequence of the cloned Carassius auratus CB2 340 bp fragment is available at GenBank (accession number: ).

    Article Title: Identification and isolation of active N2O reducers in rice paddy soil
    Article Snippet: .. After removing excess primers and dNTP by using a Wizard DNA Cleanup system (Promega, Madison, WI, USA), PCR products were cloned into a pGEM-T Easy vector (Promega) and transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer's instructions. .. DNA inserts from randomly selected clones were amplified by PCR with vector primers T7-1 and SP6, and sequenced as described previously ( ).

    Cell Culture:

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen). .. Plasmids were digested using Eco RI and Buffer H (Roche, Basel, Switzerland) prior to diluting, in order to ensure the repeatability of the dilutions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: In order to construct the subtype reference plasmids, RNA was isolated from the viral reference culture supernatants with the Boom extraction method and used for RT‐PCR with Superscript‐III One‐Step Platinum Taq (Invitrogen, Carlsbad, CA, USA) and nested PCR with Platinum Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) using isolate‐specific primers (Tables ). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

    Generated:

    Article Title: Mutualism breakdown in breadfruit domestication
    Article Snippet: We generated an experiment-wide clone library using amplicons pooled across the six cultivation sites. .. These were purified using the QiAquick PCR purification kit (Qiagen) and cloned using the pGEM-T vector (Promega, USA) and Escherichia coli JM109 High Efficiency Competent cells (Promega).

    Sequencing:

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: The results were summarized by subtype or CRF and for each plasmid the sequence variants of the corresponding subtype or CRF were extracted. .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

    Article Title: Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil
    Article Snippet: For the DGGE fingerprints of bacterial groups and fungal ITS fragments that showed nematode-specific bands, PCR products were cloned and sequenced to identify the corresponding microbial species by sequence comparison to the GenBank entries. .. PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, WI).

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: The concentration of the purified amplicon samples was measured using a Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA), the samples were pooled and adjusted to equimolar concentrations, concentrated using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA), and finally subjected to 2x250 bp paired-end high-throughput sequencing on an Illumina® MiSeq® platform (Illumina, San Diego, CA, USA). .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA).

    Article Title: Identification of msp1 Gene Variants in Populations of Meloidogyne incognita Using PCR-DGGE
    Article Snippet: .. For the sequencing of the different bands of msp1 gene fragments observed at different positions in the DGGE gel, PCR products obtained with the primers msp410f and MImsp596r were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells according to the instructions of the manufacturer (Promega, Madison, WI). .. Based on PCR-DGGE, cloned amplicons corresponding in electrophoretic mobility to different bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: Paragraph title: Cloning and sequence analysis of goldfish CB2 partial coding sequence ... Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced.

    Article Title: Identification of Active Denitrifiers in Rice Paddy Soil by DNA- and RNA-Based Analyses
    Article Snippet: The PCR products were ligated into the pGEM-T vector system (Promega, Madison, WI, USA) and transferred to Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer’s instructions. .. The 16S rRNA clones sharing nucleotide sequence homology at more than 99% were grouped into one operational taxonomic unit (OTU) using DOTUR program ver.

    Article Title: Identification and isolation of active N2O reducers in rice paddy soil
    Article Snippet: Paragraph title: PCR, cloning and sequencing ... After removing excess primers and dNTP by using a Wizard DNA Cleanup system (Promega, Madison, WI, USA), PCR products were cloned into a pGEM-T Easy vector (Promega) and transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer's instructions.

    Denaturing Gradient Gel Electrophoresis:

    Article Title: Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil
    Article Snippet: For the DGGE fingerprints of bacterial groups and fungal ITS fragments that showed nematode-specific bands, PCR products were cloned and sequenced to identify the corresponding microbial species by sequence comparison to the GenBank entries. .. PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, WI).

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA). .. Cloned amplicons corresponding in electrophoretic mobility to nematode-specific bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Article Title: Identification of msp1 Gene Variants in Populations of Meloidogyne incognita Using PCR-DGGE
    Article Snippet: .. For the sequencing of the different bands of msp1 gene fragments observed at different positions in the DGGE gel, PCR products obtained with the primers msp410f and MImsp596r were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells according to the instructions of the manufacturer (Promega, Madison, WI). .. Based on PCR-DGGE, cloned amplicons corresponding in electrophoretic mobility to different bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Cellular Antioxidant Activity Assay:

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: The 5’ sense primer, corresponding to zebrafish CB2 bases 437–456 (considering position 1 as the first nucleotide of the coding sequence), was as follows: 5’-TTT GCA TCT ACC AGG CTT CC-3’; the 3’ antisense primer, corresponding to zebrafish CB2 bases 797–816, had the following sequence: 5’-CAG GAT TAG AAG GAT CAA AC-3’. .. Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced.

