escherichia coli competent cells  (Promega)

 
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    Name:
    Single Step KRX Competent Cells
    Description:
    Ideal E coli cells for recombinant protein expression offering efficient transformation and regulated protein expression
    Catalog Number:
    l3002
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Cloning DNA Markers Bacterial Strains and Competent Cells
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    Structured Review

    Promega escherichia coli competent cells
    Ideal E coli cells for recombinant protein expression offering efficient transformation and regulated protein expression
    https://www.bioz.com/result/escherichia coli competent cells/product/Promega
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli competent cells - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Cannabidiol enhances morphine antinociception, diminishes NMDA-mediated seizures and reduces stroke damage via the sigma 1 receptor
    Article Snippet: .. The vector was introduced into E. coli BL21 (KRX #L3002, Promega, Madrid, Spain), and clones were selected on solid medium containing ampicillin. .. After 3 h of induction at room temperature (1 mM IPTG and 0.1% Rhamnose), the cells were collected by centrifugation, and the pellets were maintained at − 80 °C.

    Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
    Article Snippet: .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions. .. Approximately 50 clones were re-amplified for the 28S rRNA gene fragments and digested using the restriction enzyme Taq I (Promega, cutting site 5′T/CGA3′) to perform a screening analysis using RFLPs (Restriction Fragment Length Polymorphisms).

    Article Title: Targeting DXP synthase in human pathogens: enzyme inhibition and antimicrobial activity of butylacetylphosphonate
    Article Snippet: .. Cloning, overproduction, and purification of Yersinia pestis, and Salmonella enterica serovar Typhi DXP synthase The Y. pestis and S. enterica serovar Typhi DXP synthase genes were cloned into the pMCSG28 vector using ligation independent cloning (LIC) methods as previously described, ( , ) and the resulting plasmids were transformed into Single Step KRX competent cells (Promega). .. Cells harboring the overexpression plasmid were grown in TB broth containing 100 μg/mL ampicillin, 34 μg/mL chloramphenicol, and 0.1% L(+)arabinose.

    Article Title: Fenfluramine diminishes NMDA receptor-mediated seizures via its mixed activity at serotonin 5HT2A and type 1 sigma receptors
    Article Snippet: .. The vector was introduced into E. coli BL21 (KRX #L3002, Promega, Madrid, Spain), and clones were selected on solid medium containing ampicillin. .. After 3 h induction at room temperature (1 mM IPTG and 0.1% Rhamnose), the cells were collected by centrifugation, and the pellets were maintained at –80° C. The purification of GST fusion proteins was done under native conditions on GStrap FF columns (GE#17-5130-01, Healthcare, Barcelona, Spain) and when necessary the fusion proteins retained were cleaved on the column with ProTEV protease (Promega, #V605A) and further purification was achieved by high-resolution ion exchange (Enrich Q, BioRad #780-0001) or electroelution of the corresponding gel band (GE 200, Hoefer Scientific Instruments, San Francisco, CA, USA).

    Article Title: Ligands Exert Biased Activity to Regulate Sigma 1 Receptor Interactions With Cationic TRPA1, TRPV1, and TRPM8 Channels
    Article Snippet: .. The vector was introduced into the Escherichia coli BL21 (KRX #L3002, Promega), and clones were selected on solid medium containing ampicillin. .. After 3 h of induction at room temperature (RT), in the presence of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and 0.1% Rhamnose, the cells were collected by centrifugation and maintained at −80°C.

    Article Title: A Miniaturized Technique for Assessing Protein Thermodynamics and Function Using Fast Determination of Quantitative Cysteine Reactivity
    Article Snippet: .. All sequence-verified cloned variants were transformed into E. coli KRX strain (Promega L3002) and stored as glycerol stocks at -80°C. .. Starter cultures from glycerol stocks were diluted 1:500 into auto-induction media ZYM-5052 supplemented with 0.04% L -Rhamnose for delayed induction of the KRX T7 expression system.

    Ligation:

    Article Title: Targeting DXP synthase in human pathogens: enzyme inhibition and antimicrobial activity of butylacetylphosphonate
    Article Snippet: .. Cloning, overproduction, and purification of Yersinia pestis, and Salmonella enterica serovar Typhi DXP synthase The Y. pestis and S. enterica serovar Typhi DXP synthase genes were cloned into the pMCSG28 vector using ligation independent cloning (LIC) methods as previously described, ( , ) and the resulting plasmids were transformed into Single Step KRX competent cells (Promega). .. Cells harboring the overexpression plasmid were grown in TB broth containing 100 μg/mL ampicillin, 34 μg/mL chloramphenicol, and 0.1% L(+)arabinose.

    Purification:

    Article Title: A gatekeeper helix determines the substrate specificity of Sjögren–Larsson Syndrome enzyme fatty aldehyde dehydrogenase
    Article Snippet: .. Expression and purification pPR-IBA2 plasmids containing either wild-type or mutated versions of FALDH were transformed into Single Step (KRX) Competent Cells (Promega). .. FALDH activity was measured at different points of the purification using the fluorescence labelled fatty aldehyde pyrenedecanal (Rambius, Lund, Sweden) as described in .

