erythrosin er isothiocyanate  (Thermo Fisher)


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    Name:
    Molecular Probes Handbook A Guide to Fluorescent Probes and Labeling Technologies
    Description:
    The Molecular Probes Handbook a guide to fluorescent probes and labeling technologies is the most complete fluorescent labeling and detection reference available Use this extensively updated print version of the Molecular Probes Handbook to help you get the best experimental results for your fluorescent labeling and detection experiments • Comprehensive updates over 3000 technology solutions represented• Extensive scientific references hundreds of new primary literature references to product applications • Reorganized content fresh design for easier access to data structures and information • New technical notes and product highlights get information about the applications you re interested inIn addition to the hardcover version a softcover version is available We also offer an up to date searchable web based version of the Handbook This valuable eleventh edition reference is an essential tool for both cell and molecular biology researchers
    Catalog Number:
    H37126
    Price:
    None
    Category:
    Software and Digital Storage
    Applications:
    Cell Analysis|Cellular Imaging|Labeling Chemistry|Flow Cytometry|Cell Tracing & Tracking|Cell Structure|Cell Viability, Proliferation & Function
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    Structured Review

    Thermo Fisher erythrosin er isothiocyanate
    The Molecular Probes Handbook a guide to fluorescent probes and labeling technologies is the most complete fluorescent labeling and detection reference available Use this extensively updated print version of the Molecular Probes Handbook to help you get the best experimental results for your fluorescent labeling and detection experiments • Comprehensive updates over 3000 technology solutions represented• Extensive scientific references hundreds of new primary literature references to product applications • Reorganized content fresh design for easier access to data structures and information • New technical notes and product highlights get information about the applications you re interested inIn addition to the hardcover version a softcover version is available We also offer an up to date searchable web based version of the Handbook This valuable eleventh edition reference is an essential tool for both cell and molecular biology researchers
    https://www.bioz.com/result/erythrosin er isothiocyanate/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    erythrosin er isothiocyanate - by Bioz Stars, 2021-05
    97/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Two-dimensional confocal images of organization, density, and gating of focal Ca2+ release sites in rat cardiac myocytes
    Article Snippet: .. In some experiments the measurements of Ca2+ -induced fluo-3 fluorescence was followed by examination of surface and t-tubular membrane structures in the same myocyte, labeled with the fluorescent indicator dye di-2-ANEPEQ (Molecular Probes). .. Effective staining with ANEPEQ required 1–3 min of superfusion using 5-μM concentration of the dye.

    Article Title: Astaxanthin Restrains Nitrative-Oxidative Peroxidation in Mitochondrial-Mimetic Liposomes: A Pre-Apoptosis Model
    Article Snippet: .. Nitric Oxide Concentration The NO• concentration was measured by the fluorescence intensity (λexc = 495 nm; λem = 515 nm) of the probe 4-amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM; Molecular Probes® , Fisher Scientific, Pittsburgh, PA, USA). .. The manufacturers state that the fluorescence quantum yield (ϕF ) of DAF-FM is c.a.

    Labeling:

    Article Title: Two-dimensional confocal images of organization, density, and gating of focal Ca2+ release sites in rat cardiac myocytes
    Article Snippet: .. In some experiments the measurements of Ca2+ -induced fluo-3 fluorescence was followed by examination of surface and t-tubular membrane structures in the same myocyte, labeled with the fluorescent indicator dye di-2-ANEPEQ (Molecular Probes). .. Effective staining with ANEPEQ required 1–3 min of superfusion using 5-μM concentration of the dye.

    Article Title: Interaction of the Spo20 Membrane-Sensor Motif with Phosphatidic Acid and Other Anionic Lipids, and Influence of the Membrane Environment
    Article Snippet: For details about the construction of bioprobes based on artificial coiled-coil (ACC1 and ACC2) and their exact nucleotide and amino-acid sequence see . .. NBD labeling of Spo20 NBD labeling of Spo20-GCC and its mutant swapSpo20-GCC, both displaying two endogenous Cys residues (Cys54 and Cys82) flanking the spo20 sensor region, was performed as described for the N-terminal region of GMAP210 , , , using a 10-fold molar excess of N,N′-dimethyl-N-(iodoacetyl)-N′-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine (IANBD amide, Molecular Probes) in dimethylformamide (DMF volume < 5%). ..

    Article Title: Flow Sorting of Marine Bacterioplankton after Fluorescence In Situ Hybridization
    Article Snippet: .. First, samples were hybridized with probe BET42a, and signal amplification was carried out by using tyramides labeled with Alexa 546 (Molecular Probes, Eugene, Oreg.). .. Next, probe-delivered peroxidase was quenched by incubation with 0.01 M HCl for 10 min and rinsing with ddH2 O.