    Isolation:

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen). .. Plasmids were digested using Eco RI and Buffer H (Roche, Basel, Switzerland) prior to diluting, in order to ensure the repeatability of the dilutions.

    Purification:

    Article Title: Mutualism breakdown in breadfruit domestication
    Article Snippet: .. These were purified using the QiAquick PCR purification kit (Qiagen) and cloned using the pGEM-T vector (Promega, USA) and Escherichia coli JM109 High Efficiency Competent cells (Promega). ..

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: The concentration of the purified amplicon samples was measured using a Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA), the samples were pooled and adjusted to equimolar concentrations, concentrated using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA), and finally subjected to 2x250 bp paired-end high-throughput sequencing on an Illumina® MiSeq® platform (Illumina, San Diego, CA, USA). .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA).

    Polymerase Chain Reaction:

    Article Title: Mutualism breakdown in breadfruit domestication
    Article Snippet: .. These were purified using the QiAquick PCR purification kit (Qiagen) and cloned using the pGEM-T vector (Promega, USA) and Escherichia coli JM109 High Efficiency Competent cells (Promega). ..

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

    Article Title: Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil
    Article Snippet: .. PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, WI). .. Based on the PCR-DGGE analyses, cloned amplicons corresponding in electrophoretic mobility to nematode-specific bands were sequenced (Macrogen, Amsterdam, Netherlands).

    Article Title: Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria
    Article Snippet: Paragraph title: Transformation and cloning of PCR products. ... Amplified products were ligated into the pGEM-T easy vector (Promega), and the ligated vector was transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) by the heat shock method.

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA). .. Cloned amplicons corresponding in electrophoretic mobility to nematode-specific bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Article Title: Identification of msp1 Gene Variants in Populations of Meloidogyne incognita Using PCR-DGGE
    Article Snippet: .. For the sequencing of the different bands of msp1 gene fragments observed at different positions in the DGGE gel, PCR products obtained with the primers msp410f and MImsp596r were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells according to the instructions of the manufacturer (Promega, Madison, WI). .. Based on PCR-DGGE, cloned amplicons corresponding in electrophoretic mobility to different bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: PCR was performed for 40 cycles, at 45 °C annealing temperature, using Hot Start AmpliTaq Gold360 polymerase (Applied Biosystems). .. Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced.

    Article Title: Identification of Active Denitrifiers in Rice Paddy Soil by DNA- and RNA-Based Analyses
    Article Snippet: .. The PCR products were ligated into the pGEM-T vector system (Promega, Madison, WI, USA) and transferred to Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer’s instructions. .. The 16S rRNA clones sharing nucleotide sequence homology at more than 99% were grouped into one operational taxonomic unit (OTU) using DOTUR program ver.

    Article Title: Identification and isolation of active N2O reducers in rice paddy soil
    Article Snippet: .. After removing excess primers and dNTP by using a Wizard DNA Cleanup system (Promega, Madison, WI, USA), PCR products were cloned into a pGEM-T Easy vector (Promega) and transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer's instructions. .. DNA inserts from randomly selected clones were amplified by PCR with vector primers T7-1 and SP6, and sequenced as described previously ( ).

    Nested PCR:

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: In order to construct the subtype reference plasmids, RNA was isolated from the viral reference culture supernatants with the Boom extraction method and used for RT‐PCR with Superscript‐III One‐Step Platinum Taq (Invitrogen, Carlsbad, CA, USA) and nested PCR with Platinum Taq DNA Polymerase High Fidelity (Thermo Fisher Scientific, Waltham, MA, USA) using isolate‐specific primers (Tables ). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: The fungal ITS fragments were amplified using a nested PCR approach with primer pairs ITS1F / ITS4 and ITS1FGC / ITS2. .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA).

    Plasmid Preparation:

    Article Title: Mutualism breakdown in breadfruit domestication
    Article Snippet: .. These were purified using the QiAquick PCR purification kit (Qiagen) and cloned using the pGEM-T vector (Promega, USA) and Escherichia coli JM109 High Efficiency Competent cells (Promega). ..

    Article Title: Development of sensitive dd PCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant formsDevelopment of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
    Article Snippet: Amplicons were purified from PCR reactions with Qiaquick PCR Purification kit (Qiagen, Hilden, Germany), A‐tailed with DreamTaq DNA polymerase (Thermo Fisher Scientific), again purified with the Qiaquick PCR purification kit (Qiagen), ligated into vector pGEM‐T Easy (Promega, Madison, WI, USA) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) and again purified with the Qiaquick PCR Purification kit (Qiagen). .. Escherichia coli JM109 High Efficiency Competent Cells (Promega) were used for transformation, colonies were picked and cultured overnight at 37°C in 5 mL LB medium supplemented with ampicillin (40 μg/mL) and plasmids were isolated using the Qiaprep Spin Miniprep Kit (Qiagen).

    Article Title: Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil
    Article Snippet: .. PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, WI). .. Based on the PCR-DGGE analyses, cloned amplicons corresponding in electrophoretic mobility to nematode-specific bands were sequenced (Macrogen, Amsterdam, Netherlands).

    Article Title: Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria
    Article Snippet: .. Amplified products were ligated into the pGEM-T easy vector (Promega), and the ligated vector was transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) by the heat shock method. .. Transformation was detected on LB agar plates supplemented with ampicillin/IPTG/X-Gal by screening the blue-white colonies.

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA). .. Cloned amplicons corresponding in electrophoretic mobility to nematode-specific bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Article Title: Identification of msp1 Gene Variants in Populations of Meloidogyne incognita Using PCR-DGGE
    Article Snippet: .. For the sequencing of the different bands of msp1 gene fragments observed at different positions in the DGGE gel, PCR products obtained with the primers msp410f and MImsp596r were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells according to the instructions of the manufacturer (Promega, Madison, WI). .. Based on PCR-DGGE, cloned amplicons corresponding in electrophoretic mobility to different bands were sequenced (Macrogen, Amsterdam, The Netherlands).

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: The 380 bp amplification product was cloned into pGEM-T-easy vector, using pGEM-T-easy Vector System (Promega, Madison, USA). .. Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced.

    Article Title: Identification of Active Denitrifiers in Rice Paddy Soil by DNA- and RNA-Based Analyses
    Article Snippet: .. The PCR products were ligated into the pGEM-T vector system (Promega, Madison, WI, USA) and transferred to Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer’s instructions. .. The 16S rRNA clones sharing nucleotide sequence homology at more than 99% were grouped into one operational taxonomic unit (OTU) using DOTUR program ver.

    Article Title: Identification and isolation of active N2O reducers in rice paddy soil
    Article Snippet: .. After removing excess primers and dNTP by using a Wizard DNA Cleanup system (Promega, Madison, WI, USA), PCR products were cloned into a pGEM-T Easy vector (Promega) and transformed into Escherichia coli JM109 high-efficiency competent cells (Promega) according to the manufacturer's instructions. .. DNA inserts from randomly selected clones were amplified by PCR with vector primers T7-1 and SP6, and sequenced as described previously ( ).

    Recombinant:

    Article Title: Cannabinoid receptors are widely expressed in goldfish: molecular cloning of a CB2-like receptor and evaluation of CB1 and CB2 mRNA expression profiles in different organs
    Article Snippet: .. Escherichia coli JM109 high efficiency competent cells (Promega) were transformed and recombinant colonies were identified by blue/white color screening and restriction digestion; 6 selected recombinant clones (pGEM-T-easy-CB2 Car ) were sequenced. .. The nucleotide sequence of the cloned Carassius auratus CB2 340 bp fragment is available at GenBank (accession number: ).

    Produced:

    Article Title: Specific Microbial Attachment to Root Knot Nematodes in Suppressive Soil
    Article Snippet: For Alphaproteobacteria and Pseudomonas , PCR products obtained with the primer pair F984GC/R1378 were used; for Bacillus , products produced with the primer pair BacF/R1378 were used; for fungal profiles, products of the primer pair ITS1FGC/ITS2 were used (see Table S1 in the supplemental material). .. PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, WI).

    Concentration Assay:

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: The concentration of the purified amplicon samples was measured using a Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA), the samples were pooled and adjusted to equimolar concentrations, concentrated using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA), and finally subjected to 2x250 bp paired-end high-throughput sequencing on an Illumina® MiSeq® platform (Illumina, San Diego, CA, USA). .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA).

    High Throughput Screening Assay:

    Article Title: Microbiomes associated with infective stages of root-knot and lesion nematodes in soil
    Article Snippet: The concentration of the purified amplicon samples was measured using a Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA), the samples were pooled and adjusted to equimolar concentrations, concentrated using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA), and finally subjected to 2x250 bp paired-end high-throughput sequencing on an Illumina® MiSeq® platform (Illumina, San Diego, CA, USA). .. To identify the bacterial and fungal species corresponding to some of the bands of nematode associated DGGE profiles, PCR products were cloned using the vector pGEM-T and Escherichia coli JM109 high-efficiency competent cells (Promega, Madison, USA).

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    Promega jm109 competent cells
    Jm109 Competent Cells, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega escherichia coli jm109 high efficiency competent cells
    Escherichia Coli Jm109 High Efficiency Competent Cells, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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