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Novus Biologicals α erβ
α Erβ, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Representative immunofluorescence of <t>ERβ</t> and Iba1 co-staining (arrowheads) in WT (left) and Esr2 -KO (right) male cortex (dotted rectangle: magnified area), and (B) in male App NL-G-F cortex upon vehicle or LY treatment (scale bars = 50 µm). Phagocytosis of Aβ (relative to Cytochalasin D treatment) of primary microglia originating from male (C) and female (D) WT and Esr2 -KO mice. The microglia were treated with or without 10 nM LY 24h prior to and during 24h M1, M2, or no (CTL) activation stimulation (n = 3-4). Transwell migration assay of primary microglia originating from male (E) and female (F) WT and Esr2 -KO mice treated as indicated above (n = 6). Expression of microglial markers Nos2 , Trem2 , Cx3cr1 , P2ry12 , Arg1 , <t>and</t> <t>Cd68</t> (G-R) relative to housekeeping gene Rplp0 expression in primary microglia originating from male (G, I, K, M, O, Q) and female (H, J, L, N, P, R) WT and Esr2 -KO mice treated as indicated above (n = 3-7). * P < 0.05, ** P < 0.01. Unpaired t-test was used for male microglia and 3-way ANOVA or mixed-effects analysis for female microglia followed by Tukey’s or Dunnett’s multiple comparison test. Overall significant main effects of LY treatment or genotype are indicated.
Erβ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Representative immunofluorescence of <t>ERβ</t> and Iba1 co-staining (arrowheads) in WT (left) and Esr2 -KO (right) male cortex (dotted rectangle: magnified area), and (B) in male App NL-G-F cortex upon vehicle or LY treatment (scale bars = 50 µm). Phagocytosis of Aβ (relative to Cytochalasin D treatment) of primary microglia originating from male (C) and female (D) WT and Esr2 -KO mice. The microglia were treated with or without 10 nM LY 24h prior to and during 24h M1, M2, or no (CTL) activation stimulation (n = 3-4). Transwell migration assay of primary microglia originating from male (E) and female (F) WT and Esr2 -KO mice treated as indicated above (n = 6). Expression of microglial markers Nos2 , Trem2 , Cx3cr1 , P2ry12 , Arg1 , <t>and</t> <t>Cd68</t> (G-R) relative to housekeeping gene Rplp0 expression in primary microglia originating from male (G, I, K, M, O, Q) and female (H, J, L, N, P, R) WT and Esr2 -KO mice treated as indicated above (n = 3-7). * P < 0.05, ** P < 0.01. Unpaired t-test was used for male microglia and 3-way ANOVA or mixed-effects analysis for female microglia followed by Tukey’s or Dunnett’s multiple comparison test. Overall significant main effects of LY treatment or genotype are indicated.
Er Beta Erβ, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore er beta specific inhibitors
(A) Representative immunofluorescence of <t>ERβ</t> and Iba1 co-staining (arrowheads) in WT (left) and Esr2 -KO (right) male cortex (dotted rectangle: magnified area), and (B) in male App NL-G-F cortex upon vehicle or LY treatment (scale bars = 50 µm). Phagocytosis of Aβ (relative to Cytochalasin D treatment) of primary microglia originating from male (C) and female (D) WT and Esr2 -KO mice. The microglia were treated with or without 10 nM LY 24h prior to and during 24h M1, M2, or no (CTL) activation stimulation (n = 3-4). Transwell migration assay of primary microglia originating from male (E) and female (F) WT and Esr2 -KO mice treated as indicated above (n = 6). Expression of microglial markers Nos2 , Trem2 , Cx3cr1 , P2ry12 , Arg1 , <t>and</t> <t>Cd68</t> (G-R) relative to housekeeping gene Rplp0 expression in primary microglia originating from male (G, I, K, M, O, Q) and female (H, J, L, N, P, R) WT and Esr2 -KO mice treated as indicated above (n = 3-7). * P < 0.05, ** P < 0.01. Unpaired t-test was used for male microglia and 3-way ANOVA or mixed-effects analysis for female microglia followed by Tukey’s or Dunnett’s multiple comparison test. Overall significant main effects of LY treatment or genotype are indicated.
Er Beta Specific Inhibitors, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Representative immunofluorescence of <t>ERβ</t> and Iba1 co-staining (arrowheads) in WT (left) and Esr2 -KO (right) male cortex (dotted rectangle: magnified area), and (B) in male App NL-G-F cortex upon vehicle or LY treatment (scale bars = 50 µm). Phagocytosis of Aβ (relative to Cytochalasin D treatment) of primary microglia originating from male (C) and female (D) WT and Esr2 -KO mice. The microglia were treated with or without 10 nM LY 24h prior to and during 24h M1, M2, or no (CTL) activation stimulation (n = 3-4). Transwell migration assay of primary microglia originating from male (E) and female (F) WT and Esr2 -KO mice treated as indicated above (n = 6). Expression of microglial markers Nos2 , Trem2 , Cx3cr1 , P2ry12 , Arg1 , <t>and</t> <t>Cd68</t> (G-R) relative to housekeeping gene Rplp0 expression in primary microglia originating from male (G, I, K, M, O, Q) and female (H, J, L, N, P, R) WT and Esr2 -KO mice treated as indicated above (n = 3-7). * P < 0.05, ** P < 0.01. Unpaired t-test was used for male microglia and 3-way ANOVA or mixed-effects analysis for female microglia followed by Tukey’s or Dunnett’s multiple comparison test. Overall significant main effects of LY treatment or genotype are indicated.
Er β Expression Plasmid Pcdna3 1 Nv5 Er Beta, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals erβ
List of primers used for qRT-PCR.
Erβ, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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List of primers used for qRT-PCR.
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List of primers used for qRT-PCR.
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Image Search Results


