erythrosin b  (Thermo Fisher)


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    Structured Review

    Thermo Fisher erythrosin b
    Inhibition of the NS2B-NS3 interactions and protease activity (A)  Dose-dependent inhibition of SLC upon binding of NLuc-NS2B 49-66  to GST-CLuc-NS3 by erythrosin B. N = 3. (B) Dose-response inhibitions of the DENV2 His-NS2B/His-MBP-NS3 protease activity by erythrosin B. N = 3. ( C ) Dose-response inhibitions of the ZIKV His-NS2B/GST-NS3 protease activity by erythrosin B. N = 3.
    Erythrosin B, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erythrosin b/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    erythrosin b - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease"

    Article Title: Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease

    Journal: Antiviral research

    doi: 10.1016/j.antiviral.2017.12.018

    Inhibition of the NS2B-NS3 interactions and protease activity (A)  Dose-dependent inhibition of SLC upon binding of NLuc-NS2B 49-66  to GST-CLuc-NS3 by erythrosin B. N = 3. (B) Dose-response inhibitions of the DENV2 His-NS2B/His-MBP-NS3 protease activity by erythrosin B. N = 3. ( C ) Dose-response inhibitions of the ZIKV His-NS2B/GST-NS3 protease activity by erythrosin B. N = 3.
    Figure Legend Snippet: Inhibition of the NS2B-NS3 interactions and protease activity (A) Dose-dependent inhibition of SLC upon binding of NLuc-NS2B 49-66 to GST-CLuc-NS3 by erythrosin B. N = 3. (B) Dose-response inhibitions of the DENV2 His-NS2B/His-MBP-NS3 protease activity by erythrosin B. N = 3. ( C ) Dose-response inhibitions of the ZIKV His-NS2B/GST-NS3 protease activity by erythrosin B. N = 3.

    Techniques Used: Inhibition, Activity Assay, Binding Assay

    Inhibition of ZIKV in cells relevant to ZIKV ( A ) qRT-PCR analysis of inhibition of viral RNA from ZIKV infected A549 cells by erythrosin B.  (B)  Immunofluorescence assay (IFA) of inhibition of viral protein expression by erythrosin B, using pan-flavivirus anti-E 4G2 antibody (green) (ATCC). Nuclei (blue) was stained in all IFA assays by the Hoechst stain solution.  (C) ). At 48 h post infection, cells were washed, harvested, and protected by protease inhibitor cocktail prior to lysis by SDS-PAGE loading buffer. Upon incubation at 95 °C for 10 min, sample was subjected to Western blot analysis with anti-ZIKV NS3 (GTX133309, GeneTex, Inc.) and anti-GAPDH (CB1001, EMD Millipore) as primary antibodies. ( D,E ) Viral plaque reduction assay for ZIKV-infected HPECs ( D ) and iPSC-derived hNPCs ( E ) by erythrosin B. ***, p
    Figure Legend Snippet: Inhibition of ZIKV in cells relevant to ZIKV ( A ) qRT-PCR analysis of inhibition of viral RNA from ZIKV infected A549 cells by erythrosin B. (B) Immunofluorescence assay (IFA) of inhibition of viral protein expression by erythrosin B, using pan-flavivirus anti-E 4G2 antibody (green) (ATCC). Nuclei (blue) was stained in all IFA assays by the Hoechst stain solution. (C) ). At 48 h post infection, cells were washed, harvested, and protected by protease inhibitor cocktail prior to lysis by SDS-PAGE loading buffer. Upon incubation at 95 °C for 10 min, sample was subjected to Western blot analysis with anti-ZIKV NS3 (GTX133309, GeneTex, Inc.) and anti-GAPDH (CB1001, EMD Millipore) as primary antibodies. ( D,E ) Viral plaque reduction assay for ZIKV-infected HPECs ( D ) and iPSC-derived hNPCs ( E ) by erythrosin B. ***, p

    Techniques Used: Inhibition, Quantitative RT-PCR, Infection, Immunofluorescence, Expressing, Staining, Protease Inhibitor, Lysis, SDS Page, Incubation, Western Blot, Derivative Assay

