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tiangen biotech co escherichia coli competent cells
Escherichia Coli Competent Cells, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/escherichia coli competent cells/product/tiangen biotech co
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
escherichia coli competent cells - by Bioz Stars, 2020-08
92/100 stars

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Clone Assay:

Article Title: Major histocompatibility complex alleles associated with parasite susceptibility in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18-T vector system (Takara, Dalian, China), and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). ..

Article Title: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
Article Snippet: .. Construction of plasmids containing the cap gene of PCV3 The target fragment was amplified using primers PCV3-F3 and PCV3-B3 and cloned into the pMD-18 T vector (Takara, China), which was transformed into Escherichia coli competent cells according to the manufacturer’s instructions (DH5α, Tiangen, Beijing, China). .. The plasmid containing the PCV3 cap gene (pMD-18 T-cap) was purified using a commercial kit (Tiangen, Beijing, China).

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

RNA Extraction:

Article Title: Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis
Article Snippet: .. The RNA extraction kit was purchased from Shanghai Bocai Biotechnology Company; Quantscript RT kit, pGM-T vector and Escherichia coli competent cells (DH5α) from Tiangen Biotechnology Limited Company; Taq PCR kit from TaKaRa Biotechnology Limited Company; and primer synthesis and DNA sequencing were commissioned by Shanghai Sang’ni Biotechnology Company. ..

Amplification:

Article Title: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
Article Snippet: .. Construction of plasmids containing the cap gene of PCV3 The target fragment was amplified using primers PCV3-F3 and PCV3-B3 and cloned into the pMD-18 T vector (Takara, China), which was transformed into Escherichia coli competent cells according to the manufacturer’s instructions (DH5α, Tiangen, Beijing, China). .. The plasmid containing the PCV3 cap gene (pMD-18 T-cap) was purified using a commercial kit (Tiangen, Beijing, China).

Purification:

Article Title: Major histocompatibility complex alleles associated with parasite susceptibility in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18-T vector system (Takara, Dalian, China), and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). ..

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

DNA Sequencing:

Article Title: Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis
Article Snippet: .. The RNA extraction kit was purchased from Shanghai Bocai Biotechnology Company; Quantscript RT kit, pGM-T vector and Escherichia coli competent cells (DH5α) from Tiangen Biotechnology Limited Company; Taq PCR kit from TaKaRa Biotechnology Limited Company; and primer synthesis and DNA sequencing were commissioned by Shanghai Sang’ni Biotechnology Company. ..

Polymerase Chain Reaction:

Article Title: Major histocompatibility complex alleles associated with parasite susceptibility in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18-T vector system (Takara, Dalian, China), and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). ..

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

Article Title: Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis
Article Snippet: .. The RNA extraction kit was purchased from Shanghai Bocai Biotechnology Company; Quantscript RT kit, pGM-T vector and Escherichia coli competent cells (DH5α) from Tiangen Biotechnology Limited Company; Taq PCR kit from TaKaRa Biotechnology Limited Company; and primer synthesis and DNA sequencing were commissioned by Shanghai Sang’ni Biotechnology Company. ..

Article Title: Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense
Article Snippet: .. The 17-cycle PCR mixture was digested by Dpn I to remove the template before transformed into E. coli competent cells provided by the manufacturer (Tiangen). .. The WT and mutant Bk TauF proteins were purified by Talon affinity column, followed by TEV protease cleavage.

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

Transformation Assay:

Article Title: Major histocompatibility complex alleles associated with parasite susceptibility in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18-T vector system (Takara, Dalian, China), and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). ..

Article Title: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
Article Snippet: .. Construction of plasmids containing the cap gene of PCV3 The target fragment was amplified using primers PCV3-F3 and PCV3-B3 and cloned into the pMD-18 T vector (Takara, China), which was transformed into Escherichia coli competent cells according to the manufacturer’s instructions (DH5α, Tiangen, Beijing, China). .. The plasmid containing the PCV3 cap gene (pMD-18 T-cap) was purified using a commercial kit (Tiangen, Beijing, China).

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

Article Title: Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense
Article Snippet: .. The 17-cycle PCR mixture was digested by Dpn I to remove the template before transformed into E. coli competent cells provided by the manufacturer (Tiangen). .. The WT and mutant Bk TauF proteins were purified by Talon affinity column, followed by TEV protease cleavage.

Article Title: Identification of UAP1L1 as tumor promotor in gastric cancer through regulation of CDK6
Article Snippet: .. Target sequences for UAP1L1 (5’-GCCCTTCTTACTGCAAACCAT-3’) and CDK6 (5’-AAGGATATGATGTTTCAGCTT-3’; 5’-TGGCTGCATATTTGCAGAAAT-3’; 5’-GCCCAACCAATTGAGAAGTTT-3’) were inserted into in BR-V-108 vector and transformed into E. coli competent cells (Tiangen, Beijing, China). .. EndoFree maxi plasmid kit (Tiangen, Beijing, China) was used for plasmid extraction according to the manufacturer’s instruction.

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

Plasmid Preparation:

Article Title: Major histocompatibility complex alleles associated with parasite susceptibility in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18-T vector system (Takara, Dalian, China), and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). ..

Article Title: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
Article Snippet: .. Construction of plasmids containing the cap gene of PCV3 The target fragment was amplified using primers PCV3-F3 and PCV3-B3 and cloned into the pMD-18 T vector (Takara, China), which was transformed into Escherichia coli competent cells according to the manufacturer’s instructions (DH5α, Tiangen, Beijing, China). .. The plasmid containing the PCV3 cap gene (pMD-18 T-cap) was purified using a commercial kit (Tiangen, Beijing, China).

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

Article Title: Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis
Article Snippet: .. The RNA extraction kit was purchased from Shanghai Bocai Biotechnology Company; Quantscript RT kit, pGM-T vector and Escherichia coli competent cells (DH5α) from Tiangen Biotechnology Limited Company; Taq PCR kit from TaKaRa Biotechnology Limited Company; and primer synthesis and DNA sequencing were commissioned by Shanghai Sang’ni Biotechnology Company. ..

Article Title: Identification of UAP1L1 as tumor promotor in gastric cancer through regulation of CDK6
Article Snippet: .. Target sequences for UAP1L1 (5’-GCCCTTCTTACTGCAAACCAT-3’) and CDK6 (5’-AAGGATATGATGTTTCAGCTT-3’; 5’-TGGCTGCATATTTGCAGAAAT-3’; 5’-GCCCAACCAATTGAGAAGTTT-3’) were inserted into in BR-V-108 vector and transformed into E. coli competent cells (Tiangen, Beijing, China). .. EndoFree maxi plasmid kit (Tiangen, Beijing, China) was used for plasmid extraction according to the manufacturer’s instruction.

Article Title: No evidence for MHC‐based mate choice in wild giant pandas. No evidence for MHC‐based mate choice in wild giant pandas
Article Snippet: .. Purified PCR fragments were cloned using the pMD18‐T vector system (Takara, Dalian, China) and then transformed into Escherichia coli competent cells (Tiangen, Beijing, China). .. At least six positive clones per individual were selected at random for Sanger sequencing.

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