(A) Representative immunofluorescence of ERβ and Iba1 co-staining (arrowheads) in WT (left) and Esr2 -KO (right) male cortex (dotted rectangle: magnified area), and (B) in male App NL-G-F cortex upon vehicle or LY treatment (scale bars = 50 µm). Phagocytosis of Aβ (relative to Cytochalasin D treatment) of primary microglia originating from male (C) and female (D) WT and Esr2 -KO mice. The microglia were treated with or without 10 nM LY 24h prior to and during 24h M1, M2, or no (CTL) activation stimulation (n = 3-4). Transwell migration assay of primary microglia originating from male (E) and female (F) WT and Esr2 -KO mice treated as indicated above (n = 6). Expression of microglial markers Nos2 , Trem2 , Cx3cr1 , P2ry12 , Arg1 , and Cd68 (G-R) relative to housekeeping gene Rplp0 expression in primary microglia originating from male (G, I, K, M, O, Q) and female (H, J, L, N, P, R) WT and Esr2 -KO mice treated as indicated above (n = 3-7). * P < 0.05, ** P < 0.01. Unpaired t-test was used for male microglia and 3-way ANOVA or mixed-effects analysis for female microglia followed by Tukey’s or Dunnett’s multiple comparison test. Overall significant main effects of LY treatment or genotype are indicated.

Journal: bioRxiv

Article Title: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer’s disease

doi: 10.1101/2024.07.22.604543

Figure Lengend Snippet: (A) Representative immunofluorescence of ERβ and Iba1 co-staining (arrowheads) in WT (left) and Esr2 -KO (right) male cortex (dotted rectangle: magnified area), and (B) in male App NL-G-F cortex upon vehicle or LY treatment (scale bars = 50 µm). Phagocytosis of Aβ (relative to Cytochalasin D treatment) of primary microglia originating from male (C) and female (D) WT and Esr2 -KO mice. The microglia were treated with or without 10 nM LY 24h prior to and during 24h M1, M2, or no (CTL) activation stimulation (n = 3-4). Transwell migration assay of primary microglia originating from male (E) and female (F) WT and Esr2 -KO mice treated as indicated above (n = 6). Expression of microglial markers Nos2 , Trem2 , Cx3cr1 , P2ry12 , Arg1 , and Cd68 (G-R) relative to housekeeping gene Rplp0 expression in primary microglia originating from male (G, I, K, M, O, Q) and female (H, J, L, N, P, R) WT and Esr2 -KO mice treated as indicated above (n = 3-7). * P < 0.05, ** P < 0.01. Unpaired t-test was used for male microglia and 3-way ANOVA or mixed-effects analysis for female microglia followed by Tukey’s or Dunnett’s multiple comparison test. Overall significant main effects of LY treatment or genotype are indicated.

Article Snippet: Following blocking the slides were immunostained over-night at 4°C with antibodies specific to Aβ (1:2000 dilution, 82E1, IBL-Tecan, Männedorf, Switzerland), Iba1 (1:300, ab178846; and 1:300 ab225260, both from Abcam, Cambridge, UK), GFAP (1:300, GA5 Alexa Fluor 488 -labeled, Millipore), CD68 (1:300, Ab283654, Abcam), and/or ERβ (1:5000, PP-PPZ0506-00, R&D Systems, Minneapolis, MN, USA) (Supplemental Table 1).