    Inhibition of NS2B-NS3 protease complex via a non-competitive mechanism ( A,B ) Inhibition kinetic experiment of the DENV2 His-NS2B/His-MBP-NS3 ( A ) and the ZIKV His-NS2B/GST-NS3 ( B ) heterocomplexes by erythrosin B. Lineweaver–Burk plots of kinetics experimental data for inhibition of the His-NS2B/His-MBP-NS3 hetero protease complexes by erythrosin B. The DENV2 MBP-NS3 or ZIKV GST-NS3 (100 nM) was mixed with erythrosin B (3 μM or various concentrations) for 30 min. The DENV2 His-NS2B or ZIKV His-NS2B (1 μM) were added together with the Abz substrate at various concentrations (800 μM–25 μM in 2-fold dilutions). ( C ) Superimposition of bound EB (yellow) with the NS2B co-factor (cyan) of DENV3 (PDB: 3U1I). NS3 residues in contact with EB were in stick representation (grey). ( D ) Ribbon presentation of erythrosin B (green) docked into NS3pro of DENV3 (PDB: 3U1I). Key interaction residues are highlighted in stick presentation. Hydrogen bonds are shown in yellow dotted lines and π - π stacking is shown in blue line. ( E ) Ribbon presentation of erythrosin B (green) docked into NS3pro of ZIKV (PDB: 5LC0). The corresponding NS3pro β -strand hairpin loops on ZIKV are shown in green and grey. Key interaction residues are highlighted in stick presentation. Hydrogen bonds are also shown in yellow dotted lines and π - π stacking is shown in blue lines.
    Figure Legend Snippet: Inhibition of NS2B-NS3 protease complex via a non-competitive mechanism ( A,B ) Inhibition kinetic experiment of the DENV2 His-NS2B/His-MBP-NS3 ( A ) and the ZIKV His-NS2B/GST-NS3 ( B ) heterocomplexes by erythrosin B. Lineweaver–Burk plots of kinetics experimental data for inhibition of the His-NS2B/His-MBP-NS3 hetero protease complexes by erythrosin B. The DENV2 MBP-NS3 or ZIKV GST-NS3 (100 nM) was mixed with erythrosin B (3 μM or various concentrations) for 30 min. The DENV2 His-NS2B or ZIKV His-NS2B (1 μM) were added together with the Abz substrate at various concentrations (800 μM–25 μM in 2-fold dilutions). ( C ) Superimposition of bound EB (yellow) with the NS2B co-factor (cyan) of DENV3 (PDB: 3U1I). NS3 residues in contact with EB were in stick representation (grey). ( D ) Ribbon presentation of erythrosin B (green) docked into NS3pro of DENV3 (PDB: 3U1I). Key interaction residues are highlighted in stick presentation. Hydrogen bonds are shown in yellow dotted lines and π - π stacking is shown in blue line. ( E ) Ribbon presentation of erythrosin B (green) docked into NS3pro of ZIKV (PDB: 5LC0). The corresponding NS3pro β -strand hairpin loops on ZIKV are shown in green and grey. Key interaction residues are highlighted in stick presentation. Hydrogen bonds are also shown in yellow dotted lines and π - π stacking is shown in blue lines.

    Techniques Used: Inhibition

    Related Articles

    other:

    Article Title: Internal amino acid state modulates yeast taste neurons to support protein homeostasis in Drosophila
    Article Snippet: For the yeast choice assays, the flies were given the choice between nine 10 μl sucrose spots mixed with red colorant (20 mM sucrose [Sigma-Aldrich, St Louis, MO, USA, #84097]; 7.5 mg/ml agarose [Invitrogen, Waltham, MA, USA, #16500]; 5 mg/ml Erythrosin B [Sigma-Aldrich, #198269]; 10% PBS) and nine 10 μl spots of yeast mixed with blue colorant (10% yeast [SAF-instant, Lesaffre, France]; 7.5 mg/ml agarose; 0.25 mg/ml Indigo carmine [Sigma-Aldrich, #131164]; 10% PBS) for 2 hr at 18, 25 or 30°C, 70% RH, depending on the experimental condition.

    Mutagenesis:

    Article Title: Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease
    Article Snippet: The protein thermal-shift assay (PTSA) was conducted using an Applied Biosystem 7500 Fast Real-Time PCR System (ThermoFisher Scientific) from 25 to 80 °C. .. The DENV2 His-MBP-NS3 or each mutant (final concentration of 2.5 μM in 1x PBS) was mixed with erythrosin B to attain a 4.8 μM final concentration in 1.6% DMSO in the MicroAmp® Fast Optical 96-Well Reaction Plate (ThermoFisher Scientific). .. Thermal denaturation was monitored using SYPRO Orange (Life Technologies) according to manufacturer’s manual.