    Article Title: Legionella pneumophila LidA Affects Nucleotide Binding and Activity of the Host GTPase Rab1
    Article Snippet: .. Recombinant HaloTag-Lem3 was produced in the Single Step (KRX) competent E. coli strain (Promega), and Lem3 was purified using the HaloTag protein purification system according to the manufacturer's instructions. .. Briefly, E. coli cells producing HaloTag-Lem3 were harvested and resuspended in protein purification buffer (50 mM HEPES, 150 mM NaCl, pH 7.5) followed by lysis using a Microfluidics M-110P microfluidizer.

    Article Title: Targeting DXP synthase in human pathogens: enzyme inhibition and antimicrobial activity of butylacetylphosphonate
    Article Snippet: .. Cloning, overproduction, and purification of Yersinia pestis, and Salmonella enterica serovar Typhi DXP synthase The Y. pestis and S. enterica serovar Typhi DXP synthase genes were cloned into the pMCSG28 vector using ligation independent cloning (LIC) methods as previously described, ( , ) and the resulting plasmids were transformed into Single Step KRX competent cells (Promega). .. Cells harboring the overexpression plasmid were grown in TB broth containing 100 μg/mL ampicillin, 34 μg/mL chloramphenicol, and 0.1% L(+)arabinose.

    Produced:

    Article Title: Legionella pneumophila LidA Affects Nucleotide Binding and Activity of the Host GTPase Rab1
    Article Snippet: .. Recombinant HaloTag-Lem3 was produced in the Single Step (KRX) competent E. coli strain (Promega), and Lem3 was purified using the HaloTag protein purification system according to the manufacturer's instructions. .. Briefly, E. coli cells producing HaloTag-Lem3 were harvested and resuspended in protein purification buffer (50 mM HEPES, 150 mM NaCl, pH 7.5) followed by lysis using a Microfluidics M-110P microfluidizer.

    Electroporation Bacterial Transformation:

    Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
    Article Snippet: .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions. .. Approximately 50 clones were re-amplified for the 28S rRNA gene fragments and digested using the restriction enzyme Taq I (Promega, cutting site 5′T/CGA3′) to perform a screening analysis using RFLPs (Restriction Fragment Length Polymorphisms).

    Polymerase Chain Reaction:

    Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
    Article Snippet: .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions. .. Approximately 50 clones were re-amplified for the 28S rRNA gene fragments and digested using the restriction enzyme Taq I (Promega, cutting site 5′T/CGA3′) to perform a screening analysis using RFLPs (Restriction Fragment Length Polymorphisms).

    Expressing:

    Article Title: A gatekeeper helix determines the substrate specificity of Sjögren–Larsson Syndrome enzyme fatty aldehyde dehydrogenase
    Article Snippet: .. Expression and purification pPR-IBA2 plasmids containing either wild-type or mutated versions of FALDH were transformed into Single Step (KRX) Competent Cells (Promega). .. FALDH activity was measured at different points of the purification using the fluorescence labelled fatty aldehyde pyrenedecanal (Rambius, Lund, Sweden) as described in .

    Sequencing:

    Article Title: A Miniaturized Technique for Assessing Protein Thermodynamics and Function Using Fast Determination of Quantitative Cysteine Reactivity
    Article Snippet: .. All sequence-verified cloned variants were transformed into E. coli KRX strain (Promega L3002) and stored as glycerol stocks at -80°C. .. Starter cultures from glycerol stocks were diluted 1:500 into auto-induction media ZYM-5052 supplemented with 0.04% L -Rhamnose for delayed induction of the KRX T7 expression system.

    Transformation Assay:

    Article Title: A gatekeeper helix determines the substrate specificity of Sjögren–Larsson Syndrome enzyme fatty aldehyde dehydrogenase
    Article Snippet: .. Expression and purification pPR-IBA2 plasmids containing either wild-type or mutated versions of FALDH were transformed into Single Step (KRX) Competent Cells (Promega). .. FALDH activity was measured at different points of the purification using the fluorescence labelled fatty aldehyde pyrenedecanal (Rambius, Lund, Sweden) as described in .

    Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
    Article Snippet: .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions. .. Approximately 50 clones were re-amplified for the 28S rRNA gene fragments and digested using the restriction enzyme Taq I (Promega, cutting site 5′T/CGA3′) to perform a screening analysis using RFLPs (Restriction Fragment Length Polymorphisms).

    Article Title: Targeting DXP synthase in human pathogens: enzyme inhibition and antimicrobial activity of butylacetylphosphonate
    Article Snippet: .. Cloning, overproduction, and purification of Yersinia pestis, and Salmonella enterica serovar Typhi DXP synthase The Y. pestis and S. enterica serovar Typhi DXP synthase genes were cloned into the pMCSG28 vector using ligation independent cloning (LIC) methods as previously described, ( , ) and the resulting plasmids were transformed into Single Step KRX competent cells (Promega). .. Cells harboring the overexpression plasmid were grown in TB broth containing 100 μg/mL ampicillin, 34 μg/mL chloramphenicol, and 0.1% L(+)arabinose.

