Journal: bioRxiv

Article Title: Electrostatic control of chromatin compaction safeguards against apoptotic DNA release

doi: 10.64898/2026.02.23.707452

Figure Lengend Snippet: a , Imaging of chromatin density in the presence and absence of TSA in interphase and apoptosis. Chromatin was stained with the DNA dye SPY650, and Annexin V-488 was used to detect loss of membrane asymmetry in apoptosis. Scale bar 10 µm. b , Quantification of chromatin compaction. n =70 for interphase, n =71 for apoptosis (control); n =73 for interphase, n =68 for apoptosis (TSA). Bars indicate mean; significance was tested by a two-tailed Mann-Whitney test (control apoptosis vs control interphase, P =<0.0001; TSA-treated apoptosis vs control apoptosis, P =<0.0001). c , Imaging of chromatin density in the presence and absence of p300-GFP overexpression in interphase and apoptosis. Chromatin was stained with the DNA dye SPY650, and Annexin V-Pacific Blue was used to detect loss of membrane asymmetry in apoptosis. Scale bar 10 µm. d, e , Quantification of chromatin compaction. n =41 for interphase, n =30 for apoptosis (control); n =19 for interphase, n =18 for apoptosis (p300). Bars indicate mean; significance was tested by a two-tailed Mann-Whitney test (control apoptosis vs control interphase, P =<0.0001; p300 apoptosis vs control apoptosis, P =<0.0001).

Article Snippet: For each condition, a master mix sufficient for seven subsequent reactions was assembled containing 14 μL chromatin (5 μM nucleosome concentration), 12.6 μL of 5X dilution buffer (125 mM Tris pH 7.5, 70 mM KCl, 25 mM DTT, BSA 0.5 mg/mL, 25% Glycerol), 7 μL of 5 μM p300 HAT-domain (produced by Protein Technologies, VBC) in 1X Dilution buffer, and 19.6 μL ddH 2 O.

Techniques: Imaging, Staining, Membrane, Control, Two Tailed Test, MANN-WHITNEY, Over Expression