enzyme mmei  (New England Biolabs)


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    Structured Review

    New England Biolabs enzyme mmei
    Enzyme Mmei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme mmei/product/New England Biolabs
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme mmei - by Bioz Stars, 2020-04
    89/100 stars

    Related Products / Commonly Used Together

    cdna tags
    primer extension

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    Related Articles

    Paired-end Tag:

    Article Title: aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells
    Article Snippet: The cDNA tags were released by digestion with 2–8 U of the tagging enzyme MmeI (NEB, Frankfurt a.M., Germany) in 200 μl of the supplied buffer in the presence of 50 μM S -adenosylmethionine at 37°C for 1 h. The longSAGE tags were ligated as described by Saha et al . , diluted (1:175) and amplified with non-biotinylated primers for 26 cycles. .. Following the electroelution, the ditags were PC8 extracted, ethanol precipitated and resuspended in 7 μl of loTE (3 mM Tris–HCl, pH 7.5; 0.3 mM EDTA).

    Article Title: Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2
    Article Snippet: The ligated DNA was digested with tagging enzyme MmeI (NEB). .. The cleaved tags were ligated to form ditags and amplified by PCR.

    Amplification:

    Article Title: aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells
    Article Snippet: .. The cDNA tags were released by digestion with 2–8 U of the tagging enzyme MmeI (NEB, Frankfurt a.M., Germany) in 200 μl of the supplied buffer in the presence of 50 μM S -adenosylmethionine at 37°C for 1 h. The longSAGE tags were ligated as described by Saha et al . , diluted (1:175) and amplified with non-biotinylated primers for 26 cycles. .. All SAGE libraries were prepared from 96 pooled PCR reactions, purified by PC8 extraction and ethanol precipitation and digested with 200 U Hsp92II (Promega) in the buffer provided with the enzyme in a reaction volume of 200 μl for 1 h at 37°C.

    Article Title: Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2
    Article Snippet: Briefly, amplified single-cell PCR products were digested with anchoring enzyme NlaIII (New England BioLabs). .. The ligated DNA was digested with tagging enzyme MmeI (NEB).

    Agarose Gel Electrophoresis:

    Article Title: Mapping of transcription start sites in Saccharomyces cerevisiae using 5? SAGE
    Article Snippet: The primer extension products are checked by electrophoresis on 2% agarose gel (Supplementary Figure S1A). .. The two primer extension products are digested by tagging enzyme MmeI (New England Biolabs) for 2 h at 37°C.

    Magnetic Beads:

    Article Title: Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2
    Article Snippet: Cleaved samples were divided into two fractions and were bound to streptavidin magnetic beads (Dynal), and linkers were ligated. .. The ligated DNA was digested with tagging enzyme MmeI (NEB).

    Ethanol Precipitation:

    Article Title: aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells
    Article Snippet: The cDNA tags were released by digestion with 2–8 U of the tagging enzyme MmeI (NEB, Frankfurt a.M., Germany) in 200 μl of the supplied buffer in the presence of 50 μM S -adenosylmethionine at 37°C for 1 h. The longSAGE tags were ligated as described by Saha et al . , diluted (1:175) and amplified with non-biotinylated primers for 26 cycles. .. All SAGE libraries were prepared from 96 pooled PCR reactions, purified by PC8 extraction and ethanol precipitation and digested with 200 U Hsp92II (Promega) in the buffer provided with the enzyme in a reaction volume of 200 μl for 1 h at 37°C.

    Synthesized:

    Article Title: Mapping of transcription start sites in Saccharomyces cerevisiae using 5? SAGE
    Article Snippet: The newly synthesized single strand cDNA is used as template for three cycles primer extension by PCR. .. The two primer extension products are digested by tagging enzyme MmeI (New England Biolabs) for 2 h at 37°C.

    Ligation:

    Article Title: aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells
    Article Snippet: After the second strand synthesis, the cDNA was digested with 50 U of the anchoring enzyme Hsp92II (an isoschizomer of NlaIII, Promega) in the buffer provided by the manufacturer in a reaction volume of 200 μl at 37°C for 1 h. The volume of the linker ligation was reduced to 10 μl. .. The cDNA tags were released by digestion with 2–8 U of the tagging enzyme MmeI (NEB, Frankfurt a.M., Germany) in 200 μl of the supplied buffer in the presence of 50 μM S -adenosylmethionine at 37°C for 1 h. The longSAGE tags were ligated as described by Saha et al . , diluted (1:175) and amplified with non-biotinylated primers for 26 cycles.