Techniques: Immunofluorescence, Staining, Activation Assay, Transwell Migration Assay, Expressing, Comparison

List of primers used for qRT-PCR.

Journal: Biomedicines

Article Title: The Expression of Adipogenic Marker Is Significantly Increased in Estrogen-Treated Lipedema Adipocytes Differentiated from Adipose Stem Cells In Vitro

doi: 10.3390/biomedicines12051042

Figure Lengend Snippet: List of primers used for qRT-PCR.

Article Snippet: Protein samples were quantified using the bicinchoninic acid assay (BCA, Cat #: 23225; Thermo Fisher), and a total of 0.4 mg of protein lysate was loaded onto the plate along with the following primary antibodies for ERα (1:10; Cat #: AF5715, R&D systems, Minneapolis, MN, USA), ERβ (1:10; Cat #: NB200-305, Novus Biologicals, Bio-Techne, Minneapolis, MN, USA), PPARγ (1:100; Cat #: 2443; Cell Signaling Technologies, Danvers, MA, USA), and GAPDH (1:300; Cat #:2118; Cell Signaling Technologies, Danvers, MA, USA).

Techniques:

Expression of estrogen receptors in ASCs. ( A ). qRT-PCR shows a significant increase in ER gene expression in HD-treated healthy and lipedema ASCs ( n = 3). ( B ). Quantification of Western Blot gels shows increased ER protein expression in HD-treated healthy and lipedema ASCs ( n = 3). ( C ). Capillary Western blot (Jess) assay showing ERα, ERβ, and GADPH protein expression in an assembled gel-like image view. Values are means ± SEM. * p < 0.05; ** p < 0.01.

Journal: Biomedicines

Article Title: The Expression of Adipogenic Marker Is Significantly Increased in Estrogen-Treated Lipedema Adipocytes Differentiated from Adipose Stem Cells In Vitro

doi: 10.3390/biomedicines12051042

Figure Lengend Snippet: Expression of estrogen receptors in ASCs. ( A ). qRT-PCR shows a significant increase in ER gene expression in HD-treated healthy and lipedema ASCs ( n = 3). ( B ). Quantification of Western Blot gels shows increased ER protein expression in HD-treated healthy and lipedema ASCs ( n = 3). ( C ). Capillary Western blot (Jess) assay showing ERα, ERβ, and GADPH protein expression in an assembled gel-like image view. Values are means ± SEM. * p < 0.05; ** p < 0.01.

Article Snippet: Protein samples were quantified using the bicinchoninic acid assay (BCA, Cat #: 23225; Thermo Fisher), and a total of 0.4 mg of protein lysate was loaded onto the plate along with the following primary antibodies for ERα (1:10; Cat #: AF5715, R&D systems, Minneapolis, MN, USA), ERβ (1:10; Cat #: NB200-305, Novus Biologicals, Bio-Techne, Minneapolis, MN, USA), PPARγ (1:100; Cat #: 2443; Cell Signaling Technologies, Danvers, MA, USA), and GAPDH (1:300; Cat #:2118; Cell Signaling Technologies, Danvers, MA, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Expression of estrogen receptors in spheroids. ( A ). qRT-PCR shows a significant decrease in ERα and GPER and an increase in ERβ gene expression in HD-treated lipedema spheroids ( n = 3). ( B ). Quantification of Western Blot gels shows increased ERβ protein expression in HD-treated lipedema spheroids ( n = 3). Values are means ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Biomedicines

Article Title: The Expression of Adipogenic Marker Is Significantly Increased in Estrogen-Treated Lipedema Adipocytes Differentiated from Adipose Stem Cells In Vitro

doi: 10.3390/biomedicines12051042

Figure Lengend Snippet: Expression of estrogen receptors in spheroids. ( A ). qRT-PCR shows a significant decrease in ERα and GPER and an increase in ERβ gene expression in HD-treated lipedema spheroids ( n = 3). ( B ). Quantification of Western Blot gels shows increased ERβ protein expression in HD-treated lipedema spheroids ( n = 3). Values are means ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Protein samples were quantified using the bicinchoninic acid assay (BCA, Cat #: 23225; Thermo Fisher), and a total of 0.4 mg of protein lysate was loaded onto the plate along with the following primary antibodies for ERα (1:10; Cat #: AF5715, R&D systems, Minneapolis, MN, USA), ERβ (1:10; Cat #: NB200-305, Novus Biologicals, Bio-Techne, Minneapolis, MN, USA), PPARγ (1:100; Cat #: 2443; Cell Signaling Technologies, Danvers, MA, USA), and GAPDH (1:300; Cat #:2118; Cell Signaling Technologies, Danvers, MA, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot