Journal: Regenerative Therapy

Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

doi: 10.1016/j.reth.2026.101101

Figure Lengend Snippet: MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of CD68-positive cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: For immunophenotyping, single-cell suspensions of both THP-1 and THP-1-M0 cells were labeled with an anti-CD68 antibody (E-AB-F1299L, Elabscience, Wuhan, China).

Techniques: Flow Cytometry, Cell Culture, Derivative Assay, Quantitative RT-PCR, Migration, Transwell Migration Assay

Journal: Regenerative Therapy

Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

doi: 10.1016/j.reth.2026.101101

Figure Lengend Snippet: WTAP silencing inhibited M2 macrophage polarization by regulating MMP12. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. KYSE150 cells were transfected with si-WTAP, MMP12 overexpression plasmid, or the matched control (si-NC and oe-NC). Subsequently, these KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (A) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (B) Flow cytometry was used to quantify the number of CD206-positive macrophages. (C) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: For immunophenotyping, single-cell suspensions of both THP-1 and THP-1-M0 cells were labeled with an anti-CD68 antibody (E-AB-F1299L, Elabscience, Wuhan, China).

Techniques: Transfection, Over Expression, Plasmid Preparation, Control, Cell Culture, Derivative Assay, Quantitative RT-PCR, Flow Cytometry, Migration, Transwell Migration Assay

Journal: BMC Complementary Medicine and Therapies

Article Title: Nano-formulated pomegranate extracts with dual cytotoxic and antimicrobial activity: molecular docking and mechanistic insights into leukemia cell targeting

doi: 10.1186/s12906-026-05291-9

Figure Lengend Snippet: a Average percentage of THP-1 cell viability after treatment with six different pomegranate peel extracts (PPE-Hex, PPE-PE, PPE-EA, PPE-BuOH, PPE-EtOH, and PPE-Aq) at a fixed concentration for 48 h. Cell viability was measured using the MTT assay. The ethyl acetate extract (PPE-EA) showed a significant reduction in viability, like the standard chemotherapeutic agent doxorubicin (DOX). Data are presented as mean ± SD; p < 0.0001 compared to DMEM. b IC₅₀ values of the ethanolic extract (PPE-EA) in crude versus nano-formulated form, determined from serial dilution experiments and calculated by non-linear regression analysis of viability data, with a Bar chart comparing log IC₅₀ values between the crude ethyl acetate extract (PPE-EA) and its nano-formulated counterpart (n-PPE-EA) on THP-1 cells after 48 h of exposure. Nano formulation significantly enhanced cytotoxic potency, reducing the IC₅₀ value from 1.48 µg/mL to 0.191 µg/mL. Statistical significance was determined using an unpaired t-test; p = 0.0001

Article Snippet: To evaluate the oxidative stress status and antioxidant defense response in THP-1 cells following treatment with Punica granatum extracts, malondialdehyde (MDA) levels and catalase enzyme activity were measured using commercial assay kits from Elabscience ( Elabscience Biotechnology Inc. , USA ), following the manufacturer’s protocols.

Techniques: Concentration Assay, MTT Assay, Serial Dilution, Formulation

Journal: BMC Complementary Medicine and Therapies

Article Title: Nano-formulated pomegranate extracts with dual cytotoxic and antimicrobial activity: molecular docking and mechanistic insights into leukemia cell targeting

doi: 10.1186/s12906-026-05291-9

Figure Lengend Snippet: Flow cytometry analysis of ( a ) apoptosis and ( b ) cell cycle distribution in THP-1 cells treated with PPE-EA and n-PPE-EA. Annexin V–FITC/PI dual staining identified four cell populations: A3 = alive cells (Annexin V–/PI–), A4 = early apoptotic cells (Annexin V+/PI–), A2 = Late apoptotic cells (Annexin V+/PI+), A1 = necrotic cells (Annexin V-/PI+). The bar chart summarizes the alive population (A3) and the early apoptotic (A4), late apoptotic (A2), and necrotic (A1) in the three tested groups. Data show increased apoptosis and G2M arrest with nano-formulation. Comparative analysis was conducted using one-way ANOVA followed by Tukey’s post hoc test. a : statistical significance compared to THP-1 cells cultured in DMEM ( p < 0.05), b : statistical significance compared to THP-1 cells cultured in DMEM supplemented with 1.48 µg/mL of PPE-EA ( p < 0.05). Data are presented in mean ± SD. Abbreviations: PPE-EA: ethyl acetate extract from Punica granatum , n-PPE-EA: ethyl acetate extract from Punica granatum in its nano formulation. DMEM: Dulbecco’s Modified Eagle Medium

Article Snippet: To evaluate the oxidative stress status and antioxidant defense response in THP-1 cells following treatment with Punica granatum extracts, malondialdehyde (MDA) levels and catalase enzyme activity were measured using commercial assay kits from Elabscience ( Elabscience Biotechnology Inc. , USA ), following the manufacturer’s protocols.

Techniques: Flow Cytometry, Staining, Formulation, Cell Culture, Modification