    Incubation:

    Article Title: Disruption of IFN-γ-Mediated Antiviral Activity in Neurons: The Role of Cannabinoids
    Article Snippet: Greiss reagent was added to triplicate samples, and the product was read with a Bio-Rad Microplate model 550 Reader at 540 nm. .. NB41A3 cells (50,000 cells/well incubated overnight) were treated for 1, 5, and 15 min with either 10 μM WIN55 or 400 μM N-methyl D-aspartate (Sigma), or mock-treated for 1, 5, and 15 min. Before fixation, cells were incubated with 10 μM CalciumGreen-AM (Invitrogen Molecular Probes) for 1 h. CalciumGreen-AM is a cell permeant ester that is cleaved and sequestered in the cytosol. .. Cell nuclei were labeled with PI.

    Staining:

    Article Title: Tracking NF-?B activity in tumor cells during ovarian cancer progression in a syngeneic mouse model
    Article Snippet: Secondary antibodies used were goat anti-rat or -rabbit or -mouse conjugated with Molecular Probes Alexa488 or Alexa594 (Invitrogen). .. After staining with secondary antibodies, the slides were washed 3 times with TBS-Tween20, then washed once in TBS and then stained with Molecular Probes TO-PRO-3 (Invitrogen) for 15 min (1 μM in TBS). .. The slides were washed with TBS and the coverslips mounted using Molecular Probes ProlongGold antifade reagent (Invitrogen).

    Mutagenesis:

    Article Title: Interaction of the Spo20 Membrane-Sensor Motif with Phosphatidic Acid and Other Anionic Lipids, and Influence of the Membrane Environment
    Article Snippet: For details about the construction of bioprobes based on artificial coiled-coil (ACC1 and ACC2) and their exact nucleotide and amino-acid sequence see . .. NBD labeling of Spo20 NBD labeling of Spo20-GCC and its mutant swapSpo20-GCC, both displaying two endogenous Cys residues (Cys54 and Cys82) flanking the spo20 sensor region, was performed as described for the N-terminal region of GMAP210 , , , using a 10-fold molar excess of N,N′-dimethyl-N-(iodoacetyl)-N′-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine (IANBD amide, Molecular Probes) in dimethylformamide (DMF volume < 5%). ..

    Concentration Assay:

    Article Title: Astaxanthin Restrains Nitrative-Oxidative Peroxidation in Mitochondrial-Mimetic Liposomes: A Pre-Apoptosis Model
    Article Snippet: .. Nitric Oxide Concentration The NO• concentration was measured by the fluorescence intensity (λexc = 495 nm; λem = 515 nm) of the probe 4-amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM; Molecular Probes® , Fisher Scientific, Pittsburgh, PA, USA). .. The manufacturers state that the fluorescence quantum yield (ϕF ) of DAF-FM is c.a.

    Co-Culture Assay:

    Article Title: Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro
    Article Snippet: The fluorescently labeled neutrophils were imaged at day 0, day 1, and day 2 after co-culture with BMSCs using an Olympus XI53 fluorescent microscope (Figure A). .. The effect of neutrophil co-culture on BMSC viability was tested using the Molecular Probes™ LIVE/DEAD™ assay (ThermoFisher Scientific) according to the manufacturer’s protocol. .. Viable cells were fluorescently labeled green and non-viable cells were fluorescently labeled red.

    Live Dead Assay:

    Article Title: Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro
    Article Snippet: The fluorescently labeled neutrophils were imaged at day 0, day 1, and day 2 after co-culture with BMSCs using an Olympus XI53 fluorescent microscope (Figure A). .. The effect of neutrophil co-culture on BMSC viability was tested using the Molecular Probes™ LIVE/DEAD™ assay (ThermoFisher Scientific) according to the manufacturer’s protocol. .. Viable cells were fluorescently labeled green and non-viable cells were fluorescently labeled red.

    Amplification:

    Article Title: Flow Sorting of Marine Bacterioplankton after Fluorescence In Situ Hybridization
    Article Snippet: .. First, samples were hybridized with probe BET42a, and signal amplification was carried out by using tyramides labeled with Alexa 546 (Molecular Probes, Eugene, Oreg.). .. Next, probe-delivered peroxidase was quenched by incubation with 0.01 M HCl for 10 min and rinsing with ddH2 O.

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  • 97
    Thermo Fisher erythrosin er isothiocyanate
    Erythrosin Er Isothiocyanate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erythrosin er isothiocyanate/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    erythrosin er isothiocyanate - by Bioz Stars, 2021-05
    97/100 stars
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