enzyme compatible solvent system isopropanol nonidat p40  (Roche)

 
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    Structured Review

    Roche enzyme compatible solvent system isopropanol nonidat p40
    Enzyme Compatible Solvent System Isopropanol Nonidat P40, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme compatible solvent system isopropanol nonidat p40/product/Roche
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme compatible solvent system isopropanol nonidat p40 - by Bioz Stars, 2020-03
    84/100 stars

    Related Products / Commonly Used Together

    eppendorf microtubes
    suction evaporation

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    Related Articles

    In Vitro:

    Article Title: Nicotinic Acid Receptor GPR109A Is Down-Regulated in Human Macrophage-Derived Foam Cells
    Article Snippet: Paragraph title: In vitro Cholesterol Loading and Efflux ... This solvent mixture was then transferred to 1.5 mL Eppendorf microtubes for suction evaporation with the dried cholesterol residue reconstituted in an enzyme-compatible solvent system isopropanol:Nonidat P40 (9∶1, Roche, Burgess Hill, UK), supplemented with 20 U/mL of bovine catalase (Sigma-Aldrich, Poole, UK) to quench intrinsic peroxide activity.

    Amplex Red Cholesterol Assay:

    Article Title: Nicotinic Acid Receptor GPR109A Is Down-Regulated in Human Macrophage-Derived Foam Cells
    Article Snippet: Cholesterol efflux was assessed using HDL3 (50 µg/mL, Meridian Life Science, USA) and apolipoprotein (Apo) AI (10 µg/mL, Sigma-Aldrich, Poole, UK) as cholesterol acceptors and a non-radioactive enzymatic method (Amplex Red Cholesterol Assay kit, Invitrogen, Paisley, UK) modified from Robinet, et al. . .. This solvent mixture was then transferred to 1.5 mL Eppendorf microtubes for suction evaporation with the dried cholesterol residue reconstituted in an enzyme-compatible solvent system isopropanol:Nonidat P40 (9∶1, Roche, Burgess Hill, UK), supplemented with 20 U/mL of bovine catalase (Sigma-Aldrich, Poole, UK) to quench intrinsic peroxide activity.

    Cell Culture:

    Article Title: Nicotinic Acid Receptor GPR109A Is Down-Regulated in Human Macrophage-Derived Foam Cells
    Article Snippet: In brief, after the indicated treatment period, cell culture supernatants were collected and stored for later analysis; while foam cells were washed in cold PBS twice and cellular cholesterol extracted by incubation with hexane:isopropanol (3∶2) solvent mixture with gentle agitation for 30 minutes. .. This solvent mixture was then transferred to 1.5 mL Eppendorf microtubes for suction evaporation with the dried cholesterol residue reconstituted in an enzyme-compatible solvent system isopropanol:Nonidat P40 (9∶1, Roche, Burgess Hill, UK), supplemented with 20 U/mL of bovine catalase (Sigma-Aldrich, Poole, UK) to quench intrinsic peroxide activity.

    Evaporation:

    Article Title: Nicotinic Acid Receptor GPR109A Is Down-Regulated in Human Macrophage-Derived Foam Cells
    Article Snippet: .. This solvent mixture was then transferred to 1.5 mL Eppendorf microtubes for suction evaporation with the dried cholesterol residue reconstituted in an enzyme-compatible solvent system isopropanol:Nonidat P40 (9∶1, Roche, Burgess Hill, UK), supplemented with 20 U/mL of bovine catalase (Sigma-Aldrich, Poole, UK) to quench intrinsic peroxide activity. .. Extracted cellular and supernatant cholesterol content was then determined using Amplex Red Cholesterol Assay kit (Invitrogen, Paisley, UK) as per manufacturer’s instruction.

    Incubation:

    Article Title: Nicotinic Acid Receptor GPR109A Is Down-Regulated in Human Macrophage-Derived Foam Cells
    Article Snippet: In brief, after the indicated treatment period, cell culture supernatants were collected and stored for later analysis; while foam cells were washed in cold PBS twice and cellular cholesterol extracted by incubation with hexane:isopropanol (3∶2) solvent mixture with gentle agitation for 30 minutes. .. This solvent mixture was then transferred to 1.5 mL Eppendorf microtubes for suction evaporation with the dried cholesterol residue reconstituted in an enzyme-compatible solvent system isopropanol:Nonidat P40 (9∶1, Roche, Burgess Hill, UK), supplemented with 20 U/mL of bovine catalase (Sigma-Aldrich, Poole, UK) to quench intrinsic peroxide activity.

    Activity Assay:

    Article Title: Nicotinic Acid Receptor GPR109A Is Down-Regulated in Human Macrophage-Derived Foam Cells
    Article Snippet: .. This solvent mixture was then transferred to 1.5 mL Eppendorf microtubes for suction evaporation with the dried cholesterol residue reconstituted in an enzyme-compatible solvent system isopropanol:Nonidat P40 (9∶1, Roche, Burgess Hill, UK), supplemented with 20 U/mL of bovine catalase (Sigma-Aldrich, Poole, UK) to quench intrinsic peroxide activity. .. Extracted cellular and supernatant cholesterol content was then determined using Amplex Red Cholesterol Assay kit (Invitrogen, Paisley, UK) as per manufacturer’s instruction.

    Modification:

    Article Title: Nicotinic Acid Receptor GPR109A Is Down-Regulated in Human Macrophage-Derived Foam Cells
    Article Snippet: Cholesterol efflux was assessed using HDL3 (50 µg/mL, Meridian Life Science, USA) and apolipoprotein (Apo) AI (10 µg/mL, Sigma-Aldrich, Poole, UK) as cholesterol acceptors and a non-radioactive enzymatic method (Amplex Red Cholesterol Assay kit, Invitrogen, Paisley, UK) modified from Robinet, et al. . .. This solvent mixture was then transferred to 1.5 mL Eppendorf microtubes for suction evaporation with the dried cholesterol residue reconstituted in an enzyme-compatible solvent system isopropanol:Nonidat P40 (9∶1, Roche, Burgess Hill, UK), supplemented with 20 U/mL of bovine catalase (Sigma-Aldrich, Poole, UK) to quench intrinsic peroxide activity.

    Staining:

    Article Title: Nicotinic Acid Receptor GPR109A Is Down-Regulated in Human Macrophage-Derived Foam Cells
    Article Snippet: Foam cell formation was confirmed by Oil-red-O (Sigma-Aldrich, Poole, UK) staining in sample wells. .. This solvent mixture was then transferred to 1.5 mL Eppendorf microtubes for suction evaporation with the dried cholesterol residue reconstituted in an enzyme-compatible solvent system isopropanol:Nonidat P40 (9∶1, Roche, Burgess Hill, UK), supplemented with 20 U/mL of bovine catalase (Sigma-Aldrich, Poole, UK) to quench intrinsic peroxide activity.

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  • 84
    Roche enzyme compatible solvent system isopropanol nonidat p40
    Enzyme Compatible Solvent System Isopropanol Nonidat P40, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme compatible solvent system isopropanol nonidat p40/product/Roche
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme compatible solvent system isopropanol nonidat p40 - by Bioz Stars, 2020-03
    84/100 stars
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