enzymatic digestion  (Worthington Biochemical)


Bioz Verified Symbol Worthington Biochemical is a verified supplier
Bioz Manufacturer Symbol Worthington Biochemical manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Collagenase Type 1
    Description:
    The original balance of enzymatic activities Each lot assayed for collagenase caseinase clostripain and tryptic activities Suggested for epithelial liver lung and adrenal primary cell isolations A dialyzed lyophilized powder
    Catalog Number:
    ls004194
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
    Buy from Supplier


    Structured Review

    Worthington Biochemical enzymatic digestion
    The original balance of enzymatic activities Each lot assayed for collagenase caseinase clostripain and tryptic activities Suggested for epithelial liver lung and adrenal primary cell isolations A dialyzed lyophilized powder
    https://www.bioz.com/result/enzymatic digestion/product/Worthington Biochemical
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Endothelial MT1‐ MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis
    Article Snippet: .. Briefly, after fat removal under a microscope, aortas were incubated for 5 min at 37°C in collagenase solution (collagenase type I, 3.33 mg/ml, Worthington, LS004194), thus allowing removal of the adventitia with forceps. .. The aortas were then cut into small pieces (1–2 mm), and a cell suspension was obtained by incubation for 45 min at 37°C in 6 mg/ml type I collagenase and 2.5 mg/ml elastase (Worthington, LS002290) diluted in DMEM.

    Gradient Centrifugation:

    Article Title: Decellularized extracellular matrices produced from immortal cell lines derived from different parts of the placenta support primary mesenchymal stem cell expansion
    Article Snippet: .. Following further digestion with 10mg/mL type 1 collagenase (Worthington) and DNase 1, the suspensions were passed through a 100 μm sieve and separated by density gradient centrifugation using Histopaque (Sigma-Aldrich, MO, USA). .. DMSCs were cultured in α-MEM medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS, Thermo Scientific, MA, USA), penicillin/streptomycin (100 U/mL and 100 mg/mL, respectively, Life Technologies), and 2mM L-glutamine (Sigma-Aldrich).

    Microscopy:

    Article Title: Endothelial MT1‐ MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis
    Article Snippet: .. Briefly, after fat removal under a microscope, aortas were incubated for 5 min at 37°C in collagenase solution (collagenase type I, 3.33 mg/ml, Worthington, LS004194), thus allowing removal of the adventitia with forceps. .. The aortas were then cut into small pieces (1–2 mm), and a cell suspension was obtained by incubation for 45 min at 37°C in 6 mg/ml type I collagenase and 2.5 mg/ml elastase (Worthington, LS002290) diluted in DMEM.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Worthington Biochemical enzymatic digestion
    Enzymatic Digestion, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/Worthington Biochemical
    Average 93 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results