Journal: Molecular Medicine
Article Title: Progesterone (P4) ameliorates cigarette smoke-induced chronic obstructive pulmonary disease (COPD)
doi: 10.1186/s10020-024-00883-y
Figure Lengend Snippet: P4 protects lung epithelial cells and smooth muscle cells from H 2 O 2 -induced oxidative injury. BEAS-2B and ASM cells were divided into four groups: PBS (control), H 2 O 2 , P4, and H 2 O 2 + P4; cells were treated or co-treated as described and examined for cell viability using MTT assay ( A ); mitochondrial membrane potential by JC-1 staining ( B ); mitochondrial ROS by MitoSOX Green followed by Flow cytometry ( C ); ATP content using ATP Detection Kit ( D ); the apoptosis rate using Annexin-V/PI staining ( E ). N = 3, * p < 0.05, ** p < 0.01 compared to PBS group, † p < 0.05, †† p < 0.01 compared to H 2 O 2 group
Article Snippet: The human lung bronchus epithelial cell line BEAS-2B (CRL-9609) was procured from ATCC (Manassas, VA, USA) and cultured using a Bronchial Epithelial Cell Growth Medium Bullet Kit (Catalog No. CC-3170; Basel, Switzerland).
Techniques: Control, MTT Assay, Membrane, Staining, Flow Cytometry