bronchial epithelial cell growth medium bullet kit  (ATCC)

Journal: Molecular Medicine

Article Title: Progesterone (P4) ameliorates cigarette smoke-induced chronic obstructive pulmonary disease (COPD)

doi: 10.1186/s10020-024-00883-y

Figure Lengend Snippet: P4 protects lung epithelial cells and smooth muscle cells from H 2 O 2 -induced oxidative injury. BEAS-2B and ASM cells were divided into four groups: PBS (control), H 2 O 2 , P4, and H 2 O 2 + P4; cells were treated or co-treated as described and examined for cell viability using MTT assay ( A ); mitochondrial membrane potential by JC-1 staining ( B ); mitochondrial ROS by MitoSOX Green followed by Flow cytometry ( C ); ATP content using ATP Detection Kit ( D ); the apoptosis rate using Annexin-V/PI staining ( E ). N = 3, * p < 0.05, ** p < 0.01 compared to PBS group, † p < 0.05, †† p < 0.01 compared to H 2 O 2 group

Article Snippet: The human lung bronchus epithelial cell line BEAS-2B (CRL-9609) was procured from ATCC (Manassas, VA, USA) and cultured using a Bronchial Epithelial Cell Growth Medium Bullet Kit (Catalog No. CC-3170; Basel, Switzerland).

Techniques: Control, MTT Assay, Membrane, Staining, Flow Cytometry

Journal: Science Advances

Article Title: Epigenetic reprogramming driving successful and failed repair in acute kidney injury

doi: 10.1126/sciadv.ado2849

Figure Lengend Snippet: ( A ) Overview of experimental methodology. Single-nucleus chromatin accessibility atlas was generated from mouse kidneys along the time course after bilateral IRI ( n = 3 to 4 for each time point) and analyzed together with the previously generated snRNA-seq dataset. ( B ) UMAP plot of the previously generated snRNA-seq dataset (left) and newly sequenced snATAC-seq dataset with annotation by label transfer from the snRNA-seq dataset (middle) and clustering-based annotation (right). PT, proximal tubule; PCT, proximal convoluted tubule; PST, proximal straight tubule; PEC, parietal epithelial cells; DTL/ATL, descending/ascending thin limb of Henle’s loop; cTAL/mTAL; cortical/medullary thick ascending limb of Henle’s loop; DCT, distal convoluted tubule; CNT, connecting tubule; PC, principal cells; ICA, type A intercalated cells; ICB, type B intercalated cells; PODO, podocytes; ENDO, endothelial cells; FIB (Fib), fibroblasts; Per, pericytes; Immune, immune cells; URO, uroepithelium. Clustering for snRNA-seq data was performed in our previous study . ( C ) Fragment coverage (frequency of Tn5 insertion) around the DARs around each cell type at lineage marker gene TSSs. Scale bar, 2 kbp.

Article Snippet: Human primary PTCs (Lonza, CC-2553) were cultured with a renal epithelial cell growth medium kit (Lonza, CC-3190) in a humidified 5% CO 2 atmosphere at 37°C.

Techniques: Generated, Marker