Structured Review

STEMCELL Technologies Inc enzymatic digestion
Enzymatic Digestion, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzymatic digestion/product/STEMCELL Technologies Inc
Average 92 stars, based on 11 article reviews
Price from $9.99 to $1999.99
enzymatic digestion - by Bioz Stars, 2020-08
92/100 stars

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Lysis:

Article Title: Cryopreservation and in vitro culture of primary cell types from lung tissue of a stranded pygmy sperm whale (Kogia breviceps) ☆
Article Snippet: .. After enzymatic digestion, tissue was triturated with a P1000 Pipetman to break up clumps, pelleted at 450 g for 7 min, and resuspended in a 4:1 (v/v) mixture of NH4 Cl lysis buffer (StemCell Technologies Inc, Vancouver, BC, Canada) and HF (Hanks’ balanced Salt Solution, Invitrogen, supplemented with 2% FBS) to lyse red blood cells. .. The sample was then pelleted and resuspended with pipetting for 1 min in prewarmed 0.25% Trypsin-EDTA (Invitrogen, Carlsbad, CA, USA).

other:

Article Title: A Novel Animal Model for Locally Advanced Breast Cancer
Article Snippet: Enzymatic digestion was done with Gentle 10 × Collagenase/ Hyaluronidase (Stemcell Technologies) at 37°C overnight.

Article Title: Scribble is required for pregnancy-induced alveologenesis in the adult mammary gland
Article Snippet: All reagents for enzymatic digestion were purchased from StemCell™ Technologies.

Article Title: Human Non-Hematopoietic CD271pos/CD140alow/neg Bone Marrow Stroma Cells Fulfill Stringent Stem Cell Criteria in Serial Transplantations
Article Snippet: Spheres were passaged following enzymatic digestion with 0.25% type I collagenase (StemCell Technologies) for 30 min at 37°C, followed by washing with PBS, and replating at clonal density.

Article Title: Human Primary Bone Marrow Mesenchymal Stromal Cells and Their in vitro Progenies Display Distinct Transcriptional Profile Signatures
Article Snippet: Spheres were passaged following enzymatic digestion with 0.25% type I collagenase (StemCell Technologies) for 30 min at 37 °C, followed by washing with PBS, and re-plating at clonal density.

Article Title: Reduction of MHC-I expression limits T-lymphocyte-mediated killing of Cancer-initiating cells
Article Snippet: Single cell suspensions were prepared by crosshatching tumors with scalpel blades, followed by enzymatic digestion for one hour at 37 °C with collagenase/hyaluronidase (STEMCELL Technologies Inc., Vancouver, Canada).

Isolation:

Article Title: The assessment of CD146-based cell sorting and telomere length analysis for establishing the identity of mesenchymal stem cells in human umbilical cord
Article Snippet: .. MSC and EC isolation from UC tissue The whole UC tissue was mechanically dissected in small pieces (~0.2±0.1 g) and washed repeatedly with DPBS followed by enzymatic digestion using 600 U/ml collagenase I (#07902, Stem Cell Technologies, Grenoble, France) for 6 hours. .. Released cells were resuspended in 1:50 v/v DPBS, filtered through a 70 μm cell strainer (#352350, BD Biosciences, Oxford, UK), centrifuged (650g ) and counted.

Article Title: NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man
Article Snippet: .. TB-derived MSCs were isolated using enzymatic digestion with collagenase 0.25% (Stem Cell Technologies, Vancouver, Canada) for 4 hrs (37°C, 5% CO2 ). ..

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    STEMCELL Technologies Inc enzymatic digestion
    Enzymatic Digestion, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/STEMCELL Technologies Inc
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    92/100 stars
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