Novus Biologicals
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Proteintech
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Millipore
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Addgene inc
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Novus Biologicals
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Addgene inc
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Molecular Biosciences Inc
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Journal: bioRxiv
Article Title: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer’s disease
doi: 10.1101/2024.07.22.604543
Figure Lengend Snippet: (A) Representative immunofluorescence of ERβ and Iba1 co-staining (arrowheads) in WT (left) and Esr2 -KO (right) male cortex (dotted rectangle: magnified area), and (B) in male App NL-G-F cortex upon vehicle or LY treatment (scale bars = 50 µm). Phagocytosis of Aβ (relative to Cytochalasin D treatment) of primary microglia originating from male (C) and female (D) WT and Esr2 -KO mice. The microglia were treated with or without 10 nM LY 24h prior to and during 24h M1, M2, or no (CTL) activation stimulation (n = 3-4). Transwell migration assay of primary microglia originating from male (E) and female (F) WT and Esr2 -KO mice treated as indicated above (n = 6). Expression of microglial markers Nos2 , Trem2 , Cx3cr1 , P2ry12 , Arg1 , and Cd68 (G-R) relative to housekeeping gene Rplp0 expression in primary microglia originating from male (G, I, K, M, O, Q) and female (H, J, L, N, P, R) WT and Esr2 -KO mice treated as indicated above (n = 3-7). * P < 0.05, ** P < 0.01. Unpaired t-test was used for male microglia and 3-way ANOVA or mixed-effects analysis for female microglia followed by Tukey’s or Dunnett’s multiple comparison test. Overall significant main effects of LY treatment or genotype are indicated.
Article Snippet: Following blocking the slides were immunostained over-night at 4°C with antibodies specific to Aβ (1:2000 dilution, 82E1, IBL-Tecan, Männedorf, Switzerland), Iba1 (1:300, ab178846; and 1:300 ab225260, both from Abcam, Cambridge, UK), GFAP (1:300, GA5 Alexa Fluor 488 -labeled, Millipore), CD68 (1:300, Ab283654, Abcam), and/or
Techniques: Immunofluorescence, Staining, Activation Assay, Transwell Migration Assay, Expressing, Comparison
Journal: Biomedicines
Article Title: The Expression of Adipogenic Marker Is Significantly Increased in Estrogen-Treated Lipedema Adipocytes Differentiated from Adipose Stem Cells In Vitro
doi: 10.3390/biomedicines12051042
Figure Lengend Snippet: List of primers used for qRT-PCR.
Article Snippet: Protein samples were quantified using the bicinchoninic acid assay (BCA, Cat #: 23225; Thermo Fisher), and a total of 0.4 mg of protein lysate was loaded onto the plate along with the following primary antibodies for ERα (1:10; Cat #: AF5715, R&D systems, Minneapolis, MN, USA),
Techniques:
Journal: Biomedicines
Article Title: The Expression of Adipogenic Marker Is Significantly Increased in Estrogen-Treated Lipedema Adipocytes Differentiated from Adipose Stem Cells In Vitro
doi: 10.3390/biomedicines12051042
Figure Lengend Snippet: Expression of estrogen receptors in ASCs. ( A ). qRT-PCR shows a significant increase in ER gene expression in HD-treated healthy and lipedema ASCs ( n = 3). ( B ). Quantification of Western Blot gels shows increased ER protein expression in HD-treated healthy and lipedema ASCs ( n = 3). ( C ). Capillary Western blot (Jess) assay showing ERα, ERβ, and GADPH protein expression in an assembled gel-like image view. Values are means ± SEM. * p < 0.05; ** p < 0.01.
Article Snippet: Protein samples were quantified using the bicinchoninic acid assay (BCA, Cat #: 23225; Thermo Fisher), and a total of 0.4 mg of protein lysate was loaded onto the plate along with the following primary antibodies for ERα (1:10; Cat #: AF5715, R&D systems, Minneapolis, MN, USA),
Techniques: Expressing, Quantitative RT-PCR, Western Blot
Journal: Biomedicines
Article Title: The Expression of Adipogenic Marker Is Significantly Increased in Estrogen-Treated Lipedema Adipocytes Differentiated from Adipose Stem Cells In Vitro
doi: 10.3390/biomedicines12051042
Figure Lengend Snippet: Expression of estrogen receptors in spheroids. ( A ). qRT-PCR shows a significant decrease in ERα and GPER and an increase in ERβ gene expression in HD-treated lipedema spheroids ( n = 3). ( B ). Quantification of Western Blot gels shows increased ERβ protein expression in HD-treated lipedema spheroids ( n = 3). Values are means ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.
Article Snippet: Protein samples were quantified using the bicinchoninic acid assay (BCA, Cat #: 23225; Thermo Fisher), and a total of 0.4 mg of protein lysate was loaded onto the plate along with the following primary antibodies for ERα (1:10; Cat #: AF5715, R&D systems, Minneapolis, MN, USA),
Techniques: Expressing, Quantitative RT-PCR, Western Blot