enzyme linked immunosorbent assay elisa method  (Millipore)


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    Name:
    Rat Leptin ELISA Kit
    Description:
    The Rat Leptin ELISA Enzyme Linked Immunosorbent Assay kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of rat Leptin in serum plasma cell culture supernatants and urine
    Catalog Number:
    rab0335
    Price:
    None
    Applications:
    For research use only. Not for use in diagnostic procedures.Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
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    Structured Review

    Millipore enzyme linked immunosorbent assay elisa method
    Rat Leptin ELISA Kit
    The Rat Leptin ELISA Enzyme Linked Immunosorbent Assay kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of rat Leptin in serum plasma cell culture supernatants and urine
    https://www.bioz.com/result/enzyme linked immunosorbent assay elisa method/product/Millipore
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    enzyme linked immunosorbent assay elisa method - by Bioz Stars, 2020-09
    95/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Gestational high fat diet programs hepatic phosphoenolpyruvate carboxykinase gene expression and histone modification in neonatal offspring rats
    Article Snippet: .. Maternal plasma leptin was analysed using the Rat Leptin ELISA Kit (Millipore cat. No. EZRL-83K, Billerica, MA, USA) according to the manufacturer's instructions. .. Maternal serum insulin and MCP-1 were analysed using the Milliplex MAP Rat Serum Adipokine Panel Kit from Millipore (cat. no. RADPK-81K) following the manufacturer's instructions and results were obtained using the Bio-Plex 200 System from Bio-Rad (Hercules, CA, USA).

    Article Title: A 72-hour high fat diet increases transcript levels of the neuropeptide galanin in the dorsal hippocampus of the rat
    Article Snippet: .. Serum leptin was measured using a rat-specific ELISA kit (EZRL-83K; Millipore; Billerica, MA, USA). .. Total serum cholesterol was determined using a cholesterol reagent set (C7510; Pointe Scientific; Ann Arbor, MI, USA).

    Article Title: The Molecular Mechanism of Leptin Secretion and Expression Induced by Aristolochic Acid in Kidney Fibroblast
    Article Snippet: .. The amount of leptin secretion into the culture medium was determined by enzyme-linked immunosorbent assay (ELISA) using a commercially available rat leptin ELISA kit (Millipore, Bedford, MA) according to the protocol recommended by the manufacturer. ..

    Article Title: Neuroglial metabolic compartmentation underlying leptin deficiency in the obese ob/ob mice as detected by magnetic resonance imaging and spectroscopy methods
    Article Snippet: .. Plasma insulin or leptin levels were determined using commercially available ELISA kits (Millipore Corporation, Billerica, MA, USA or LINCO Research, St Charles, MO, USA). .. Brain volumes were determined in each animal from quantitative volumetry studies of T1 -weighted MRI sections as described below.

    Article Title: Diminished satellite cells and elevated adipogenic gene expression in muscle as caused by ovariectomy are averted by low-magnitude mechanical signals
    Article Snippet: .. Serum leptin and adiponectin levels were evaluated with commercial ELISA kits (Mouse Leptin ELISA and Mouse Adiponectin ELISA; EMD Millipore, St. Charles, MO), and standard curves were determined according to the manufacturer's protocols. ..

    Article Title: The Molecular Mechanism of Leptin Secretion and Expression Induced by Aristolochic Acid in Kidney Fibroblast
    Article Snippet: .. Rat leptin ELISA kit was purchased from Millipore (Bedford, MA). .. Anti-leptin antibody, anti β-actin antibody, anti-C/EBP α antibody, anti-Akt antibody and Akt siRNA were obtained from Santa Cruz (Santa Cruz, CA, USA).

    Article Title: Intermittent Fasting Dietary Restriction Regimen Negatively Influences Reproduction in Young Rats: A Study of Hypothalamo-Hypophysial-Gonadal Axis
    Article Snippet: .. Leptin and LH ELISA The serum leptin and LH concentration from both female and male rat (n = 6 each in duplicate) groups was determined using rat Leptin ELISA kit (Millipore, USA) and rat LH ELISA kit (Cusabio, China) according to the manufacturer’s instructions using Multiscan Ascent Microplate Reader (Thermo Electron Corporation). .. The concentration of serum leptin (ng/ml) and LH (mlU/ml) was then calculated using kit provided standards and Sigma Stat software.

    Article Title: Pharmaceutically controlled designer circuit for the treatment of the metabolic syndrome
    Article Snippet: .. After 3 d the treated animals were fasted for 4 h (unless otherwise indicated) and their body weight was determined before they were killed to profile the blood levels of free fatty acids (NEFA-HR[2] kits; Wako Chemical GmbH), cholesterol (LabAssay Cholesterol kit; Wako Chemical GmbH), insulin (mouse insulin ELISA kit; Mercodia), GLP-1 (active GLP-1 ELISA kit; Millipore), and leptin (leptin ELISA kit; Millipore). ..

    Concentration Assay:

    Article Title: Intermittent Fasting Dietary Restriction Regimen Negatively Influences Reproduction in Young Rats: A Study of Hypothalamo-Hypophysial-Gonadal Axis
    Article Snippet: .. Leptin and LH ELISA The serum leptin and LH concentration from both female and male rat (n = 6 each in duplicate) groups was determined using rat Leptin ELISA kit (Millipore, USA) and rat LH ELISA kit (Cusabio, China) according to the manufacturer’s instructions using Multiscan Ascent Microplate Reader (Thermo Electron Corporation). .. The concentration of serum leptin (ng/ml) and LH (mlU/ml) was then calculated using kit provided standards and Sigma Stat software.

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  • 95
    Millipore enzyme linked immunosorbent assay elisa method
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