escherichia coli bacterial strain novablue  (Millipore)


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    Name:
    Escherichia coli
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    Catalog Number:
    ec9637
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    Structured Review

    Millipore escherichia coli bacterial strain novablue

    https://www.bioz.com/result/escherichia coli bacterial strain novablue/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bacterial strain novablue - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Transgenic Hybrid Poplar for Sustainable and Scalable Production of the Commodity/Specialty Chemical, 2-Phenylethanol
    Article Snippet: .. Heterologous Expression in E. coli RhPAAS and PAR1 genes were cloned in tandem into a pETDuet-1 expression vector (Novagen) as follows. .. PAR1 cDNA was first PCR amplified using primers PAR1-Nde I-DUET-FOR and PAR1-Xho I-DUET-REV ( ) to include Nde I and Xho I restriction enzyme sites at the 5′- and 3′-ends, respectively, then cloned into a pCR4-TOPO vector and excised and then cloned into the MCS2 region of the pETDuet-1 vector.

    Positive Control:

    Article Title: The antimicrobial molecule trappin-2/elafin has anti-parasitic properties and is protective in vivo in a murine model of cerebral malaria
    Article Snippet: .. As a positive control for the ELISA, the supernatant from MNs stimulated with 0.2 μg/ml of LPS (Escherichia coli 0127:B8, from Sigma) was used for T-2 assessment. .. Parasite and T-2 Immunolocalisation assays After preparation of PVM-enclosed merozoite structures (merozoites “bags”) from which the egress of malaria parasites was checked every 1–2 hrs, they were incubated for 2 h with 50 μg/ml of recombinant human T-2 (Sigma).

    Cell Culture:

    Article Title: Control of siRNA expression using the Cre-loxP recombination system
    Article Snippet: .. For residual E.coli lysate, pTriEx-3 Hygro empty vector was introduced into E.coli strain RosettaBlue(DE3)pLacI (Novagen) and cultured in LB medium that contained 1% glucose, 34 µg/ml chloramphenicol, 12.5 µg/ml tetracycline and 100 µg/ml ampicillin at 37°C until absorbance at 600 nm reached 0.7. .. After addition of IPTG to a final concentration of 1 mM, E.coli were harvested and treated in the same way as that of pTriEx-3 Hygro-TAT-NLS-Cre or pTriEx-3 Hygro-TAT-NLS-EGFP.

    Purification:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The antimicrobial molecule trappin-2/elafin has anti-parasitic properties and is protective in vivo in a murine model of cerebral malaria
    Article Snippet: .. As a positive control for the ELISA, the supernatant from MNs stimulated with 0.2 μg/ml of LPS (Escherichia coli 0127:B8, from Sigma) was used for T-2 assessment. .. Parasite and T-2 Immunolocalisation assays After preparation of PVM-enclosed merozoite structures (merozoites “bags”) from which the egress of malaria parasites was checked every 1–2 hrs, they were incubated for 2 h with 50 μg/ml of recombinant human T-2 (Sigma).

    Incubation:

    Article Title: U-Bang-Haequi Tang: A Herbal Prescription that Prevents Acute Inflammation through Inhibition of NF- κB-Mediated Inducible Nitric Oxide Synthase
    Article Snippet: .. Raw264.7 cells were incubated with 1 μ g/mL LPS (Escherichia coli 026:B6; Sigma, St. Louis, MO, USA). .. The cells were incubated without 10% fetal bovine serum for 24 h and then stimulated to LPS or LPS + UBT for the indicated time periods.

    Expressing:

    Article Title: Transgenic Hybrid Poplar for Sustainable and Scalable Production of the Commodity/Specialty Chemical, 2-Phenylethanol
    Article Snippet: .. Heterologous Expression in E. coli RhPAAS and PAR1 genes were cloned in tandem into a pETDuet-1 expression vector (Novagen) as follows. .. PAR1 cDNA was first PCR amplified using primers PAR1-Nde I-DUET-FOR and PAR1-Xho I-DUET-REV ( ) to include Nde I and Xho I restriction enzyme sites at the 5′- and 3′-ends, respectively, then cloned into a pCR4-TOPO vector and excised and then cloned into the MCS2 region of the pETDuet-1 vector.

    Article Title: Comparison of the functional properties of trimeric and monomeric CaiT of Escherichia coli
    Article Snippet: .. E. coli JW0039 (F− ΔcaiT753::kan Δ (araD-araB )567 ΔlacZ4787 (::rrnB-3) λ − rph-1 Δ (rhaD-rhaB )568 hsdR514 ) or E. coli BL21(DE3) pLysS (F− ompT hasdSB (rB− mB− ) gal dcm (DE3) pLysS (camR )) (Novagen) harboring given plasmids was used for expression of the caiT gene of E. coli and biochemical assays. .. For the pulse-chase experiments, E. coli WG170 (F− trp lacZ rpsL thi Δ (putPA )101 proP219 ) was used.

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: .. For construction of E.coli expression plasmid pKN203 a Nde I– Bam HI full-length hDBR1 cDNA fragment from pKN201 was subcloned into Nde I and Bam HI digested pET28a (Novagen). .. The yeast expression plasmids pKN205 and pKN206 were constructed by inserting an Eco RI– Bam HI full-length hDBR1 cDNA fragment from pKN201 into S.cerevisiae expression vectors pRS314GU ( ) and pRS316GU ( ) digested with Eco RI and Bam HI, respectively.

    Lysis:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Plasmid Preparation:

    Article Title: Transgenic Hybrid Poplar for Sustainable and Scalable Production of the Commodity/Specialty Chemical, 2-Phenylethanol
    Article Snippet: .. Heterologous Expression in E. coli RhPAAS and PAR1 genes were cloned in tandem into a pETDuet-1 expression vector (Novagen) as follows. .. PAR1 cDNA was first PCR amplified using primers PAR1-Nde I-DUET-FOR and PAR1-Xho I-DUET-REV ( ) to include Nde I and Xho I restriction enzyme sites at the 5′- and 3′-ends, respectively, then cloned into a pCR4-TOPO vector and excised and then cloned into the MCS2 region of the pETDuet-1 vector.

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: .. For construction of E.coli expression plasmid pKN203 a Nde I– Bam HI full-length hDBR1 cDNA fragment from pKN201 was subcloned into Nde I and Bam HI digested pET28a (Novagen). .. The yeast expression plasmids pKN205 and pKN206 were constructed by inserting an Eco RI– Bam HI full-length hDBR1 cDNA fragment from pKN201 into S.cerevisiae expression vectors pRS314GU ( ) and pRS316GU ( ) digested with Eco RI and Bam HI, respectively.

    Article Title: Control of siRNA expression using the Cre-loxP recombination system
    Article Snippet: .. For residual E.coli lysate, pTriEx-3 Hygro empty vector was introduced into E.coli strain RosettaBlue(DE3)pLacI (Novagen) and cultured in LB medium that contained 1% glucose, 34 µg/ml chloramphenicol, 12.5 µg/ml tetracycline and 100 µg/ml ampicillin at 37°C until absorbance at 600 nm reached 0.7. .. After addition of IPTG to a final concentration of 1 mM, E.coli were harvested and treated in the same way as that of pTriEx-3 Hygro-TAT-NLS-Cre or pTriEx-3 Hygro-TAT-NLS-EGFP.

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