Journal: Coatings
Article Title: Starch/Poly(glycerol-adipate) Nanocomposites: A Novel Oral Drug Delivery Device
doi: 10.3390/coatings10020125
Figure Lengend Snippet: Figure 3. In vitro cytotoxicity of study formulations on Caco-2 (a) and HCT116 (b) intestinal epithelial cells. Formulations were applied to cells at a polymer concentration of 0.5 mg/mL diluted in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% Fetal Bovine Serum (FBS) for 48 h. A total of 1% (v/v) Triton X-100 (TX-100) was used as the positive cell death control. Caco-2 and HCT116 cells were seeded on 96 well plates at a density of 1 × 104 and 2 × 104 cells/well, respectively, and were cultured for 24 h prior to assaying. Metabolic activity was assessed using the PrestoBlue assay. The data represent mean ± S.D. (n = 3).
Article Snippet: Caco-2 human epithelial colorectal adenocarcinoma cells were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA), and were used between passages 30-35 and HCT116 colorectal cancer cells were obtained from AMS Biotechnology (Abingdon, UK).
Techniques: In Vitro, Polymer, Concentration Assay, Control, Cell Culture, Activity Assay, Prestoblue Assay