mcf 7 cells  (AMS Biotechnology)

Journal: Genes

Article Title: Exploring the Influence of Fok1 / Apa1 Polymorphic Variants on Adolescent Mental Health and Response to Vitamin D Supplementation in Embryonic Hippocampal Cell Lines

doi: 10.3390/genes15070913

Figure Lengend Snippet: Fok1 and Apa1 polymorphic variants in the HN9.10e, HaCat, and MCF7 cell lines.

Article Snippet: The cell MDA–MCF7 line was produced by Elabscience Biotechnology (Houston, TX, USA) [ ].

Techniques:

Journal: Genes

Article Title: Exploring the Influence of Fok1 / Apa1 Polymorphic Variants on Adolescent Mental Health and Response to Vitamin D Supplementation in Embryonic Hippocampal Cell Lines

doi: 10.3390/genes15070913

Figure Lengend Snippet: Effect of 100 nM VitD3 treatment on VDR gene expression in HN9.10e, HaCat, and MCF7 cell lines. GAPDH and 18S rRNA were used as housekeeping genes. Relative mRNA expression levels were calculated as 2 −ΔΔCt , comparing the results of the treated samples with the untreated sample (control sample) equal to 1, the origin of the axes. Data are expressed as the mean ± SD of 3 independent experiments performed in duplicate. * p < 0.05 versus control sample.

Article Snippet: The cell MDA–MCF7 line was produced by Elabscience Biotechnology (Houston, TX, USA) [ ].

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

doi: 10.3390/ijms242417263

Figure Lengend Snippet: Effect of increasing doses of VitC on MCF7 and MB231 cell viability. Data are expressed as percentages with respect to the control sample (CTR) (100%) and represent the mean ± SD of 3 independent experiments performed in duplicate. DMSO treatment was used as a positive CTR. * p < 0.05 versus CTR.

Article Snippet: The cell lines MDA-MCF7 and MDA-MB-231 were produced by Elabscience Biotechnology (Houston, TX, USA).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

doi: 10.3390/ijms242417263

Figure Lengend Snippet: Effect of 0.1 mM and 10 mM Vitamin C on the gene expression of GADD45A coding growth arrest and DNA-damage-inducible alpha protein, CCND1 coding cyclin D1 protein, and CDKN1A gene coding cyclin-dependent kinase inhibitor 1A protein in MCF7 and MB231 cells. GAPDH and 18S rRNA were used as housekeeping genes. mRNA relative expression levels were calculated as 2 −ΔΔCt , comparing the results of the treated samples with the control sample (CTR) equal to 1, the origin of the axes. Data are expressed as the mean ± SD of 3 independent experiments performed in duplicate. * p < 0.05 versus CTR.

Article Snippet: The cell lines MDA-MCF7 and MDA-MB-231 were produced by Elabscience Biotechnology (Houston, TX, USA).

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

doi: 10.3390/ijms242417263

Figure Lengend Snippet: Effect of 0.1 mM and 10 mM Vitamin C on MCF7 cell phenotype. Immunocytochemistry analysis of ER, PR, Ki67, and HER2. Data represent the mean ± SD of ten slide readings from 2 experiments performed in duplicate. * p < 0.05 versus control sample (CTR).

Article Snippet: The cell lines MDA-MCF7 and MDA-MB-231 were produced by Elabscience Biotechnology (Houston, TX, USA).

Techniques: Immunocytochemistry, Control

Journal: International Journal of Molecular Sciences

Article Title: Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

doi: 10.3390/ijms242417263

Figure Lengend Snippet: Sphingomyelin (SM) and ceramide (CER) species in MCF7 ( a , b ) and MB231 ( c , d ) cells treated with 0.1 mM and 10 mM VitC. Data are expressed as the mean ± SD of 3 independent experiments performed in duplicate. * p < 0.05 versus control sample (CTR).

Article Snippet: The cell lines MDA-MCF7 and MDA-MB-231 were produced by Elabscience Biotechnology (Houston, TX, USA).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

doi: 10.3390/ijms242417263

Figure Lengend Snippet: Total sphingomyelin (SM) and ceramide (CER) species in ( a ) MCF7 and ( b ) MB231 cells treated with 0.1 mM and 10 mM VitC. ( c ) Sum of SM and CER species in MCF7 and MB231 cells. Data are expressed as the mean ± SD of 3 independent experiments performed in duplicate. * p < 0.05 versus control sample (CTR).

Article Snippet: The cell lines MDA-MCF7 and MDA-MB-231 were produced by Elabscience Biotechnology (Houston, TX, USA).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

doi: 10.3390/ijms242417263

Figure Lengend Snippet: Presence of sphingomyelin in control (CTR), 0.1 mM, and 10 mM treated MCF7 and Mb231 cells with the EGFP-NT-Lys fluorescent probe.

Article Snippet: The cell lines MDA-MCF7 and MDA-MB-231 were produced by Elabscience Biotechnology (Houston, TX, USA).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

doi: 10.3390/ijms242417263

Figure Lengend Snippet: Effect of 0.1 mM and 10 mM Vitamin C on the gene expression of SMPD1 coding for acid sphingomyelinase and SMPD2 coding for neutral sphingomyelinase 1 in ( a ) MCF7 and ( b ) MB231 cells. GAPDH and 18S rRNA were used as housekeeping genes. mRNA relative expression levels were calculated as 2 −ΔΔCt , comparing the results of the treated samples with the control sample (CTR) equal to 1, the origin of the axes. Data are expressed as the mean ± SD of 3 independent experiments performed in duplicate. * p < 0.05 versus CTR.

Article Snippet: The cell lines MDA-MCF7 and MDA-MB-231 were produced by Elabscience Biotechnology (Houston, TX, USA).

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

doi: 10.3390/ijms242417263

Figure Lengend Snippet: Effect of 0.1 mM and 10 mM Vitamin C on acid sphingomyelinase (aSMase) and neutral sphingomyelinase1 (nSMase) in MCF7 and MB231 cells. ( a ) Western blotting panel of aSMase and nSMase1 and actin as reference; ( b ) densitometric analysis of MCF7, the values were normalized with actin and were expressed as a percentage of the control sample (CTR). ( c ) densitometric analysis of MB231, the values were normalized with actin and were expressed as a percentage of the control sample (CTR). Data are expressed as the mean ± SD of 3 independent experiments performed in duplicate. * p < 0.05 versus CTR.

Article Snippet: The cell lines MDA-MCF7 and MDA-MB-231 were produced by Elabscience Biotechnology (Houston, TX, USA).

Techniques: Western Blot, Control