er eb2 5 cell line (TaKaRa)


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Er Eb2 5 Cell Line, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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1) Product Images from "Characterization of ABF-1, a Novel Basic Helix-Loop-Helix Transcription Factor Expressed in Activated B Lymphocytes"
Article Title: Characterization of ABF-1, a Novel Basic Helix-Loop-Helix Transcription Factor Expressed in Activated B Lymphocytes
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Figure Legend Snippet: Northern blot analysis of ABF-1 expression. (A) Expression pattern of ABF-1 in human cell lines. Ten micrograms of total RNA was isolated from each cell line and analyzed by Northern blotting. The blot was probed with the ABF-1 cDNA (top), stripped, and subsequently reprobed with the human elongation factor 1 alpha (EF-1α) cDNA (6) as a loading control (bottom). HeLa, carcinoma; Jurkat, T-cell leukemia; 697, pre-B ALL harboring a t(1;19) translocation; Nalm-6, pre-B; BL, Burkitt lymphoma; LCL, lymphoblastoid cell line of the indicated Ig isotype. The ER/EB2-5 cell line was grown in the presence of 1 μM β-estradiol (27). (B) A human tissue Northern blot (Clontech) containing 2 μg of poly(A)+ RNA per lane was sequentially hybridized with ABF-1 (top) and β-actin (bottom). PBL, peripheral blood lymphocyte.
Techniques Used: Northern Blot, Expressing, Isolation, Translocation Assay

Figure Legend Snippet: ABF-1 is part of an E-box binding complex present in EBV-immortalized LCLs. (A) Gel shift analysis with a μE5 probe reveals a novel complex (N) whose mobility differs from that of BCF present in the EBV-immortalized line B3C1 (lane 2). (B) The ABF-1-containing nucleoprotein complex, indicated by arrows, was detected in all EBV-immortalized LCLs (seven cell lines in total were analyzed). Lane 1, unprogrammed reticulocyte lysate; lanes 2 to 5, in vitro-cotranslated ABF-1 and E12 proteins; lanes 6 to 11, nuclear extract derived from the conditional cell line ER/EB2-5 (32) grown in the presence of 1 μM β-estradiol (+est) or estrogen starved for 48 h (−est); lanes 12 to 26, nuclear extract isolated from independent LCLs. N, no antibody; P, preimmune serum; A, ABF-1-specific antiserum. The free probe was run off the gel.
Techniques Used: Binding Assay, Electrophoretic Mobility Shift Assay, In Vitro, Derivative Assay, Isolation