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Promega enzymatic digestion
Enzymatic Digestion, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzymatic digestion/product/Promega
Average 93 stars, based on 32 article reviews
Price from $9.99 to $1999.99
enzymatic digestion - by Bioz Stars, 2020-08
93/100 stars

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Related Articles

Positron Emission Tomography:

Article Title: Evaluation of a recombinant multiepitope antigen for diagnosis of hepatitis C virus: A lower cost alternative for antigen production, et al. Evaluation of a recombinant multiepitope antigen for diagnosis of hepatitis C virus: A lower cost alternative for antigen production
Article Snippet: .. After enzymatic digestion and gel purification of both plasmids, a synthetic gene was ligated to pET‐28a(+) plasmid using T4 DNA ligase kit following manufacturer's instructions (Promega, Madison, WI, USA). .. Chemically competent E. coli XL1‐Blue cells were transformed, plated in LB agar 50 μg/mL kanamycin, and incubated overnight at 37°C.

Isolation:

Article Title: A fully automatable enzymatic method for DNA extraction from plant tissues
Article Snippet: .. In order to follow the release of DNA at different times of enzymatic digestion, three 5 mm leaf disks from dry leaves of Ilex aquifolium were digested for 0.5 to 5 hours and the released DNA was isolated with the Wizard® Magnetic 96 Plant System kit (Promega). ..

Incubation:

Article Title: UV crosslinked mRNA-binding proteins captured from leaf mesophyll protoplasts
Article Snippet: .. For enzymatic digestion, gel pieces were covered with 25 μL of a digestion buffer [50 mM NH4 HCO3 , 5 mM CaCl2 , and 6 ng/μL trypsin (Promega)] and incubated on ice for 45 min. ..

Article Title: Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes
Article Snippet: .. Enzymatic digestion was performed with trypsin (1:100 enzyme to protein, V511A, Promega, Madison, WI) and 50 mM (NH4 )HCO3 and incubated at 37°C for 16 h. C18 ZipTips (Millipore) were used to enrich the tryptic peptides according to the manufacturer's instructions before proteomic analysis. .. Pre- and analytical columns were packed in-house with 3 μm C18 particles (Dalco Chromtech, Stockholm, Sweden) with 4 cm x 75 μm I.D. and 15 cm x 50 μm I.D., respectively.

Mass Spectrometry:

Article Title: Curcumin Cross-links Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Polypeptides and Potentiates CFTR Channel Activity by Distinct Mechanisms *
Article Snippet: .. Enzymatic digestion with trypsin (12.5 ng/μl) (Promega Gold Trypsin Mass Spectrometry Grade) was carried out for 16 h at 37 °C. ..

Plasmid Preparation:

Article Title: Evaluation of a recombinant multiepitope antigen for diagnosis of hepatitis C virus: A lower cost alternative for antigen production, et al. Evaluation of a recombinant multiepitope antigen for diagnosis of hepatitis C virus: A lower cost alternative for antigen production
Article Snippet: .. After enzymatic digestion and gel purification of both plasmids, a synthetic gene was ligated to pET‐28a(+) plasmid using T4 DNA ligase kit following manufacturer's instructions (Promega, Madison, WI, USA). .. Chemically competent E. coli XL1‐Blue cells were transformed, plated in LB agar 50 μg/mL kanamycin, and incubated overnight at 37°C.

Gel Purification:

Article Title: Evaluation of a recombinant multiepitope antigen for diagnosis of hepatitis C virus: A lower cost alternative for antigen production, et al. Evaluation of a recombinant multiepitope antigen for diagnosis of hepatitis C virus: A lower cost alternative for antigen production
Article Snippet: .. After enzymatic digestion and gel purification of both plasmids, a synthetic gene was ligated to pET‐28a(+) plasmid using T4 DNA ligase kit following manufacturer's instructions (Promega, Madison, WI, USA). .. Chemically competent E. coli XL1‐Blue cells were transformed, plated in LB agar 50 μg/mL kanamycin, and incubated overnight at 37°C.

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    Promega enzymatic digestion
    Enzymatic Digestion, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/Promega
    Average 93 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    93/100 stars
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