    Concentration Assay:

    Article Title: Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease
    Article Snippet: The protein thermal-shift assay (PTSA) was conducted using an Applied Biosystem 7500 Fast Real-Time PCR System (ThermoFisher Scientific) from 25 to 80 °C. .. The DENV2 His-MBP-NS3 or each mutant (final concentration of 2.5 μM in 1x PBS) was mixed with erythrosin B to attain a 4.8 μM final concentration in 1.6% DMSO in the MicroAmp® Fast Optical 96-Well Reaction Plate (ThermoFisher Scientific). .. Thermal denaturation was monitored using SYPRO Orange (Life Technologies) according to manufacturer’s manual.

    Article Title: Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector
    Article Snippet: .. Erythrosin B (19,826-9, Aldrich Chemical Co, Milwaukee, WI), Rhodamine 6G (RX0090-1, EM Science, Gibbstown, NJ), Ethidium Bromide (E-3565, Molecular Probes, Eugene, OR) and FITC (F-7250, Sigma Chemical Co, St Louis, MO) were used without further purification and after dilution to the appropriate concentration. ..

    Article Title: A method for rapid quantitative assessment of biofilms with biomolecular staining and image analysis
    Article Snippet: .. The stain contained 100 mL of 0.1× concentration phosphate-buffered saline (PBS) at pH 7.4 with 0.1 g of erythrosine B (Thermo Fisher Scientific, Waltham, MA), 0.2 g KeyAcid Rhodamine (Keystone Analine Corporation, Chicago, IL), and 0.3 g Coomassie Brilliant Blue G-250 (Thermo Fisher Scientific) mixed in. .. PBS 1× concentration is comprised of NaCl (8 g L−1 ), KCL (0.2 g L−1 ), Na2 HPO4 (1.44 g L−1 ), and KH2 PO4 (0.24 g L−1 ) with each component purchased from Thermo Fisher Scientific.

    Atomic Absorption Spectroscopy:

    Article Title: Commensal bacteria and essential amino acids control food choice behavior and reproduction
    Article Snippet: .. For the yeast choice assays, the flies were given the choice between nine spots of 10 μl sucrose solution mixed with red colorant (20 mM sucrose [Sigma-Aldrich, #84097]; 7.5 mg/ml agarose [Invitrogen, #16500]; 5 mg/ml Erythrosin B [Sigma-Aldrich, #198269]; 10% PBS) and nine spots of 10 μl yeast solution mixed with blue colorant (10% yeast [Saf-instant, Lesaffre]; 7.5 mg/ml agarose; 0.25 mg/ml Indigo carmine [Sigma-Aldrich, #131164]; 10% PBS) for 2 h. For the defined nutrient-choice assays, flies were given the choice between HM lacking AAs and containing 20 mM sucrose mixed with red colorant (option 1: sucrose) and HM lacking sucrose and containing the nutrients required for the experiment mixed with the blue colorant (option 2). ..

    Purification:

    Article Title: Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector
    Article Snippet: .. Erythrosin B (19,826-9, Aldrich Chemical Co, Milwaukee, WI), Rhodamine 6G (RX0090-1, EM Science, Gibbstown, NJ), Ethidium Bromide (E-3565, Molecular Probes, Eugene, OR) and FITC (F-7250, Sigma Chemical Co, St Louis, MO) were used without further purification and after dilution to the appropriate concentration. ..

    Staining:

    Article Title: A method for rapid quantitative assessment of biofilms with biomolecular staining and image analysis
    Article Snippet: .. The stain contained 100 mL of 0.1× concentration phosphate-buffered saline (PBS) at pH 7.4 with 0.1 g of erythrosine B (Thermo Fisher Scientific, Waltham, MA), 0.2 g KeyAcid Rhodamine (Keystone Analine Corporation, Chicago, IL), and 0.3 g Coomassie Brilliant Blue G-250 (Thermo Fisher Scientific) mixed in. .. PBS 1× concentration is comprised of NaCl (8 g L−1 ), KCL (0.2 g L−1 ), Na2 HPO4 (1.44 g L−1 ), and KH2 PO4 (0.24 g L−1 ) with each component purchased from Thermo Fisher Scientific.

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