enzyme hotstar taq polymerase  (Qiagen)


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    Structured Review

    Qiagen enzyme hotstar taq polymerase
    Enzyme Hotstar Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme hotstar taq polymerase/product/Qiagen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme hotstar taq polymerase - by Bioz Stars, 2020-04
    85/100 stars

    Related Products / Commonly Used Together

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    hotstar taq pcr buffer

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    Related Articles

    Amplification:

    Article Title: Novel IL10 gene family associations with systemic juvenile idiopathic arthritis
    Article Snippet: Primers designed by RealSNP assay (Sequenom) amplified approximately 100 bp of sequence surrounding the target SNP. .. PCR was carried out using 1× HotStar Taq PCR buffer 2.5 mM MgCl2 (Qiagen, Crawley, West Sussex, UK), 500 μM of each dNTP, 0.1 U Enzyme HotStar Taq polymerase (Qiagen), 100 nM primer, and 2.5 ng of genomic DNA in a total volume of 5 μl.

    Purification:

    Article Title: Novel IL10 gene family associations with systemic juvenile idiopathic arthritis
    Article Snippet: PCR was carried out using 1× HotStar Taq PCR buffer 2.5 mM MgCl2 (Qiagen, Crawley, West Sussex, UK), 500 μM of each dNTP, 0.1 U Enzyme HotStar Taq polymerase (Qiagen), 100 nM primer, and 2.5 ng of genomic DNA in a total volume of 5 μl. .. Finally, the extended product was purified of excess ions using an ion-exchange resin.

    Polymerase Chain Reaction:

    Article Title: Novel IL10 gene family associations with systemic juvenile idiopathic arthritis
    Article Snippet: .. PCR was carried out using 1× HotStar Taq PCR buffer 2.5 mM MgCl2 (Qiagen, Crawley, West Sussex, UK), 500 μM of each dNTP, 0.1 U Enzyme HotStar Taq polymerase (Qiagen), 100 nM primer, and 2.5 ng of genomic DNA in a total volume of 5 μl. .. PCR was followed by incubation with shrimp alkaline phosphatase to digest unincorporated dNTPs and primers.

    Incubation:

    Article Title: Novel IL10 gene family associations with systemic juvenile idiopathic arthritis
    Article Snippet: PCR was carried out using 1× HotStar Taq PCR buffer 2.5 mM MgCl2 (Qiagen, Crawley, West Sussex, UK), 500 μM of each dNTP, 0.1 U Enzyme HotStar Taq polymerase (Qiagen), 100 nM primer, and 2.5 ng of genomic DNA in a total volume of 5 μl. .. PCR was followed by incubation with shrimp alkaline phosphatase to digest unincorporated dNTPs and primers.

    Sequencing:

    Article Title: Novel IL10 gene family associations with systemic juvenile idiopathic arthritis
    Article Snippet: Primers designed by RealSNP assay (Sequenom) amplified approximately 100 bp of sequence surrounding the target SNP. .. PCR was carried out using 1× HotStar Taq PCR buffer 2.5 mM MgCl2 (Qiagen, Crawley, West Sussex, UK), 500 μM of each dNTP, 0.1 U Enzyme HotStar Taq polymerase (Qiagen), 100 nM primer, and 2.5 ng of genomic DNA in a total volume of 5 μl.

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