    Purification:

    Article Title: aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells
    Article Snippet: The cDNA tags were released by digestion with 2–8 U of the tagging enzyme MmeI (NEB, Frankfurt a.M., Germany) in 200 μl of the supplied buffer in the presence of 50 μM S -adenosylmethionine at 37°C for 1 h. The longSAGE tags were ligated as described by Saha et al . , diluted (1:175) and amplified with non-biotinylated primers for 26 cycles. .. All SAGE libraries were prepared from 96 pooled PCR reactions, purified by PC8 extraction and ethanol precipitation and digested with 200 U Hsp92II (Promega) in the buffer provided with the enzyme in a reaction volume of 200 μl for 1 h at 37°C.

    Electrophoresis:

    Article Title: Mapping of transcription start sites in Saccharomyces cerevisiae using 5? SAGE
    Article Snippet: The primer extension products are checked by electrophoresis on 2% agarose gel (Supplementary Figure S1A). .. The two primer extension products are digested by tagging enzyme MmeI (New England Biolabs) for 2 h at 37°C.

    Concentration Assay:

    Article Title: Mapping of transcription start sites in Saccharomyces cerevisiae using 5? SAGE
    Article Snippet: First, CDS primer [5′-AAGCAGTGGTATCAACGCAGAGTAC-(T)30 -VN-3′] (V = A, G or C) is added with either 5′ SAGE template switching (TS) oligos A (5′-GGGATTTGCTGGTGCAGTACAGGATCCGACggg-3′; lower case, RNA) or B (5′-GCTGCTCGAATTCAAGCTTCTGGATCCGACggg-3′; lower case, RNA) in the reaction mixture for first strand synthesis and TS at 42°C for 90 min. Each oligo final concentration is 1 μM. .. The two primer extension products are digested by tagging enzyme MmeI (New England Biolabs) for 2 h at 37°C.

    Incubation:

    Article Title: aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells
    Article Snippet: Oligo(dT) beads and total RNA were incubated for 5 min at room temperature rotating on an overhead shaker. .. The cDNA tags were released by digestion with 2–8 U of the tagging enzyme MmeI (NEB, Frankfurt a.M., Germany) in 200 μl of the supplied buffer in the presence of 50 μM S -adenosylmethionine at 37°C for 1 h. The longSAGE tags were ligated as described by Saha et al . , diluted (1:175) and amplified with non-biotinylated primers for 26 cycles.

    Clone Assay:

    Article Title: Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2
    Article Snippet: The ligated DNA was digested with tagging enzyme MmeI (NEB). .. The concatemers were cloned into pZero-1 vector (Invitrogen).

    Expressing:

    Article Title: Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2
    Article Snippet: Amplified PCR products were screened for expression of Gapdh , Omp , mCherry , Adcy3 , Cnga2 and Gnao with gene-specific primers. .. The ligated DNA was digested with tagging enzyme MmeI (NEB).

    Polymerase Chain Reaction:

    Article Title: Mapping of transcription start sites in Saccharomyces cerevisiae using 5? SAGE
    Article Snippet: The newly synthesized single strand cDNA is used as template for three cycles primer extension by PCR. .. The two primer extension products are digested by tagging enzyme MmeI (New England Biolabs) for 2 h at 37°C.

    Article Title: aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells
    Article Snippet: The cDNA tags were released by digestion with 2–8 U of the tagging enzyme MmeI (NEB, Frankfurt a.M., Germany) in 200 μl of the supplied buffer in the presence of 50 μM S -adenosylmethionine at 37°C for 1 h. The longSAGE tags were ligated as described by Saha et al . , diluted (1:175) and amplified with non-biotinylated primers for 26 cycles. .. All SAGE libraries were prepared from 96 pooled PCR reactions, purified by PC8 extraction and ethanol precipitation and digested with 200 U Hsp92II (Promega) in the buffer provided with the enzyme in a reaction volume of 200 μl for 1 h at 37°C.

    Article Title: Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2
    Article Snippet: Briefly, amplified single-cell PCR products were digested with anchoring enzyme NlaIII (New England BioLabs). .. The ligated DNA was digested with tagging enzyme MmeI (NEB).

    Staining:

    Article Title: Mapping of transcription start sites in Saccharomyces cerevisiae using 5? SAGE
    Article Snippet: The two primer extension products are digested by tagging enzyme MmeI (New England Biolabs) for 2 h at 37°C. .. After staining with ethidium bromide, the ∼60 bp tag band from two pools are combined and eluted from the gel.

    Plasmid Preparation:

    Article Title: Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2
    Article Snippet: The ligated DNA was digested with tagging enzyme MmeI (NEB). .. The concatemers were cloned into pZero-1 vector (Invitrogen).

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  • 90
    New England Biolabs mmei restriction enzyme
    Mmei Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmei restriction enzyme/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mmei restriction enzyme - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    89
    New England Biolabs enzyme mmei
    Enzyme Mmei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme mmei/product/New England Biolabs
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    enzyme mmei - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

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