Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: Strains and plasmids used in this study.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques:

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: MG1655 was challenged with sufficient octanoic acid to inhibit growth by 23% (10 mM) in MOPS minimal media at pH 7.0, 37°C, 150 rpm. This diagram shows central metabolism and highlights the regulatory effect of regulators with significantly perturbed activity during C8 challenge, as identified by network component analysis. Dashed lines indicate regulatory connections that were proposed in our previous analysis . Mechanisms for changes in transcription factor activity are discussed in the text.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques: Activity Assay

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: E. coli MG1655 pJTD1 was grown to midlog in minimal media at pH(HCl). Values are the average of 4 biological replicates, with error bars indicating the standard deviation.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: Measurements of the intracellular pH of E. coli MG1655 pJTD1 during C8 challenge while grown in the presence of supplemental arginine and glutamate. The cells were incubated for 3°C in MOPS media to allow utilization of the amino acid-dependent acid resistance systems. All concentrations are 10 mM.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques: Incubation

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: GABA measurements of MG1655 during log phase growth in MOPS with 2% dextrose at 37°C, 150 rpm. All concentrations are 10 mM. GABA: γ-amino butyric acid.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques:

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: a: Membrane lipid profile of MG1655 and strains with altered cfa expression. Strains were incubated with 0–30 mM C8, pH = 7.0. Inset: specific growth rate in the log phase of E. coli with varying cfa expression. C16:0- palmitic acid, C16:1- palmitoleic acid, C17cyc- cyclopropane C17:0, C18:1- vaccenic acid, C18:0- stearic acid, C19cyc- cyclopropane C19:0. The complete lipid profiles are shown in of the Supporting Information. Membrane properties are calculated from a to obtain: b: saturated:unsaturated lipid ratio and c: average lipid length.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques: Expressing, Incubation

Journal: International Journal of Microbiology

Article Title: Evaluations of the Effects of Extremely Low-Frequency Electromagnetic Fields on Growth and Antibiotic Susceptibility of Escherichia coli and Pseudomonas aeruginosa

doi: 10.1155/2012/587293

Figure Lengend Snippet: MIC values ( μ g/mL) for E. coli and P. aeruginosa exposed or not exposed to ELF-EMF (sinusoidal wave; 2 mT; 50 Hz).

Article Snippet: On the contrary, it has been demonstrated that short-term exposure (20–120 min) to an ELF-EMF with a sinusoidal waveform of amplitude ranging from 0.1 to 1 mT and frequency of 50 Hz affected both cell viability and morphology of cultured E. coli ATCC 700926 [ ].

Techniques:

Journal: International Journal of Microbiology

Article Title: Evaluations of the Effects of Extremely Low-Frequency Electromagnetic Fields on Growth and Antibiotic Susceptibility of Escherichia coli and Pseudomonas aeruginosa

doi: 10.1155/2012/587293

Figure Lengend Snippet: Effect of ELF-EMF (sinusoidal wave; 2 mT; 50 Hz) on growth rate of E. coli (a) and P. aeruginosa (b). ELF-EMF: ELF-EMF exposed groups; Ctr: control groups. Data represent means ± SEM from 3 different experiments.

Article Snippet: On the contrary, it has been demonstrated that short-term exposure (20–120 min) to an ELF-EMF with a sinusoidal waveform of amplitude ranging from 0.1 to 1 mT and frequency of 50 Hz affected both cell viability and morphology of cultured E. coli ATCC 700926 [ ].

Techniques:

Journal: International Journal of Microbiology

Article Title: Evaluations of the Effects of Extremely Low-Frequency Electromagnetic Fields on Growth and Antibiotic Susceptibility of Escherichia coli and Pseudomonas aeruginosa

doi: 10.1155/2012/587293

Figure Lengend Snippet: ELF-EMF (sinusoidal wave; 2 mT; 50 Hz) influenced the growth rate of E. coli (a) and P. aeruginosa (b) when incubated in the presence of subinhibitory concentration of kanamycin and amikacin, respectively. ELF-EMF-KAN: ELF-EMF exposed groups incubated in the presence of 1 μ g/mL kanamycin; Ctr-KAN: control groups incubated in the presence of 1 μ g/mL kanamycin; ELF-EMF-AMK: ELF-EMF exposed groups incubated in the presence of 0.5 μ g/mL amikacin; Ctr-AMK: control groups incubated in the presence of 0.5 μ g/mL amikacin. Data represent means ± SEM from 3 different experiments. * P < 0.05 (one-way ANOVA followed by Tukey's test).

Article Snippet: On the contrary, it has been demonstrated that short-term exposure (20–120 min) to an ELF-EMF with a sinusoidal waveform of amplitude ranging from 0.1 to 1 mT and frequency of 50 Hz affected both cell viability and morphology of cultured E. coli ATCC 700926 [ ].

Techniques: Incubation, Concentration Assay