escherichia coli de3 novablue cells  (Millipore)


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    Structured Review

    Millipore escherichia coli de3 novablue cells
    Escherichia Coli De3 Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli de3 novablue cells/product/Millipore
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli de3 novablue cells - by Bioz Stars, 2020-02
    83/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Recombinant O. tsutsugamushi outer membrane protein A (OmpA; OTT_1320) was generated using primers 5′- GACGACGACAAGATA TGTTTATGGCAAAGATCTAAACATAGTAAC-3′ and 5′- GAGGAGAAGCCCGGTTA TTTATGTTTCCCATGTATAGCTTGTAAAAACTG-3′ (sequences that are compatible with ligation-independent cloning are italicized) to amplify the region corresponding to amino acids 22 to 93, which is unique to OmpA, as assessed by NCBI BLAST (Basic Local Alignment Search Tool) ( ) searches. .. Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore).

    Amplification:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: The primers harbored ligase-independent cloning (LIC) tails, and the amplicon was annealed with pET46 Ek/LIC (Novagen, EMD Millipore, Billerica, MA). .. Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore).

    Ligation:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Recombinant O. tsutsugamushi outer membrane protein A (OmpA; OTT_1320) was generated using primers 5′- GACGACGACAAGATA TGTTTATGGCAAAGATCTAAACATAGTAAC-3′ and 5′- GAGGAGAAGCCCGGTTA TTTATGTTTCCCATGTATAGCTTGTAAAAACTG-3′ (sequences that are compatible with ligation-independent cloning are italicized) to amplify the region corresponding to amino acids 22 to 93, which is unique to OmpA, as assessed by NCBI BLAST (Basic Local Alignment Search Tool) ( ) searches. .. Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore).

    Ligase Independent Cloning:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: The primers harbored ligase-independent cloning (LIC) tails, and the amplicon was annealed with pET46 Ek/LIC (Novagen, EMD Millipore, Billerica, MA). .. Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore).

    Purification:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore). .. The final recombinant protein, which carried an N-terminal 6×His tag of 1.7 kDa, was purified ( > 95% homogeneity) by immobilized metal affinity chromatography (IMAC) (HisTrap; GE Healthcare Biosciences, Pittsburgh, PA) according to the manufacturer's protocol and concentrated using Amicon Ultra filters (EMD Millipore).

    Acid Assay:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore). .. The protein concentration was determined using the bicinchoninic acid assay.

    Generated:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Recombinant O. tsutsugamushi outer membrane protein A (OmpA; OTT_1320) was generated using primers 5′- GACGACGACAAGATA TGTTTATGGCAAAGATCTAAACATAGTAAC-3′ and 5′- GAGGAGAAGCCCGGTTA TTTATGTTTCCCATGTATAGCTTGTAAAAACTG-3′ (sequences that are compatible with ligation-independent cloning are italicized) to amplify the region corresponding to amino acids 22 to 93, which is unique to OmpA, as assessed by NCBI BLAST (Basic Local Alignment Search Tool) ( ) searches. .. Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore).

    Affinity Chromatography:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore). .. The final recombinant protein, which carried an N-terminal 6×His tag of 1.7 kDa, was purified ( > 95% homogeneity) by immobilized metal affinity chromatography (IMAC) (HisTrap; GE Healthcare Biosciences, Pittsburgh, PA) according to the manufacturer's protocol and concentrated using Amicon Ultra filters (EMD Millipore).

    Expressing:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore). .. Protein was expressed by inoculation of 100 ml Luria-Burtani (LB) broth supplemented with 100 mg liter−1 of ampicillin with a colony from freshly plated E. coli , and the culture was allowed to autoinduce protein expression overnight.

    Protein Concentration:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore). .. The protein concentration was determined using the bicinchoninic acid assay.

    Affinity Purification:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Affinity-purified rabbit polyclonal antiserum targeting Ank9 amino acids 12 to 28 was generated by New England Peptide (Gardner, MA). .. Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore).

    Recombinant:

    Article Title: Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs
    Article Snippet: Recombinant O. tsutsugamushi outer membrane protein A (OmpA; OTT_1320) was generated using primers 5′- GACGACGACAAGATA TGTTTATGGCAAAGATCTAAACATAGTAAC-3′ and 5′- GAGGAGAAGCCCGGTTA TTTATGTTTCCCATGTATAGCTTGTAAAAACTG-3′ (sequences that are compatible with ligation-independent cloning are italicized) to amplify the region corresponding to amino acids 22 to 93, which is unique to OmpA, as assessed by NCBI BLAST (Basic Local Alignment Search Tool) ( ) searches. .. Plasmids were propagated in Escherichia coli DE3 NovaBlue cells (EMD Millipore).

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  • 83
    Millipore escherichia coli de3 novablue cells
    Escherichia Coli De3 Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli de3 novablue cells/product/Millipore
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli de3 novablue cells - by Bioz Stars, 2020-02
    83/100 stars
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