    Article Title: A Miniaturized Technique for Assessing Protein Thermodynamics and Function Using Fast Determination of Quantitative Cysteine Reactivity
    Article Snippet: .. All sequence-verified cloned variants were transformed into E. coli KRX strain (Promega L3002) and stored as glycerol stocks at -80°C. .. Starter cultures from glycerol stocks were diluted 1:500 into auto-induction media ZYM-5052 supplemented with 0.04% L -Rhamnose for delayed induction of the KRX T7 expression system.

    Recombinant:

    Article Title: Legionella pneumophila LidA Affects Nucleotide Binding and Activity of the Host GTPase Rab1
    Article Snippet: .. Recombinant HaloTag-Lem3 was produced in the Single Step (KRX) competent E. coli strain (Promega), and Lem3 was purified using the HaloTag protein purification system according to the manufacturer's instructions. .. Briefly, E. coli cells producing HaloTag-Lem3 were harvested and resuspended in protein purification buffer (50 mM HEPES, 150 mM NaCl, pH 7.5) followed by lysis using a Microfluidics M-110P microfluidizer.

    Protein Purification:

    Article Title: Legionella pneumophila LidA Affects Nucleotide Binding and Activity of the Host GTPase Rab1
    Article Snippet: .. Recombinant HaloTag-Lem3 was produced in the Single Step (KRX) competent E. coli strain (Promega), and Lem3 was purified using the HaloTag protein purification system according to the manufacturer's instructions. .. Briefly, E. coli cells producing HaloTag-Lem3 were harvested and resuspended in protein purification buffer (50 mM HEPES, 150 mM NaCl, pH 7.5) followed by lysis using a Microfluidics M-110P microfluidizer.

    Plasmid Preparation:

    Article Title: Cannabidiol enhances morphine antinociception, diminishes NMDA-mediated seizures and reduces stroke damage via the sigma 1 receptor
    Article Snippet: .. The vector was introduced into E. coli BL21 (KRX #L3002, Promega, Madrid, Spain), and clones were selected on solid medium containing ampicillin. .. After 3 h of induction at room temperature (1 mM IPTG and 0.1% Rhamnose), the cells were collected by centrifugation, and the pellets were maintained at − 80 °C.

    Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
    Article Snippet: .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions. .. Approximately 50 clones were re-amplified for the 28S rRNA gene fragments and digested using the restriction enzyme Taq I (Promega, cutting site 5′T/CGA3′) to perform a screening analysis using RFLPs (Restriction Fragment Length Polymorphisms).

    Article Title: Targeting DXP synthase in human pathogens: enzyme inhibition and antimicrobial activity of butylacetylphosphonate
    Article Snippet: .. Cloning, overproduction, and purification of Yersinia pestis, and Salmonella enterica serovar Typhi DXP synthase The Y. pestis and S. enterica serovar Typhi DXP synthase genes were cloned into the pMCSG28 vector using ligation independent cloning (LIC) methods as previously described, ( , ) and the resulting plasmids were transformed into Single Step KRX competent cells (Promega). .. Cells harboring the overexpression plasmid were grown in TB broth containing 100 μg/mL ampicillin, 34 μg/mL chloramphenicol, and 0.1% L(+)arabinose.

    Article Title: Fenfluramine diminishes NMDA receptor-mediated seizures via its mixed activity at serotonin 5HT2A and type 1 sigma receptors
    Article Snippet: .. The vector was introduced into E. coli BL21 (KRX #L3002, Promega, Madrid, Spain), and clones were selected on solid medium containing ampicillin. .. After 3 h induction at room temperature (1 mM IPTG and 0.1% Rhamnose), the cells were collected by centrifugation, and the pellets were maintained at –80° C. The purification of GST fusion proteins was done under native conditions on GStrap FF columns (GE#17-5130-01, Healthcare, Barcelona, Spain) and when necessary the fusion proteins retained were cleaved on the column with ProTEV protease (Promega, #V605A) and further purification was achieved by high-resolution ion exchange (Enrich Q, BioRad #780-0001) or electroelution of the corresponding gel band (GE 200, Hoefer Scientific Instruments, San Francisco, CA, USA).

    Article Title: Ligands Exert Biased Activity to Regulate Sigma 1 Receptor Interactions With Cationic TRPA1, TRPV1, and TRPM8 Channels
    Article Snippet: .. The vector was introduced into the Escherichia coli BL21 (KRX #L3002, Promega), and clones were selected on solid medium containing ampicillin. .. After 3 h of induction at room temperature (RT), in the presence of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and 0.1% Rhamnose, the cells were collected by centrifugation and maintained at −80°C.

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  • 92
    Promega competent escherichia coli cells
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